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公开(公告)号：KR1020160073469A
公开(公告)日：2016-06-27
申请号：KR1020140181515
申请日：2014-12-16
Applicant: 서울대학교산학협력단
IPC: A01K67/027 , C12N15/85
CPC classification number: A01K67/027 , A01K67/0271 , C12N15/85
Abstract: 본원은목적유전자에결합하는 TALEN을코딩하는핵산을조류원시생식세포에도입하여상기목적유전자에돌연변이를유발하는단계, 상기돌연변이가유발된조류원시생식세포를수용체배아에주입하는단계; 및상기수용체배아를포함하는난을배양및 부화하여유전자변형체를생산하는단계를포함하는유전자적중에의한유전자편집조류의생산방법및 유전자편집조류를개시한다. 본원에따른방법은기존의상동재조합에기반을둔 방법과비교하여매우효율적으로다양한유전자를표적으로하여대량으로신품종조류의생산을가능하게한다.
Abstract translation: 公开了通过基因靶向生产基因编辑的家禽的方法和基因编辑的家禽。 该方法包括：通过将用于编码与靶基因组合的TALEN的核酸引入到原始生殖细胞中，引发靶基因突变的步骤; 将引起突变的禽类原始生殖细胞注射入受体胚胎的步骤; 以及通过培养和孵化包含受体胚的卵来生产基因组变体的步骤。 根据本发明，与基于常规同源重组的方法相比，各种基因可以有效地产生大量新品种。
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公开(公告)号：KR1020120057707A
公开(公告)日：2012-06-07
申请号：KR1020100080213
申请日：2010-08-19
Applicant: 서울대학교산학협력단
IPC: G01N33/574 , C12Q1/68 , C12N15/12 , G01N33/15
CPC classification number: G01N33/57449 , C12Q1/6886 , C12Q2600/158 , G01N33/15
Abstract: PURPOSE: A biomarker for diagnosing ovarian cancer and a cancer diagnosis kit are provided to simply and cheaply diagnose human ovarian cancer. CONSTITUTION: A kit for diagnosing cancer contains a binder which specifically binds to a nucleotide sequence encoding CLDN10(claudin 10) or MMP3(matix metalloproteinase 3) or a binder which is specific to CLDN10 or MMP3. The binder includes a probe, primer, antibody or aptamer. A pharmaceutical composition for preventing or treating cancer contains a material which suppresses CLDN10 or MMP3 expression as an active ingredient.
Abstract translation: 目的：诊断卵巢癌和癌症诊断试剂盒的生物标志物被简单且廉价地诊断人卵巢癌。 构成：用于诊断癌症的试剂盒含有特异性结合编码CLDN10（克隆蛋白10）或MMP3（matix金属蛋白酶3）的核苷酸序列或对CLDN10或MMP3特异的粘合剂的粘合剂。 粘合剂包括探针，引物，抗体或适体。 用于预防或治疗癌症的药物组合物含有抑制CLDN10或MMP3表达作为活性成分的材料。
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公开(公告)号：KR1020120054119A
公开(公告)日：2012-05-30
申请号：KR1020100115328
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: A01K67/027 , C12N15/867 , C12N15/65
CPC classification number: A01K67/027 , C12N15/65 , C12N15/867
Abstract: PURPOSE: The production of transgenic aves using sequences for germ cell-specific gene expression is provided to analyze the differentiation or development of the priordial germ cells of aves into germ cells. CONSTITUTION: A method for producing transgenic aves includes the following: the priordial germ cells of aves are transformed based on a genetic vector containing a DAZL promoter as a germ cell-specific promoter, a NANOG promoter as a stem cell-specific promoter, and nucleotide sequence which encodes protein of revelation purpose; the transformed priordial germ cells of the aves are injected into accept media; and the transformed product of the aves is obtained. The protein is operatively bonded to the promoters.
Abstract translation: 目的：提供使用生殖细胞特异性基因表达序列的转基因抗生素的生产，以分析生物细胞中二次生殖细胞的分化或发育。 构成：用于生产转基因鸟类的方法包括以下：基于含有作为生殖细胞特异性启动子的DAZL启动子，作为干细胞特异性启动子的NANOG启动子和核苷酸的核苷酸的遗传载体转化aves的无性繁殖细胞 编码启示目的蛋白的序列; 将转化的雄性生殖细胞注入接受培养基; 并获得aves的转化产物。 蛋白质可操作地结合到启动子上。
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公开(公告)号：KR1020110052390A
公开(公告)日：2011-05-18
申请号：KR1020090109390
申请日：2009-11-12
Applicant: 서울대학교산학협력단
IPC: C12N15/63 , C12N15/12 , C12N5/0735 , A01K67/027
Abstract: PURPOSE: A recombinant expression vector and a method for screening a material which promotes germ cell development are provided to study of animal germ cell development. CONSTITUTION: A gene expression regulatory sequence contains a DAZL(deleted in AZoospermia-like) promoter containing a nucleotide sequence of sequence number 1 or and 3'UTR(3'untranslated region) of sequence number 2. A germ cell-specific recombinant vector contains DAZL promoter and expression target-coding nucleotide sequence which is operatively linked to the promoter. The germ cells are primordial germ cells or gonocyte or prespermatogonia. A method for screening a material which promotes germ cell development comprises: a step of transforming an animal cell by a vector containing DAZL-encoding nucleotide sequence; a step of treating test material to the animal cells; and a step of analyzing DAZL gene expression in the animal cells.
