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公开(公告)号：KR101160794B1
公开(公告)日：2012-06-27
申请号：KR1020110138839
申请日：2011-12-21
Applicant: 충청남도 , 충남대학교산학협력단
CPC classification number: C12Q1/6827 , C12Q1/6888 , C12Q2531/113 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A genetic marker for identifying fatty acid composition of Hanwoo(Korean beef) is provided to easily select Hanwoo with high quality. CONSTITUTION: A method for selecting Hanwoo with high unsaturated fatty acid content comprises: a step of isolating genome DNA sample from a Hanwoo; a step of amplifying PCR of a genetic marker of sequence number 1 containing SNP at 21th base using a primer; a step of cleaving a PCR product with restriction enzyme; a step of performing electrophoresis to identify fragment number and size; and a step of detecting genotype of 21th base. The primer is a primer of sequence numbers 3 and 4. The restriction enzyme is Rsal.
Abstract translation: 目的：用于确定Hanwoo（韩国牛肉）脂肪酸组成的遗传标记，可轻松选择高质量的Hanwoo。 构成：选择具有高不饱和脂肪酸含量的Hanwoo的方法包括：从Hanwoo分离基因组DNA样品的步骤; 使用引物扩增第21位含有SNP的序列号1的遗传标记物的PCR的步骤; 用限制酶切割PCR产物的步骤; 进行电泳以识别片段数目和大小的步骤; 检测第21位基因型的步骤。 引物是序列号3和4的引物。限制酶是Rsal。
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公开(公告)号：KR101997453B1
公开(公告)日：2019-07-09
申请号：KR1020180009264
申请日：2018-01-25
Applicant: 충남대학교산학협력단
IPC: C12Q1/6883
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公开(公告)号：KR1020150050499A
公开(公告)日：2015-05-08
申请号：KR1020140149679
申请日：2014-10-30
Applicant: 충남대학교산학협력단
CPC classification number: C12Q1/6827 , C12Q1/6876 , C12Q2600/124 , C12Q2600/156
Abstract: 본발명은한국산재래닭의정강이색깔에관여하는 DNA 표지인자개발에관한것으로서, 한국산재래닭의정강이색깔에대한유전능력을조기에예측하고식별할수 있는 DNA 표지인자를개발하여한국산재래닭의경제형질개선또는육종개량프로그램에이용하는데 있다. 본발명의 DNA 표지인자를한국산재래닭의정강이색을확인할수 있는한국산재래닭의조기선발도구로활용하는경우, 소비자가한국산재래닭에요구하는정강이색, 구체적으로밝은노란색에가까운노란색의정강이색을갖는수입산브로일러에비하여어두운색즉, 명도가낮은정강이색을가진한국산재래닭으로의육종개량은보다효율적이고경제적으로추진할수 있으며, 이를통하여재래닭사육농가의소득증대및 경영개선에이바지할수 있다.
Abstract translation: 本发明涉及一种参与韩国本地鸡的柄色的DNA标记的开发，目的是开发用于预测和鉴定早期韩国本地鸡的柄颜色的遗传能力的DNA标记，由此被使用 用于韩国本地鸡的育种改良或经济性状改良方案。 在使用DNA标记作为韩国本地鸡的早期选择工具来检查韩国本地鸡的柄颜色的情况下，本发明能够更有效和经济地推动育种改进成为具有较深颜色的韩国本地鸡， 即与具有客户对韩国本地鸡所需要的柄颜色的进口肉鸡相比，具有低亮度柄的颜色，特别是接近亮黄色的黄色。 因此，本发明能够促进本地养鸡场的收入增加和管理改善。
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公开(公告)号：KR1020150010905A
公开(公告)日：2015-01-29
申请号：KR1020130085703
申请日：2013-07-19
Applicant: 경상대학교산학협력단 , 충남대학교산학협력단
CPC classification number: C12Q1/6827 , C12Q2600/124 , C12Q2600/156 , G01N33/5308
Abstract: 본 발명은 바이오마커에 관한 것으로 더 상세하게는 새로운 유두수 예측용 바이오마커 및 그의 선별방법을 제공한다.
