公开(公告)号：CA2506999C

公开(公告)日：2014-01-07

申请号：CA2506999

申请日：2003-11-27

Applicant: BASF AG

Inventor： KESSELER MARIA ,  ZELINSKI THOMAS ,  HAUER BERNHARD

IPC: C12N15/63 ,  C12N15/70

Abstract: The invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells. According to said methods, at least one DNA construct that can be episomally replicated in said host cells and comprises a nucleic acid sequence that is to be expressed is introduced into the host cells under the transcriptional control of an L-rhamnose-inducible promoter, said promoter being heterologous relative to said nucleic acid sequence, and expression of the nucleic acid sequence is induced by adding R-rhamnose. The inventive methods are characterized by the fact that the prokaryotic host cell used is at least deficient in an L-rhamnose isomerase.

公开(公告)号：AU2003288189B8

公开(公告)日：2009-06-25

申请号：AU2003288189

申请日：2003-11-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  ZELINSKI THOMAS ,  KESSELER MARIA

IPC: C12N15/70 ,  C12N15/63

公开(公告)号：CA2590660A1

公开(公告)日：2006-07-13

申请号：CA2590660

申请日：2005-12-31

Applicant: BASF AG

Inventor： STUERMER RAINER ,  BREUER MICHAEL ,  DAEUWEL JUERGEN ,  KESSELER MARIA ,  ACHATZ BRIGITTE

IPC: C07D333/20 ,  B01D3/12

Abstract: The present invention relates to a method for purifying (1S)-3-METHYLAMINO-1-(2-THIENYL)-1-PROPANOL by distilling a reaction mixture which comprises between 25 and 99% by weight of (IS)-3-METHYLAMINO-1-(2-THIENYL)-1-PROPANOL.

公开(公告)号：CA2521288A1

公开(公告)日：2004-10-21

申请号：CA2521288

申请日：2004-04-06

Applicant: BASF AG

Inventor： KESSELER MARIA ,  ALTHOFER HENNING

IPC: C12N9/04 ,  C12N15/53 ,  C12P7/04 ,  C12P13/00 ,  C12P17/00 ,  C12P41/00 ,  C07D333/20

Abstract: The invention relates to proteins that have an enzymatic activity for reduci ng substituted alkanones, such as 3-methylamino-1-(2-thienyl)-propane-1-on. The invention also relates to nucleic acids that encode these proteins, to nucle ic acid constructs, vectors, genetically modified microorganisms and to methods for producing substituted (S) alkanols, such as for example (S)-3-methylamin o- 1-(2-thienyl)-(S)-propanol.

公开(公告)号：CA2506999A1

公开(公告)日：2004-06-17

申请号：CA2506999

申请日：2003-11-27

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  KESSELER MARIA ,  ZELINSKI THOMAS

IPC: C12N15/63 ,  C12N15/70

Abstract: The invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells. According to said methods, at least one DNA construc t that can be episomally replicated in said host cells and comprises a nucleic acid sequence that is to be expressed is introduced into the host cells unde r the transcriptional control of an L-rhamnose-inducible promoter, said promot er being heterologous relative to said nucleic acid sequence, and expression of the nucleic acid sequence is induced by adding R-rhamnose. The inventive methods are characterized by the fact that the prokaryotic host cell used is at least deficient in an L-rhamnose isomerase.

公开(公告)号：BR0015114A

公开(公告)日：2002-07-16

申请号：BR0015114

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：SI1570060T1

公开(公告)日：2007-12-31

申请号：SI200330976

申请日：2003-11-27

Applicant: BASF AG

Inventor： KESSELER MARIA ,  ZELINSKI THOMAS ,  HAUER BERNHARD

IPC: C12N15/00

公开(公告)号：AU782517B2

公开(公告)日：2005-08-04

申请号：AU1141601

申请日：2000-10-20

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：NO20021931L

公开(公告)日：2002-04-24

申请号：NO20021931

申请日：2002-04-24

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

Abstract: The present invention relates to proteins which have an enzymatic activity for hydrolyzing L-pantolactone. The invention further relates to nucleic acids which code for these proteins, to nucleic acid constructs, vectors, genetically modified microorganisms and to a process for preparing D-pantolactone.

公开(公告)号：NO20021931A

公开(公告)日：2002-04-24

申请号：NO20021931

申请日：2002-04-24

Applicant: BASF AG

Inventor： KESSELER MARIA ,  HAUER BERNHARD ,  FRIEDRICH THOMAS ,  MATTES RALF

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/16 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00 ,  C12R1/19

CPC classification number: C12P17/04 ,  C12N9/18 ,  C12P41/005