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公开(公告)号：CA1284415C
公开(公告)日：1991-05-28
申请号：CA525266
申请日：1986-12-12
Applicant: BIO RAD LABORATORIES
Inventor： SLEETER DONALD D , FERNWOOD GEORGE G , BURD SAMUEL
IPC: C08F2/10 , G01N27/26 , G01N27/447 , B29C39/24 , G01N27/28
Abstract: APPARATUS AND METHOD FOR GEL CASTING A gel used for electrophoresis is cast in a gel enclosure in which the gel will ultimately be used during the electrophoresis, by a novel method and using novel apparatus which provide for quick assembly and avoidance of leaks. The method and apparatus involve a wettable, preferably porous, membrane such as filter paper, which is thoroughly wetted with gel forming solution, then placed over the bottom opening of the gel enclosure and held in place until the gel on the membrane sets. Sealing of any cracks, gaps or irregularities along the rim of the opening is thereby achieved by the capillary action of the gel forming solution. The enclosure itself is then filled with gel forming solution. Once the latter has set, the membrane is removed.
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公开(公告)号：CA2004570A1
公开(公告)日：1990-06-02
申请号：CA2004570
申请日：1989-12-01
Applicant: BIO RAD LABORATORIES
Inventor： BURD SAMUEL
IPC: G01N27/447 , B01D57/02 , G01N27/453
Abstract: 12 APPARATUS FOR CAPILLARY ELECTROPHORESIS A capillary tube for use in high performance capillary electrophoresis is enclosed in a cartridge of generally planar configuration, formed by two flat plates joined together along raised edges to define a shallow enclosed chamber to retain the looped capillary tube, the plates having aligned windows between which a segment of the capillary tube passes, thereby forming a light path for zone detection. The termini of the capillary are embedded in fittings extending from the exterior of the cartridge, which mate with complementary fittings on inlet and outlet blocks which contain buffer reservoirs with controlled hydrostatic heads. The inlet block is specially equipped with ducts and fittings to permit filling of the capillary, injection of the sample into the capillary, changing of the solution in the buffer reservoir, and flushing out of the entire block without disconnecting the block from the capillary cartridge. By securing the cartridge containing the capillary tube into its holder, the capillary tube is correctly aligned in the detector light path.
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公开(公告)号：GB2164735B
公开(公告)日：1987-12-16
申请号：GB8514185
申请日：1985-06-05
Applicant: BIO RAD LABORATORIES
Inventor： FERNWOOD GEORGE GRAIL , BURD SAMUEL
IPC: G01N27/26 , F26B5/04 , F26B9/00 , G01N27/447 , F26B9/04
Abstract: An apparatus for drying gel slabs supplies heat to a horizontal gel slab from a heating plate above the slab, while drawing a vacuum beneath. The heating plate is weighted and has a flat lower surface at least coextensive with the gel slab. The slab itself is placed on a porous support pad in a vacuum chamber enclosed at the top with a flexible cover which clings to the gel and seals to the perimeter of the chamber when a vacuum is applied from below. The heating plate rests on top of the cover under gravitational force, providing even heating across the entire expanse of the gel slab and for the entire duration of the drying process, resulting in a continuous and uniform vapor flow downward from the gel slab, a minimization of internal stresses, and reduced susceptibility of the gel to cracking.
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公开(公告)号：CA1210309A
公开(公告)日：1986-08-26
申请号：CA455733
申请日：1984-06-01
Applicant: BIO RAD LABORATORIES
Inventor： FERNWOOD GEORGE G , BURD SAMUEL
Abstract: 12 DUAL PURPOSE BIOCHEMICAL TEST PLATE ASSEMBLY A biochemical test plate assembly is disclosed for use in both filter assays and static blotting applications. The assembly comprises an upper template with a plurality of discrete apertures, a continuous microporous film underlying the upper template, a gasket underlying the microporous film with apertures matching those of the upper template, a lower template underlying the gasket with a similar array of apertures matching those of both the upper template and the gasket, each aperture terminating at its upper end in a flat boss, and a base plate having a central recess to define an enclosed chamber when the base plate and lower template are in contact, the base plate being designed to form an air-tight seal between it and the power template.
