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    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS
    101.
    发明专利
    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS 未知

    公开(公告)号：CA2615315A1

    公开(公告)日：2007-01-25

    申请号：CA2615315

    申请日：2006-07-18

    Applicant: BASF AG

    Inventor： YOCUM R ROGERS ,  SCHRODER HARTWIG ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  WILLIAMS MARK K ,  ZELDER OSKAR

    IPC: C12P13/12

    Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &Dgr;metF organism or a &Dgr;metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &Dgr;metF &Dgr;metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    102.
    发明专利
    未知

    公开(公告)号：NO20062685L

    公开(公告)日：2006-09-14

    申请号：NO20062685

    申请日：2006-06-09

    Applicant: BASF AG

    Inventor： KROGER BURKHARD ,  ZELDER OSKAR ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH ,  KLOPPROGGE CORINNA ,  HEINZLE ELMAR

    IPC: C12P13/08 ,  C12N9/04

    Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

    MULTIPLE PROMOTERS AND THE USE THEREOF FOR GENE EXPRESSION
    105.
    发明专利
    MULTIPLE PROMOTERS AND THE USE THEREOF FOR GENE EXPRESSION 未知

    公开(公告)号：CA2591311A1

    公开(公告)日：2006-07-06

    申请号：CA2591311

    申请日：2005-12-21

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG

    IPC: C12N15/77 ,  C07K14/34

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

    106.
    发明专利
    未知

    公开(公告)号：DE102004035065A1

    公开(公告)日：2006-02-16

    申请号：DE102004035065

    申请日：2004-07-20

    Applicant: BASF AG

    Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  KROEGER BURKHARD ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

    IPC: C12N1/00 ,  C12N15/11 ,  C12N15/67 ,  C12P13/04

    Abstract: A specific nucleic acid (I) with promoter activity is used for transcribing genes, where (I) is: (a) a sequence (1) of 178 nucleotides (reproduced); (b) a derivative of (1) with >= 90% identity, formed by substitution, insertion or deletion; (c) a sequence that hybridizes to (1) under stringent conditions; or (d) a functional fragment of (a)-(c). Independent claims are also included for: (1) use of an expression unit (EU), containing and linked to a nucleic acid (NA) that ensures translation of RNA, for expressing genes; (2) (I), except sequence (1) itself, as new compounds and EU containing it; (3) method for altering (or causing) the transcription (or expression) rate of genes in microorganisms relative to the wild type; (4) expression cassette (EC) comprising EU, at least one other functionally linked NA to be expressed and optionally other genetic control elements that are heterologous with respect to EU; (5) expression vector containing EC; (6) genetically modified microorganism (GMM) in which the transcription rate of at least one gene is altered (or caused) relative to the wild type; (7) GMM containing EU and a functionally linked gene to be expressed, where this is heterologous with respect to EU; (8) preparation of biosynthetic products (A) by culturing the GMM of (6) or (7); (9) use of the sequence aggagga (21) as ribosome-binding site in expression units for expressing genes in Corynebacterium or Brevibacterium; (10) use of the sequences ttaatt (19) or taagct (20) as -10 regions in EU for expressing genes in Corynebacterium or Brevibacterium; and (11) EU that contain (21) or at least one of (19) or (20).

    PGRO EXPRESSION UNITS
    107.
    发明专利
    PGRO EXPRESSION UNITS 未知

    公开(公告)号：CA2548306A1

    公开(公告)日：2005-06-30

    申请号：CA2548306

    申请日：2004-12-15

    Applicant: BASF AG

    Inventor： KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR

    IPC: C12N15/77 ,  C07K14/34 ,  C12P13/08

    Abstract: The invention relates to the use of nucleic acid sequences for regulating ge ne transcription and expression, said novel promoters and expression units, methods for modifying or inducing the gene transcription rate and/or expression rate, expression cassettes containing said expression units, genetically modified microorganisms having a modified or induced transcripti on rate and/or expression rate, and methods for producing biosynthetic products by cultivating said genetically modified microorganisms.

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