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公开(公告)号：KR1020100015187A
公开(公告)日：2010-02-12
申请号：KR1020080076124
申请日：2008-08-04
Applicant: 대한민국(농촌진흥청장) , 성균관대학교산학협력단
Abstract: PURPOSE: A novel cholera vaccine containing an E2 antigen gene or transgenic plant expressing the gene is provided to produce an antibody to the E2 antigen and prevent cattle sickness. CONSTITUTION: An E2 antigen gene of swine cholera protein contains ER-tagged sequence or ER-retention sequence. The E2 antigen gene contains a sequence of sequence number 1. An E2 antigen protein contains amino acid sequence of sequence number 2. An expression vector for transforming a plant which is able to express E2 antigen contains the E2 antigen gene. The expression vector is E2/pB7WG2D(deposit number KACC 95087P). A plant which expresses the E2 antigen gene includes crop for livestock feed.
Abstract translation: 目的：提供含有E2抗原基因或表达该基因的转基因植物的新型霍乱疫苗，以产生E2抗原的抗体并防止牛病。 构成：猪霍乱蛋白的E2抗原基因含有ER标记的序列或ER-保留序列。 E2抗原基因含有序列号1的序列.E2抗原蛋白含有序列号2的氨基酸序列。用于转化能够表达E2抗原的植物的表达载体含有E2抗原基因。 表达载体为E2 / pB7WG2D（保藏号KACC 95087P）。 表达E2抗原基因的植物包括家畜饲料作物。
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公开(公告)号：KR101611417B1
公开(公告)日：2016-04-12
申请号：KR1020140093289
申请日：2014-07-23
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은선충유전자인마이오신라이트체인(myosin light chain) 유전자및 이의용도에관한것으로, 구체적으로마이오신라이트체인유전자에대한 RNAi 염기서열을포함하는뿌리혹선충저항성재조합벡터를이용하여뿌리혹선충저항성을가지는형질전환식물체를제작하였으므로다양한식물체에서선충에의한피해를줄일수 있는기반기술로유용하게이용될것이다.
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公开(公告)号：KR101546451B1
公开(公告)日：2015-08-25
申请号：KR1020130139848
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/63 , C12N15/113 , A01H5/00
Abstract: 본발명은선충유전자인프리폴딘 2(prefoldin 2) 유전자및 이의용도에관한것으로, 구체적으로프리폴딘 2 유전자에대한 RNAi 염기서열을포함하는뿌리혹선충저항성재조합벡터를이용하여뿌리혹선충저항성을가지는형질전환식물체를제작하였으므로다양한식물체에서선충에의한피해를줄일수 있는기반기술로유용하게이용될것이다.
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公开(公告)号：KR1020140135887A
公开(公告)日：2014-11-27
申请号：KR1020130055851
申请日：2013-05-16
Applicant: 대한민국(농촌진흥청장)
IPC: C12N9/64 , C07K14/745 , C07K1/16
Abstract: 본 발명은 쌀로부터 저장 단백질의 제거를 통한 플라스미노겐 액티베이터(plasminogen activator, t-PA)의 정제방법에 관한 것이다.
본 발명에 의한 t-PA 정제방법은 용이하며 경제적인 방법으로서, 식물체 발현 t-PA 정제방법의 확립을 통해 대량화 정제 시스템 기반기술의 제공할 수 있다.Abstract translation: 本发明涉及通过从水稻中除去贮藏蛋白来纯化纤溶酶原激活物（t-PA）的方法。 根据本发明，t-PA纯化方法是一种容易经济的方法，从而通过建立表达植物体的t-PA纯化方法提供了大规模的纯化系统基础技术。
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15.发明公开
公开(公告)号：KR1020080075421A
公开(公告)日：2008-08-18
申请号：KR1020070014589
申请日：2007-02-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A fusion protein of Escherichia coli enterotoxin protein and a neuraminidase epitope of Newcastle disease virus is provided to increase immunity against Newcastle disease virus in poultry, so that the protein is useful for prevention of Newcastle disease virus. A fusion protein of E. coli heat-labile enterotoxin subunit B and a neuraminidase epitope of Newcastle disease virus has the amino acid sequence of SEQ ID NO:1. A polynucleotide encoding the fusion protein has the nucleotide sequence of SEQ ID NO:2. An expression vector pLD-CtV-HN contains the polynucleotide of SEQ ID NO:2. A vaccine composition for preventing Newcastle disease virus contains the fusion protein as an effective ingredient. The immunity against Newcastle disease virus in poultry is increased by administering the fusion protein or a transgenic plant containing the expression vector pLD-CtV-HN into poultry.
Abstract translation: 提供大肠杆菌肠毒素蛋白的融合蛋白和新城疫病毒的神经氨酸酶表位，以增加对家禽新城疫病毒的免疫力，使得该蛋白质可用于预防新城疫病毒。 大肠杆菌热不稳定肠毒素亚基B和新城疫病毒的神经氨酸酶表位的融合蛋白具有SEQ ID NO：1的氨基酸序列。 编码融合蛋白的多核苷酸具有SEQ ID NO：2的核苷酸序列。 表达载体pLD-CtV-HN含有SEQ ID NO：2的多核苷酸。 用于预防新城疫病毒的疫苗组合物含有融合蛋白作为有效成分。 通过将融合蛋白或含有表达载体pLD-CtV-HN的转基因植物施用于家禽来增加家禽中新城疫病毒的免疫力。
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Patent Agency Ranking
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公开(公告)号：KR101508730B1
公开(公告)日：2015-04-08
申请号：KR1020130055851
申请日：2013-05-16
Applicant: 대한민국(농촌진흥청장)
IPC: C12N9/64 , C07K14/745 , C07K1/16
Abstract: 본발명은쌀로부터저장단백질의제거를통한플라스미노겐액티베이터(plasminogen activator, t-PA)의정제방법에관한것이다. 본발명에의한 t-PA 정제방법은용이하며경제적인방법으로서, 식물체발현 t-PA 정제방법의확립을통해대량화정제시스템기반기술의제공할수 있다.
