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    Multiple promoters and the use thereof for gene expression
    11.
    发明专利
    Multiple promoters and the use thereof for gene expression 未知

    公开(公告)号：AU2005321577A1

    公开(公告)日：2006-07-06

    申请号：AU2005321577

    申请日：2005-12-21

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  SCHRODER HARTWIG ,  HEROLD ANDREA ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA

    IPC: C12N15/77 ,  C07K14/34

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS
    13.
    发明专利
    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS 未知

    公开(公告)号：CA2615315C

    公开(公告)日：2015-10-06

    申请号：CA2615315

    申请日：2006-07-18

    Applicant: BASF AG

    Inventor： ZELDER OSKAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

    IPC: C12P13/12

    Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a .DELTA.metF organism or a .DELTA.metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/Met.EPSILON. and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A .DELTA.metF .DELTA.metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    16.
    发明专利
    未知

    公开(公告)号：BRPI0612900A2

    公开(公告)日：2014-10-14

    申请号：BRPI0612900

    申请日：2006-07-18

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  YOCUM R ROGERS ,  ZELDER OSKAR ,  SCHROEDER HARTWIG ,  WILLIAMS MARK K

    IPC: C12P13/12

    Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &Dgr;metF organism or a &Dgr;metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &Dgr;metF &Dgr;metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    17.
    发明专利
    未知

    公开(公告)号：BRPI0520842A2

    公开(公告)日：2012-07-03

    申请号：BRPI0520842

    申请日：2005-12-21

    Applicant: BASF AG

    Inventor： HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA

    IPC: C12N15/77 ,  C07K14/34

    Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

    20.
    发明专利
    未知

    公开(公告)号：BRPI0613660A2

    公开(公告)日：2012-11-06

    申请号：BRPI0613660

    申请日：2006-07-18

    Applicant: BASF AG

    Inventor： HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  YOCUM R ROGERS ,  ZELDER OSKAR ,  SCHROEDER HARTWIG ,  WILLIAMS MARK K

    IPC: C12P13/12 ,  C07K14/32 ,  C07K14/34 ,  C12N5/10

    Abstract: The present invention pertains to improved microorganisms and methods for the production of methionine and other sulfur containing fine chemicals using the metI gene from Bacillus subtilis or a gene related to metI. In some embodiments of the present invention, the metI gene or another gene is integrated in a fashion that allows for co-production of a water soluble compound such as methionine or other amino acid and a caortenoid compound.

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