公开(公告)号：KR101433049B1

公开(公告)日：2014-10-14

申请号：KR1020130085609

申请日：2013-07-19

Applicant: 충남대학교산학협력단

Inventor： 김민규 ,  이지혜 ,  김근중 ,  김은영 ,  박강선 ,  한길우 ,  이경본

IPC: C12N5/10 ,  C12N15/12 ,  A01K67/027 ,  G01N33/15

Abstract: The present invention relates to a method for manufacturing an animal model in which Parkinson′s disease related to the expression of DJ-1 gene is induced, an animal model manufactured by the same, and a method for judging the effect of a treatment candidate substance or a medicine of Parkinson′s disease by using the animal model. According to the present invention, in a research of a research model for treating Parkinson′s disease and a medicine for treating Parkinson′s disease, not only is difference between a normal tissue and a Parkinson′s disease expressing tissue biochemically compared, but also substances applicable as a medicine for Parkinson′s disease is detected by using the animal model to quantitively compare and judge the effect.

Abstract translation: 本发明涉及一种制造其中诱导与DJ-1基因的表达相关的帕金森病的动物模型的制造方法，以及由该方法制造的动物模型以及用于判断治疗候选物质的作用的方法 或使用动物模型的帕金森病药物。 根据本发明，在用于治疗帕金森病的研究模型和治疗帕金森病的药物的研究中，不仅正常组织与表达组织的帕金森病之间的差异在生物化学上比较，而且 通过使用动物模型量化比较和判断效果，可以检测到适用于帕金森病药物的物质。

公开(公告)号：KR1020140107804A

公开(公告)日：2014-09-05

申请号：KR1020130021817

申请日：2013-02-28

Applicant: 충남대학교산학협력단

Inventor： 김민규 ,  김근중 ,  이지혜 ,  김은영 ,  김마리아 ,  김종상 ,  김홍주 ,  임재삼 ,  백준종 ,  이정웅

IPC: C12N15/65 ,  C12N15/62 ,  C12N5/10 ,  A01K67/027

Abstract: The present invention, as a method for mass-producing medicine to treat rheumatoid arthritis, relates to a method for producing medicine by expressing gene from mammary gland of a transformed animal using expression vector including base sequence specifically expressed and controlled in mammary gland of a pig. More specifically, the present invention is production of a pig, a transformed animal through somatic cell clone after making recombinant protein to mass-produce TNF-R fusion protein and then transfecting the recombinant protein into a somatic cell of a pig.

Abstract translation: 作为用于大规模生产药物治疗类风湿性关节炎的方法本发明涉及通过使用包括在猪的乳腺中特异表达和控制的碱基序列的表达载体来表达转化动物的乳腺基因来生产药物的方法 。 更具体地说，本发明是在制备重组蛋白质大规模生产TNF-R融合蛋白后，通过体细胞克隆生产转化动物，然后将重组蛋白转染到猪的体细胞中。

公开(公告)号：KR101390536B1

公开(公告)日：2014-05-07

申请号：KR1020130033861

申请日：2013-03-28

Applicant: 충남대학교산학협력단 ,  전라북도 임실군(임실군 농업기술센터장)

Inventor： 김민규 ,  박연배 ,  한길우 ,  이준헌 ,  이경본 ,  장옥 ,  이기곤 ,  정일윤 ,  이성구

IPC: A01K67/027 ,  C12Q1/68 ,  A61D19/04

CPC classification number: A01K67/0275 ,  A61D19/04 ,  C12Q1/683 ,  C12Q2600/156

Abstract: The present invention provides a model and technology which selects a high capacity impotent cow having excellent heredity ability and collects eggs in vivo so as to enable mass propagation of excellent fertilized eggs by biotechnology technique, embryo transfer technology, etc., thereby acquiring, preserving, and maintaining outstanding genetic resources of hanwoo (Korean native cattle) in farm, and moreover, a high-quality meat production system is prepared so as to increase recessed hanwoo breeding farms and ensure competitiveness of hanwoo which is differentiated to imported meat.

Abstract translation: 本发明提供一种模型和技术，其选择具有优异遗传能力的高容量无能奶牛，并在体内收集鸡蛋，以便通过生物技术，胚胎移植技术等能够大量繁殖优质受精卵，从而获得，保存， 并在农场保持韩国优秀遗传资源，并制定优质的肉类生产系统，以增加农业部门的繁荣农业，确保与进口肉类不同的hanwoo的竞争力。

公开(公告)号：KR1020110022759A

公开(公告)日：2011-03-08

申请号：KR1020090080159

申请日：2009-08-28

Applicant: 충남대학교산학협력단

Inventor： 김민규 ,  최선아 ,  이지혜 ,  김근중

IPC: C12N5/07 ,  C12N5/02

Abstract: PURPOSE: A cell therapeutic agent containing amniotic fluid-derived mesenchymal stem cells is provided to ensure superior differentiation ability. CONSTITUTION: A method for preparing canine amniotic fluid-derived mesenchymal stem cells comprises: a step of collecting amniotic fluid from a pregnant dog using a syringe; a step of centrifuging amniotic fluid to collect pellet; a step of culturing the pellet in a medium containing bFGF(basic fibroblast growth factor) and EGF(epidermal growth factor) and collecting cultured cells; and a step of isolating and purifying the stem cells. The method for further comprises a step of inducing cell adhesion. The medium contains 0.5-20 ng of bFGF, 1-40 ng of EGF, 1-40% FBS(fetal bovine serum), and 0.5-4% penicillin-streptomycin. A composition for controlling differentiation of mesenchymal stem cells to adipocytes contains FBS, IBMX, dexamethasone, indomethacin, and insulin as active ingredient.

