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公开(公告)号：KR101258695B1
公开(公告)日：2013-04-29
申请号：KR1020100119377
申请日：2010-11-29
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 마늘로부터 5-하이드록시메틸-2-푸랄데하이드를 생산하는 방법에 관한 것으로, 보다 구체적으로는 (S1) 마늘을 고온숙성시키는 단계; (S2) 숙성된 마늘을 열수추출하는 단계; (S3) 열수추출물을 여과 및 농축하는 단계; (S4) 에틸아세테이트 또는 부탄올로 분획하는 단계; 및 (S5) 분획물을 분리 정제하는 단계;를 포함하는 5-하이드록시메틸-2-푸랄데하이드의 생산 방법에 대한 것이다. 본 발명에 따르면 마늘을 이용하여 순도 높은 HMF를 보다 경제적이고 용이하게 고수율로 생산할 수 있다.
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公开(公告)号：KR1020120057848A
公开(公告)日：2012-06-07
申请号：KR1020100119377
申请日：2010-11-29
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A23L27/105 , A23V2002/00 , A23V2300/14 , A23V2300/50
Abstract: PURPOSE: A producing method of 5-hydroxymethyl-2-furaldehyde using garlic is provided to enable users to easily obtain the high purity 5-hydroxymethyl-2-furaldehyde with the high yield. CONSTITUTION: A producing method of 5-hydroxymethyl-2-furaldehyde using garlic comprises the following steps: high-temperature aging the garlic at 70-90 deg C for 15-40 days by sealing the garlic with a polyethylene film; hot-water extracting the aged garlic; filtering and concentrating the garlic hot-water extract; fractionating the concentrated garlic hot-water extract using ethyl acetate or butanol; separating and purifying the obtained fractions; and freeze-drying the fractions. The concentration of the garlic hot-water extract is processed by using a rotational vacuum concentrator. The fractionating process of the concentrated garlic hot-water extract is performed by using the high-performance liquid chromatography of the reverse-phase column chromatography method.
Abstract translation: 目的：提供使用大蒜的5-羟甲基-2-糠醛的制备方法，使用户能够以高产率容易地获得高纯度的5-羟甲基-2-糠醛。 构成：使用大蒜制备5-羟甲基-2-糠醛的方法包括以下步骤：通过用聚乙烯膜密封大蒜，使大蒜在70-90℃下高温老化15-40天; 热水提取老化大蒜; 过滤浓缩大蒜热水提取物; 使用乙酸乙酯或丁醇对浓缩的大蒜热水提取物进行分馏; 分离和纯化得到的级分; 并冷冻干燥该级分。 通过使用旋转式真空浓缩器处理大蒜热水提取物的浓度。 浓缩大蒜热水提取物的分馏过程通过使用反相柱色谱法的高效液相色谱法进行。
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公开(公告)号：KR1020030022564A
公开(公告)日：2003-03-17
申请号：KR1020010055724
申请日：2001-09-11
Applicant: 대한민국(농촌진흥청장)
IPC: C09K15/06
CPC classification number: C09K15/08 , C07C47/565
Abstract: PURPOSE: Provided is an antioxidant comprising 3,4,5-trihydroxybenzaldehyde as an active ingredient, which has an improved free radical elimination power than that of existing material, and excellent anti-acidification of pig fat and palm oil, and thus can be widely used as an antioxidant such as additives for fat and fatty oil processed foods. CONSTITUTION: The antioxidant comprises 3,4,5-trihydroxybenzaldehyde as an effective ingredient. The 3,4,5-trihydroxybenzaldehyde is extracted from stem and leaf of Geum japonicum. Fat and fatty oil processed foods comprise an extract or dry powder of Geum japonicum comprising 3,4,5-trihydroxybenzaldehyde.
Abstract translation: 目的：提供一种抗氧化剂，其包含3,4,5-三羟基苯甲醛作为活性成分，其具有比现有材料更好的自由基消除能力，以及猪脂肪和棕榈油的优异的抗酸化，因此可广泛地 用作脂肪和脂肪油加工食品的添加剂等抗氧化剂。 构成：抗氧化剂为3,4,5-三羟基苯甲醛作为有效成分。 3,4,5-三羟基苯甲醛从天麻茎叶提取。 脂肪和脂肪油加工食品包括含有3,4,5-三羟基苯甲醛的天麻提取物或干粉。
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公开(公告)号：KR100341798B1
公开(公告)日：2002-06-26
申请号：KR1019990032025
申请日：1999-08-04
Applicant: 대한민국(농촌진흥청장)
IPC: C07C69/732
Abstract: 본발명은방아잎(, giant hyssop)을음건또는동결건조시키고분쇄한분말로부터추출액을수득하여이를여과시키고, 흡착크로마토그래피및 겔크로마토그래피를실시하는공정을포함하는로즈마린산을분리하는방법에관한것이다. 본발명의로즈마린산분리방법은방아잎을건조시키고분쇄하는공정; 분쇄된방아잎중량에대하여 2배내지 6배의물을가하고추출하여, 방아잎추출액을수득하는공정; 추출액을여과하고흡착크로마토그래피를실시한다음, 활성분획을농축시켜로즈마린산농축액을수득하는공정; 및, 전기농축액으로겔 크로마토그래피를실시하고, 로즈마린산을함유한분획을건조시켜고순도의로즈마린산을분리하는공정을포함한다. 본발명에의하면, 로즈마린산의손실을효과적으로줄이면서, 간단한공정에의하여고순도로즈마린산을분리할수 있다. 따라서, 본발명이로즈마린산을유효성분으로하는항산화물질을개발하는데효과적으로기여할수 있는바, 로즈마린산을식품이나의약품의첨가물로이용하는등 로즈마린산의산업적활용도를높일수 있다.
