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公开(公告)号：KR100842446B1
公开(公告)日：2008-07-01
申请号：KR1020070035744
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
Abstract: An SSR(simple sequence repeat) primer pair derived from ginger is provided to detect DNA polymorphism of the ginger effectively and be very usefully used for DNA profiling of the ginger, thereby analyzing whether a newly introduced genetic resource is duplicated with a conventional resource and securing novelty of the newly introduced genetic resource by evaluating the genetic source of the ginger efficiently. An SSR primer pair is selected from the group consisting of: a primer pair of SEQ ID : NOs. 1 and 2; a primer pair of SEQ ID : NOs. 3 and 4; a primer pair of SEQ ID : NOs. 5 and 6; a primer pair of SEQ ID : NOs. 7 and 8; a primer pair of SEQ ID : NOs. 9 and 10; a primer pair of SEQ ID : NOs. 11 and 12; a primer pair of SEQ ID : NOs. 13 and 14; and a primer pair of SEQ ID : NOs. 15 and 16 and is derived from ginger(Zingiber officinale Rosc.). A method for detecting DNA polymorphism of the ginger comprises the steps of: (a) extracting genome DNA from the ginger; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A method for identifying species of the ginger comprises the steps of: (a) extracting genome DNA from the ginger and a control group ginger species, respectively; (b) subjecting each of the extracted genome DNA to PCR using the SSR primer pair; (c) isolating each of PCR products obtained from the step(b) by size; and (d) comparing isolation results by the size of the ginger with that of the control group. A kit for detecting the DNA polymorphism of the ginger or identifying species of the ginger comprises the SSR primer pair.
Abstract translation: 提供从姜中获得的SSR（简单序列重复）引物对，有效检测生姜的DNA多态性，非常有用地用于生姜的DNA分析，从而分析新引入的遗传资源是否与传统资源重复 通过有效地评估姜的遗传来源，新引入的遗传资源的新颖性。 SSR引物对选自SEQ ID NO：的引物对。 1和2; SEQ ID NO：1的引物对。 3和4; SEQ ID NO：1的引物对。 5和6; SEQ ID NO：1的引物对。 7和8; SEQ ID NO：1的引物对。 9和10; SEQ ID NO：1的引物对。 11和12; SEQ ID NO：1的引物对。 13和14; 和SEQ ID NO：的引物对。 15和16，来自姜（Zingiber officinale Rosc。）。 用于检测生姜的DNA多态性的方法包括以下步骤：（a）从姜提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 用于鉴定姜的种类的方法包括以下步骤：（a）从姜和对照组姜分别提取基因组DNA; （b）使用SSR引物对对所提取的基因组DNA进行PCR; （c）通过大小分离从步骤（b）获得的每个PCR产物; 和（d）将分离结果与姜的大小与对照组的大小进行比较。 用于检测生姜的DNA多态性或识别姜的种类的试剂盒包含SSR引物对。
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公开(公告)号：KR100803392B1
公开(公告)日：2008-02-14
申请号：KR1020060076809
申请日：2006-08-14
Applicant: 대한민국(농촌진흥청장)
Abstract: An SSR primer pair is provided to be able to be very usefully used for preparing a DNA profile of Coix lachryma-jobi by effectively detecting a DNA polymorphism and effectively analyze the redundancy with conventional resources and establish the novelty when a novel genetic source is introduced by efficiently evaluating the genetic source of the Coix lachryma-jobi. An SSR primer pair derived from Coix lachryma-jobi is selected from the group consisting of a primer pair described as SEQ ID : NOs. 1 and 2; a primer pair described as SEQ ID : NOs. 3 and 4; a primer pair described as SEQ ID : NOs. 5 and 6; a primer pair described as SEQ ID : NOs. 7 and 8; a primer pair described as SEQ ID : NOs. 9 and 10; a primer pair described as SEQ ID : NOs. 11 and 12; a primer pair described as SEQ ID : NOs. 13 and 14; a primer pair described as SEQ ID : NOs. 15 and 16; a primer pair described as SEQ ID : NOs. 17 and 18; a primer pair described as SEQ ID : NOs. 19 and 20; a primer pair described as SEQ ID : NOs. 21 and 22; a primer pair described as SEQ ID : NOs. 23 and 24; a primer pair described as SEQ ID : NOs. 25 and 26; a primer pair described as SEQ ID : NOs. 27 and 28; a primer pair described as SEQ ID : NOs. 29 and 30; a primer pair described as SEQ ID : NOs. 31 and 32; and a primer pair described as SEQ ID : NOs. 33 and 34. A method for detecting a DNA polymorphism of Coix lachryma-jobi comprises the steps of: (a) extracting a genome DNA from the Coix lachryma-jobi; (b) performing a PCR using the extracted genome DNA as a template and the SSR primer pair; and (c) isolating PCR products by size.
