公开(公告)号：KR1020110009402A

公开(公告)日：2011-01-28

申请号：KR1020090066787

申请日：2009-07-22

Applicant: 대한민국(농촌진흥청장)

Inventor： 한상익 ,  오기원 ,  하태정 ,  임시규 ,  고종민 ,  백인열 ,  박금룡 ,  남민희 ,  강항원 ,  안진곤

IPC: A01G7/00 ,  A01G7/04 ,  A23L11/00

CPC classification number: A01G7/00 ,  A01G7/04 ,  A01G7/045 ,  A23L11/00

Abstract: PURPOSE: An isoflavone content increasing method inside a bean seed is provided to use a lighting process to beans during the vegetative period of the beans. CONSTITUTION: An isoflavone content increasing method inside a bean seed includes a step of light-processing beans during the vegetative period of the beans. The intensity of the lighting process is 20~50lux. The lighting process is performed for 10~30days, 1~4hours after the sunrise and 1~3hours after the sunset. The beans are glycine max.

Abstract translation: 目的：在豆类种植期间提供异黄酮含量增加方法，以在豆类营养期内对豆类进行照明处理。 构成：豆类种子中异黄酮含量的增加方法包括在豆类营养期间轻度加工豆类的步骤。 照明过程的强度为20〜50lux。 照明过程进行10〜30天，日出后1〜4小时，日落后1〜3小时。 豆类最多为甘氨酸。

公开(公告)号：KR1020100095981A

公开(公告)日：2010-09-01

申请号：KR1020090015067

申请日：2009-02-23

Applicant: 대한민국(농촌진흥청장)

Inventor： 박동수 ,  이기환 ,  한상익 ,  남민희 ,  송송이 ,  왕회정 ,  박수권 ,  이봉춘 ,  송유천 ,  강항원

IPC: C12N15/29 ,  A01H1/00

Abstract: PURPOSE: A rice plant with transformed giant embryonic rice is provided to ensure a heavier product than conventional embryos and to obtain rice containing zinc and iron. CONSTITUTION: A cytochrome P450 of giant embryonic rice comprises a base sequence which encodes an amino acid sequence of cytochrome P450 and encodes leucine to a 395th amino acid after translation. A cytochrome P450 gene of rice is a cDNA encoding cytochrome P450. A rice plant is produced by transforming rice plant with a recombinant vector having cytochrome P450 gene. The giant embryonic rice is YR23517Acp79.

Abstract translation: 目的：提供具有转化的巨型胚胎稻的水稻，以确保比常规胚胎更重的产品，并获得含锌和铁的水稻。 构成：巨型胚胎水稻的细胞色素P450包含编码细胞色素P450氨基酸序列的碱基序列，并在翻译后将亮氨酸编码为第395位氨基酸。 水稻的细胞色素P450基因是编码细胞色素P450的cDNA。 通过用具有细胞色素P450基因的重组载体转化水稻植物来生产水稻。 巨型胚胎大米是YR23517Acp79。

公开(公告)号：KR1020100079527A

公开(公告)日：2010-07-08

申请号：KR1020080138049

申请日：2008-12-31

Applicant: 대한민국(농촌진흥청장)

Inventor： 한상익 ,  서득용 ,  이명희 ,  하태정 ,  정찬식 ,  박금룡 ,  안진곤

IPC: C12N15/10 ,  C12Q1/68 ,  G01N33/68

CPC classification number: C12N15/11 ,  C12Q1/6888 ,  C12Q2600/156

Abstract: PURPOSE: An SSR primer isolated from Vigna angularis (Willd.) Ohwi and Ohashi and a method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi using the same are provided to effectively detect DNA polymorphism and to determine the product species of Vigna angularis (Willd.) Ohwi and Ohashi. CONSTITUTION: An SSR(simple sequence repeat) primer pair comprises two base sequences selected from sequence numbers 1-12. The SSR primer pair is derived from Vigna angularis (Willd.) Ohwi and Ohashi. A method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi comprises: a step of extracting genome DNA from Vigna angularis (Willd.) Ohwi and Ohashi; a step of performing PCR using SSR primer; and a step of performing electrophoresis.

Abstract translation: 目的：从Vigna angularis（Willd。）Ohwi和Ohashi分离的SSR引物和用于检测Vigna angularis（Willd。）Ohwi和Ohashi的DNA多态性的方法，用于有效检测DNA多态性并确定产物种类 Vigna angularis（Willd。）Ohwi和Ohashi。 构成：SSR（简单序列重复）引物对包含从序列号1-12中选择的两个碱基序列。 SSR引物对衍生自Vigna angularis（Willd。）Ohwi和Ohashi。 用于检测Vigna angularis（Willd。）Ohwi和Ohashi的DNA多态性的方法包括：从Vigna angularis（Willd。）Ohwi和Ohashi提取基因组DNA的步骤; 使用SSR引物进行PCR的步骤; 和进行电泳的步骤。

公开(公告)号：KR1020010017748A

公开(公告)日：2001-03-05

申请号：KR1019990033413

申请日：1999-08-13

Applicant: 대한민국(농촌진흥청장)

Inventor： 김금숙 ,  박창기 ,  성재덕 ,  김현태 ,  한상익 ,  곽용호 ,  임무상

IPC: C07H15/20 ,  C07H1/08

Abstract: PURPOSE: Provided is a natural aspaoligonin isolated from Asaragus oligoclonos Maxim. Also, an isolating method thereof is provided. The aspaoligonin is potentially developed into medical supplies if its medicinal action is examined. CONSTITUTION: The aspaoligonin represented by formula (1) is isolated from Asaragus oligoclonos Maxim. by the following steps of: grinding the dried roots of Asaragus oligoclonos Maxim.; extracting the grinded roots with methanol at room temperature; filtering the extract solution and concentrating the filtrate under vacuum condition; dissolving the concentrates in distilled water and defatting the solution with ether; extracting the water layer of the defatted solution with butanol to obtain the butanol fragment; purifying the butanol fragment by chromatography on silica gel to obtain 20 fragments; and purifying the 5th fragment by using Sep-Pak C18 to obtain aspaoligonin.

Abstract translation: 目的：提供从Asaragus oligoclonos Maxim分离的天然天冬白。素。 此外，还提供了其隔离方法。 如果检查其药物作用，则天冬氨酸可能发展成为医疗用品。 构成：式（1）所代表的天冬白蛋白从蛔虫（Asaragus oligoclonos Maxim）中分离出来。 通过以下步骤：研磨Asaragus oligoclonos Maxim的干燥根; 在室温下用甲醇萃取研磨的根; 过滤提取液并在真空条件下浓缩滤液; 将浓缩物溶解在蒸馏水中并用乙醚脱溶液; 用丁醇提取脱脂溶液的水层，得到丁醇片; 通过硅胶色谱纯化丁醇片段得到20个片段; 并用Sep-Pak C18纯化第5片，得到天冬氨酸。