Abstract translation: 本发明涉及生物标志物。 提供了一种用于预测猪的乳头数量的新型生物标志物及其选择方法。 发现猪的位置候选基因HDDC3，LOC100156276，LOC100155863，ANPEP，SCAMP2与乳头数有关系。 根据本发明的一个方面，提供了一种用于预测猪的乳头数的单核苷酸多态性生物标志物，其包括至少一种选自单核苷酸多态性rs80974417，rs80799165，rs80997276，rs80998243， 和rs325548834。
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公开(公告)号：KR1020140049247A
公开(公告)日：2014-04-25
申请号：KR1020120115246
申请日：2012-10-17
Applicant: 충남대학교산학협력단 , 경상대학교산학협력단
CPC classification number: C12Q1/6888 , C12Q2525/151 , C12Q2600/158
Abstract: The present invention relates to a method for individually identifying Korean native chickens using a microsatellite marker, and more specifically, provides a method for individually identifying Korean native chickens, the method comprising: a step of performing PCR amplification using microsatellite markers obtained by combining one or more sets of a first set consisting of microsatellite markers LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, and ADL0304, and a second set consisting of microsatellite markers LEI0251, ADL0259, ADL0021, MCW0104, MCW0261, MCW0087, ADL0292, GCT0016, MCW0029, MCW0145, LEI0107, ADL0304, ADL0324, MCW0063, and MCW0264; and determining the genotype by performing electrophoresis on the product amplified in the step of performing PCR amplification to thereby detect alleles and analyze sizes thereof. The method according to the present invention enables individual identification and paternity identification in a more accurate manner than the morphological classification, in terms of identification of Korean native chickens, by using the microsatellite markers having high individual identification. Therefore, the method of the present invention is applied to the individual identification of Korean native chickens to produce positive effects in the preservation of Korean native chickens and reliability thereof, thereby preventing illegal distribution and contributing to the development of native chicken industry and an increase in the farm income.
Abstract translation: 本发明涉及使用微卫星标记单独鉴定韩国本地鸡的方法，更具体地说，提供了一种用于单独鉴定韩国天然鸡的方法，所述方法包括：使用通过组合一个或多个的微卫星标记进行PCR扩增的步骤， 由微卫星标记LEI0251，ADL0259，ADL0021，MCW0104，MCW0261，MCW0087，ADL0292，GCT0016，MCW0029，MCW0145，LEI0107和ADL0304组成的第一组的更多组，以及由微卫星标记LEI0251，ADL0259，ADL0021，MCW0104组成的第二组 ，MCW0261，MCW0087，ADL0292，GCT0016，MCW0029，MCW0145，LEI0107，ADL0304，ADL0324，MCW0063和MCW0264; 以及通过对进行PCR扩增的步骤中扩增的产物进行电泳从而检测等位基因并分析其大小来确定基因型。 根据本发明的方法，通过使用具有高个体鉴定的微卫星标记，在韩国本地鸡的鉴定方面，以比形态分类更准确的方式进行个体鉴定和亲戚鉴定。 因此，本发明的方法适用于韩国本土鸡的个体鉴定，从而在保护韩国本国鸡中产生积极作用及其可靠性，从而防止非法分配和促进本地鸡业发展，并增加 农场收入。
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Patent Agency Ranking16.发明授权
公开(公告)号：KR101273333B1
公开(公告)日：2013-06-11
申请号：KR1020120075835
申请日：2012-07-12
Applicant: 충남대학교산학협력단
CPC classification number: C12Q1/683 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A method for analyzing SNP of SCD(Stearoyl-CoA Desaturase) gene of a chicken is provided to produce and select high quality chickens, to improve farm income, and to increase sales. CONSTITUTION: A method for selecting a chicken with higher unsaturated fatty acid content than a chicken with heterozygous genotype at 2nd exon of chicken SCD gene comprises: a step of collecting a DNA sample from the chicken; a step of amplifying the DNA sample using the primer set of sequence numbers 1 and 2; a step of treating the amplified product with Acil; and a step of performing electrophoresis and identifying whether SNP of the 2nd exon of SCD is a homozygous genotype.
Abstract translation: 目的：提供一种分析鸡的SCD（硬脂酰CoA去饱和酶）基因SNP的方法，以生产和选择优质鸡，提高农民收入，增加销售。 构成：选择鸡SCD基因第二外显子上具有杂合子基因型的鸡的不饱和脂肪酸含量高的鸡的方法包括：从鸡收集DNA样品的步骤; 使用序列号1和2的引物组扩增DNA样品的步骤; 用Acil处理扩增产物的步骤; 以及进行电泳并鉴定SCD的第二外显子的SNP是否是纯合基因型的步骤。
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公开(公告)号：KR1020130118650A
公开(公告)日：2013-10-30
申请号：KR1020120041710
申请日：2012-04-20
Applicant: 경상대학교산학협력단 , 충남대학교산학협력단
CPC classification number: C12Q1/6888 , C12Q2600/124 , C12Q2600/156
Abstract: PURPOSE: A SNP marker for predicting fatty acid composition of a crossbreed pig is provided to predict fatty acid composition, especially for a composition of unsaturated fatty acid and saturated fatty acid, thereby utilizing the SNP marker for selection of healthy pig entity and high value pork production. CONSTITUTION: A polynucleotide or corresponding polynucleotide for predicting fatty acid composition of a crossbreed pig is selected from polynucleotide having nucleic acid sequence of sequence number 1. The polynucleotide or corresponding polynucleotide comprises 0 or more of successive nucleic acids which include 817th of the sequence number 1 or 14384th SNP related base. The crossbreed pig is a crossbreed between Korea native kind and Landrace. The crossbreed pig is F_2. A polynucleotide which specifically mix with the polynucleotide or the corresponding polynucleotide is provided.