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公开(公告)号：KR100270927B1
公开(公告)日：2000-11-01
申请号：KR1019980013096
申请日：1998-04-13
Applicant: 대한민국(농촌진흥청장)
IPC: C07K14/43
Abstract: PURPOSE: An antiviral protein CAP30 separated from Chenopodium album var. is provided, which can be used as antiviral bioagricultural drugs or human antiviral medicines. CONSTITUTION: The antiviral protein CAP30 having 30,000 dalton of molecular weight is isolated by the steps of: grinding the leaves of Chenopodium album var. and extracting it with a buffer solution A containing 50 mM of sodium chloride, 0.2 mM of PMSF, 20 mM of Tris-HCl, 1mM of EDTA, and 10 mM of beta-mercaptoethanol; filtering and centrifuging the extract and collecting the supernatant; adding 30 to 80% of ammonium sulfate and dialyzing it with the buffer solution A; passing the concentrate through the DE-52 cellulose column and subjecting it to linear gradient of buffer solution A to separate the active fragments; and purifying the active fragments by subjecting it to chromatography. The gene encoding the antiviral protein CAP30 is sequenced and its nucleotide sequence is represented by sequence ID No. 1.
Abstract translation: 目的：从Chenopodium album var。中分离出抗病毒蛋白CAP30。 可用作抗病毒生物学药物或人类抗病毒药物。 构成：通过以下步骤分离具有30,000道尔顿分子量的抗病毒蛋白质CAP30：研磨藜属植物的叶子。 并用含有50mM氯化钠，0.2mM PMSF，20mM Tris-HCl，1mM EDTA和10mMβ-巯基乙醇的缓冲溶液A萃取; 过滤并离心提取物并收集上清液; 加入30〜80％的硫酸铵，用缓冲溶液A透析; 使浓缩物通过DE-52纤维素柱，并使其经历缓冲溶液A的线性梯度以分离活性片段; 并通过色谱法纯化活性片段。 对编码抗病毒蛋白CAP30的基因进行测序，其核苷酸序列由序列号1表示。
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Patent Agency Ranking
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公开(公告)号：KR1020150051631A
公开(公告)日：2015-05-13
申请号：KR1020130133350
申请日：2013-11-05
Applicant: 주식회사 성균바이오텍 , 성균관대학교산학협력단 , 대한민국(농촌진흥청장)
IPC: A61K36/899 , A61P19/10 , A61P19/00
Abstract: 본발명은흑미추출물을유효성분으로하는골다공증, 골형성장애또는골절등의골대사질환의예방또는치료용조성물에관한것으로, 본발명의갈대추출물은조골세포의분화에관련된유전자인 ALP, osterix, RUNX2 발현을증진시키고, 난소절제골다공증동물모델에서골밀도(BMD) 및골강도를증가시켜, 골대사질환에유용한의약품또는건강기능식품으로이용될수 있다.
Abstract translation: 本发明涉及含有黑米提取物作为预防或治疗骨质疏松，骨形成障碍，骨折等代谢性骨病的有效成分的组合物。 本发明的芦苇提取物促进与成骨细胞分化相关的ALP，osterix和RUNX2的表达，并且增加切除卵巢的骨质疏松症动物模型中的骨矿物质密度（BMD）和骨强度，因此， 可用作有益于代谢性骨疾病的药用产品或健康功能食品。
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公开(公告)号：KR1020140077237A
公开(公告)日：2014-06-24
申请号：KR1020120138951
申请日：2012-12-03
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A61K36/73 , A23V2200/328 , A23V2200/332
Abstract: A composition comprising an Agrimonia pilosa extract is provided. The composition according to an embodiment of the present invention comprises the Agrimonia pilosa extract and has an effect of alleviating and preventing insulin resistance syndrome which can be caused by an intake of food containing fat.