Abstract translation: 提供了一种SSR引物对，可以非常有效地用于通过有效检测DNA多态性并有效地分析冗余与传统资源，并建立新颖的遗传源由新的遗传源引入时，通过 有效评估枸杞子的遗传来源。 衍生自枸杞子的SSR引物对选自如SEQ ID NO：所示的引物对。 1和2; 如SEQ ID NO：所示的引物对。 3和4; 如SEQ ID NO：所示的引物对。 5和6; 如SEQ ID NO：所示的引物对。 7和8; 如SEQ ID NO：所示的引物对。 9和10; 如SEQ ID NO：所示的引物对。 11和12; 如SEQ ID NO：所示的引物对。 13和14; 如SEQ ID NO：所示的引物对。 15和16; 如SEQ ID NO：所示的引物对。 17和18; 如SEQ ID NO：所示的引物对。 19和20; 如SEQ ID NO：所示的引物对。 21和22; 如SEQ ID NO：所示的引物对。 23和24; 如SEQ ID NO：所示的引物对。 25和26; 如SEQ ID NO：所示的引物对。 27和28; 如SEQ ID NO：所示的引物对。 29和30; 如SEQ ID NO：所示的引物对。 31和32; 和如SEQ ID NO：所示的引物对。 一种检测枸杞子DNA多态性的方法，包括以下步骤：（a）从枸杞子提取基因组DNA; （b）使用提取的基因组DNA作为模板和SSR引物对进行PCR; 和（c）按大小分离PCR产物。
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公开(公告)号：KR100769368B1
公开(公告)日：2007-10-31
申请号：KR1020060094922
申请日：2006-09-28
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/686
Abstract: An SSR(simple sequence repeat) primer derived from Setaria italica (L.) Beauv. using a biotin-streptavidin capturing method is provided to be very effectively detect the DNA polymorphism of the Setaria italica (L.) Beauv. and be usefully used for preparing a DNA profile of the Setaria italica (L.) Beauv.. An SSR primer pair derived from Setaria italica (L.) Beauv. has two sequences selected from the group consisting of SEQ ID : NOs. 1-46 such as GB-Slt-002, GB-Slt-012, GB-Slt-018, GB-Slt-019, GB-Slt-030, GB-Slt-031, GB-Slt-039, GB-Slt-044, GB-Slt-046, GB-Slt-051, GB-Slt-054, GB-Slt-056, GB-Slt-059, GB-Slt-066, GB-Slt-068, GB-Slt-077, GB-Slt-091, GB-Slt-105, GB-Slt-108, GB-Slt-113, GB-Slt-117, GB-Slt-130 and GB-Slt-135. A method for identifying species of Setaria italica (L.) Beauv. comprises the steps of: (a) extracting genome DNAs from Setaria italica (L.) Beauv. and a control Setaria italica (L.) Beauv. species; (b) performing PCR on each of the extracted genome DNAs using the SSR primer pair; (c) isolating each of the PCR products by size; and (d) comparing the isolation results of the Setaria italica (L.) Beauv. and the control Setaria italica (L.) Beauv. species. A kit for detecting DNA polymorphism or a kit for identifying species of Setaria italica (L.) Beauv. comprises the SSR primer pair.
