公开(公告)号：AU2007233270B2

公开(公告)日：2013-06-20

申请号：AU2007233270

申请日：2007-03-02

Applicant: FLUIDIGM CORP

Inventor： WEST JAY A A ,  SATTERFIELD BRENT C

IPC: C12Q1/68

Abstract: The present invention provides inter alia, cooperative probe assays for analyzing and identifying biological substances.

公开(公告)号：AT541946T

公开(公告)日：2012-02-15

申请号：AT08829141

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: C12Q1/68

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：IL215462D0

公开(公告)日：2011-12-29

申请号：IL21546211

申请日：2011-10-02

Applicant: FLUIDIGM CORP

IPC: C12Q20060101

Abstract: The present invention provides an elastomer microfluidic device that enables simultaneous amplification of multiple nucleic acid samples with multiple pairs of primers in a matrix format as well as recovery of the products e.g. for next generation sequencing. The flow of samples and reagents as well as the compartmentalization of individual reactions in the multiple channels and chambers is controlled using valves and dilation pumping / volumetric capacitive pumping.

公开(公告)号：CA2786916A1

公开(公告)日：2011-07-21

申请号：CA2786916

申请日：2011-01-14

Applicant: UNIV BRITISH COLUMBIA ,  FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  HEYRIES KEVIN ,  HANSEN CARL L G ,  PETRIV OLEH

IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  G01N33/50

Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.

公开(公告)号：CA3018687A1

公开(公告)日：2010-10-07

申请号：CA3018687

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  KAPER FIONA ,  MAY ANDREW ,  WANG JUN

IPC: G01N33/48 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12Q1/6853

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：AU2010214783A1

公开(公告)日：2010-09-23

申请号：AU2010214783

申请日：2010-09-02

Applicant: FLUIDIGM CORP

Inventor： GROSSMAN ROBERT ,  UNGER MARC ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN

IPC: G01N15/06 ,  B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58 ,  G01N33/00 ,  G01N33/48

Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

公开(公告)号：AU2005208879B2

公开(公告)日：2010-06-03

申请号：AU2005208879

申请日：2005-01-25

Applicant: FLUIDIGM CORP

Inventor： CHOU HOU-PU ,  GROSSMAN ROBERT ,  LAM PHILLIP ,  KIMBALL JAKE ,  PIEPRZYK MARTIN ,  UNGER MARC

IPC: G01N15/06 ,  B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58 ,  G01N33/00 ,  G01N33/48

Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

公开(公告)号：SG145697A1

公开(公告)日：2008-09-29

申请号：SG2008056475

申请日：2004-07-28

Applicant: FLUIDIGM CORP

Inventor： TAYLOR COLIN JON ,  SUN GANG ,  DUBE SIMANT

IPC: B01L3/00 ,  B29C20060101 ,  C12Q1/68 ,  G06K9/00 ,  G06K9/62 ,  G06T7/00

Abstract: IMAGE PROCESSING METHOD AND SYSTEM FOR MICROFLUIDIC DEVICES A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed formed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

公开(公告)号：DE60120230D1

公开(公告)日：2006-07-06

申请号：DE60120230

申请日：2001-11-30

Applicant: FLUIDIGM CORP

Inventor： FERNANDES N ,  CHOU HOU-PU ,  UNGER A

IPC: F15C5/00 ,  B01L3/00 ,  F15C3/04 ,  F16K99/00 ,  G06F17/50

公开(公告)号：AU2004240944A1

公开(公告)日：2004-12-02

申请号：AU2004240944

申请日：2004-05-20

Applicant: FLUIDIGM CORP

Inventor： TAYLOR COLIN JON ,  CEASAR CHRISTOPHER ,  SUN GANG ,  QUAN EMERSON CHUENG ,  HARRIS GREG ,  LEE MICHAEL

IPC: B29D11/00 ,  B44C1/22 ,  C02F1/40 ,  F15C1/04 ,  F15C1/06 ,  G01N21/00 ,  G01N33/558 ,  G01N35/02 ,  B01L3/00

Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.