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公开(公告)号：KR1020200145895A
公开(公告)日：2020-12-31
申请号：KR1020190073039
申请日：2019-06-19
Applicant: 대한민국(농촌진흥청장) , 명지대학교 산학협력단
IPC: C12Q1/6895
Abstract: 본발명의쑥 품종판별용 SSR(Simple Sequence Repeat) 프라이머세트, 상기프라이머세트를포함하는쑥 품종판별용키트및 이를이용한쑥 품종판별방법에관한것으로, 쑥의유전적인식별을통한품질규격과유통질서의확립을위한체계적인품종관리에활용가능하다.
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公开(公告)号：KR1020150058593A
公开(公告)日：2015-05-29
申请号：KR1020130139794
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은고도불포화지방산생합성에관한것으로, 보다상세하게는 pAO815 벡터를이용한세 개의유전자와및가동시에발현되도록다중발현벡터를만들어식물체에형질전환하고, 상기식물체의종자에서오메가-3 및오메가-6 고도불포화지방산인 GLA, STA, DGLA, ETA, ARA, EPA, DTA 및 DPA가종자에서생합성되는것을확인하였으므로, 이를고도불포화지방산생산자원으로유용하게사용할수 있다.
Abstract translation: 本发明涉及多不饱和脂肪酸的生物合成。 更具体地，本发明制备多表达载体以使植物同时使用pAO815载体转化表达McD6DES，AsELOVL5和PtD5DES基因。 本发明证实，在植物种子中生物合成了ω-3和ω-6的多不饱和脂肪酸，如GLA，STA，DGLA，ETA，ARA，EPA，DTA和DPA。 因此，本发明可有效地用作多不饱和脂肪酸生产资源。
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公开(公告)号：KR1020110063920A
公开(公告)日：2011-06-15
申请号：KR1020090120292
申请日：2009-12-07
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/93 , C12N15/81 , C12N15/8247 , C12P7/6427 , C12Y602/01003
Abstract: PURPOSE: A fatty acid elongase protein derived from Sparus aurata is provided to develop crop which produces polyunsaturated fatty acid(EPA, DHA). CONSTITUTION: A Sparus aurata-derived fatty acid elongase contains an amino acid of sequence number 2. A gene encoding the fatty acid elongase contains a base sequence of sequence number 1. pYES2-SaFAE vector contains the gene encoding the fatty acid elongase. A host cell transformed by a recombinant vector is yeast. A method for preparing functional polyunsaturated fatty acid in a host cell comprises a step of transforming the host cell with the recombinant vector. The polyunsaturated fatty acid is gamma linolenic acid(GLA), eicosapentaenoic acid(EPA), or docosa hexaenoic acid(DHA).
Abstract translation: 目的：提供衍生自Sparus aurata的脂肪酸延伸酶蛋白，以开发产生多不饱和脂肪酸（EPA，DHA）的作物。 构成：来自天竺葵的脂肪酸延长酶含有序列号2的氨基酸。编码脂肪酸延伸酶的基因含有序列号1的碱基序列。pYES2-SaFAE载体含有编码脂肪酸延伸酶的基因。 由重组载体转化的宿主细胞是酵母。 在宿主细胞中制备功能性多不饱和脂肪酸的方法包括用重组载体转化宿主细胞的步骤。 多不饱和脂肪酸是γ-亚麻酸（GLA），二十碳五烯酸（EPA）或二十二碳六烯酸（DHA）。
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15.发明授权안토시아닌 생합성 억제 활성을 가진 ＭＹＢ６０ 유전자와상기 유전자가 도입된 형질전환 상추 및 그 제조방법 有权用于抑制花青素生物合成的MYB60基因，MYB60基因转导的转基因莴苣及其制备方法
公开(公告)号：KR100809736B1
公开(公告)日：2008-03-05
申请号：KR1020060043300
申请日：2006-05-15
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 애기장대 유래의 전사조절 유전자 MYB60 및 상기 유전자를 도입한 형질전환 상추에 관한 것이다. 보다 상세하게는 안토시아닌 생합성 억제 활성을 가진 전사조절 유전자 MYB60과 상기 유전자를 도입한 형질전환 상추 및 상기 형질전환 상추를 제조하는 방법에 관한 것으로서, 상기 본 발명을 통하여 화훼작물을 비롯한 화색 조절을 목적으로 하는 작물에 도입, 응용함으로써 향후 농가의 부가가치를 높이는데 기여할 수 있다.
