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公开(公告)号：JPH1144672A
公开(公告)日：1999-02-16
申请号：JP20318797
申请日：1997-07-29
Applicant: TOA ELECTRONICS
Inventor： KONNO SO , KATO EIJI , TSUKADA YUICHI , HAKETA YASUSHI , WADA YUKIHIRO
Abstract: PROBLEM TO BE SOLVED: To obtain an analytical method in which vitamin C can be quantitatively determined precisely by removing the effect of reducing organic substances even when the reducing organic substances other than the vitamin C exist in a sample liquid by a method wherein the sample liquid is brought into contact with a functional group which comprises a distribution capability or an adsorption capability. SOLUTION: In an analytical method for vitamin C by coulometery, a sample liquid which contains the vitamin C is supplied to a detecting cell in which a detecting electrode composed of a conductive porous body and a counter electrode are arranged via a diaphragm, and the vitamin C is quantitatively determined by an electrolytic operation. Then, the sample liquid is brought into contact with a substance into which a functional group comprising a distribution capability or an adsorption capability is introduced, and it is supplied to the detecting cell so as to be quantitatively determined. That is to say, the sample liquid is brought into contact with the substance into which the functional group comprising the distribution capability or the adsorption capability is introduced so as to be pretreated, and reducing organic substances, other than the vitamin C, which are contained in the sample liquid can be removed mostly. A result which is obtained by the chlorometry agrees well with the concentration of vitamin C measured by an indophenol titration method as an official method.
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公开(公告)号：JPH10165200A
公开(公告)日：1998-06-23
申请号：JP34058196
申请日：1996-12-05
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , KOBAYASHI KANEO
Abstract: PROBLEM TO BE SOLVED: To provide a method for assaying microbial ATP, capable of efficiently and accurately conducting a microbial assay for a sample rich in free ATP in a short time. SOLUTION: This method for microbial ATP assay is characterized by that an ATP-decomposing reagent is added to a sample rich in free ATP not derived from microorganism to decompose the free ATP in the sample followed by adding an ATP-extractive reagent to the resultant sample to extract ATP from the microorganisms. At this time, simultaneously, the extractive reagent deactivates the ATP-decomposing reagent added to the sample previously, and subsequently, a luminescent reagent containing a reagent suppressing the inhibition of an enzymatic reaction is brought into contact with the resultant sample, and the light generated by biological chemiluminescent reaction is assayed with a relevant device.
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3.发明专利
公开(公告)号：JPH03123481A
公开(公告)日：1991-05-27
申请号：JP26170989
申请日：1989-10-06
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , MOTOHASHI RYOICHI , GONDA KINJI , KAJIWARA KAZUTO
Abstract: PURPOSE:To determine the cell concentration and cell activity in a cell suspension liquid at the same time by placing a chemiluminescence detector in a detection channel and an absorbance detector in a sample supplying channel, etc., mixing a sample supplied through a sample channel and a reagent supplied through a reagent channel in a mixer and transferring the mixture through the detection channel. CONSTITUTION:A sample liquid S containing suspended cells in injected into a carrier liquid C1 and an ATP extraction liquid R is injected into the carrier liquid before a mixer 11. A luminescent reagent E containing luciferin and luciferase is injected into a carrier liquid C2 and the sample liquid is mixed to the reagent liquid with a mixer 12. A sample channel 2 is joined to a reagent channel 4 and the mixture is passed through the mixer 13 to a chemiluminescence detector 6 and an absorbance detector 7 to determine the ATP concentration and turbidity. The ATP concentration in the cell and the concentration outside of the cell can be separately determined by injecting the ATP extraction liquid R and without using the injection of the liquid.
