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    나비목 해충에 대한 살충활성이 향상된 신규의 바실러스 투린지엔시스 내독소단백질을 코딩하는 유전자 및 그 제조방법
    12.
    发明公开
    나비목 해충에 대한 살충활성이 향상된 신규의 바실러스 투린지엔시스 내독소단백질을 코딩하는 유전자 및 그 제조방법 有权
    新型巴豆酵母基因编码具有改善的左旋多巴素类药物的杀虫活性的晶体蛋白及其制备方法

    公开(公告)号：KR1020140117257A

    公开(公告)日：2014-10-07

    申请号：KR1020140004841

    申请日：2014-01-15

    Applicant: 서울대학교산학협력단

    Inventor： 제연호 ,  최재영 ,  김송은 ,  김재수

    IPC: C12N15/32 ,  C12N15/63 ,  C12N15/70 ,  C12Q1/68

    CPC classification number: C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162 ,  C12N15/11 ,  C12N15/63 ,  C12N15/70

    Abstract: The present invention relates to a novel bacillus thuringiensis endotoxic protein gene with improved insecticidal activities against lepidopteran insects and to a method for producing the same. More specifically, the present invention relates to a method for producing a bacillus thuringiensis endotoxic gene with strong insecticidal activities against lepidopteran insects such as Mamestra brassicae, Spodoptera litura, and Spodoptera exigua. The purpose of the present invention is achieved through the steps of: confirming a base sequence and a tertiary structure of a mod-cry1AC gene; producing a PIM-mod-cry1AC vector using the mod-cry1AC gene obtained from the previous step; producing a mutation-inducing primer using the mod-cry1AC as a mold; performing a polymerase chain reaction (PCR) using the mutation-inducing primer obtained from the previous step so as to produce novel BT endotoxic protein gene to be transformed in colon bacilli and confirming mutations; and confirming a base sequence and a tertiary structure of the novel BT endotoxic protein gene obtained from the previous step. Accordingly, in the present invention, agricultural products, which are not damaged by larvae of various lepidopteran insects, can be produced using a microorganism formulation including novel recombinant endotoxic protein gene Mut-N16.

    Abstract translation: 本发明涉及一种具有改善的对鳞翅目昆虫的杀虫活性的新型苏云金芽孢杆菌内毒素蛋白基因及其制备方法。 更具体地说，本发明涉及一种对鳞翅目昆虫（如Mamestra brassicae），斜纹夜蛾（Spodoptera litura）和斜纹夜蛾（Spodoptera exigua）具有强杀虫活性的苏云金芽孢杆菌内毒素基因的生产方法。 通过以下步骤实现本发明的目的：确认mod-cry1AC基因的碱基序列和三级结构; 使用从前一步获得的mod-cry1AC基因产生PIM-mod-cry1AC载体; 使用mod-cry1AC作为模具产生突变诱导引物; 使用从前一步骤获得的突变诱导引物进行聚合酶链反应（PCR），以产生要在大肠杆菌中转化的新的BT内毒素蛋白基因并确认突变; 并确认从前一步骤获得的新型BT内毒素蛋白质基因的碱基序列和三级结构。 因此，在本发明中，可以使用包含新型重组内毒素蛋白质基因Mut-N16的微生物制剂来制造未被各种鳞翅目昆虫的幼虫损伤的农产品。

    재조합바이러스 비이지박 (ｂＥａｓｙＢａｃ)과 이를 이용한 베큘로바이러스 발현벡터계
    13.
    发明授权
    재조합바이러스 비이지박 (ｂＥａｓｙＢａｃ)과 이를 이용한 베큘로바이러스 발현벡터계 有权
    非重组病毒不箔（bEasyBac），并使用这个bekyul病毒的表达载体系统

    公开(公告)号：KR101223577B1

    公开(公告)日：2013-01-17

    申请号：KR1020090039373

    申请日：2009-05-06

    Applicant: 서울대학교산학협력단

    Inventor： 제연호 ,  최재영 ,  노종열 ,  김양수 ,  진병래 ,  우수동

    IPC: C12N15/866 ,  C12N7/01

    Abstract: 본 발명은 베큘로바이러스 발현벡터계 (Baculovirus Expression Vector System)에 조기 발현 프로모터 (Promter), 치사유전자 (Lethal gene) 및 전이유전자 (Transposition gene)를 도입함으로써, 외래유전자를 발현하는 재조합바이러스 (recombinant virus)의 분리효율을 높이고 대량생산(high-throughput)이 가능하도록 제작한 재조합바이러스 비이지박 (bEasyBac)을 개시한다.
    베큘로바이러스 (baculovirus), 트랜스퍼벡터 (transfer vector), 바네이즈 (Barnase), 트랜스포지션 (Transposition), 재조합바이러스 (recombinant virus).

    Abstract translation: 在bekyul本发明的病毒表达载体系统（杆状病毒表达载体系统）早期表达启动子（Promter），致死基因（致死基因）和通过引入基因的（换位基因），重组病毒表达外源基因（重组病毒到过渡 （BEasyBac），其产生以增加重组病毒的分离效率并实现高通量。

    재조합 바이러스 에이씨이지-알비에스디브이1에스피 및 이를 이용한 베큘로바이러스 발현벡터 시스템
    18.
    发明公开
    재조합 바이러스 에이씨이지-알비에스디브이1에스피 및 이를 이용한 베큘로바이러스 발현벡터 시스템 有权
    重组病毒ACEASY-RBSDV1SP和BACULVVUSUS表达载体系统

    公开(公告)号：KR1020120089879A

    公开(公告)日：2012-08-16

    申请号：KR1020100127988

    申请日：2010-12-15

    Applicant: 서울대학교산학협력단

    Inventor： 제연호 ,  최재영 ,  노종열 ,  왕용

    IPC: C12N15/52 ,  C12N15/866

    Abstract: PURPOSE: A recombinant virus AcEasy-RBSDV1SP and a baculovirus expression vector system using the same are provided to diagnose rice black streaked dwarf virus. CONSTITUTION: An RNA-dependent RNA polymerase has a sequence of sequence number 1, isolated from rice black streaked dwarf virus. A transition vector, pDualBac-RBSDV1SP, contains a gene encoding RNA-dependent RNA polymerase. A recombinant virus is prepared by in vitro transposition of the transition vector to bEasyBac(KCTC 11499BP). A Baculovirus expression system for producing a large amount of RNA-dependent RNA polymerase of rice black streaked dwarf virus is prepared by transfection of recombinant virus AcEasy-RBSDV1SP(KCTC 11804BP) to insect cell line.

    Abstract translation: 目的：提供重组病毒AcEasy-RBSDV1SP和使用该病毒的杆状病毒表达载体系统来诊断米黑条纹矮化病毒。 构成：RNA依赖性RNA聚合酶具有从米黑条纹矮化病毒中分离的序列号1的序列。 转移载体pDualBac-RBSDV1SP包含编码RNA依赖性RNA聚合酶的基因。 通过将转移载体体外转座至bEasyBac（KCTC 11499BP）制备重组病毒。 通过将重组病毒AcEasy-RBSDV1SP（KCTC 11804BP）转染到昆虫细胞系来制备用于产生大量黑色条纹矮化病毒的RNA依赖性RNA聚合酶的杆状病毒表达系统。

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