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18.发明公开선인장바이러스엑스 피막단백질 유전자를 포함하는 알엔에이아이 운반체, 이를 이용하여 형질전환된 선인장 및 형질전환 선인장의 제조방법 无效含有CACATUS病毒X的基因编码CAPSID蛋白的RNAI载体，用变体转化的转基因小麦和转基因CACTUS的制备方法
公开(公告)号：KR1020100115088A
公开(公告)日：2010-10-27
申请号：KR1020090033624
申请日：2009-04-17
Applicant: 대한민국(농촌진흥청장) , 경기도농업기술원
Abstract: PURPOSE: An RNAi carrier containing cacatus virus X coat protein gene is provided to product transformed cactus having CVX resistance. CONSTITUTION: A cacatus virus X resistant plasmid(KACC 95089P) contains cacatus virus X coat protein gene of sequence number 1 in a forward direction and reverse direction. An Agrobacterium transformant is prepared using the cacatus virus X resistant plasmid. A method for producing the transformed cactus comprises: a preparing the plasmid to which the cacatus virus X coat protein gene; a step of transforming Agrobacterium using the plasmid; and a step of transforming cactus using the Agrobacterium.
Abstract translation: 目的：将含有cacatus病毒X外壳蛋白基因的RNAi载体提供给具有CVX抗性的产品转化仙人掌。 构成：Cacatus病毒X抗性质粒（KACC 95089P）在正向和反向方向上含有序列号1的cacatus病毒X外壳蛋白基因。 使用cacatus病毒X抗性质粒制备农杆菌转化体。 一种转化仙人掌的制备方法，其特征在于：制备所述cacatus病毒X外壳蛋白基因的质粒; 使用质粒转化土壤杆菌的步骤; 以及使用农杆菌转化仙人掌的步骤。
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19.发明授权
公开(公告)号：KR100874256B1
公开(公告)日：2008-12-16
申请号：KR1020070014589
申请日：2007-02-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A fusion protein of Escherichia coli enterotoxin protein and a neuraminidase epitope of Newcastle disease virus is provided to increase immunity against Newcastle disease virus in poultry, so that the protein is useful for prevention of Newcastle disease virus. A fusion protein of E. coli heat-labile enterotoxin subunit B and a neuraminidase epitope of Newcastle disease virus has the amino acid sequence of SEQ ID NO:1. A polynucleotide encoding the fusion protein has the nucleotide sequence of SEQ ID NO:2. An expression vector pLD-CtV-HN contains the polynucleotide of SEQ ID NO:2. A vaccine composition for preventing Newcastle disease virus contains the fusion protein as an effective ingredient. The immunity against Newcastle disease virus in poultry is increased by administering the fusion protein or a transgenic plant containing the expression vector pLD-CtV-HN into poultry.
Abstract translation: 提供了大肠杆菌肠毒素蛋白和新城疫病毒的神经氨酸酶表位的融合蛋白，以提高家禽对新城疫病毒的免疫力，从而使该蛋白可用于预防新城疫病毒。 大肠杆菌不耐热肠毒素亚基B和新城疫病毒的神经氨酸酶表位的融合蛋白具有SEQ ID NO：1的氨基酸序列。 编码融合蛋白的多核苷酸具有SEQ ID NO：2的核苷酸序列。 表达载体pLD-CtV-HN含有SEQ ID NO：2的多核苷酸。 用于预防新城疫病毒的疫苗组合物含有融合蛋白作为有效成分。 通过向家禽施用融合蛋白或含有表达载体pLD-CtV-HN的转基因植物来增加家禽对新城疫病毒的免疫力。
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20.发明授权수용성 유기용매에서 지속적 활성유지가 가능한 신규 β-글루코스 분해 효소 유전자 Bgls6과 이로부터 생산한 재조합 효소의 이용 有权使用能够连续活动的重组酶的保持新颖β-葡萄糖裂合酶基因Bgls6由其生产和在该水溶性有机溶剂
公开(公告)号：KR101791564B1
公开(公告)日：2017-11-01
申请号：KR1020150139655
申请日：2015-10-05
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은동애등에유충장내에존재하는난배양미생물로부터분리한유기용매에강한β-글루코시다아제유전자및 이의용도에관한것이다. 본발명은동애등에() 유충장내의난배양미생물로부터비환원성β-글리코시드말단의β-1,4-글리코시딕결합을분해하는신규효소유전자를분리하고, 이를대량으로발현하는신종균주를개발하여식물성고분자다당류를이용하는다양한산업적공정과정에서효율적으로적용할수 있다. 또한, 본발명에따른신규효소유전자의분해효과는산업공정과정외에식품발효산업과제지/직물산업, 알코올생산공정, 의약품제조및 식물다당류의당화작용등에이용될수 있다.
Abstract translation: 在本发明目前我在幼虫肠等dongae涉及强β-葡糖苷酶基因和在有机溶剂中使用从其培养的微生物被分离。 等。本发明dongae（幼虫肠）我分解分离新型酶基因的新菌株β-1,4-糖苷时的非还原末端，从培养的微生物β-糖苷，和Dick组合大量表达它 并且可以在使用植物高分子多糖的各种工业过程中有效地应用。 另外，也可以在除了用于食品发酵工业和纸/纺织工业，酒精生产过程中，药物制造工厂和多糖的糖化操作过程工业过程中使用根据本发明的新的基因的分解效果。
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