Abstract translation: 目的：提供重组表达载体和筛选促进生殖细胞发育的物质的方法，用于研究动物生殖细胞发育。 构成：基因表达调控序列含有含有序列号1的核苷酸序列和序列号2的3'UTR（3'非翻译区）的核苷酸序列的DAZL（缺失精子样）启动子。生殖细胞特异性重组载体含有 DAZL启动子和与启动子有效连接的表达靶标编码核苷酸序列。 生殖细胞是原始生殖细胞或者是gonocyte或prepermatogonia。 用于筛选促进生殖细胞发育的材料的方法包括：通过含有编码DAZL的核苷酸序列的载体转化动物细胞的步骤; 向动物细胞处理试验材料的步骤; 以及在动物细胞中分析DAZL基因表达的步骤。
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公开(公告)号：KR101724805B1
公开(公告)日：2017-04-11
申请号：KR1020140181515
申请日：2014-12-16
Applicant: 서울대학교산학협력단
IPC: A01K67/027 , C12N15/85
Abstract: 본원은목적유전자에결합하는 TALEN을코딩하는핵산을조류원시생식세포에도입하여상기목적유전자에돌연변이를유발하는단계, 상기돌연변이가유발된조류원시생식세포를수용체배아에주입하는단계; 및상기수용체배아를포함하는난을배양및 부화하여유전자변형체를생산하는단계를포함하는유전자적중에의한유전자편집조류의생산방법및 유전자편집조류를개시한다. 본원에따른방법은기존의상동재조합에기반을둔 방법과비교하여매우효율적으로다양한유전자를표적으로하여대량으로신품종조류의생산을가능하게한다.
Abstract translation: 本文它包括注射步骤的步骤，joryuwon通过引入核酸期间joryuwon诱变与靶基因中的生殖细胞编码TALEN结合到目的基因的受体胚胎诱导突变的生殖细胞中; 培养和孵化含有受体胚胎的卵以产生转基因生物的步骤。 与基于常规同源重组的方法相比，根据本发明的方法可以通过以高效方式靶向各种基因而大量生产新的各种藻类。
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Patent Agency Ranking
公开(公告)号：KR101538089B1
公开(公告)日：2015-07-22
申请号：KR1020097005443
申请日：2006-12-20
Applicant: 서울대학교산학협력단
CPC classification number: C12N5/0696 , C12N2501/235 , C12N2502/03 , C12N2502/04 , C12N2502/13 , C12N2502/1305 , C12N2502/1311 , C12N2502/1317 , C12N2502/1323 , C12N2502/1329 , C12N2502/1335 , C12N2502/1341 , C12N2502/1347 , C12N2502/1352 , C12N2502/1358 , C12N2502/1364 , C12N2502/137 , C12N2502/1376 , C12N2502/1382 , C12N2502/1388 , C12N2502/1394
Abstract: 본발명은다음단계를포함하는배아줄기세포(ESC)-유사세포를제조하는방법에관한것이다: (a) 포유동물조직또는체액으로부터성체줄기세포를포함하는제1세포군을수득하는단계; (b) 상기단계 (a)에서의포유동물조직과상이한포유동물조직으로부터상기제1세포군과상이한제2체세포군을수득하는단계; (c) 상기제1세포군또는상기제2체세포군으로부터콜로니를형성하기위하여충분한시간동안상기제1세포군및 상기제2체세포군을배지에서공동배양하는단계; 및 (d) ESC-유사세포를제조하기위하여충분한시간동안상기콜로니의세포를계대배양하는단계.
