公开(公告)号：AU2011228213B2

公开(公告)日：2014-10-09

申请号：AU2011228213

申请日：2011-03-14

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  MINAMINO ATSUSHI ,  YAMAMOTO YUKI ,  YAMADA KATSUSHIGE

IPC: C12P19/14 ,  C12M1/00

Abstract: A method for manufacturing a sugar solution by repeating a sugar solution manufacturing process comprising steps (1) - (3) below, said manufacturing process being characterised by the use of a recovered saccharifying enzyme obtained during step (3) in step (1) of subsequent sugar solution manufacturing processes. Step (1): cellulose undergoes primary hydrolysis by adding a cellulase from a filamentous fungus thereto. Step (2): unused cellulase from the filamentous fungus is added to the hydrolysis product from step (1) and a secondary hydrolysis is carried out. Step (3): the hydrolysis product from step (2) undergoes solid-liquid separation, and a recovered enzyme is obtained from the acquired sugar solution. Thus provided is a method for reducing the amount of an enzyme such as cellulase used in a method for manufacturing a sugar solution from a pretreated cellulose material.

公开(公告)号：AU2013208441A1

公开(公告)日：2014-08-21

申请号：AU2013208441

申请日：2013-01-11

Applicant: TORAY INDUSTRIES

Inventor： ISOBE KYOHEI ,  WATANABE SHIOMI ,  KOBAYASHI KOJI ,  SAWAI KENJI ,  NA KYUNGSU ,  HIRAMATSU SHINGO ,  YAMADA KATSUSHIGE

IPC: C12P7/06 ,  C12P7/18 ,  C12P7/56

Abstract: [Problem] To provide a method for producing a chemical substance with high yield using a mixed sugar composed of a hexose and a pentose as a fermentation raw material. [Solution] Provided is a method for producing a chemical substance through continuous fermentation, said method comprising filtrating a culture solution of a microorganism using a separation membrane, retaining an unfiltered portion of the culture solution in the culture solution or refluxing the unfiltered portion through the culture solution, adding a new fermentation raw material to the culture solution and collecting a product from the culture solution, wherein the fermentation raw material comprises a pentose and a hexose and the microorganism has a pathway through which a pentose can be metabolized with a pentose isomerase.

公开(公告)号：AU2012281960A1

公开(公告)日：2014-02-27

申请号：AU2012281960

申请日：2012-01-13

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  ISHIKAWA KAZUHIKO ,  MISHIMA YUMIKO ,  WADA YASUNOBU ,  KADO YUJI

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/42 ,  C12P19/14

Abstract: Provided is a novel &bgr;-glucosidase which can exhibit a high activity in the presence of a biomass and has high thermal stability to conventional enzymes. A &bgr;-glucosidase characterized by being produced by substituting and/or deleting at least three amino acid residues selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227 and Glu330 in the amino acid sequence for a parent &bgr;-glucosidase by another amino acid residue, and exhibiting a cellobiose decomposition activity.

公开(公告)号：ES2436567T3

公开(公告)日：2014-01-03

申请号：ES10783384

申请日：2010-06-02

Applicant: TORAY INDUSTRIES

Inventor： SAWAI KENJI ,  SUDA KAZUMI ,  SAWAI HIDEKI ,  YAMADA KATSUSHIGE ,  YAMAGISHI JUNYA

IPC: C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N9/04 ,  C12P7/56

Abstract: Polipéptido que es cualquiera de los siguientes (A) a (C): (A) polipéptido que tiene la secuencia de aminoácidos que se muestra en la SEC ID NO: 1 ó 2; (B) polipéptido que tiene la misma secuencia de aminoácidos tal como se muestra en SEQ ID NO: 1 ó 2, exceptoque de 1 a 10 aminoácidos se han sustituido, eliminado, insertado y/o añadido, cuyo polipéptido tiene actividad de Dlactatodeshidrogenasa; y (C) polipéptido que tiene una secuencia de aminoácidos que tiene una identidad de secuencia de no menos del80% con respecto a la secuencia de aminoácidos que se muestra en la SEC ID NO: 1 ó 2, cuyo polipéptido tiene unaactividad de D-lactato deshidrogenasa.

公开(公告)号：CA2876114A1

公开(公告)日：2013-12-19

申请号：CA2876114

申请日：2013-06-11

Applicant: TORAY INDUSTRIES

Inventor： MINAMINO ATSUSHI ,  KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE

IPC: C12P19/14 ,  C12P1/00 ,  C12P7/10 ,  C12P7/56 ,  C13K1/04

Abstract: A method for producing a sugar solution by treating a cellulose-containing biomass with dilute sulfuric acid, said method comprising the below-mentioned steps (1) to (4). In the method, a higher treatment effect can be achieved compared with those achieved in the conventional treatment methods with dilute sulfuric acid. Step (1): a step of treating the cellulose-containing biomass with dilute sulfuric acid and then separating the resultant solution into a dilute sulfuric acid-treated solution and a cellulose-containing solid material. Step (2): a step of adding a cellulase to the cellulose-containing solid material to hydrolyze the cellulose-containing solid material, thereby producing a sugar solution. Step (3): a step of filtrating the dilute sulfuric acid-treated solution through a nano-filtration membrane at a pH of 2.5 or lower to separate out a sugar-rich solution as a non-filtrate and collect an aqueous sulfuric acid solution as a filtrate. Step (4): a step of reusing the whole or a portion of the aqueous sulfuric acid solution obtained in step (3) in the treatment with dilute sulfuric acid in step (1).