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公开(公告)号：CA1263230A
公开(公告)日：1989-11-28
申请号：CA480505
申请日：1985-05-01
Applicant: BIO RAD LABORATORIES
Inventor： FERNWOOD GEORGE G , BURD SAMUEL
Abstract: METHOD AND APPARATUS FOR DRYING GEL SLABS An apparatus for drying gel slabs supplies heat to a horizontal gel slab from a heating plate above the slab, while drawing a vacuum beneath. The heating plate is weighted and has a flat lower surface at least coextensive with the gel slab. The slab itself is placed on a porous support pad in a vacuum chamber enclosed at the top with a flexible cover which clings to the gel and seals to the perimeter of the chamber when a vacuum is applied from below. The heating plate rests on top of the cover under gravitational force, providing even heating across the entire expanse of the gel slab and for the entire duration of the drying process, resulting in a continuous and uniform vapor flow downward from the gel slab, a minimization of internal stresses, and reduced susceptibility of the gel to cracking.
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Patent Agency Ranking
公开(公告)号：GB2184238A
公开(公告)日：1987-06-17
申请号：GB8619267
申请日：1986-08-07
Applicant: BIO RAD LABORATORIES
Inventor： SLEETER DONALD D , FERNWOOD GEORGE G , BURD SAMUEL
IPC: C08F2/10 , G01N27/26 , G01N27/447 , G01N27/28
Abstract: A gel used for electrophoresis is cast in a gel enclosure in which the gel will ultimately be used during the electrophoresis, by a novel method and using novel apparatus which provide for quick assembly and avoidance of leaks. The method and apparatus involve a wettable, preferably porous, membrane such as filter paper, which is thoroughly wetted with gel forming solution, then placed over the bottom opening of the gel enclosure and held in place until the gel on the membrane sets. Sealing of any cracks, gaps or irregularities along the rim of the opening is thereby achieved by the capillary action of the gel forming solution. The enclosure itself is then filled with gel forming solution. Once the latter has set, the membrane is removed.
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公开(公告)号：DE3521680A1
公开(公告)日：1986-03-27
申请号：DE3521680
申请日：1985-06-18
Applicant: BIO RAD LABORATORIES
Inventor： FERNWOOD GEORGE G , BURD SAMUEL
Abstract: An apparatus for drying gel slabs supplies heat to a horizontal gel slab from a heating plate above the slab, while drawing a vacuum beneath. The heating plate is weighted and has a flat lower surface at least coextensive with the gel slab. The slab itself is placed on a porous support pad in a vacuum chamber enclosed at the top with a flexible cover which clings to the gel and seals to the perimeter of the chamber when a vacuum is applied from below. The heating plate rests on top of the cover under gravitational force, providing even heating across the entire expanse of the gel slab and for the entire duration of the drying process, resulting in a continuous and uniform vapor flow downward from the gel slab, a minimization of internal stresses, and reduced susceptibility of the gel to cracking.
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公开(公告)号：GB2502409B8
公开(公告)日：2014-10-15
申请号：GB201303939
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：GB2504241B
公开(公告)日：2014-03-12
申请号：GB201319376
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE JR , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MARKAREWICZ ANTHONY JOSEPH
Abstract: A system for sample analysis, comprising:at least one droplet generator that forms a plurality of emulsions including droplets of partitioned samples, the samples being prepared as reaction mixtures for amplification of a nucleic acid target;a plate defining an array of cavities to hold the emulsions;a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets of the emulsions;a detection assembly to detect signals from intact droplets of the emulsions;a fluid transfer device including an autosampler configured to transfer droplets from each of the cavities to a detection site of the detection assembly; anda controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in the samples based on signals detected from the intact droplets.
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公开(公告)号：CA3149293A1
公开(公告)日：2010-04-01
申请号：CA3149293
申请日：2009-09-23
Applicant: BIO RAD LABORATORIES
Inventor： COLSTON BILLY WAYNE , HINDSON BENJAMIN JOSEPH , NESS KEVIN DEAN , MASQUELIER DONALD ARTHUR , MILANOVICH FRED PAUL , MODLIN DOUGLAS N , RIOT VINCENT , BURD SAMUEL , MAKAREWICZ ANTHONY JOSEPH , BELGRADER PHILLIP
Abstract: A method of analysis which involves selecting a device having a port connected to a chamber and placing a sample-containing fluid into the port. The method also involves creating a pressure differential that drives the sample-containing fluid from the port to the chamber and separates the sample-containing fluid into droplets, forming a two-dimensional monolayer of the droplets in the chamber, and imaging at least a portion of the monolayer.
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