-
公开(公告)号：KR1020130049754A
公开(公告)日：2013-05-14
申请号：KR1020120123842
申请日：2012-11-02
Applicant: 성균관대학교산학협력단
CPC classification number: C12Q1/6844 , C12Q1/04 , C12Q2527/101
Abstract: PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting tobacco leaf curl virus is provided to early detect the virus, and to prevent economic loss. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting tobacco leaf curl virus has sequence numbers 1-4. The prime set contains sequence numbers 17-20. The virus is selected from the group consisting of: NCBI No. HM164547, HM164541, HM164545, and HM164550 mutant strains found in Korea; and NCBI No. EF620536, NC_004641, NC_004654, AB287439, AB028604, AB055008, AB055009, and AB079689 mutant strains found in Japan. A composition contains DNA polymerase for loop-mediated isothermal amplification reaction, dNTPs, and a reaction buffer.
Abstract translation: 目的：提供用于检测烟叶卷曲病毒的环介导等温扩增反应的底物组合物，用于早期检测病毒，防止经济损失。 构成：用于检测烟草卷曲病毒的环介导等温扩增反应的引物组具有序列号1-4。 素数集合包含序号17-20。 该病毒选自在韩国发现的NCBI No.HM164547，HM164541，HM164545和HM164550突变株; 和NCBI No.EEF620536，NC_004641，NC_004654，AB287439，AB028604，AB055008，AB055009和在日本发现的AB079689突变株。 组合物含有用于环介导的等温扩增反应的DNA聚合酶，dNTP和反应缓冲液。
-
公开(公告)号：KR101090459B1
公开(公告)日：2011-12-07
申请号：KR1020090035785
申请日：2009-04-24
Applicant: 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
Abstract: 본 발명은 전사 후 유전자 침묵 현상의 억제 활성을 갖는 국내 고구마 잎말림병 감염 ＤＮＡ 바이러스 유래 유전자에 관한 것으로서, 더욱 상세하게는 저항성 식물을 개발하거나, 외래 유전자의 발현을 위해 식물의 방어 기작을 무력화하는 인자로써의 적용 가능하고, 또한 식물 내 특정 유전자의 도입 시 전달체의 효율 증대를 위해 이용될 수 있는 국내 고구마 잎마름병 감염 DNA 바이러스 유래 AC2 유전자에 관한 것이다.
고구마 잎말림병 감염 DNA 바이러스, 전사 후 유전자 침묵, AC2 유전자-
公开(公告)号：KR1020090112600A
公开(公告)日：2009-10-28
申请号：KR1020090036080
申请日：2009-04-24
Applicant: 성균관대학교산학협력단
CPC classification number: C07K14/005 , C12N15/8205 , C12N15/8216 , C12N15/8241
Abstract: PURPOSE: A recombinant HYVV gene using Korean Honeysuckle yellow vein virus and its recombinant virus vector are provided to obtain genetic resource of HYVV gene which causes large industrial damage. CONSTITUTION: A recombinant HYVV gene using Korean Honeysuckle yellow vein virus and its recombinant virus vector is recombinant HYVV gene containing sequence of the sequence number 1 or 2 isolated from Honeysuckle yellow vein virus. The partial gene sequence of virus of single stranded DNA in a plant is confirmed by polymerase chain reaction. The plant is a transformed plant of tobacco or rice plant.
Abstract translation: 目的：提供使用韩国金银花黄色静脉病毒的重组HYVV基因及其重组病毒载体，以获得导致大量工业损伤的HYVV基因遗传资源。 构成：使用韩国金银花黄色静脉病毒的重组HYVV基因及其重组病毒载体是含有从金银花黄色静脉病毒分离的序列号1或2的序列的重组HYVV基因。 通过聚合酶链反应证实植物中单链DNA病毒的部分基因序列。 该植物是烟草或水稻植物的转化植物。
-
公开(公告)号：KR1020090112492A
公开(公告)日：2009-10-28
申请号：KR1020080038415
申请日：2008-04-24
Applicant: 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
Abstract: PURPOSE: A bidirectional promoter of sweet potato leaf curl infection DNA virus is provided to produce vector product in molecular biology field and molecular breeding industry. CONSTITUTION: A bidirectional promoter of sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 1. A method for identifying the promoter activity comprises: a step of isolating a site with promoter activity and analyzing cis-acting regulation site; a step of recombining promoter site by using gateway cloning to a plant expression vector for promoter analysis; a step of transforming the recombined promoter site to Agrobacteria tumefaciens strain GV3101; and a step of screening transformant.
Abstract translation: 目的：提供甘薯叶卷曲感染DNA双向启动子，在分子生物学领域和分子育种工业中产生载体产物。 构成：甘薯叶卷曲感染DNA病毒的双向启动子由序列号1的序列表示。鉴定启动子活性的方法包括：分离具有启动子活性的位点并分析顺式作用调节位点的步骤; 通过使用网关克隆到用于启动子分析的植物表达载体来重组启动子位点的步骤; 将重组的启动子位点转化到根癌土壤杆菌菌株GV3101的步骤; 并筛选转化体的步骤。
-
-
-
Search Results
94
records
(took 0.023 seconds)
