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公开(公告)号：KR1020090112492A
公开(公告)日：2009-10-28
申请号：KR1020080038415
申请日：2008-04-24
Applicant: 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
Abstract: PURPOSE: A bidirectional promoter of sweet potato leaf curl infection DNA virus is provided to produce vector product in molecular biology field and molecular breeding industry. CONSTITUTION: A bidirectional promoter of sweet potato leaf curl infection DNA virus is denoted by the sequence of the sequence number 1. A method for identifying the promoter activity comprises: a step of isolating a site with promoter activity and analyzing cis-acting regulation site; a step of recombining promoter site by using gateway cloning to a plant expression vector for promoter analysis; a step of transforming the recombined promoter site to Agrobacteria tumefaciens strain GV3101; and a step of screening transformant.
Abstract translation: 目的：提供甘薯叶卷曲感染DNA双向启动子，在分子生物学领域和分子育种工业中产生载体产物。 构成：甘薯叶卷曲感染DNA病毒的双向启动子由序列号1的序列表示。鉴定启动子活性的方法包括：分离具有启动子活性的位点并分析顺式作用调节位点的步骤; 通过使用网关克隆到用于启动子分析的植物表达载体来重组启动子位点的步骤; 将重组的启动子位点转化到根癌土壤杆菌菌株GV3101的步骤; 并筛选转化体的步骤。
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12.发明授权애멸구 유래 재조합 그로이엘단백질 유전자 클론 및 이를이용한 벼줄무늬 잎마름병 바이러스의 면역포획역전사효소연쇄중합반응 검정방법 有权来自小褐斑病病人的重组体基因克隆和使用蛋白质蛋白质的米糠病毒免疫测定RT-PCR的检测方法
公开(公告)号：KR100822742B1
公开(公告)日：2008-04-17
申请号：KR1020070026577
申请日：2007-03-19
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6888 , C12Q1/701 , C12Q2531/113
Abstract: A recombinant protein derived from small brown planthopper is provided to be specifically coupled to thread-shaped rice stripe virus, thereby forecasting precise RSV outbreak, developing a transgenic plant having RSV resistance and being applied as various tools for detecting the RSV. A recombinant protein described in SEQ ID : NO. 2 is obtained by expressing a recombinant protein in a gene clone(deposition no. KACC-95060P) described in SEQ ID : NO. 1, which is constructed by inserting a GroEL gene isolated from small brown planthopper into an expression vector, and then purifying the protein using His-taq Ni-NTA agarose column and is characterized in that it is specifically coupled to thread-shaped RSV. A method for detecting an RSV gene comprises the steps of: (a) coating a reaction container which is PolySorp treated with the GroEL recombinant protein derived from the small brown planthopper or an antibody thereof with a coating solution; (b) treating the reaction solution with a detecting sample through coupling reaction with the with the RSV to immuno-capturing RSV particles; and (c) after isolating genes from the immuno-captured RSV particles, subjecting the isolated genes to RT-PCR to detect genes of the RSV.
Abstract translation: 提供了一种来源于小型褐飞虱的重组蛋白，特异性地与丝状水稻条纹病毒偶联，从而预测了精确的RSV疫情，开发了具有RSV抗性的转基因植物，并被用作检测RSV的各种工具。 SEQ ID NO： 2是通过在SEQ ID NO：1中描述的基因克隆（沉积编号KACC-95060P）中表达重组蛋白获得的。 1，其通过将从小褐飞虱分离的GroEL基因插入表达载体中构建，然后使用His-taq Ni-NTA琼脂糖柱纯化蛋白质，其特征在于其特异性结合于螺纹状RSV。 检测RSV基因的方法包括以下步骤：（a）用来自小褐飞虱或其抗体的GroEL重组蛋白用涂布溶液涂覆PolySorp处理的反应容器; （b）用检测样品通过与RSV的偶联反应处理反应溶液以免疫捕获RSV颗粒; 和（c）从免疫捕获的RSV颗粒中分离出基因后，将分离的基因进行RT-PCR检测RSV的基因。
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公开(公告)号：KR1020170056143A
公开(公告)日：2017-05-23
申请号：KR1020150159357
申请日：2015-11-13
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/20 , C12R1/45 , C12P21/00 , B01D15/16 , B01D57/02 , A23L1/30 , A23L1/305 , A23L3/3472 , A23L3/3571 , A23K1/00 , A23K1/16
Abstract: 본발명에따른스타필로코코스에피더미디스(R0002는황색포도상구균을포함해식중독의원인이되는다양한병원성균에대해항균활성을보이며, 더욱이항생제내성균에대해서도길항력을가지며, 해당균주유래의길항물질은광범위한온도및 pH에서안정성이우수한바, 본발명에따른균주, 이의배양물, 또는이에서유래된길항물질은항균조성물, 사료조성물, 식품조성물, 약학조성물, 의약외품조성물또는/및천연방부제조성물등의유효성분으로다양하게활용될수 있다.