안토시아닌, 전사조절인자, 아그로박테리움, 상추, 애기장대, 형질전환, bar.Abstract translation: 提供了一种MYB60基因来抑制花青素的生物合成，因此可用于改变花卉植物的花色和开发新品种的花卉植物。 还提供了含有相同MYB60基因的转基因莴苣，以通过使用植物系统来验证基因通过表型的花青素生物合成抑制作用。 抑制花青素生物合成的MYB60基因具有SEQ ID NO：1的核苷酸序列。 载体22103-MYB60含有MYB60基因。 用载体22103-MYB60生产转化的根癌土壤杆菌EHA105（KACC 95045P）。 一种制备转基因莴苣的方法包括以下步骤：（a）制备含有MYB60基因和巴斯坦耐药基因作为选择标记的表达载体; （b）通过用表达载体转化产生根癌土壤杆菌EHA105（KACC 95045P）; （c）用转化的根癌土壤杆菌EHA105（KACC 95045P）共同培养莴苣植物的子叶; （d）从选择培养基中选择莴苣植物芽，并诱导植物诱导培养基中的芽; 和（e）将诱导的莴苣植物移植到体外培养基和土壤以适应它们。
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Patent Agency Ranking
公开(公告)号：KR100794296B1
公开(公告)日：2008-01-14
申请号：KR1020070003113
申请日：2007-01-11
Applicant: 대한민국(농촌진흥청장)
Abstract: An RNAi carrier is provided to be used for obtaining a transgenic Perilla frutescens which has the 4% decreased alpha-linolenic acid content and extended storage period and decreases the rancidity problem and foul odor generated during the rancidity. An RNAi carrier for inhibiting the expression of delta 15 desaturase is described in SEQ ID : NO. 2. A Perilla frutescens is transformed by the RNAi carrier and is characterized in that it has reduced alpha-linolenic acid content and improved herbicide resistance.
Abstract translation: 提供RNAi载体用于获得具有4％降低的α-亚麻酸含量和延长储存期的转基因紫苏，减少酸败问题和酸败期间产生的恶臭。 用于抑制δ15去饱和酶表达的RNAi载体描述于SEQ ID NO： 紫苏子被RNAi载体转化，其特征在于它具有降低的α-亚麻酸含量和改善的除草剂抗性。
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公开(公告)号：KR100771435B1
公开(公告)日：2007-10-31
申请号：KR1020060111108
申请日：2006-11-10
Applicant: 대한민국(농촌진흥청장)
IPC: C07K14/415
Abstract: A flavonol bio-synthesizing protein and a gene encoding the same are provided to synthesize flavonol, a secondary metabolic product of a plant, thereby being usefully used for developing a novel high-value crop and being used as a genetical engineering materials of the plant. A flavonol bio-synthesizing protein includes an amino acid sequence described as SEQ ID : NO. 1. A polynucleotide codes the protein and is characterized in that it has a sequence described as SEQ ID : NO. 1. A method for producing the flavonol bio-synthesizing protein comprises a step of culturing a transformed bacteria including a recombinant vector of pET-CsFLS. A method for bio-synthesizing flvaonol comprises a step of reacting the protein in a solution including a substrate such as dihydroquercetin, dihydrokaempferol and dihydromyricetin at a temperature of 25-35 deg.C for 40-80 minutes.