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公开(公告)号：KR101232850B1
公开(公告)日：2013-02-22
申请号：KR1020100115395
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
Abstract: 본 발명은 서열번호 제1서열 내지 제5서열로 구성된 군으로부터 선택되는 뉴클레오타이드 서열에 특이적으로 결합하는 결합제를 포함하는 생식관의 발생 또는 분화 분석용 키트 및 이를 이용한 스크리닝 방법에 관한 것이다. 본 발명자들은 닭의 난관에 합성 에스트로겐, 바람직하게는 디에틸스틸베스트롤(diethylstilbestrol, DES)을 처리하여 실시한 전사체 프로파일(transcriptional profiling) 분석을 통해 생식관(바람직하게는, 난관) 발생 또는 분화-특이적 신규한 바이오마커인
CCRN4L (carbon catabolite repression 4-like),
NTM (neurotrimin),
HAS2 (hyaluronan synthase 2),
NELF (nasal embryonic LHRH factor) 및
FAM26F (family with sequence similarity 26, member F) 유전자를 동정하였다. 따라서, 본 발명의 마커 및 방법은 닭의 난관 발생에 필요한 유전자 분화 및 발생을 동정하기 위한 난관의 유전적 조절에 대한 새로운 시각을 제공한다.-
公开(公告)号：KR1020120054151A
公开(公告)日：2012-05-30
申请号：KR1020100115395
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
CPC classification number: G01N33/5302 , C12Q1/6837 , C12Q1/6844 , G01N33/74
Abstract: PURPOSE: A kit for analyzing development or differentiation of gonaduct is provided to identify gene differentiation and development. CONSTITUTION: A kit for analyzing development or differentiation of gonaduct contains a binder which specifically binds to a nucleotide sequence selected from sequence numbers 1-5. The expression of the nucleotide sequence is increases in the gonaduct by a material with estrogen activity. The material is synthetic estrogen or biogenic estrogen. The synthetic estrogen is diethyl stilbestrol. The binder is a probe, primer, antibody, or aptamer.
Abstract translation: 目的：提供分析发育或分化导管的试剂盒，以鉴别基因分化和发育。 构成：用于分析导管的发育或分化的试剂盒含有特异性结合选自序列号1-5的核苷酸序列的粘合剂。 核苷酸序列的表达是雌激素活性物质在导管中增加。 该物质是合成雌激素或生物雌激素。 合成雌激素是二苯乙烯雌酚。 粘合剂是探针，引物，抗体或适体。
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公开(公告)号：KR1020120014404A
公开(公告)日：2012-02-17
申请号：KR1020100076439
申请日：2010-08-09
Applicant: 서울대학교산학협력단 , 한미약품 주식회사
IPC: A01K67/027 , C12N15/86 , C12N15/12
CPC classification number: A01K67/0275 , A01K2227/30 , A01K2267/0368 , C12N15/8509 , C12N15/86
Abstract: PURPOSE: A method for producing protein is provided to produce recombinant human interleukin 1 receptor antagonist protein. CONSTITUTION: A method for producing protein is as follows. The method uses a recombination vector which peculiarly expresses recombinant human interleukin 1 receptor antagonist protein as oviduct. The recombination vector comprises an avalbumin promoter and recombinant human interleukin 1 receptor antagonist protein-coding nucleotide sequence that is automatically connected to the promoter.
Abstract translation: 目的：提供一种生产蛋白质的方法，以产生重组人白细胞介素1受体拮抗蛋白。 构成：生产蛋白质的方法如下。 该方法使用特异性表达重组人白细胞介素1受体拮抗蛋白作为输卵管的重组载体。 重组载体包含自动连接到启动子的阿瓦美特启动子和重组人白细胞介素1受体拮抗剂蛋白编码核苷酸序列。
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公开(公告)号：KR1020090074018A
公开(公告)日：2009-07-03
申请号：KR1020097005443
申请日：2006-12-20
Applicant: 서울대학교산학협력단
CPC classification number: C12N5/0696 , C12N2501/235 , C12N2502/03 , C12N2502/04 , C12N2502/13 , C12N2502/1305 , C12N2502/1311 , C12N2502/1317 , C12N2502/1323 , C12N2502/1329 , C12N2502/1335 , C12N2502/1341 , C12N2502/1347 , C12N2502/1352 , C12N2502/1358 , C12N2502/1364 , C12N2502/137 , C12N2502/1376 , C12N2502/1382 , C12N2502/1388 , C12N2502/1394
Abstract: 본발명은다음단계를포함하는배아줄기세포(ESC)-유사세포를제조하는방법에관한것이다: (a) 포유동물조직또는체액으로부터성체줄기세포를포함하는제1세포군을수득하는단계; (b) 상기단계 (a)에서의포유동물조직과상이한포유동물조직으로부터상기제1세포군과상이한제2체세포군을수득하는단계; (c) 상기제1세포군또는상기제2체세포군으로부터콜로니를형성하기위하여충분한시간동안상기제1세포군및 상기제2체세포군을배지에서공동배양하는단계; 및 (d) ESC-유사세포를제조하기위하여충분한시간동안상기콜로니의세포를계대배양하는단계.
Abstract translation: 提供胚胎干细胞（ESC）样细胞以从哺乳动物细胞产生ESC样细胞，而不用生殖细胞的操纵。 一种用于产生胚胎干细胞（ESC）样细胞的方法包括：从哺乳动物组织或体液获得含有成体干细胞的第一细胞群的步骤; 从其他哺乳动物的组织获得第二体细胞群的步骤; 共培养第一细胞群和第二体细胞群以从细胞群形成菌落的步骤; 以及将菌落细胞分培养足够时间以产生ESC样细胞的步骤。 第一个细胞组是含有两种或多种细胞类型的异质群体。 体细胞群是含有一种体细胞类型的同源群体。
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