Abstract translation: 由金黄色葡萄球菌表皮科科斯（R0002在根据本发明包括金黄色葡萄球菌显示出对各种病原菌引起食物中毒的抗菌活性，并且还具有关于抗生素抗性细菌，衍生自菌株的拮抗剂的道路拖 根据本发明的抗微生物组合物，食品组合物，食品组合物，药物组合物，准药品组合物和/或天然的防腐剂组合物包括温度的银矿范围和优异的pH值，应变，其培养物，或来源于此的拮抗剂稳定性条 它可以作为不同的有效成分使用
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公开(公告)号：KR1020140081103A
公开(公告)日：2014-07-01
申请号：KR1020120150480
申请日：2012-12-21
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/00
Abstract: The present invention relates to a method for screening a material for inducing resistance against plant diseases, the method comprising the steps of: (a) smearing a sample for inspection on the leaf of an indicator plant; (b) smearing a standard sample on the left half of the indicator plant of step (a); (c) leaving the leaf of step (b) in order to exhibit a local lesion; and calculating the number of local lesions exhibited on the leaf of step (c) in order to be substituted for a potency (CVT) inspection formula so that the antiviral functions of a tested material can be determined. The method according to the present invention indicates pass standards and indexes through plant antivirus active potency metrization so as to provide a means which is useful in developing environmentally-friendly agriculture materials by ensuring a standard method to inspect the activity of an antiviral agent against plant viruses. The method is also useful in objectively managing the quality of the environmentally-friendly agriculture materials.
Abstract translation: 本发明涉及一种筛选用于诱导植物病害抗性的材料的方法，所述方法包括以下步骤：（a）在指示植物的叶片上涂抹样品进行检查; （b）在步骤（a）的指示器的左半部分涂抹标准样品; （c）离开步骤（b）的叶以展示局部损伤; 并计算在步骤（c）的叶上显示的局部损伤的数量，以取代效能（CVT）检查公式，以便可以测定被测物质的抗病毒功能。 根据本发明的方法通过植物抗病毒活性效价度表示通过标准和指标，以提供一种可用于开发环保农业材料的手段，通过确保检测抗病毒剂对植物病毒的活性的标准方法 。 该方法也有利于客观地管理环保农产品的质量。
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公开(公告)号：KR1020090112596A
公开(公告)日：2009-10-28
申请号：KR1020090035784
申请日：2009-04-24
Applicant: 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
Abstract: PURPOSE: A sweet potato leaf curl infection DNA virus gene and a recombinant plasmid are provided to isolate virus and use in Korean eco system environment. CONSTITUTION: A sweet potato leaf curl infection DNA virus gene is denoted by the sequence of the sequence number 1. The gene comprises a dimer. A sweet potato leaf curl infection DNA virus (SPLCV) protein is denoted by the sequence of the sequence numbers 14 to 19.
Abstract translation: 目的：提供甘薯叶卷曲感染DNA病毒基因和重组质粒，以分离病毒，并在韩国生态系统环境中使用。 构成：甘薯叶卷曲感染DNA病毒基因由序列号1的序列表示。该基因包含二聚体。 甘薯叶卷曲感染DNA病毒（SPLCV）蛋白由序列号14〜19的序列表示。
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Patent Agency Ranking16.发明授权고추 및 파프리카에 발생하는 고추모틀바이러스,잠두위조바이러스2, 오이모자이크바이러스 및고추마일드모틀바이러스 진단용 프라이머 조합 有权家禽和辣椒粉，引物蛾病毒，豆腐病毒2，黄瓜花叶病毒和胡椒轻度斑驳病毒
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公开(公告)号：KR1020040047333A
公开(公告)日：2004-06-05
申请号：KR1020020075494
申请日：2002-11-29
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Specific primers for detecting cucumber green mottle mosaic virus are provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, detecting all kinds of cucumber green mottle mosaic virus, and improving the detection accuracy by removing nonspecific reaction with other related viruses. CONSTITUTION: The specific primers for detecting cucumber green mottle mosaic virus comprise a nucleic acid fragment selected from the nucleotide sequences set forth in SEQ ID NO: 2 to SEQ ID NO: 4 which species specifically bind with cucumber green mottle mosaic virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof; and another nucleic acid fragment selected from the nucleotide sequences set forth in SEQ ID NO: 7 to SEQ ID NO: 9 which are combined with the selected nucleic acid and species specifically bind with cucumber green mottle mosaic virus, complementary nucleotide sequences thereof, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof.
