公开(公告)号：WO2006113727A3

公开(公告)日：2007-02-15

申请号：PCT/US2006014583

申请日：2006-04-19

Applicant: HARVARD COLLEGE ,  LINDER VINCENT ,  SIA SAMUEL K ,  WHITESIDES GEORGE M ,  NAROVLYANSKY MAX ,  SIEGEL ADAM

Inventor： LINDER VINCENT ,  SIA SAMUEL K ,  WHITESIDES GEORGE M ,  NAROVLYANSKY MAX ,  SIEGEL ADAM

IPC: G01N21/59 ,  B01L3/00 ,  G01N33/543 ,  G01N33/553

CPC classification number: G01N33/54366 ,  B01L3/502746 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0883 ,  B01L2400/0406 ,  G01N21/01 ,  G01N21/82 ,  G01N33/54373

Abstract: The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analyses due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area. Although this invention mainly describes the use of meandering and wide channels in heterogeneous immunoassays on a microfluidic chip, this invention could be used for amplifying optical signals for other types of reactions and/or assays.

Abstract translation: 本发明一般涉及微流体结构，更具体地涉及包括曲折和宽通道的微流体结构和方法。 微流控系统可以提供用于执行各种反应和分析的有利环境，这是由于与常规系统相比所需的样品和试剂量的减少，操作系统尺寸的减小以及反应时间的减少。 不幸的是，微量流体通道的小尺寸有时会导致在不放大光学器件（例如显微镜或光电倍增管）的情况下检测物质的困难。 一系列紧密包装的微通道，即曲折区域或具有大约毫米数量级的宽通道可以通过创建宽的测量区域来解决这个问题。 尽管本发明主要描述了在微流体芯片上的异源免疫测定中使用曲折和宽通道，但是本发明可用于放大用于其他类型反应和/或测定的光学信号。

公开(公告)号：WO2004002627A8

公开(公告)日：2005-03-17

申请号：PCT/US0320542

申请日：2003-06-30

Applicant: HARVARD COLLEGE ,  STONE HOWARD A ,  ANNA SHELLY L ,  BONTOUX NATHALIE ,  LINK DARREN ROY ,  WEITZ DAVID A ,  GITLIN IRINA ,  GARSTECKI PIOTR ,  DILUZIO WILLOW ,  WHITESIDES GEORGE M ,  KUMACHEVA EUGENIA

Inventor： STONE HOWARD A ,  ANNA SHELLY L ,  BONTOUX NATHALIE ,  LINK DARREN ROY ,  WEITZ DAVID A ,  GITLIN IRINA ,  GARSTECKI PIOTR ,  DILUZIO WILLOW ,  WHITESIDES GEORGE M ,  KUMACHEVA EUGENIA

IPC: B01F3/08 ,  B01F5/06 ,  B01F13/00 ,  B01L3/00 ,  B05B7/04

CPC classification number: B01L3/5027 ,  B01F3/0807 ,  B01F5/0682 ,  B01F5/0688 ,  B01F13/0062 ,  B01F2215/0431 ,  B01F2215/045 ,  B05B7/0408 ,  B05B7/0416 ,  B05B7/0441 ,  Y10S516/924 ,  Y10S516/927 ,  Y10T29/49002 ,  Y10T137/0324 ,  Y10T137/0329 ,  Y10T137/206 ,  Y10T137/87346 ,  Y10T436/2575

Abstract: A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.

Abstract translation: 提供了用于在流体中聚焦和/或形成类似或不同尺寸的不连续部分的微流体方法和装置。 该设备可以简单地使用简单的技术从容易获得的廉价材料制造。

公开(公告)号：WO0222264A8

公开(公告)日：2002-07-18

申请号：PCT/US0142195

申请日：2001-09-18

Applicant: HARVARD COLLEGE ,  JEON NOO LI ,  DERTINGER STEPHAN K W ,  CHIU DANIEL T ,  CHOI INSUNG S ,  WHITESIDES GEORGE M

Inventor： JEON NOO LI ,  DERTINGER STEPHAN K W ,  CHIU DANIEL T ,  CHOI INSUNG S ,  WHITESIDES GEORGE M

IPC: G01N33/53 ,  B01D3/00 ,  B01F5/06 ,  B01F13/00 ,  B01F15/04 ,  B01J4/00 ,  B01J19/00 ,  B01J19/24 ,  B01L20060101 ,  B01L1/00 ,  B01L3/00 ,  B65B3/00 ,  B65B3/04 ,  C12M1/34 ,  C23G1/00 ,  G01F11/28 ,  G01N1/28 ,  G01N1/38 ,  G01N33/543 ,  G01N37/00 ,  B01F4

