公开(公告)号：AU764060B2

公开(公告)日：2003-08-07

申请号：AU8364801

申请日：2001-10-26

Applicant: CEPHEID

Inventor： POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: C12M1/42 ,  G01N1/28 ,  G01N1/40

公开(公告)号：AT228242T

公开(公告)日：2002-12-15

申请号：AT99925705

申请日：1999-05-19

Applicant: CEPHEID

Inventor： CHRISTEL LEE A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A ,  YOUNG STEVEN J ,  CHANG RONALD ,  DORITY DOUGLAS B ,  HEBERT RAYMOND ,  KINTZ GREGORY J

IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

Abstract: An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel (2) having a chamber (10) for holding the mixture. The apparatus also includes a heat-exchanging module (37) having a pair of opposing thermal plates (34A, 34B) for receiving the vessel (2) between them and for heating and/or cooling the mixture contained in the vessel. The module (37) also includes optical excitation and detection assemblies (46, 48) positioned to optically interrogate the mixture. The excitation assembly (46) includes multiple light sources (100) and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly (48) includes multiple detectors (102) and a second set of filters for detecting light emitted from the chamber (10) in multiple emission wavelength ranges. The optics assemblies (46, 48) thus provide a multi-channel system for detcting a plurality of different target analytes in the mixture.

公开(公告)号：AU3075301A

公开(公告)日：2001-07-03

申请号：AU3075301

申请日：2000-12-15

Applicant: CEPHEID

Inventor： CHANG RONALD ,  DORITY DOUGLAS B ,  CHRISTEL LEE A ,  PETERSEN KURT E

IPC: B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C12Q1/68 ,  F28F13/00 ,  G01N21/03 ,  G01N35/00 ,  G01N35/04

Abstract: An apparatus for controlling the temperature of a reaction mixture contained in a reaction vessel, wherein the vessel includes a reaction chamber and at least one port for adding fluid to the chamber, and wherein the chamber has at least one flexible wall, comprises a) a thermal surface for contacting the flexible wall; b) a platen for heat sealing the vessel to seal the port closed and increase the pressure in the chamber, wherein the pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface; and c) at least one thermal element for heating or cooling the thermal surface to induce a temperature change within the chamber.

公开(公告)号：DE60039640D1

公开(公告)日：2008-09-04

申请号：DE60039640

申请日：2000-12-15

Applicant: CEPHEID

Inventor： CHANG RONALD ,  DORITY DOUGLAS B ,  CHRISTEL LEE A ,  PETERSEN KURT E

IPC: B01L7/00 ,  B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/68 ,  F28F13/00 ,  G01N21/03 ,  G01N35/00 ,  G01N35/04

Abstract: An apparatus for controlling the temperature of a reaction mixture contained in a reaction vessel, wherein the vessel includes a reaction chamber and at least one port for adding fluid to the chamber, and wherein the chamber has at least one flexible wall, comprises a) a thermal surface for contacting the flexible wall; b) a platen for heat sealing the vessel to seal the port closed and increase the pressure in the chamber, wherein the pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface; and c) at least one thermal element for heating or cooling the thermal surface to induce a temperature change within the chamber.

公开(公告)号：DE69839709D1

公开(公告)日：2008-08-21

申请号：DE69839709

申请日：1998-12-24

Applicant: CEPHEID

Inventor： POURAHMADI FARZAD ,  MCMILLIAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: B01J19/00 ,  G01N31/20 ,  B01D11/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.

公开(公告)号：AT400358T

公开(公告)日：2008-07-15

申请号：AT01118242

申请日：1998-12-24

Applicant: CEPHEID

Inventor： POURAHMADI FARZAD ,  MCMILLIAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: B01J19/00 ,  G01N31/20 ,  B01D11/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.

公开(公告)号：CA2282623C

公开(公告)日：2007-05-22

申请号：CA2282623

申请日：1998-03-02

Applicant: CEPHEID

Inventor： YUAN ROBERT ,  POURAHMADI FARZAD ,  PETERSEN KURT E ,  CHANG RONALD ,  NORTHRUP M ALLEN ,  YOUNG STEVEN ,  CHRISTEL LEE A ,  DORITY DOUGLAS B ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A

IPC: C12M1/00 ,  G01N1/31 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/68 ,  F28F3/12 ,  F28F21/04 ,  G01N21/03 ,  G01N21/15 ,  G01N21/64 ,  G01N35/00

Abstract: The present invention provides a reaction vessel and apparatus for performin g heat-exchanging reactions. The vessel has a chamber for holding a sample, th e chamber being defined by two opposing major walls and a plurality of minor walls joining t he major walls to each other, at least two of the walls being light transmissive to provide optical windows to the chamber. The apparatus comprises at least one heating surface for contacting at least one of the major walls, a heat source for heating the surface, and optics positione d to optically interrogate the chamber while the heating surface is in contact with at leas t one of the major walls. The optics include at least one light source for transmitting light t o the chamber through a first one of the light transmissive walls and at least one detecto r for detecting light exiting the chamber through a second one of the light transmissive walls.