Abstract translation: 提供黄酮醇生物合成蛋白及其编码基因，以合成植物的次生代谢产物黄酮醇，从而有效地用于开发新型高价值作物，并用作植物的遗传工程材料。 黄酮醇生物合成蛋白包括SEQ ID NO：1所示的氨基酸序列。 1.一种多核苷酸编码该蛋白质，其特征在于其具有如SEQ ID NO： 1.生产黄酮醇生物合成蛋白的方法包括培养包含pET-CsFLS重组载体的转化细菌的步骤。 生物合成flvaonol的方法包括在25-35℃的温度下将蛋白质在包括底物如二氢槲皮素，二氢鹤丙酮和二氢糠氨酸的溶液中反应40-80分钟的步骤。
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公开(公告)号：KR100685520B1
公开(公告)日：2007-03-09
申请号：KR1020060000783
申请日：2006-01-04
Applicant: 대한민국(농촌진흥청장)
Abstract: A seed specific promoter having a novel nucleotide sequence, a primer for amplifying the promoter, an expression vector including the promoter and a plant cell transformed by the expression vector are provided to be able to limit the expression on seeds without influencing on the plant growth by seed-specifically inducing a target protein gene. The seed specific promoter consists of SEQ ID : NO. 1. The primer for amplifying seed specific promoter consists of an oligonucleotide consisting of SEQ ID : NO. 2 and an oligonucleotide consisting of SEQ ID : NO. 3. The expression vector pBGWFS7-PfOle20 deposited as a deposition number of KACC 95044P includes a promoter sequence described as SEQ ID : NO. 1. The plant cell is transformed by the expression vector pBGWFS7-PfOle20.
Abstract translation: 提供具有新核苷酸序列的种子特异性启动子，用于扩增启动子的引物，包含启动子的表达载体和由表达载体转化的植物细胞，以能够限制在种子上的表达而不影响植物生长， 种子特异性诱导靶蛋白基因。 种子特异性启动子由SEQ ID NO： 用于扩增种子特异性启动子的引物由SEQ ID NO：1组成的寡核苷酸组成。 2和由SEQ ID NO：2组成的寡核苷酸。 作为KACC 95044P的保藏号保藏的表达载体pBGWFS7-PfOle20包含如SEQ ID NO：1所述的启动子序列。 1.植物细胞被表达载体pBGWFS7-PfOle20转化。
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19.发明授权녹차 어린잎 유래 델타-6 불포화효소 유전자, 이 유전자를포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체및 이 형질전환체의 제조방법 有权来自玉米螟的DELTA-6去饱和基因，包含基因的表达载体，转化体转化的转化体和转化体的生产方法
公开(公告)号：KR100606612B1
公开(公告)日：2006-07-31
申请号：KR1020040117577
申请日：2004-12-31
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 녹차 어린잎 유래 델타-6 불포화효소 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이 형질전환체의 제조방법에 관한 것으로서, 더욱 상세하게는, 서열번호 1로 기재되는 염기서열로 이루어지는, 녹차 어린잎 유래 델타-6 불포화효소를 코딩하는 유전자, 이 유전자를 포함하는 발현벡터 pYES2/CT-D6DES, 이 발현벡터로 형질전환된 효모(
Saccharomyces cerevisiae ) 형질전환체(수탁번호: KACC 93021) 및 이 형질전환체의 제조방법에 관한 것이다.
델타-6 불포화효소, pYES2/CT, Saccharomyces cerevisiae-
公开(公告)号：KR100293567B1
公开(公告)日：2001-09-17
申请号：KR1019980009096
申请日：1998-03-17
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A tomato having cucumber mosaic virus tolerance is provided, thereby effectively inhibiting a plant from the infection with the cucumber mosaic virus. CONSTITUTION: The method for producing a tomato having the cucumber mosaic virus tolerance comprises the steps of: sequencing a surface protein gene of a cucumber mosaic virus isolated from Gladiolus having the nucleotide sequence of figure 1; preparing an expression vector containing the surface protein gene of cucumber mosaic virus isolated from Gladiolus; transforming Agrobacterium tumefaciens LBA4404 with the expression vector; transfecting a tomato with the transformed Agrobacterium tumefaciens LBA4404; and testing the cucumber mosaic virus tolerance by inoculating the cucumber mosaic virus into the transfected tomato.
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