Abstract translation: 来自Setaria italica（L.）Beauv的SSR（简单序列重复）引物。 提供使用生物素 - 链霉抗生物素蛋白捕获方法非常有效地检测狗尾草（L.）Beauv的DNA多态性。 并且有用地用于制备狗尾草（L.）Beauv的DNA谱。来自Setaria italica（L.）Beauv的SSR引物对。 具有选自SEQ ID：NO的两个序列。 1-46例如GB-Slt-002，GB-Slt-012，GB-Slt-018，GB-Slt-019，GB-Slt-030，GB-Slt-031，GB-Slt-039，GB-Slt -044，GB-Slt-046，GB-Slt-051，GB-Slt-054，GB-Slt-056，GB-Slt-059，GB-Slt-066，GB-Slt-068，GB-Slt-077 ，GB-Slt-091，GB-Slt-105，GB-Slt-108，GB-Slt-113，GB-Slt-117，GB-Slt-130和GB-Slt-135。 一种鉴定狗尾草属（L.）Beauv种的方法。 包括以下步骤：（a）从Setaria italica（L.）Beauv提取基因组DNA。 和对照Setaria italica（L.）Beauv。 种类; （b）使用SSR引物对对每个提取的基因组DNA进行PCR; （c）按大小分离每个PCR产物; 和（d）比较狗尾草（L.）Beauv的分离结果。 和对照Setaria italica（L.）Beauv。 种类。 用于检测DNA多态性的试剂盒或用于鉴定狗尾草属（L.）Beauv的物种的试剂盒。 包括SSR引物对。
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Patent Agency Ranking
公开(公告)号：KR100769366B1
公开(公告)日：2007-10-31
申请号：KR1020060094921
申请日：2006-09-28
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/686
Abstract: An SSR(simple sequence repeat) primer pair is provided to be very usefully used for preparing a DNA profile of Vigna radiata L. by effectively detecting the DNA polymorphism of the Vigna radiata L., thereby capable of performing redundancy analysis with conventional resources when introducing a novel genetic resource and determining species of the Vigna radiata L.. An SSR primer pair has two sequences selected from the group consisting of SEQ ID : NOs. 1-14 and is derived from Vigna radiata L.. A method for detecting DNA polymorphism of Vigna radiata L. comprises the steps of: (a) extracting genome DNA from Vigna radiata L.; (b) performing PCR on the extracted genome DNA as a template using the SSR primer pair; and (c) isolating the PCR product by size. A method for identifying species of Vigna radiata L. comprises the steps of: (a) extracting genome DNAs from Vigna radiata L. and a control Vigna radiata L. species; (b) performing PCR on each of the extracted genome DNAs using the SSR primer pair; (c) isolating each of the PCR products by size; and (d) comparing the isolation results of the Vigna radiata L. and the control Vigna radiata L. species.
Abstract translation: 提供SSR（简单序列重复）引物对非常有用地用于通过有效地检测Vigna radiata L.的DNA多态性来制备Vigna radiata L.的DNA谱，从而能够在引入时用常规资源进行冗余分析 一种新型遗传资源和确定Vigna radiata L的物种.SSSR引物对具有选自SEQ ID NO： 1-14，衍生自Vigna radiata L。一种用于检测鞭毛虫DNA的多态性的方法，包括以下步骤：（a）从梵氏菌提取基因组DNA。 （b）使用SSR引物对在提取的基因组DNA上作为模板进行PCR; 和（c）按大小分离PCR产物。 一种用于鉴定芸香属种的方法，包括以下步骤：（a）从梵氏菌（Vigna radiata L.）提取基因组DNA，以及控制芸苔（Vigna radiata L.）种; （b）使用SSR引物对对每个提取的基因组DNA进行PCR; （c）按大小分离每个PCR产物; 和（d）比较Vigna radiata L.和对照Vigna radiata L.物种的分离结果。
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公开(公告)号：KR101047964B1
公开(公告)日：2011-07-12
申请号：KR1020090066472
申请日：2009-07-21
Applicant: 대한민국(농촌진흥청장) , 대윤계기산업 주식회사
CPC classification number: G01N33/02
Abstract: 본 발명은 포켓용 짠맛센서에 관한 것으로, 내부에 회로기판 및 배터리가 내장되는 중공형 몸체; 일단이 몸체의 단부에 삽입되고, 타단에는 짠맛측정센서구멍이 형성된 돌출부와 온도센서구멍이 형성된 함몰부가 형성되어 있는 셀블록; 셀블록의 짠맛측정센서구멍에 삽입되고 일단이 회로기판에 연결되는 짠맛측정센서; 셀블록의 온도센서구멍에 삽입되고 일단이 회로기판에 연결되는 온도센서; 셀블록의 반대측 몸체 단부에 설치되고 회로기판과 배터리에 연결되는 온/오프스위치; 회로기판에 연결되어 있는 발광램프; 온/오프스위치 측 몸체의 일단에 결합되거나 셀블록에 결합되고, 내부에 온/오프스위치 작동용 작동편이 형성된 몸체커버;로 이루어진다.
따라서, 짠맛센서의 클립을 포켓에 끼워서 간편하게 휴대할 수 있고, 몸체커버를 셀블록에 닫기 위해 몸체로부터 분리시키면 온/오프스위치가 자동으로 꺼지므로 배터리가 불필요하게 소모되지 않으며, 온도센서가 외부로 노출되어 있으므로 시료의 온도측정이 신속하고 정확하게 이루어질 뿐 아니라, 측정된 음식물의 짠맛값에 따라 서로 다른 색상이 발광되면서 이를 간편하게 식별할 수 있고, 발광램프의 점등시 몸체커버의 난반사돌기들에 의해 그 빛이 몸체커버의 단부 전체로 확산된다.
