公开(公告)号：KR100509803B1

公开(公告)日：2005-08-23

申请号：KR1020020064846

申请日：2002-10-23

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  이진아

IPC: A61K38/16

Abstract: 본 발명은 세포 형태 특이적인 방법으로 시냅스 가소성을 저해시킬 수 있는 글루타민 반복단위를 35개 이상 가지는 단백질을 포함하는 시냅스 가소성 저해제에 관한 것이다. 본 발명의 시냅스 가소성 저해제는 시냅스 가소성중 감각-운동 시냅스의 기본적인 기능이나 단기 기억 촉진에는 영향을 미치지 않으나 장기 기억 촉진을 손상시키므로 헌팅톤 병과 같은 신경퇴행성 질병에 대한 병리학적 메커니즘 연구에 유용하게 이용될 수 있다.

公开(公告)号：KR100486792B1

公开(公告)日：2005-04-29

申请号：KR1020020051210

申请日：2002-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김정연

IPC: C07K14/475

Abstract: 본 발명은 신경돌기 성장촉진용 ＭＤＧＦ 및 헤모림프 유래 단백질 분획물에 관한 것이다. 특히 본 발명은 신경돌기의 성장을 촉진시켜 신경세포 재생 또는 기억력 증가를 위한 용도로 사용할 수 있는 MDGF 및 헤모림프 유래 단백질 분획물에 관한 것이다.

公开(公告)号：KR100471604B1

公开(公告)日：2005-03-10

申请号：KR1020020051212

申请日：2002-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김형규

IPC: C12N15/12

Abstract: 본 발명은 참군소에서 분리한 ApC/EBP 및 ApC/EBP 5'-플랭킹 부위에 관한 것이다. 특히 본 발명은 장기 기억에 작용하는 ApC/EBP 및 이의 발현을 조절하는 ApC/EBP 5'-플랭킹 부위에 관한 것이다.

公开(公告)号：KR1020040020225A

公开(公告)日：2004-03-09

申请号：KR1020020051753

申请日：2002-08-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김수동

IPC: A61K31/327

Abstract: PURPOSE: A membrane potential changer of an aplysia neuron containing hydrogen peroxide and a cell death-inducing composition of the aplysia neuron containing the membrane potential changer are provided. Use of the composition induces a change in membrane potential and cell death of the aplysia neuron and thus can provide a model useful for the study of memory and learning. CONSTITUTION: The membrane potential changer of an aplysia neuron contains hydrogen peroxide in the range concentration of 0.01 to 10mM. The cell death-inducing composition of the aplysia neuron contains the membrane potential changer of the aplysia neuron. A change in membrane potential is hyperpolarized in the early time after hydrogen peroxide treatment and then depolarized after then. The aplysia is an Aplysia kurodai captured in a costal area of The East Sea of Korea.

Abstract translation: 目的：提供含有过氧化氢的蛋白质神经元的膜电位变化器和含有膜电位变化器的含有神经元的细胞死亡诱导组合物。 组合物的使用会导致膜电位的变化，并且可以提供一种可用于研究记忆和学习的模型。 构成：aplysia神经元的膜电位变化器含有浓度为0.01〜10mM的过氧化氢。 aplysia神经元的细胞死亡诱导组合物含有aplysia神经元的膜电位变化。 膜电位的变化在过氧化氢处理后的早期被超极化，然后在此之后去极化。 大赦国际是在韩国东海沿海地区捕获的阿派利亚kurodai。

公开(公告)号：KR100782140B1

公开(公告)日：2007-12-06

申请号：KR1020060053476

申请日：2006-06-14

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김형

IPC: C12N15/10 ,  C12N15/09 ,  C12N15/63

Abstract: An ApLLP transcription factor derived from Aplysia kurodai is provided to be used as a pharmaceutical composition for improving memory and a screening system for screening an active material useful for the long term memory formation. A primer pair for amplifying a gene of Aplysia kurodai includes sequences described as SEQ ID : NOs. 3 and 4. A method for selecting or searching candidate materials promoting the long term memory formation comprises the steps of: (a) injecting a group of candidate materials effective for the long term memory formation into neurons of Aplysia kurodai and culturing it; and (b) measuring the expression amount of a transcriptional factor described as SEQ ID : NO. 1.

Abstract translation: 提供源自黑腹果蝇的ApLLP转录因子用作改善记忆的药物组合物和筛选用于长期记忆形成的活性物质的筛选系统。 用于扩增黑腹果蝇基因的引物对包括SEQ ID NO：所示的序列。 用于选择或搜索促进长期记忆形成的候选材料的方法包括以下步骤：（a）将有效长期记忆形成的一组候选材料注入到黑腹苷神经元中并进行培养; 和（b）测量如SEQ ID NO：1所述的转录因子的表达量。 1。