Abstract translation: 目的：提供黄瓜绿斑马赛克病毒检测的特异性引物，提高检测限度和灵敏度，比使用抗血清高出约1000倍，检测各种黄瓜绿斑马花叶病毒，通过去除与其他物质的非特异性反应提高检测精度 相关病毒。 构成：用于检测黄瓜绿斑驳花叶病毒的特异性引物包含选自SEQ ID NO：2至SEQ ID NO：4所示的核苷酸序列的核酸片段，其特异性结合黄瓜绿斑驳花叶病毒，互补核苷酸序列 及其突变核苷酸序列; 和选自与所选择的核酸和物种组合的序列号7至SEQ ID NO：9所示的核苷酸序列的另一核酸片段与黄瓜绿斑驳花叶病毒，其互补核苷酸序列和突变的 其核苷酸序列，其中突变选自碱基的缺失，碱的加入，碱的取代或其组合。
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公开(公告)号：KR100307669B1
公开(公告)日：2001-09-24
申请号：KR1019990005703
申请日：1999-02-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12N7/00
Abstract: 본발명은백합잠재바이러스(Lily symptomless virus, LSV)검정용단크론항체를분비하는융합세포인하이브리도마(Hybridoma) 와하이브리도마에의해분비되는단크론항체에관한것이다. 백합잠재바이러스는백합을재배하고있는세계어느지역에서나보편적으로감염되어있으며, 이러한바이러스감염이고품질절화백합생산은물론, 백합생산을위한우량종구생산의제한요인이되고있다. 본발명은세포융합기술을이용하여백합잠재바이러스에대해특이적으로반응하는단크론항체를개발하여대량의시료에대한바이러스의감염여부를검정할수 있도록하고, 종래의항체시약의수입에따른비경제성과수입시수송도중에야기되는변질, 적기공급등의제반문제들을해결할수 있다.
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公开(公告)号：KR101660229B1
公开(公告)日：2016-09-27
申请号：KR1020140141103
申请日：2014-10-17
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은신규한트라이코데르마하지아눔 MPA167 균주및 이의용도에관한것으로, 보다구체적으로트라이코데르마하지아눔 MPA167을이용해식물병원균을방제하는방법또는트라이코데르마하지아눔 MPA167를이용해식물생육을촉진시키는방법에관한것이다. 또한, 트라이코데르마하지아눔 MPA167 배양물로부터휘발성물질 A를생산하는방법에관한것이다. 본발명에따른트라이코데르마하지아눔 MPA167은식물병원균방제효과를가지면서동시에식물의생육을효과적으로증진시킬수 있어복합제제로서유용성을가지므로, 다양한미생물제제로생산, 보급되어화학제제를쓰지않고친환경적으로농작물을생산할수 있을것으로기대된다.
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公开(公告)号：KR101554000B1
公开(公告)日：2015-09-17
申请号：KR1020120150480
申请日：2012-12-21
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/00
Abstract: 본발명은 (a) 지표식물의잎에검증용시료를도말하는단계; (b) (a)의지표식물좌측반엽에표준시료를도말하는단계; (c) (b) 단계의잎에괴사병반(local lesion)이나타나도록방치하는단계; 및 (d) (c) 단계로잎 상에나타난괴사병반수를계수하고, 역가(CVT) 검정공식에대입하여시험물질의방제능을판독하는단계를포함하는식물병저항성유도물질의스크리닝방법에관한것이다. 본발명에따른스크리닝방법은식물항바이러스활성역가계량화를통한합격기준및 색인(index)을제시함에따라, 항식물바이러스제활성검정표준방법의확립에의한친환경농자재개발이용이한수단을제공하는효과가있고, 친환경농자재의객관적인품질관리에유용하다
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