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公开(公告)号：JPH11108917A
公开(公告)日：1999-04-23
申请号：JP28284497
申请日：1997-09-30
Applicant: TOA ELECTRONICS
Inventor： KATO EIJI , HAKETA YASUSHI , SATO TETSUYA
Abstract: PROBLEM TO BE SOLVED: To obtain a titration control method in which a titration reagent is dropped always at proper intervals and in which a titration time is shortened by a method wherein the magnitude of a waiting time required for a detection by a sensor is controlled according to the magnitude of a change in the chemical quantity or the physical quantity of a solution to be inspected. SOLUTION: A pH electrode 3 as a sensor and a reference electrode 4 are inserted into a container 2 in which a solution L, to be inspected, at an automatic titration apparatus 1 is housed, and the respective electrodes 3, 4 are connected to an apparatus body 7 by respective lead wires 5, 6. Then, a titration reagent A is dropped, by every small amount, into the solution L, to be inspected, from a syringe 10, and a change in the chemical quantity or the physical quantity of the solution L to be inspected is measured by the sensor. At this time, according to the magnitude of the change in the chemical quantity or the physical quantity of the solution L, to be inspected, after a prescribed waiting time has elapsed since the titration reagent A is dropped, the magnitude of a waiting time required for a detection by the sensor after the titration reagent A is dropped next is controlled. Thereby, even when the change becomes large near the end point of a titrating operation, the end point can be detected surely, and the titrating operation can be performed with high accuracy.
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公开(公告)号：JPH1189592A
公开(公告)日：1999-04-06
申请号：JP28284597
申请日：1997-09-30
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , KOBAYASHI KANEO
Abstract: PROBLEM TO BE SOLVED: To provide a mensuration of ATP of microorganisms enabling to accurately measure the ATP of microorganism of a sample in biochemiluminescence by decomposing only free ATP in the sample containing free ATP in plenty such as foods, etc., utilizing ATP catabolic enzyme. SOLUTION: The microorganism contained in the sample is collected on a filter maternal by filtering sample containing the ATP decomposing reagent after decomposing free ATP in the sample containing microorganism by adding ATP decomposing reagent. The sample solution dissolving ATP decomposing reagent adhering on filtering material and the microorganism is removed by washing the filtering material with aseptic cleaning fluid. The ATP is extracted from the cells of the microorganism by applying an extracting reagent to the microorganism collected on the filtering material and the concentration of the extracted ATP of the microorganism is measured in biochemiluminescence using luciferase and luciferin.
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Patent Agency Ranking
公开(公告)号：JPH08257318A
公开(公告)日：1996-10-08
申请号：JP9183795
申请日：1995-03-24
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , IBUKI TADAHIRO
Abstract: PURPOSE: To provide an inexpensive filter capable of perfectly eliminating the contamination with various bacteria between samples even when a large number of samples are examined, enhanced in work efficiency and used in the examination of bacteria. CONSTITUTION: A filter 1 has a membrane filter M and a filter stand 20 holding a porous filter support 30 capable of holding the filter membrane M on the upper surface thereof in a freely detachable manner. The filter stand 10 is held on a filter stand holder 10 in a freely detachable manner. A holder 40 for storing a sample is attached to the filter stand holder 10 in a freely detachable manner through the filter stand 20.
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7.发明专利
公开(公告)号：JPH05126819A
公开(公告)日：1993-05-21
申请号：JP31839691
申请日：1991-11-06
Applicant: TOA ELECTRONICS , MORINAGA MILK INDUSTRY CO LTD
Inventor： HAKETA YASUSHI , SHINNO TETSUHISA , MOTOHASHI RYOICHI , KANZAKI MIKIO , WATANABE HIROTAKA , FUJIOKA SEIICHI
Abstract: PURPOSE:To obtain the method and the apparatus for measuring the acidity, the L-lactic-acid concentration and the D-lactic-acid concentration of fermented milk at the same time. CONSTITUTION:The acidity of the sample, which is sampled from fermented milk, is measured with an acidity measuring device having a conductivity electrode 24 and the like. The L-lactic-acid concentration of the sample is measured with an L-lactic-acid-concentration measuring device having an L-lactic-acid electrode 34 and the like. The added amount of the L-lactic-acid concentration of the fermented milk, which is measured with the L-lactic-acid concentration measuring device, and the acidity of raw material milk, which is measured beforehand, is subtracted from the acidity of the fermented milk, which is measured with the acidity measuring device, with a computer device 38, which is connected to the acidity measuring device and the L-lactic-acid concentration measuring device. Thus, the D-lactic-acid concentration of the fermented milk is computed and obtained.
