公开(公告)号：CA2620468A1

公开(公告)日：2007-02-22

申请号：CA2620468

申请日：2006-08-18

Applicant: BASF AG

Inventor： KROEMER JENS ,  SCHROEDER HARTWIG ,  WITTMANN CHRISTOPH ,  HEINZLE ELMAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  PERO JANICE ,  YOCUM ROGERS ,  WILLIAMS MARK ,  HERMAN THERON ,  PATTERSON THOMAS

IPC: C12N15/52 ,  C12P13/12

Abstract: The present invention relates to methods for the production of microorganisms with increased efficiency for methionine synthesis, microorganisms with increased efficiency for methionine synthesis, and methods for determining the optimal metabolic flux for organisms with respect to methionine synthesis.

公开(公告)号：ZA200404418B

公开(公告)日：2006-07-26

申请号：ZA200404418

申请日：2004-06-04

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABENHAUER GREGOR

IPC: C12N15/11 ,  C07K14/34 ,  C12N20060101 ,  C12N1/21 ,  C12N15/63 ,  C12P13/08

Abstract: Isolated nucleic acid (I) that encodes particular protein sequences (II) that have, at specific positions, an amino acid alteration. Independent claims are also included for: (1) Vector that contains at least one (I); (2) Host cell transfected with at least one vector of (1); and (3) Method for preparing fine chemicals by culturing cells of (2).

公开(公告)号：ZA200404423B

公开(公告)日：2006-05-31

申请号：ZA200404423

申请日：2004-06-04

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N20060101 ,  C12N1/21 ,  C12N9/00 ,  C12P13/08

Abstract: The invention relates to novel nucleic acid molecules, to the use thereof for constructing genetically improved microorganisms and to methods for preparing fine chemicals, in particular amino acids, with the aid of said genetically improved microorganisms.

公开(公告)号：AR047152A1

公开(公告)日：2006-01-11

申请号：ARP040104737

申请日：2004-12-17

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C12N9/02 ,  C12N15/77 ,  C12N15/31 ,  C12N15/67 ,  C12N15/63 ,  C12P13/08 ,  C12P13/12 ,  C12R1/15 ,  C12N1/21 ,  C12R1/13

Abstract: Se refiere al uso de secuencias de ácidos nucleicos para la regulacion de la transcripcion y la expresion de genes, a los nuevos promotores y unidades de expresion propiamente dichos, a procedimientos para la modificacion o activacion del grado de transcripcion y/o grado de expresion de genes, a casetes de expresion que contienen las unidades de expresion, a microorganismos modificados genéticamente con grado de transcripcion y/o grado de expresion modificado o activado, así como también a procedimientos para la preparacion de productos biosintéticos por medio del cultivo de los microorganismos genéticamente modificados. Reivindicacion 1: Uso de un ácido nucleico con actividad promotora, que contiene: A) la secuencia de ácidos nucleicos SEQ. ID. NO. 1 o B) una secuencia derivada de esta secuencia por sustitucion, insercion o delecion de nucleotidos, la que presenta una identidad de por lo menos 90 % a nivel de ácidos nucleicos con la secuencia SEQ. ID. NO. 1, o C) una secuencia de ácidos nucleicos, que hibrida con la secuencia de ácidos nucleicos SEQ. ID. NO. 1 bajo condiciones severas o D) fragmentos funcionalmente equivalentes de las secuencias de A), B) o C), para la transcripcion de genes.

公开(公告)号：AR047058A1

公开(公告)日：2006-01-04

申请号：ARP040104742

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12P20060101 ,  C12P13/08 ,  C12N15/54 ,  C12N1/21 ,  C12R1/15

Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, glicerol quinasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de glicerol quinasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado.

公开(公告)号：CA2546847A1

公开(公告)日：2005-12-22

申请号：CA2546847

申请日：2004-12-17

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  ZELDER OSKAR ,  WITTMANN CHRISTOPH ,  HAFNER STEFAN

IPC: C12P13/08 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12P20060101

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2004320598A1

公开(公告)日：2005-12-22

申请号：AU2004320598

申请日：2004-12-17

Applicant: BASF AG

Inventor： WITTMANN CHRISTOPH ,  HEINZLE ELMAR ,  SCHRODER HARTWIG ,  ZELDER OSKAR ,  KIEFER PATRICK ,  KROGER BURKHARD ,  HAFNER STEFAN ,  KLOPPROGGE CORINNA

IPC: C12P13/08 ,  C12P20060101

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：DE10359595A1

公开(公告)日：2005-07-28

申请号：DE10359595

申请日：2003-12-18

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N15/77 ,  C12P13/08 ,  C12N15/31 ,  C12N1/00

Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).

公开(公告)号：DE10359594A1

公开(公告)日：2005-07-28

申请号：DE10359594

申请日：2003-12-18

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/08 ,  C12P13/12

Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

公开(公告)号：CA2547792A1

公开(公告)日：2005-06-30

申请号：CA2547792

申请日：2004-12-16

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  KROEGER BURKHARD ,  ZELDER OSKAR ,  SCHROEDER HARTWIG ,  HABERHAUER GREGOR ,  RUFFER UWE ,  KLOPPROGGE CORINNA ,  GRAEF CLAUDIA ISABELLA

IPC: C07K14/34 ,  C12N5/10 ,  C12N9/90 ,  C12N15/31 ,  C12N15/63

Abstract: The invention relates to mutant nucleic acids and proteins from the metaboli c pathway of fine chemicals, methods for the production of genetically modifie d production organisms, methods for the production of fine chemicals by cultivating said genetically modified organisms, and said genetically modifi ed organisms.