CPC classification number: B01F5/0601 ,  B01F5/064 ,  B01F13/0059 ,  B01F15/0404 ,  B01J4/001 ,  B01J19/0093 ,  B01J2219/0086 ,  B01J2219/00889 ,  B01L3/5027 ,  G01N1/2813 ,  G01N1/38

公开(公告)号：WO0222787A3

公开(公告)日：2002-07-11

申请号：PCT/US0129248

申请日：2001-09-18

Applicant: HARVARD COLLEGE ,  TAKAYAMA SHUICHI ,  OSTUNI EMANUELE ,  LEDUC PHILIP ,  NARUSE KEIJI ,  INGBER DONALD E ,  WHITESIDES GEORGE M

Inventor： TAKAYAMA SHUICHI ,  OSTUNI EMANUELE ,  LEDUC PHILIP ,  NARUSE KEIJI ,  INGBER DONALD E ,  WHITESIDES GEORGE M

IPC: G01N37/00 ,  A61K35/12 ,  B81B1/00 ,  C12N5/00 ,  C12N5/071 ,  G01N33/50 ,  G01N33/52

CPC classification number: G01N33/5011 ,  A61K35/12 ,  C12N5/00 ,  C12N5/069 ,  C12N2501/48 ,  C12N2501/58 ,  C12N2503/00 ,  C12N2503/02 ,  C12N2510/00 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5035 ,  G01N33/5076 ,  G01N33/5079 ,  G01N33/528 ,  G01N2800/52

Abstract: The present invention is directed, in certain embodiments, to improved, small scale systems and methods able to selectively treat parts of a single cell, including, in certain embodiments, portions of a main body portion of a single cell, and able, in certain embodiments, to establish long-term gradients of active substances within subcellular regions of a single cell. The present invention provides, in some embodiments, techniques for selectively contacting a portion of the surface of a biological cell with a fluid or fluid component carrying a particular potential for a physiological or biochemical interaction with the cell, and simultaneously contacting a different portion of the surface of the cell with another fluid or fluid component having a different portion of the surface of the cell with another fluid or fluid component having a different potential for the biophysical or biochemical interaction with the cell.

Abstract translation: 在某些实施方案中，本发明涉及能够选择性处理单个细胞的部分的改进的小规模系统和方法，在某些实施方案中包括单个细胞的主体部分的一部分，并且能够在某些 实施例，以建立单细胞的亚细胞区域内的活性物质的长期梯度。 在一些实施方案中，本发明提供了用于选择性接触生物细胞表面的一部分与携带与细胞的生理或生物化学相互作用的特定可能性的流体或流体组分并且同时接触细胞的不同部分 细胞表面与具有细胞表面的不同部分的另一种流体或流体成分与具有与细胞的生物物理或生物化学相互作用的不同电势的另一种流体或流体成分。

公开(公告)号：WO0151276A3

公开(公告)日：2002-01-03

申请号：PCT/US0100366

申请日：2001-01-05

Applicant: HARVARD COLLEGE

Inventor： DENG TAO ,  ARIAS FRANCISCO ,  ISMAGILOV RUSTEM F ,  KENIS PAUL J A ,  WHITESIDES GEORGE M

IPC: B81C1/00 ,  C23C18/16 ,  H05K3/10 ,  H05K3/18 ,  C25D5/54 ,  H01L21/768 ,  H05K3/00

CPC classification number: B81C1/00095 ,  B81B2207/07 ,  B81C99/008 ,  B81C99/0095 ,  B82Y30/00 ,  C23C18/06 ,  C23C18/14 ,  C23C18/1603 ,  C23C18/1608 ,  C23C18/1612 ,  C23C18/1653 ,  C23C18/1657 ,  C23C18/208 ,  C23C18/36 ,  C23C18/42 ,  H05K3/105 ,  H05K3/106 ,  H05K3/185 ,  H05K3/202 ,  H05K3/205 ,  H05K2203/0117 ,  H05K2203/0156 ,  H05K2203/302 ,  Y10T29/49986

Abstract: A method of forming microstructures. An article (110) including a metal atom precursor (120) is disproportionally exposed to electromagnetic radiation in an amount and intensity sufficient to convert some of the precursor to elemental metal (160). Additional conductive material may then be deposited onto the elemental metal to produce a microstructure.

