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11.发明公开COPY NUMBER VARIATION DETERMINATION, METHODS AND SYSTEMS 有权VERFAHREN UND系统ZUR BESTIMMUNG VON GENKOPIEZAHLVARIANTEN
公开(公告)号：EP2198293A4
公开(公告)日：2011-02-09
申请号：EP08829141
申请日：2008-09-08
Applicant: FLUIDIGM CORP
Inventor： RAMAKRISHNAN RAMESH
IPC: G01N33/48
CPC classification number: C12Q1/6869 , C12Q1/6851 , C12Q2545/101 , C12Q2527/143
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12.发明公开PROXIMITY ASSAYS FOR DETECTING NUCLEIC ACIDS AND PROTEINS IN A SINGLE CELL 审中-公开NEAR测定为了蛋白的单个细胞和核酸检测的
公开(公告)号：EP3041957A4
公开(公告)日：2017-03-29
申请号：EP14842635
申请日：2014-09-04
Applicant: FLUIDIGM CORP
Inventor： EGIDIO CAMILA , RAMAKRISHNAN RAMESH , RUFF DAVID
IPC: C12Q1/68
CPC classification number: C12Q1/6804 , C12Q1/6848 , C12Q1/6851 , C12Q2563/179 , C12Q2565/543
Abstract: Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays.
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公开(公告)号：EP2569447A4
公开(公告)日：2013-11-27
申请号：EP11780930
申请日：2011-05-16
Applicant: FLUIDIGM CORP , PIEPRZYK MARTIN
Inventor： PIEPRZYK MARTIN , JONES ROBERT C , LIVAK KENNETH J , MAY ANDREW , MIR ALAIN , QIN JIAN , RAMAKRISHNAN RAMESH , SPURGEON SANDRA , WANG JUN , ZIMMERMANN BERNHARD G
CPC classification number: C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , G01N33/542 , G01N33/582 , G01N33/6893 , G01N2800/368 , C12Q2521/301 , C12Q2521/501 , C12Q2525/155 , C12Q2535/131 , C12Q2537/143 , C12Q2537/16 , C12Q2563/173
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公开(公告)号：SG11202001088PA
公开(公告)日：2020-03-30
申请号：SG11202001088P
申请日：2018-08-20
Applicant: FLUIDIGM CORP
Inventor： QIN JIAN , CHEN PEILIN , RAMAKRISHNAN RAMESH
IPC: C12N15/10 , C12Q1/6806
Abstract: This invention provides a technology for isolating nucleic acids from wax-embedded samples that is superior to the current state of the art. Standard protocols with this objective typically comprise dissolving the wax-embedded sample in an organic solvent, extracting nucleic acids from the organic solvent into an aqueous buffer, and isolating the nucleic acids from the aqueous buffer. The technology described here includes using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.
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公开(公告)号：SG10201908167YA
公开(公告)日：2019-10-30
申请号：SG10201908167Y
申请日：2014-09-04
Applicant: FLUIDIGM CORP
Inventor： EGIDIO CAMILA , RAMAKRISHNAN RAMESH , RUFF DAVID
Abstract: PROXIMITY ASSAYS FOR DETECTING NUCLEIC ACIDS AND PROTEINS IN A SINGLE CELL Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays. [Figure 1]
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Patent Agency Ranking
公开(公告)号：CA3072463A1
公开(公告)日：2019-02-21
申请号：CA3072463
申请日：2018-08-20
Applicant: FLUIDIGM CORP
Inventor： QIN JIAN , CHEN PEILIN , RAMAKRISHNAN RAMESH
IPC: C12N15/10 , C12Q1/6806
Abstract: Disclosed herein is a method and kit thereof for isolating nucleic acids from wax-embedded samples using hexadecane as the solvent to dissolve the sample, precipitating and washing the extracted nucleic acids using ethanol, and dissolving the nucleic acids in a lysis buffer that includes NP40 and SDS. By implementing the reagents and techniques described in this disclosure, the user can obtain a product that has better yield, less degradation, and contains more unique mRNA transcripts for subsequent sequencing and analysis.
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公开(公告)号：CA2922926A1
公开(公告)日：2015-03-12
申请号：CA2922926
申请日：2014-09-04
Applicant: FLUIDIGM CORP
Inventor： EGIDIO CAMILA , RAMAKRISHNAN RAMESH , RUFF DAVID
IPC: C12Q1/68
Abstract: Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays.
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公开(公告)号：BRPI0816393A2
公开(公告)日：2015-03-03
申请号：BRPI0816393
申请日：2008-09-08
Applicant: FLUIDIGM CORP
Inventor： RAMAKRISHNAN RAMESH
IPC: G01N33/48
Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.
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公开(公告)号：CA2698545A1
公开(公告)日：2009-03-12
申请号：CA2698545
申请日：2008-09-08
Applicant: FLUIDIGM CORP
Inventor： RAMAKRISHNAN RAMESH
IPC: G01N33/48
Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.
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公开(公告)号：AU2008295992A1
公开(公告)日：2009-03-12
申请号：AU2008295992
申请日：2008-09-08
Applicant: FLUIDIGM CORP
Inventor： RAMAKRISHNAN RAMESH
IPC: G01N33/48
Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.
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