公开(公告)号：KR1020040084559A

公开(公告)日：2004-10-06

申请号：KR1020030019712

申请日：2003-03-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  허민정

IPC: C07K14/47

CPC classification number: C07K14/4705

Abstract: PURPOSE: Protein msec7 with novel function is provided, which regulates neuritogenesis and synaptic transmission in neuron of Aplysia kurodai, and relates to membrane traffic of the neuron cell and modulation of actin cytoskeleton. Therefore, the protein msec7 is useful in the study on synaptic plasticity and gene transfer. CONSTITUTION: The protein msec7 regulates neuritogenesis and synaptic transmission by GEF(GDP/GTP exchange factor) activity dependent method, wherein the protein msec7 induces neuritogenesis by modulation of actin cytoskeleton; the protein msec7 increases varicosity by GEF(GDP/GTP exchange factor) activity dependent method; the protein msec7 comprises msec7-1 and msec7-2; and the protein msec7-1 is encoded by the nucleotide sequence of SEQ ID NO:1 or SEQ ID NO2, and the protein msec7-2 is encoded by the nucleotide sequence of SEQ ID NO:3.

Abstract translation: 目的：提供具有新功能的蛋白质msec7，其调节神经细胞神经元的神经发生和突触传递，涉及神经元细胞的膜交换和肌动蛋白细胞骨架的调节。 因此，蛋白质msec7在突触可塑性和基因转移研究中是有用的。 构成：蛋白质msec7通过GEF（GDP / GTP交换因子）活性依赖性方法调节神经发生和突触传递，其中蛋白质msec7通过调节肌动蛋白细胞骨架诱导神经发生; 蛋白质msec7通过GEF（GDP / GTP交换因子）活性依赖性方法增加静脉曲张; 蛋白质msec7包括msec7-1和msec7-2; 并且蛋白质msec7-1由SEQ ID NO：1或SEQ ID NO 2的核苷酸序列编码，并且蛋白质msec7-2由SEQ ID NO：3的核苷酸序列编码。

公开(公告)号：KR1020040084558A

公开(公告)日：2004-10-06

申请号：KR1020030019711

申请日：2003-03-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김형

IPC: C07K14/47

CPC classification number: C07K14/43504

Abstract: PURPOSE: A protein having a nucleus localization signal sequence is provided. The protein relates to nucleus or nucleolus targeting. CONSTITUTION: The protein has a nucleus localization signal sequence relating to nucleus or nucleolus targeting of Aplysia kurodai, wherein nucleus or nucleolus is encoded by the nucleotide sequence set forth in SEQ ID NO:1, and is present in the site encoded by the 85th to 142th and 355th to 445th nucleotides. The protein has a nucleus localization signal sequence which relates to nucleus or nucleolus targeting of human, wherein nucleus or nucleolus is encoded by the amino acid sequence set forth in SEQ ID NO:5, and is present in the site encoded by the 103th to 119th amino acids. The protein has a nucleus localization signal sequence relating to nucleus or nucleolus of mouse, wherein nucleus or nucleolus is encoded by the nucleotide sequence set forth in SEQ ID NO:6, and is present in the site encoded by the 47th to 107th and 364th to 413th nucleotides. Primers for producing the nucleus localization signal sequence has the nucleotide sequences of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:12 or SEQ ID NO:14.

Abstract translation: 目的：提供具有核定位信号序列的蛋白质。 该蛋白质涉及核或核仁靶向。 构成：该蛋白质具有涉及黑腹果蝇的核或核仁靶的核定位信号序列，其中核或核仁由SEQ ID NO：1所示的核苷酸序列编码，并且存在于由第85位至 第142和第355至445个核苷酸。 该蛋白质具有涉及人的核或核仁靶向的核定位信号序列，其中核或核仁由SEQ ID NO：5所示的氨基酸序列编码，并存在于第103至第119位编码的位点 氨基酸。 该蛋白质具有与小鼠核或核仁相关的核定位信号序列，其中核或核仁由SEQ ID NO：6所示的核苷酸序列编码，并存在于第47至107和364位编码的位点 第413位核苷酸。 用于产生核定位信号序列的引物具有SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：12或SEQ ID NO：14的核苷酸序列。

公开(公告)号：KR1020040020226A

公开(公告)日：2004-03-09

申请号：KR1020020051754

申请日：2002-08-30

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  장덕진

IPC: A61K31/327

Abstract: PURPOSE: A K ion channel mediator of a sensory neuron of aplysia containing hydrogen peroxide is provided. A sensory neuron system of aplysia using the K ion channel mediator can be provided as a model for the study of memory and learning. CONSTITUTION: The mediator for K ion channel of a sensory neuron of aplysia contains hydrogen peroxide. In the K ion channel, a hydrogen-induced depolarization is inhibited by tetraethylammonium(TEA). The concentration of hydrogen peroxide in the range of 0.01 to 10mK induces hyperpolarization within 1hr and contributes to the increase of membrane conductance. The aplysia is an Aplysia kurodai captured in a costal area of The East Sea of Korea.