Abstract translation: 目的：提供含有羊水间充质干细胞的细胞治疗剂，以确保优异的分化能力。 构成：用于制备犬羊水间充质干细胞的方法包括：使用注射器从怀孕犬采集羊水的步骤; 离心羊水收集颗粒的步骤; 在含有bFGF（碱性成纤维细胞生长因子）和EGF（表皮生长因子）的培养基中培养该颗粒并收集培养的细胞的步骤; 以及分离和纯化干细胞的步骤。 该方法还包括诱导细胞粘附的步骤。 培养基含有0.5-20ng的bFGF，1-40ng的EGF，1-40％的FBS（胎牛血清）和0.5-4％的青霉素 - 链霉素。 用于控制间充质干细胞分化为脂肪细胞的组合物含有FBS，IBMX，地塞米松，吲哚美辛和胰岛素作为活性成分。

公开(公告)号：KR1020170039936A

公开(公告)日：2017-04-12

申请号：KR1020150139144

申请日：2015-10-02

Applicant: 충남대학교산학협력단

Inventor： 김영아 ,  김민규 ,  윤치선 ,  이수현 ,  조성현

IPC: A23L13/60

Abstract: 본발명은말토덱스트린, 콜라겐, 소금, 시즈닝, 인산염, 아스코르브산, 청양고추및 자몽추출물을봉지에담고밀봉한믹스봉지, 깔때기, 소시지케이싱및 설명서를포함하는소시지제조용패키지및 이의제조방법과 (a) 다진고기에물, 말토덱스트린, 콜라겐, 소금, 시즈닝, 인산염, 아스코르브산, 청양고추및 자몽추출물을혼합한후 숙성시키는단계; 및 (b) 상기 (a)단계의숙성시킨혼합물을깔때기에채우고, 깔때기를눌러혼합물을소시지케이싱에충진한후 가열하는단계를포함하여제조하는것을특징으로하는소시지의제조방법및 상기방법으로제조된소시지에관한것이다.

Abstract translation: 本发明的麦芽糊精，胶原蛋白，盐，调味品，磷酸，抗坏血酸，Chungyang辣椒和混合含有袋，漏斗，香肠生产含有一肠衣和文档及其制备方法和封装的葡萄柚提取物密封的袋（一个 ）老化的水，麦芽糖糊精，胶原蛋白，盐，调味品，磷酸盐，抗坏血酸，晴扬胡椒和肉末的柚子提取物混合后的步骤; 及（b），其中（a）制备由步骤老化的混合物以填充液漏斗中，然后通过按压液漏斗中的混合物填充到香肠的肠衣的制造方法，其特征在于，用于产生包括加热步骤，并且所述方法 它涉及到一个香肠。

公开(公告)号：KR20210015057A

公开(公告)日：2021-02-10

申请号：KR20190093334

申请日：2019-07-31

Applicant: 주식회사 엠케이바이오텍 ,  충남대학교산학협력단

Inventor： 김민규 ,  박강선 ,  이지혜 ,  김은영 ,  길태영

IPC: A61K36/258 ,  A23K10/30 ,  A23K50/40 ,  A61P15/08

Abstract: 본발명은흑삼분말을포함하는개과동물의정자운동성증가용조성물에관한것으로, 개과동물이섭취시, 정자운동성이개선될뿐 아니라, 남성호르몬에영향을미치지않는것으로번식효율을높일수 있다.

公开(公告)号：KR102066218B1

公开(公告)日：2020-01-14

申请号：KR1020190045801

申请日：2019-04-19

Applicant: 주식회사 엠케이바이오텍 ,  충남대학교산학협력단

Inventor： 김민규 ,  남윤석 ,  박강선 ,  지국빈 ,  이지혜

IPC: A01N1/02 ,  C12N5/075

公开(公告)号：KR101943158B1

公开(公告)日：2019-01-28

申请号：KR1020160117142

申请日：2016-09-12

Applicant: 충남대학교산학협력단

Inventor： 김민규 ,  이지혜 ,  김근중 ,  김은영 ,  박강선 ,  한길우 ,  이보명

IPC: C12N15/873 ,  A01K67/027 ,  A61D19/04

公开(公告)号：KR101512380B1

公开(公告)日：2015-04-16

申请号：KR1020140008831

申请日：2014-01-24

Applicant: 충남대학교산학협력단

Inventor： 홍순택 ,  김민규 ,  이의석 ,  박강선 ,  김산성

IPC: A61D19/02

CPC classification number: A61D19/02 ,  A01K67/02

Abstract: 본발명은인공수정용캡슐화가축정자비드제조방법에관한것으로, 대량생산체계에적합한유화(emulsion) 기술을이용하여가축정자를비드(bead) 형태로캡슐화하여안정적으로우수한정자를보급및 대량생산을할 수있음을확인하였으며, 상기가축정자비드는방출(release) 시충분한회수율을보유하고있는것을확인함으로써, 인공수정용캡슐화가축정자비드제조방법으로유용하게사용될수 있다.

Abstract translation: 本发明涉及一种用于体外受精的包囊牛精子制备方法，确认本发明采用适合大规模生产结构的乳液技术，并以稳定的方式将牛精子包埋在珠粒中，从而使分布和 批量生产优质精子，同时也证实，与牛精子包裹的珠粒在释放时显示出良好的恢复率。 因此，本发明可以有效地用作将精子包埋在用于人工授精的珠粒中的方法。