公开(公告)号：AT538138T

公开(公告)日：2012-01-15

申请号：AT05760334

申请日：2005-03-16

Applicant: AMNIS CORP

Inventor： GEORGE THADDEUS ,  BASIJI DAVID ,  FROST KEITH ,  HALL BRIAN ,  ORTYN WILLIAM ,  SEO MICHAEL

IPC: C07K16/28 ,  G01N21/64 ,  G01N33/50 ,  G01N33/569 ,  G01N33/58

公开(公告)号：AU1191302A

公开(公告)日：2002-04-22

申请号：AU1191302

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM ,  BASIJI DAVID

IPC: C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/14 ,  G01N21/03 ,  G01N21/05 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14 ,  G01N1/10 ,  G01N21/00 ,  G01B11/26

Abstract: A multipass cavity system employs a beam of light that is reflected back and forth between reflective surfaces a plurality of times, illuminating a different portion of the field of view with each pass until the light exits the reflection cavity. The "recycling" of the light beam substantially improves the SNR of the detection system. The present invention is a beam alignment system for use in such a multipass cavity, correcting misalignment in four degrees of freedom; the horizontal and vertical axes, and angle in each of the vertical and horizontal axes. Angular or positional errors lateral to the direction of the beam can dramatically affect performance. The present invention enables the measurement and adjustment of each degree of freedom independently, in order to make beam steering corrections and maintain optical alignment. This is accomplished in an automated, closed-loop feedback control system.

公开(公告)号：EP2089413A4

公开(公告)日：2009-10-28

申请号：EP02759066

申请日：2002-02-21

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM ,  BASIJI DAVID

IPC: G01N21/64 ,  C07H21/04 ,  C12M1/34 ,  C12Q1/68 ,  G01J3/44 ,  G01N15/14 ,  G01N27/447

CPC classification number: G01N27/44726 ,  C12Q1/6841 ,  G01J3/2803 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6456 ,  G01N2015/1472 ,  G01N2021/6421 ,  G01N2021/6441 ,  C12Q2565/102 ,  C12Q2537/143

公开(公告)号：EP1257794A4

公开(公告)日：2006-12-13

申请号：EP00973466

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： BASIJI DAVID ,  ORTYN WILLIAM

IPC: C12Q1/02 ,  G01J3/28 ,  G01N15/02 ,  G01N15/06 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G01N33/48 ,  G02B7/28 ,  G02B27/00 ,  G02B27/14 ,  G06K9/00 ,  G06T1/00 ,  G01J3/18 ,  G01J3/30

CPC classification number: G06K9/00127 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/147 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/85 ,  G01N2015/0294 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G02B7/28 ,  G02B27/145 ,  G02B27/148

Abstract: Light (30) from an object (24) such as a cell moving through an imaging system (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light (30) can be emitted from a luminous object (24) or can be light from a light source that has been scattered by the object or can be a fluorescent emission by one or more FISH probes, frequently used to detect substances within cells. Further, light that is absorbed or reflected by the object can also be used to produce images for determining specific characteristics of the object. The movement of the object matches the rate at which a signal is read from the TDI detector. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and spectrally dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：CA2445960A1

公开(公告)日：2002-12-19

申请号：CA2445960

申请日：2002-02-21

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM ,  BASIJI DAVID

IPC: C12Q1/68 ,  C12Q1/6841 ,  G01N27/447 ,  G01N21/84 ,  C12Q1/02 ,  C07H21/04 ,  C12Q1/04 ,  C12M1/34 ,  G01N33/532 ,  G01N33/533 ,  G01N21/55 ,  G01N21/64 ,  G01N21/89

Abstract: A labeling method that labels an object or specific features of an object wi th labeled probes that provide a multiplexed signal that can be analyzed by spectral decomposition. This binary and higher encoding scheme can be employ ed to label components of biological cells. In each encoding scheme, labeled probes that selectively bind to a specific feature are required. The labeled probes include a binding element that binds to the feature, and at least one signaling component that generates a detectable signal, preferably a spectra l signature. In one embodiment, adding multiple fluorescent dye molecules to each binding element provides the multiplexed signal. In another embodiment, adding only one signal compound to each binding element provides the multiplexed signal, such that some of the binding elements have a different signal compound added. The different signal compounds provide the multiplexe d signal.

公开(公告)号：AU1189802A

公开(公告)日：2002-04-22

申请号：AU1189802

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  C07B61/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

公开(公告)号：AU1196901A

公开(公告)日：2001-07-31

申请号：AU1196901

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： BASIJI DAVID ,  ORTYN WILLIAM

IPC: C12Q1/02 ,  G01J3/28 ,  G01N15/02 ,  G01N15/06 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G01N33/48 ,  G02B7/28 ,  G02B27/00 ,  G02B27/14 ,  G06K9/00 ,  G06T1/00 ,  G01J3/18 ,  G01J3/30

Abstract: Light from an object such as a cell moving through an imaging system is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector. The light can be emitted from a luminous object or can be light from a light source that has been scattered by the object or can be a fluorescent emission by one or more FISH probes, frequently used to detect substances within cells. Further, light that is absorbed or reflected by the object can also be used to produce images for determining specific characteristics of the object. The movement of the object matches the rate at which a signal is read from the TDI detector. Multiple objects passing through the imaging system can be imaged, producing both scatter images and spectrally dispersed images at different locations on one or more TDI detectors.

公开(公告)号：EP1330650A4

公开(公告)日：2008-03-19

申请号：EP01979992

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC: G01N21/64 ,  G01N33/543 ,  B01J19/00 ,  C07B61/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

CPC classification number: G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2600/156 ,  C40B20/04 ,  C40B60/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2015/0294 ,  G01N2015/1018 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G01N2021/6421 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2565/626 ,  C12Q2563/149 ,  C12Q2563/107

Abstract: Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).

公开(公告)号：AU2001211994B2

公开(公告)日：2004-11-18

申请号：AU2001211994

申请日：2000-10-12

Applicant: AMNIS CORP

Inventor： BASIJI DAVID ,  ORTYN WILLIAM

IPC: G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G02B7/28 ,  G02B27/14 ,  G01J003/12 ,  G01J003/51 ,  G01J003/28

Abstract: Light from an object (24), such as a cell, moving through an imaging system (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light can be emitted from a luminous object or can be light from a light source that has been scattered or not absorbed by the object or can include a light emission by one or more probes within or on the object. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：AU2001297843A1

公开(公告)日：2002-12-23

申请号：AU2001297843

申请日：2001-10-12

Applicant: AMNIS CORP

Inventor： ORTYN WILLIAM ,  BASIJI DAVID

IPC: C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  C12Q1/6816 ,  G01N15/02 ,  G01N15/14 ,  G01N21/03 ,  G01N21/05 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14 ,  G01J3/12 ,  G01J3/51

Abstract: A system and method for high numeric aperture imaging systems includes a splitter, a defocusing system, and a combiner. The splitter reflects a portion of collected light and transmits another portion of the collected light. The defocusing system is configured to modify optical power of either the transmitted portion or reflected portion of the collected light. The combiner is oriented with respect to a mechanical angle. The combiner recombines portions of the transmitted portion and the reflected portion such that the transmitted portion and reflected portion are subsequently transmitted being separated by an optical separation angle based upon the mechanical angle of orientation of the combiner. Various other implementations are used to maintain focus with regards to the imaging systems involved.