Abstract translation: 一种形成微观结构的方法。 包含金属原子前体（120）的物品（110）不适当地暴露于电磁辐射，其量和强度足以将一些前体转化为元素金属（160）。 然后可以将另外的导电材料沉积到元素金属上以产生微结构。

公开(公告)号：WO2017014828A3

公开(公告)日：2017-03-16

申请号：PCT/US2016032706

申请日：2016-05-16

Applicant: HARVARD COLLEGE ,  UNIV SOUTHAMPTON

Inventor： TSALOGLOU MARIA-NEFELI ,  NEMIROSKI ALEX ,  CHRISTODULEAS DIONYSIOS ,  CAMCI-UNAL GULDEN ,  CONNELLY JOHN T ,  FERNANDEZ-ABEDUL MARIA TERESA ,  WHITESIDES GEORGE M

IPC: G01N27/26 ,  G01N27/327 ,  G01N27/416

CPC classification number: C12Q1/68 ,  B01L3/5023 ,  B01L3/5027 ,  B01L7/00 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2300/1827 ,  G01N33/543

Abstract: A microfluidic, electrochemical device for detecting a genetic material is described, including: one or more cellulosic layers comprising at least one of a hydrophilic test zone and a hydrophilic sample deposition zone in fluid communication with each other; one or more amplification agents selected for amplifying a genetic material and embedded in the hydrophilic test zone or the sample deposition zone; one or more binding agents embedded in the test zone or the sample deposition zone and selected for binding the amplified genetic material to result in a change of the concentration of a signaling chemical, wherein the signaling chemical is either embedded in the test zone or the sample deposition zone prior to the binding or is a newly generated product of the binding; and an electrode assembly comprising one or more electrodes in fluidic contact with the test zone and configured to interact with the signaling chemical to result in a current change readable by an electrochemical reader.

Abstract translation: 描述了一种用于检测遗传物质的微流体电化学装置，包括：一个或多个纤维素层，其包含彼此流体连通的亲水测试区和亲水样品沉积区中的至少一个; 选择用于扩增遗传物质并包埋在亲水测试区或样品沉积区中的一种或多种扩增剂; 嵌入测试区或样品沉积区中并选择用于结合扩增的遗传物质导致信号化学物质浓度变化的一种或多种结合剂，其中所述信号传导化学物质嵌入测试区或样品 在结合之前的沉积区或者是新产生的结合产物; 以及电极组件，其包括与所述测试区流体接触的一个或多个电极，并且被配置为与所述信号化学物质相互作用以导致电化学读数器可读的电流变化。

公开(公告)号：WO2015105556A3

公开(公告)日：2015-11-05

申请号：PCT/US2014061387

申请日：2014-10-20

Applicant: HARVARD COLLEGE

Inventor： SHEPHERD ROBERT F ,  WHITESIDES GEORGE M ,  MOSADEGH BOBAK

IPC: A61B1/32

CPC classification number: A61B17/0218 ,  A61B1/00082 ,  A61B1/3132 ,  A61B1/32 ,  A61B2017/00557 ,  A61B2017/00831 ,  A61B2017/0225

Abstract: A surgical device for displacement of organs within a body cavity for providing at least visual access to a selected site includes an expandable bladder, wherein the elasticity of the bladder varies across the surface of the bladder, said variation in elasticity selected to provide a predetermined, non-spherical shape when expanded; and a valve on the proximal end on the inflatable bladder for introduction of a pressurizing gas into the soft bladder.

Abstract translation: 用于移动体腔内器官的外科手术装置，用于至少提供对选定部位的视觉进入，包括可膨胀囊，其中，膀胱的弹性在膀胱的表面上变化，所述弹性变化被选择为提供预定的， 膨胀时非球形; 以及位于可膨胀气囊上的近端上的阀，用于将加压气体引入软气囊中。

公开(公告)号：WO2014176595A2

公开(公告)日：2014-10-30

申请号：PCT/US2014035697

申请日：2014-04-28

Applicant: HARVARD COLLEGE ,  CHILDRENS MEDICAL CENTER

Inventor： WHITESIDES GEORGE M ,  KUMAR ASHOK A ,  HENNEK JONATHAN W ,  LIM CAEUL ,  MORENO YOVANY ,  MACE CHARLES R ,  DURAISINGH MANOJ T ,  PATTON MATTHEW R ,  LEE RYAN ,  D ALESIO-SPINA GAETANA ,  BRUGNARA CARLO

IPC: C12Q1/00

CPC classification number: G01N33/80 ,  G01N15/05 ,  G01N33/491 ,  G01N2015/003 ,  G01N2015/0073 ,  G01N2015/0076 ,  G01N2800/22

Abstract: Multi-phase systems and kits using these multiphase systems are described. The Multi-phase system described herein comprises multiple phase-separated phases each comprises a phase component and the phases, taken together, represent a density gradient. The kit comprising the multiphase system as described herein may be used to separate biological analytes such as cells. Non- limiting examples of the biological analytes include normal erythrocyte with hemoglobin Hb AA, Hb CC, and Hb AS, sickle cell erythrocyte with hemoglobin Hb SS and Hb SC, reticulocyte, iron deficiency anemia red blood cell, &bgr;-thalessemia trait red blood cell, and normal red blood cell.