公开(公告)号：AT279260T

公开(公告)日：2004-10-15

申请号：AT00990945

申请日：2000-12-15

Applicant: CEPHEID

Inventor： CHANG RONALD ,  DORITY DOUGLAS B ,  CHRISTEL LEE A ,  PETERSEN KURT E

IPC: B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  C12Q1/68 ,  F28F13/00 ,  G01N21/03 ,  G01N35/00 ,  G01N35/04

Abstract: An apparatus for controlling the temperature of a reaction mixture contained in a reaction vessel, wherein the vessel includes a reaction chamber and at least one port for adding fluid to the chamber, and wherein the chamber has at least one flexible wall, comprises a) a thermal surface for contacting the flexible wall; b) a platen for heat sealing the vessel to seal the port closed and increase the pressure in the chamber, wherein the pressure increase in the chamber is sufficient to force the flexible wall to conform to the thermal surface; and c) at least one thermal element for heating or cooling the thermal surface to induce a temperature change within the chamber.

公开(公告)号：AU758407B2

公开(公告)日：2003-03-20

申请号：AU1947299

申请日：1998-12-24

Applicant: CEPHEID

Inventor： POURAHMADI FARZAD ,  MCMILLAN WILLIAM A ,  CHING JESUS ,  CHANG RONALD ,  CHRISTEL LEE A ,  KOVACS GREGORY T A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E

IPC: G01N31/20 ,  B01D11/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  C12M1/26 ,  C12M1/33 ,  C12M1/34 ,  C40B60/14 ,  G01N1/28 ,  G01N30/60 ,  G01N33/50 ,  G01N35/08 ,  G01N37/00

Abstract: A cartridge (101) for separating a desired analyte from a fluid sample includes a sample port (103) and a sample flow path extending from the port through the body of the cartridge. The sample flow path includes at least one flow-through component (122), e.g. filter paper or a microfabricated chip, for capturing the desired analyte from the sample as the sample flows through the cartridge. The cartridge also includes an elution flow path for carrying elution fluid through the component (122) to release captured analyte from the component into the elution fluid. The elution flow path diverges from the sample flow path after passing through the component (122). Flow controllers (41A and 41B) direct the remaining fluid sample into a waste chamber (139) after the sample flows through the component (122) and direct the elution fluid and eluted analyte into a reagent chamber (141) and reaction chamber (143). The continuous-flow design of the cartridge permits the rapid processing of a fluid sample that is larger in volume than any interactive region within the cartridge, allowing increased sensitivity in the detection of low copy concentrations of analytes, such as nucleic acid. A preferred embodiment relates to a device for lysing components of a fluid sample, the device comprising inter alia, an ultrasonic transducer.

公开(公告)号：AU746069B2

公开(公告)日：2002-04-11

申请号：AU4194099

申请日：1999-05-19

Applicant: CEPHEID

Inventor： CHRISTEL LEE A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A ,  YOUNG STEVEN J ,  CHANG RONALD ,  DORITY DOUGLAS B ,  HEBERT RAYMOND ,  KINTZ GREGORY J

IPC: G01N21/64 ,  G01N21/01 ,  G01N21/03

Abstract: An apparatus for thermally controlling and optically interrogating a reaction mixture includes a vessel (2) having a chamber (10) for holding the mixture. The apparatus also includes a heat-exchanging module (37) having a pair of opposing thermal plates (34A, 34B) for receiving the vessel (2) between them and for heating and/or cooling the mixture contained in the vessel. The module (37) also includes optical excitation and detection assemblies (46, 48) positioned to optically interrogate the mixture. The excitation assembly (46) includes multiple light sources (100) and a set of filters for sequentially illuminating labeled analytes in the mixture with excitation beams in multiple excitation wavelength ranges. The detection assembly (48) includes multiple detectors (102) and a second set of filters for detecting light emitted from the chamber (10) in multiple emission wavelength ranges. The optics assemblies (46, 48) thus provide a multi-channel system for detcting a plurality of different target analytes in the mixture.