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公开(公告)号：JPH07203995A
公开(公告)日：1995-08-08
申请号：JP2381094
申请日：1994-01-26
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , NISHINO TATSUO , TSUNODA HIROSHI
Abstract: PURPOSE:To quickly determine intracellular ATP for microbial examination, etc., in high sensitivity without diluting the specimen by contacting an extracted specimen of intracellular ATP with an agent for suppressing the inhibition of enzymatic reaction and containing cyclodextrin and applying a biochemical luminescent method to the contact product. CONSTITUTION:ATP in a cell is determined in high sensitivity for food sanitation, clinical examination, environmental microbial examination, etc., by contacting a specimen containing cells of microorganisms, etc., with an adenosine triphosphate (ATP) extraction reagent containing a surfactant (e.g. dodecyldimethylbenzyl ammonium chloride) to extract the intracellular ATP of the specimen, contacting the extracted specimen with an agent for suppressing the inhibition of enzymatic reaction and containing a cyclodextrin (alpha- cyclodextrin), adding a luminescent reagent containing luciferin as a fluorescent substrate and luciferase as a luminescent enzyme to the extracted specimen to apply biochemical luminescent method and measuring the light emitted by the biochemical luminescence caused by the enzymatic reaction of luciferin, luciferase and ATP.
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公开(公告)号：JPH07110301A
公开(公告)日：1995-04-25
申请号：JP27897493
申请日：1993-10-12
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , NISHINO TATSUO , TSUNODA HIROSHI
Abstract: PURPOSE:To provide a method and apparatus for measuring ATP quantity of cell which enables the ATP (adenosine triphosphate) quantity of cells to be measured with high precision dispensing with the operation of folding a filter membrane after cell trap and not only improving operability but also solving the dangerousness of contamination from outside the filter membrane. CONSTITUTION:A filter membrane 100 which has trapped cells in a specimen is placed in a one-sided open tray container 200 without being folded. The container 200 is injected with an extraction reagent 104 and a luminous reagent to cause biochemical luminous reaction. A photomultiplier tube 108 is installed so as to face the opening of this container 200 to detect the luminous quantity, resulting in measurement of the ATP quantity of cells.
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公开(公告)号：JPH0530998A
公开(公告)日：1993-02-09
申请号：JP32386291
申请日：1991-11-11
Applicant: TOA ELECTRONICS
Inventor： HAKETA YASUSHI , MOTOHASHI RYOICHI
Abstract: PURPOSE:To carry out a sensitive measurement of intracellular ATP, suitable for a food sanitation test, etc., while reducing inhibition of the enzyme caused by a surfactant by bringing a cell-containing sample into contact with an ATP- extracting reagent and an enzymatic reaction inhibitor and measuring ATP by using the biochemical luminescence method. CONSTITUTION:A cell-containing sample solution 4 is led through a passage 22 for the sample solution to an injector 14 by a pump 10. A carrier solution 3 containing an ATP-extracting reagent for extracting intracellular adenosine triphosphate (ATP) and an enzymatic reaction inhibitor is then led to the injector 14 by a pump 9 so as to be brought into contact with the sample solution 4 for the purpose of extraction of the intracellular ATP. The sample solution 4 is subsequently led through a mixer 17 to a mixing passage 28 where the sample solution 4 is joined with a luciferin and luciferase-containing luminous reagent 2 supplied through a mixer 16 by an injector 13 so as to generate light by a biochemical reaction. The amount of the generated light is detected by a chemiluminescence detector 19 and then compared with data of known concentration samples by using a computer 20, thus measuring the concentration of ATP contained in the sample.
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