Abstract translation: 目的：提供用于预测杂交猪的脂肪酸组成的SNP标记，以预测脂肪酸组成，特别是对于不饱和脂肪酸和饱和脂肪酸的组成，从而利用SNP标记物选择健康猪实体和高价值猪肉 生产。 构成：用于预测杂交猪的脂肪酸组成的多核苷酸或相应的多核苷酸选自具有序列号1的核酸序列的多核苷酸。多核苷酸或相应的多核苷酸包含0或更多个连续核酸，其包含序列号1的第817位 或第14384位SNP相关基地。 杂交猪是韩国本土和兰德拉斯之间的杂交种。 杂交猪是F_2。 提供了与多核苷酸或相应多核苷酸特异性混合的多核苷酸。
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公开(公告)号：KR101312480B1
公开(公告)日：2013-10-01
申请号：KR1020120158428
申请日：2012-12-31
Applicant: 대한민국(농촌진흥청장) , 경상대학교산학협력단 , 충남대학교산학협력단
CPC classification number: C12Q1/6827 , C12Q1/6837 , C12Q1/6844
Abstract: PURPOSE: A single nucleotide polymorphism (SNP) marker is provided to objectively evaluate the number of ribs in a pig, which is hard to distinguish with naked eyes, thereby establishing distribution of pork. CONSTITUTION: An SNP marker for determining the number of ribs in a pig contains a polynucleotide with 5-100 continuous bases including 374th base which is A or C in exon 32 of LTBP2 gene or a complementary polynucleotide thereof. A composition for determining the number of ribs in a pig contains an agent for determining or amplifying the SNP marker. A kit for determining the number of ribs in a pig contains the composition. [Reference numerals] (AA) C/C genotype; (BB) A/C genotype; (CC) A/A genotype
Abstract translation: 目的：提供单核苷酸多态性（SNP）标记，以客观评价猪肉中的肋骨数量，这很难用肉眼辨别，从而建立猪肉分布。 构成：用于确定猪排数的SNP标记含有具有5-100个连续碱基的多核苷酸，包括第374位碱基，其是LTBP2基因的外显子32中的A或C或其互补多核苷酸。 用于确定猪的肋数的组合物含有用于确定或扩增SNP标记的试剂。 用于确定猪的肋数的试剂盒包含组合物。 （AA）C / C基因型; （BB）A / C基因型; （CC）A / A基因型
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公开(公告)号：KR100980809B1
公开(公告)日：2010-09-10
申请号：KR1020080022720
申请日：2008-03-12
Applicant: 충남대학교산학협력단
Abstract: 본 발명은 돼지 유전자에 삽입되어 있는 PERV의 연접서열 및 이를 이용한 PERV 검출용 프라이머 쌍에 관한 것으로서, 연접서열인 서열번호 1 내지 14 중 어느 하나를 포함하는 염기서열과, ① 상기 연접서열을 포함하는 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드와, ② PERV 유전자 염기서열에 상보적인 서열을 갖는 올리고 뉴클레오티드로 이루어진 돼지 게놈 내 융합된 PERV 검출을 위한 프라이머 쌍에 관한 것이다.
본 발명에 의하면 돼지의 조직 또는 장기로부터 이종이식 시 문제의 소지가 있는 PERV의 존재를 사전에 검출할 수 있으며, 더 나아가 해당 유전자를 녹아웃(knock-out)시키는 데 이용할 수 있다.
돼지 내인성 레트로바이러스(PERV), 장기이식, 검출, 프라이머, 유전좌위, knock-out-
公开(公告)号：KR1020090097526A
公开(公告)日：2009-09-16
申请号：KR1020080022720
申请日：2008-03-12
Applicant: 충남대학교산학협력단
CPC classification number: C12Q1/686
Abstract: A sequence of PERV (porcine endogenous retrovirus) and PERV primer pair using the same are provided to previously detect the presence of PERV and use for knock-out of a gene. A 3'-, or 5'-sequence to a porcine endogenous retrovirus (PERV) which is a sequence selected from the sequence numbers 1 to 14. A primer pair for detecting PERV comprises an oligonucleotide which has complementary sequence to the sequence numbers 1 to 14 and an oligonucleotide which has complementary sequence to the PERV gene sequence. The primer pair is used for detecting PERV through PCR (polymerase chain reaction) from porcine tissue or organ.
Abstract translation: 提供了使用其的PERV（猪内源性逆转录病毒）和PERV引物对的序列，以便先前检测PERV的存在并用于敲除基因。 与选自序列号1〜14的序列的猪内源性逆转录病毒（PERV）的3'-或5'序列。检测PERV的引物对包含与序列号1〜 14和与PERV基因序列具有互补序列的寡核苷酸。 引物对用于通过PCR（聚合酶链反应）从猪组织或器官检测PERV。
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