클립, 셀블록, 온/오프스위치, 발광램프, 몸체커버, 난반사돌기, 작동편Abstract translation: 本发明是一个空心体，其是要，电路板和内置于咸味传感器用于口袋的内部的电池; 一端插入到所述主体的端部，形成在突出部的另一端咸传感器孔和形成在该凹部的温度传感器孔在单元块形成; 一种盐味测量传感器，插入电池组的盐味测量传感器孔中，并且其一端连接到电路板; 温度传感器，插入电池块的温度传感器孔中，并且其一端连接到电路板; 安装在电池块的相对本体端并连接到电路板和电池的开/关开关; 连接到电路板的发光灯; 以及与开/关开关侧主体的一端耦合或与电池块耦合并且具有形成在其中的用于开/关切换操作的操作件的主体盖。
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公开(公告)号：KR1020100110525A
公开(公告)日：2010-10-13
申请号：KR1020090028899
申请日：2009-04-03
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A cryopreservation and regeneration method for Chrysanthemum shoot is provided to preserve Chrysanthemum using vitrification solution and loading solution. CONSTITUTION: A cryopreservation for cryopreservation shoot comprises: a step of extracting laterial bud of in vitro cultured cryopreservation; a step of pre-culturing in 0.3M-0.5M-0.7M of sucrose medium; a step of culturing in a loading solution(17.5% of glycerol and 17.5% of sucrose) for 40-50 minutes; a step of shaking and dehydrating the Chrysanthemum; and a step of rapidly-freezing in the liquid nitrogen.
Abstract translation: 目的：提供菊花芽的冷冻保存和再生方法，用玻璃化溶液和加载溶液保存菊花。 构成：冷冻保存芽的冷冻保存包括：提取体外培养的低温保存的材料芽的步骤; 在0.3M-0.5M-0.7M蔗糖培养基中预培养的步骤; 在负载溶液（17.5％甘油和17.5％蔗糖）中培养40-50分钟的步骤; 菊花摇摆脱水的一步; 以及在液氮中快速冷冻的步骤。
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公开(公告)号：KR1020080092187A
公开(公告)日：2008-10-15
申请号：KR1020070035743
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2600/156
Abstract: An SSR(simple sequence repeat) primer pair derived from Amaranth is provided to detect DNA polymorphism of the Amaranth effectively and be very usefully used for DNA profiling of the Amaranth, thereby analyzing whether a newly introduced genetic resource is duplicated with a conventional resource and securing novelty of the newly introduced genetic resource by evaluating the genetic source of the Amaranth efficiently. An SSR primer pair is selected from the group consisting of: a primer pair of SEQ ID : NOs. 1 and 2; a primer pair of SEQ ID : NOs. 3 and 4; a primer pair of SEQ ID : NOs. 5 and 6; a primer pair of SEQ ID : NOs. 7 and 8; a primer pair of SEQ ID : NOs. 9 and 10; a primer pair of SEQ ID : NOs. 11 and 12; a primer pair of SEQ ID : NOs. 13 and 14; a primer pair of SEQ ID : NOs. 15 and 16; a primer pair of SEQ ID : NOs. 17 and 18; a primer pair of SEQ ID : NOs. 19 and 20; a primer pair of SEQ ID : NOs. 21 and 22; and a primer pair of SEQ ID : NOs. 23 and 24 and is derived from Amaranth(Amaranthus spp.). A method for detecting DNA polymorphism of the Amaranth comprises the steps of: (a) extracting genome DNA from the Amaranth; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A method for identifying species of the Amaranth comprises the steps of: (a) extracting genome DNA from the Amaranth and a control group Amaranth species, respectively; (b) subjecting each of the extracted genome DNA to PCR using the SSR primer pair; (c) isolating each of PCR products obtained from the step(b) by size; and (d) comparing isolation results by the size of the Amaranth with that of the control group. A kit for detecting the DNA polymorphism of the Amaranth or identifying species of the Amaranth comprises the SSR primer pair.