Abstract translation: 描述了使用这些多相系统的多相系统和套件。 本文描述的多相系统包括多个相分离相，每个相包含相分量，并且相合在一起表示密度梯度。 包含如本文所述的多相系统的试剂盒可用于分离生物分析物如细胞。 生物分析物的非限制性实例包括具有血红蛋白Hb AA，Hb CC和Hb AS的正常红细胞，具有血红蛋白Hb SS和Hb SC的镰状红细胞，网织红细胞，缺铁性贫血红血细胞，β-贫血性状红血细胞 细胞和正常的红细胞。

公开(公告)号：WO2012148472A3

公开(公告)日：2013-07-25

申请号：PCT/US2011061720

申请日：2011-11-21

Applicant: HARVARD COLLEGE ,  ILIEVSKI FILIP ,  CHEN XIN ,  MAZZEO AARON D ,  WHITESIDES GEORGE M ,  SHEPHERD ROBERT ,  MARTINEZ RAMSES ,  CHOI WON JAE ,  KWOK SEN W ,  MORIN STEPHEN ,  STOKES ADAM ,  NIE ZHIHONG

Inventor： ILIEVSKI FILIP ,  CHEN XIN ,  MAZZEO AARON D ,  WHITESIDES GEORGE M ,  SHEPHERD ROBERT ,  MARTINEZ RAMSES ,  CHOI WON JAE ,  KWOK SEN W ,  MORIN STEPHEN ,  STOKES ADAM ,  NIE ZHIHONG

IPC: B25J9/00

CPC classification number: F15B15/10 ,  B25J9/1075 ,  B25J9/142 ,  B25J11/00 ,  F15B7/06

Abstract: A soft robotic device includes a flexible body having a width, a length and a thickness, wherein the thickness is at least 1 mm, the flexible body having at least one channel disposed within the flexible body, the channel defined by upper, lower and side walls, wherein at least one wall is strain limiting; and a pressurizing inlet in fluid communication with the at least one channel, the at least one channel positioned and arranged such that the wall opposite the strain limiting wall preferentially expands when the soft robotic device is pressurized through the inlet.

Abstract translation: 软机器人装置包括具有宽度，长度和厚度的柔性体，其中所述厚度为至少1mm，所述柔性体具有设置在所述柔性体内的至少一个通道，所述通道由上，下和侧限定 壁，其中至少一个壁是应变限制; 以及与所述至少一个通道流体连通的加压入口，所述至少一个通道定位和布置成使得当所述软自动装置通过所述入口被加压时，与所述应变限制壁相对的壁优先地膨胀。

公开(公告)号：WO2013052171A3

公开(公告)日：2013-06-20

申请号：PCT/US2012040171

申请日：2012-05-31

Applicant: HARVARD COLLEGE ,  CADEMARTIRI LUDOVICO ,  MACE CHARLES R ,  SHEPHERD ROBERT FOSTER ,  MAZZEO AARON D ,  BISHOP KYLE J M ,  CHIECHI RYAN C ,  WHITESIDES GEORGE M

Inventor： CADEMARTIRI LUDOVICO ,  MACE CHARLES R ,  SHEPHERD ROBERT FOSTER ,  MAZZEO AARON D ,  BISHOP KYLE J M ,  CHIECHI RYAN C ,  WHITESIDES GEORGE M

IPC: F23D14/00 ,  F23D14/84 ,  F23D99/00 ,  F23N5/00

CPC classification number: F23N5/00 ,  F23C99/001

Abstract: Manipulation of flames is described using electric fields. In those instances in which electric fields are used, the electric fields may be time-varying gradient electric fields, and in some instances may be oscillating electiic fields. The manipulation may include extinction, suppression, control of mixing of the flame, concentration, and/or bending, among other types.

Abstract translation: 使用电场描述火焰的操纵。 在使用电场的情况下，电场可以是时变梯度电场，并且在一些情况下可以是振荡电场。 操作可以包括灭火，抑制，火焰混合的控制，浓缩和/或弯曲等。