公开(公告)号：WO2010028366A2

公开(公告)日：2010-03-11

申请号：PCT/US2009056225

申请日：2009-09-08

Applicant: LIFE TECHNOLOGIES CORP ,  GREINER DOUGLAS P ,  GJERSTAD CARMEN ,  ZIEGLE JANET S ,  JONES LEE W ,  SHEN MIN-YI ,  CHIN JASON ,  BREU HEINZ

Inventor： GREINER DOUGLAS P ,  GJERSTAD CARMEN ,  ZIEGLE JANET S ,  JONES LEE W ,  SHEN MIN-YI ,  CHIN JASON ,  BREU HEINZ

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12Q2545/101 ,  C12Q2563/149

Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.

Abstract translation: 公开了一种用于执行核酸样品测序的质量控制的系统。 该系统具有一组固体支持物，每个支持物附着有多个核酸序列。 该组具有多组固体支持物，并且每组包含具有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成来源的。 还公开了制备用于执行核酸样品测序的质量控制的方法和验证核酸测序仪器的方法。

公开(公告)号：SG11201606778TA

公开(公告)日：2016-09-29

申请号：SG11201606778T

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORP

Inventor： GJERSTAD CARMEN ,  DONG SHOULIAN

IPC: C12Q1/68

Abstract: A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.

公开(公告)号：AU2023300374A1

公开(公告)日：2025-01-16

申请号：AU2023300374

申请日：2023-06-29

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SHANNON MARK ,  GJERSTAD CARMEN ,  LEONG HARRISON ,  ZAHEDI MOHAMMAD MEHDI ,  EVANS BRIAN ,  MULLAH KHAIRUZZAMAN BASHAR

IPC: C12Q1/6816 ,  C09B11/24 ,  C12Q1/686

Abstract: Disclosed are compositions, kits, and methods that enable intra-channel multiplexing by enabling determination of separate detectable signals, each associated with a different assay target, within the same detection channel. The multiple detectable signals can be separately resolved and independently analyzed to enable detection and/or quantification of each respective target. Enabling multiple targets to be assayed within the same detection channel increases the plexy of multiplex assays without relying on additional dyes and concomitant issues of increased spectral overlap.

公开(公告)号：AU2023298557A1

公开(公告)日：2025-01-16

申请号：AU2023298557

申请日：2023-06-29

Applicant: LIFE TECHNOLOGIES CORP

Inventor： SHANNON MARK ,  GJERSTAD CARMEN ,  LEONG HARRISON

IPC: C12Q1/6816 ,  G16B25/20

Abstract: Systems and methods that enable analyte detection in a multiplexed amplification process can include obtaining, at multiple time points during the amplification process, composite emission signal data associated with a composite emission signal from at least a first probe type comprising a first label configured to generate a first emission signal and a second probe type comprising a second label configured to generate a second emission signal which has spectrally similar characteristics as said first emission signal, the first probe type and the second probe type differing in thermal and/or temporal properties; and determining, based at least partially on the composite emission signal data, emission signal data associated with a emission signal from a given probe type of the first probe type or the second probe type during the amplification process.

公开(公告)号：EP2344678A4

公开(公告)日：2012-06-06

申请号：EP09812376

申请日：2009-09-08

Applicant: LIFE TECHNOLOGIES CORP

Inventor： GREINER DOUGLAS P ,  GJERSTAD CARMEN ,  ZIEGLE JANET S ,  JONES LEE W ,  SHEN MIN-YI ,  CHIN JASON ,  BREU HEINZ

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12Q2545/101 ,  C12Q2563/149