Abstract translation: 提供了从苋菜属得到的SSR（简单序列重复）引物对，有效地检测了苋菜的DNA多态性，非常有用地用于苋菜的DNA分析，从而分析新引入的遗传资源是否与常规资源重复 通过有效地评估苋菜的遗传来源，新引入的遗传资源的新颖性。 SSR引物对选自SEQ ID NO：的引物对。 1和2; SEQ ID NO：1的引物对。 3和4; SEQ ID NO：1的引物对。 5和6; SEQ ID NO：1的引物对。 7和8; SEQ ID NO：1的引物对。 9和10; SEQ ID NO：1的引物对。 11和12; SEQ ID NO：1的引物对。 13和14; SEQ ID NO：1的引物对。 15和16; SEQ ID NO：1的引物对。 17和18; SEQ ID NO：1的引物对。 19和20; SEQ ID NO：1的引物对。 21和22; 和SEQ ID NO：的引物对。 23和24，来自苋属（Amaranthus spp。）。 一种检测苋菜DNA多态性的方法，包括以下步骤：（a）从苋菜提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 一种鉴定苋菜品种的方法，包括以下步骤：（a）分别从苋菜和对照组苋菜提取基因组DNA; （b）使用SSR引物对对所提取的基因组DNA进行PCR; （c）通过大小分离从步骤（b）获得的每个PCR产物; 和（d）将隔离结果与苋菜的大小与对照组的大小进行比较。 用于检测苋菜或苋菜品种的DNA多态性的试剂盒包含SSR引物对。
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公开(公告)号：KR100842434B1
公开(公告)日：2008-07-01
申请号：KR1020070035742
申请日：2007-04-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q2600/156
Abstract: An SSR(simple sequence repeat) primer pair derived from ginseng is provided to detect DNA polymorphism of the ginseng effectively and be very usefully used for DNA profiling of the ginseng. An SSR primer pair is selected from the group consisting of: a primer pair of SEQ ID : NOs. 1 and 2; a primer pair of SEQ ID : NOs. 3 and 4; a primer pair of SEQ ID : NOs. 5 and 6; a primer pair of SEQ ID : NOs. 7 and 8; a primer pair of SEQ ID : NOs. 9 and 10; a primer pair of SEQ ID : NOs. 11 and 12; a primer pair of SEQ ID : NOs. 13 and 14; a primer pair of SEQ ID : NOs. 15 and 16; a primer pair of SEQ ID : NOs. 17 and 18; a primer pair of SEQ ID : NOs. 19 and 20; a primer pair of SEQ ID : NOs. 21 and 22; a primer pair of SEQ ID : NOs. 23 and 24; a primer pair of SEQ ID : NOs. 25 and 26; a primer pair of SEQ ID : NOs. 27 and 28; a primer pair of SEQ ID : NOs. 29 and 30; a primer pair of SEQ ID : NOs. 31 and 32; a primer pair of SEQ ID : NOs. 33 and 34; a primer pair of SEQ ID : NOs. 35 and 36; a primer pair of SEQ ID : NOs. 37 and 38; a primer pair of SEQ ID : NOs. 39 and 40; a primer pair of SEQ ID : NOs. 41 and 42; and a primer pair of SEQ ID : NOs. 43 and 44 and is derived from ginseng(Panax ginseng C. A. Meyer). A method for detecting DNA polymorphism of the ginseng comprises the steps of: (a) extracting genome DNA from the ginseng; (b) subjecting the extracted genome DNA as a template to PCR using the SSR primer pair; and (c) isolating PCR products obtained from the step(b) by size. A kit for detecting the DNA polymorphism of the ginseng or identifying species of the ginseng comprises the SSR primer pair.
Abstract translation: 提取人参中的SSR（简单序列重复）引物对，有效地检测人参的DNA多态性，非常有用地用于人参DNA分析。 SSR引物对选自SEQ ID NO：的引物对。 1和2; SEQ ID NO：1的引物对。 3和4; SEQ ID NO：1的引物对。 5和6; SEQ ID：NO的引物对。 7和8; SEQ ID NO：1的引物对。 9和10; SEQ ID NO：1的引物对。 11和12; SEQ ID NO：1的引物对。 13和14; SEQ ID NO：1的引物对。 15和16; SEQ ID NO：1的引物对。 17和18; SEQ ID NO：1的引物对。 19和20; SEQ ID NO：1的引物对。 21和22; SEQ ID NO：1的引物对。 23和24; SEQ ID NO：1的引物对。 25和26; SEQ ID NO：1的引物对。 27和28; SEQ ID NO：1的引物对。 29和30; SEQ ID NO：1的引物对。 31和32; SEQ ID NO：1的引物对。 33和34; SEQ ID NO：1的引物对。 35和36; SEQ ID NO：1的引物对。 37和38; SEQ ID NO：1的引物对。 39和40; SEQ ID NO：1的引物对。 41和42; 和SEQ ID NO：的引物对。 43和44，来源于人参（人参，A.Meyer）。 一种检测人参DNA多态性的方法，包括以下步骤：（a）从人参中提取基因组DNA; （b）使用SSR引物对将提取的基因组DNA作为模板进行PCR; 和（c）通过尺寸分离由步骤（b）获得的PCR产物。 用于检测人参的DNA多态性或鉴定人参物种的试剂盒包括SSR引物对。
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