公开(公告)号：AU2012223954A1

公开(公告)日：2013-10-24

申请号：AU2012223954

申请日：2012-03-02

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMAMOTO YUKI ,  YAMADA KATSUSHIGE

IPC: C12P19/14 ,  C12P1/02 ,  C13K1/02

Abstract: The purpose of the present invention is to provide a method for producing a sugar solution, said method comprising the following steps (1)-(3). Step (1): a step in which a filamentous fungus-derived cellulase is added to a pretreated cellulose material so as to obtain a hydrolysis product Step (2): a step in which blackstrap molasses is added to and mixed with the hydrolysis product so as to obtain a mixed sugar solution Step (3): a step in which the mixed sugar solution is subjected to solid-liquid separation and the obtained solution component is filtered by being passed through an ultrafiltration membrane so as to recover the filamentous fungus-derived cellulase as a non-permeated liquid and to obtain a sugar solution as a permeated liquid The enzyme recovery ratio of the filamentous fungus-derived cellulase from the cellulose hydrolysis product is improved by the present invention, so that the amount of cellulase used in the production process of a sugar solution can be reduced. In addition, a sugar component can be recovered not only from cellulose but also from blackstrap molasses in the present invention by adding blackstrap molasses to a cellulose hydrolysis product, thereby obtaining a mixed sugar solution.

公开(公告)号：CA2864256A1

公开(公告)日：2013-08-22

申请号：CA2864256

申请日：2013-02-12

Applicant: TORAY INDUSTRIES

Inventor： NIWA MASAHIRO ,  SHINDO SHO ,  NISHIDA TAKANORI ,  KISHIMOTO JUNPEI ,  MINAMINO ATSUSHI ,  KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE

IPC: C13K1/02 ,  C12P7/06

Abstract: A method for producing a sugar solution according to the present invention is characterized by comprising: a pretreatment step (step S11) of treating a cellulose-type biomass with an ammonia-containing treatment agent to produce an ammonia-treated product; an ammonia-treated sugar solution production step (step S12) of enzymatically saccharifying the ammonia-treated product to produce an ammonia-treated sugar solution; and a purified sugar solution production step (step S13) of purifying and removing coumaramide and/or ferulamide contained in the ammonia-treated sugar solution to produce a purified sugar solution having a coumaramide and/or ferulamide concentration of 10 to 1100 ppm.

公开(公告)号：CA2856343A1

公开(公告)日：2013-05-30

申请号：CA2856343

申请日：2012-11-21

Applicant: TORAY INDUSTRIES

Inventor： KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE

IPC: C12N9/42 ,  B01D61/14 ,  B01D61/18 ,  B01D61/22 ,  B01D61/58 ,  B09B3/00 ,  C12M1/12

Abstract: A method for producing a cellulase, comprising steps (1) to (3) mentioned below, wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches, the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut-off molecular weight of 100,000 to 200,000 to produce a filtrate and a concentrated enzyme solution that is a non-filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut-off molecular weight of 5,000 to 50,000 to produce a second concentrated enzyme solution that is a non-filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus.

公开(公告)号：CA2848356A1

公开(公告)日：2013-03-21

申请号：CA2848356

申请日：2012-09-13

Applicant: TORAY INDUSTRIES

Inventor： MINAMINO ATSUSHI ,  KISHIMOTO JUNPEI ,  KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE

IPC: C12M1/00 ,  B01D61/02 ,  B01D61/14 ,  B01D61/58 ,  B09B3/00 ,  C12P19/14 ,  C13K1/02

Abstract: A sugar solution production apparatus (10A-1) comprises a saccharification tank (11) for obtaining a saccharified solution (16) including solid content from cellulose-containing biomass, a solid-liquid separation device (12) for separating out the solid content from the saccharified solution (16) to obtain a sugar solution (22), an enzyme recovery solution tank (13) for storing an enzyme recovery solution (24), a sugar solution extraction line (L11) for extracting the sugar solution (22) from the solid-liquid separation device (12), a hot water supplying means (13) for supplying hot water (25) to the solid-liquid separation device (12) to obtain the sugar solution (22) from the solid content with a saccharifying enzyme (15) adsorbed onto the solid content, an enzyme recovery solution supply line (L12) for supplying the enzyme recovery solution (24) to the solid-liquid separation device (12) from the enzyme recovery solution tank (13), an enzyme recovery solution recovery line (L13) for feeding to the enzyme recovery solution tank (13) the enzyme recovery solution (24) obtained by recovering the saccharifying enzyme (15) within the solid content, and an enzyme recovery solution return line (L14) for returning the enzyme recovery solution (24) to the saccharification tank (11).

公开(公告)号：CA2843384A1

公开(公告)日：2013-02-07

申请号：CA2843384

申请日：2012-07-27

Applicant: TORAY INDUSTRIES

Inventor： MINAMINO ATSUSHI ,  KURIHARA HIROYUKI ,  YAMADA KATSUSHIGE ,  KISHIMOTO JUNPEI

IPC: B01J20/24 ,  B01D25/12 ,  B01D36/00 ,  B01D37/02 ,  B01D61/02 ,  B01D61/14 ,  B01D61/58 ,  B01J20/30

Abstract: Disclosed is a cellulose-based filter aid with high turbidity removal capability, and a manufacturing method thereof. This method of manufacturing a filter aid involves (A) grinding and/or thermochemically treating a cellulose-containing biomass to obtain a pre-treated biomass, (B) a step for treating with cellulase the pre-treated biomass obtained in step (A) to obtain a cellulase-treated product, and a step (C) for obtaining the solid component of the cellulase-treated product of step (B). This cellulose-based filter aid contains the water-insoluble cellulase-treated product of a cellulose-containing biomass.