Abstract translation: 提供了包含龙卷风提取物的组合物。 根据本发明的一个实施方案的组合物包含龙胆草提取物，并且具有减轻和预防由摄入含有食物的脂肪引起的胰岛素抵抗综合征的作用。
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19.发明公开5-하이드록시메틸-2-푸랄데하이드(5-ｈｙｄｒｏｘｙｍｅｔｈｙｌ-2-ｆｕｒａｌｄｅｈｙｄｅ) 저함유 숙성마늘 추출물 및 이의 제조방법 有权低5-羟基甲基-2-呋喃二恶烷黑加味提取物及其生产方法
公开(公告)号：KR1020120040543A
公开(公告)日：2012-04-27
申请号：KR1020100102018
申请日：2010-10-19
Applicant: 대한민국(농촌진흥청장)
CPC classification number: A23L2/04 , A23L2/08 , A23P10/20 , A23V2002/00 , A23V2300/14 , A23V2300/50
Abstract: PURPOSE: An aged garlic extract with the low content of 5-hydroxymethyl-2-furaldehyde, and a producing method thereof are provided to reduce the content of the 5-hydroxymethyl-2-furaldehyde by aging the garlic in the proper temperature and humidity. CONSTITUTION: A producing method of an garlic extract with the low content of 5-hydroxymethyl-2-furaldehyde comprises the following steps: aging garlic at 50-60 deg C for 15-35 days in the humidity of 70-90%; mixing the aged garlic with water in a water ratio of 1:3-5 before crushing; and refluxing and cooling the crushed mixture before extracting and filtering to obtain the extract. The aged garlic contains less than 1mg/g of 5-hydroxymethyl-2-furaldehyde, and more than 35%/g.DW of fructose.
Abstract translation: 目的：提供5-羟甲基-2-糠醛含量低的老化大蒜提取物及其制备方法，通过在适当的温度和湿度下老化大蒜来降低5-羟甲基-2-糠醛的含量。 构成：5-羟甲基-2-糠醛含量低的大蒜提取物的生产方法包括以下步骤：在70-60％的湿度下将大蒜在50-60℃老化15-35天; 将老化大蒜与水比例为1：3-5混合，然后粉碎; 回流并冷却粉碎的混合物，然后提取和过滤，得到提取物。 老化大蒜含有少于1mg / g的5-羟甲基-2-糠醛，超过35％/ g.DW的果糖。
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20.发明公开안토시아닌 생합성 억제 활성을 가진 ＭＹＢ６０ 유전자와상기 유전자가 도입된 형질전환 상추 및 그 제조방법 有权用于抑制蒽醌生物合成的MYB60基因，MYB60基因被转移的转基因及其制备方法
公开(公告)号：KR1020070110615A
公开(公告)日：2007-11-20
申请号：KR1020060043300
申请日：2006-05-15
Applicant: 대한민국(농촌진흥청장)
Abstract: A MYB60 gene is provided to inhibit biosynthesis of anthocyanin, so that it is useful for change of flower color of flower plants and for development of new varieties of flower plants. A transgenic lettuce containing the same MYB60 gene is also provided to verify anthocyanin biosynthesis-inhibiting effects of the gene through phenotype by using a plant system. A MYB60 gene inhibiting biosynthesis of anthocyanin has the nucleotide sequence of SEQ ID NO:1. A vector 22103-MYB60 contains the MYB60 gene. A transformed Agrobacterium tumefaciens EHA105(KACC 95045P) is produced with the vector 22103-MYB60. A method for preparing a transgenic lettuce comprises the steps of: (a) preparing an expression vector containing the MYB60 gene and a basta resistant bar gene as a selection marker; (b) producing Agrobacterium tumefaciens EHA105(KACC 95045P) by transforming with the expression vector; (c) co-culturing cotyledon of a lettuce plant with the transformed Agrobacterium tumefaciens EHA105(KACC 95045P); (d) selecting shoots of lettuce plant from a selection medium and inducing plants from the shoots in plant-inducing medium; and (e) transplanting the induced lettuce plants to an in vitro medium and the soil to acclimate them.
Abstract translation: 提供了一种MYB60基因来抑制花青素的生物合成，因此可用于改变花卉植物的花色和开发新品种的花卉植物。 还提供了含有相同MYB60基因的转基因莴苣，以通过使用植物系统来验证基因通过表型的花青素生物合成抑制作用。 抑制花青素生物合成的MYB60基因具有SEQ ID NO：1的核苷酸序列。 载体22103-MYB60含有MYB60基因。 用载体22103-MYB60生产转化的根癌土壤杆菌EHA105（KACC 95045P）。 一种制备转基因莴苣的方法包括以下步骤：（a）制备含有MYB60基因和巴斯坦耐药基因作为选择标记的表达载体; （b）通过用表达载体转化产生根癌土壤杆菌EHA105（KACC 95045P）; （c）用转化的根癌土壤杆菌EHA105（KACC 95045P）共同培养莴苣植物的子叶; （d）从选择培养基中选择莴苣植物芽，并诱导植物诱导培养基中的芽; 和（e）将诱导的莴苣植物移植到体外培养基和土壤以适应它们。
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