Abstract translation: 目的：提供含有过氧化氢的含有aplysia的感觉神经元的K离子通道介体。 可以提供使用K离子通道介质的感觉神经元系统，作为记忆和学习研究的模型。 构成：aplysia感觉神经元K离子通道的介质含有过氧化氢。 在K离子通道中，氢诱导的去极化被四乙基铵（TEA）抑制。 0.01〜10mK范围内的过氧化氢浓度在1小时内引起超极化，有助于膜电导的增加。 大赦国际是在韩国东海沿海地区捕获的阿派利亚kurodai。

公开(公告)号：KR1020040019614A

公开(公告)日：2004-03-06

申请号：KR1020020051212

申请日：2002-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김형규

IPC: C12N15/12

CPC classification number: C07K14/43504

Abstract: PURPOSE: An Aplysia CCAAT enhancer-binding protein(ApC/EBP) isolated from Aplysia kurodai and a 5'-flanking region are provided, thereby increasing expression of ApC/EBP related to long-term memory and consequently improving the long-term memory. CONSTITUTION: A polynucleotide encoding Aplysia CCAAT enhancer-binding protein(ApC/EBP) comprises the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 8, wherein the polynucleotide has the nucleotide sequence set forth in SEQ ID NO: 7. A C/EBP(CCAAT-enhancer binding protein long form) polynucleotide comprises the amino acid sequence set forth in SEQ ID NO: 29. A C/EBP(CCAAT-enhancer binding protein short form) polynucleotide comprises the amino acid sequence set forth in SEQ ID NO: 30. A C/EBP 5-flanking region polynucleotide comprises the amino acid sequence set forth in SEQ ID NO: 28. A polynucleotide encoding Aplysia ribosome protein 4(ApS4) comprises the nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO: 10, wherein the polynucleotide has the nucleotide sequence set forth in SEQ ID NO: 9. A vector containing the C/EBP 5-flanking region and a foreign gene is provided, wherein the vector expresses the foreign gene when 5-HT is present; the C/EBP 5-flanking region is selected from the nucleotide sequences set forth in SEQ ID NO: 24, SEQ ID NO: 25 and SEQ ID NO: 28; and the foreign gene is selected from GFP gene, luciferase gene and LacZ gene.

Abstract translation: 目的：提供从阿维链霉菌和5'-侧翼区分离的Aplysia CCAAT增强子结合蛋白（ApC / EBP），从而增加与长期记忆相关的ApC / EBP的表达，从而改善长期记忆。 构成：编码Aplysia CCAAT增强子结合蛋白（ApC / EBP）的多核苷酸包含编码具有SEQ ID NO：8所示氨基酸序列的多肽的核苷酸序列，其中所述多核苷酸具有SEQ ID NO ：AC / EBP（CCAAT-增强子结合蛋白长形式）多核苷酸包含SEQ ID NO：29所示的氨基酸序列.AC / EBP（CCAAT-增强子结合蛋白短型）多核苷酸包含阐述的氨基酸序列 AC / EBP 5-侧翼区多核苷酸包含SEQ ID NO：28所示的氨基酸序列。编码Aplysia核糖体蛋白4（ApS4）的多核苷酸包含编码具有氨基酸的多肽的核苷酸序列 提供了SEQ ID NO：10所示的序列，其中所述多核苷酸具有SEQ ID NO：9所示的核苷酸序列。提供含有C / EBP 5-侧翼区和外源基因的载体，其中载体e 当5-HT存在时，表达外源基因; C / EBP 5-侧翼区选自SEQ ID NO：24，SEQ ID NO：25和SEQ ID NO：28所示的核苷酸序列; 外源基因选自GFP基因，荧光素酶基因和LacZ基因。

公开(公告)号：KR1020040019612A

公开(公告)日：2004-03-06

申请号：KR1020020051210

申请日：2002-08-28

Applicant: 재단법인서울대학교산학협력재단

Inventor： 강봉균 ,  김정연

IPC: C07K14/475

CPC classification number: C07K14/475 ,  C07K1/30

Abstract: PURPOSE: A mollusk derived growth factor(MDGF) for stimulating neurite outgrowth and a protein fraction isolated from Aplysia hemolymph are provided, thereby reviving neurite and increasing memory of a person. CONSTITUTION: A mollusk derived growth factor(MDGF) for stimulating neurite outgrowth is derived from Aplysia californica. A purification method of protein fraction for stimulating neurite outgrowth from hemolymph comprises the steps of: (a) extracting Aplysia hemolymph; slowly adding 40 to 60% of ammonium sulfate into the hemolymph to dissolve the hemolymph; (c) centrifuging the hemolymph-dissolved solution to obtain a precipitation; and (d) dialysis of the precipitation to remove ammonium sulfate.

Abstract translation: 目的：提供用于刺激神经突生长的软体动物来源的生长因子（MDGF）和从Aplysia血淋巴分离的蛋白质级分，从而恢复神经突并增加人的记忆力。 构成：用于刺激神经突生长的软体动物来源的生长因子（MDGF）源自加利福尼亚大蠊（Aplysia californica）。 蛋白质级分用于刺激血淋巴神经突生长的纯化方法包括以下步骤：（a）提取Aplysia血淋巴; 慢慢地将40〜60％的硫酸铵加入血淋巴溶解血淋巴; （c）离心溶血溶液以获得沉淀; 和（d）沉淀透析以除去硫酸铵。