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    MULTIFUNCTIONAL PROBE-PRIMERS
    5.
    发明申请
    MULTIFUNCTIONAL PROBE-PRIMERS 审中-公开
    多功能探测器

    公开(公告)号：WO2012106668A3

    公开(公告)日：2013-01-10

    申请号：PCT/US2012023870

    申请日：2012-02-03

    Applicant: FLUIDIGM CORP ,  LIVAK KENNETH J

    Inventor： LIVAK KENNETH J

    IPC: C12Q1/68 ,  C07H21/00

    CPC classification number: C12Q1/6876 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2565/1015

    Abstract: Methods and reagents for detection and analysis of nucleic acids are provided. Certain methods involves an encoding amplification in which a target sequence is associated with probe-binding sequences and optionally with indexing sequences, (2) an optional distribution step in which the product of the encoding amplification is split into multiple aliquots, and (3) a decoding and detection step in which the presence, absence, quantity, or relative amount of the target sequence in the aliquots is determined. The detection step makes use of a multifunctional "self-digesting" molecular probe comprising a primer polynucleotide and a probe oligonucleotide, linked in a 5'-5' orientation.

    Abstract translation: 提供了用于检测和分析核酸的方法和试剂。 某些方法涉及编码扩增，其中靶序列与探针结合序列和任选的索引序列相关，（2）任选的分布步骤，其中编码扩增的产物被分成多个等分试样，和（3）a 解码和检测步骤，其中确定等分试样中靶序列的存在，不存在，数量或相对量。 检测步骤使用包含以5'-5'取向连接的引物多核苷酸和探针寡核苷酸的多功能“自消化”分子探针。

    SINGLE-CELL TRANSCRIPT SEQUENCING
    8.
    发明专利
    SINGLE-CELL TRANSCRIPT SEQUENCING 未知

    公开(公告)号：CA3027423A1

    公开(公告)日：2018-01-18

    申请号：CA3027423

    申请日：2017-07-12

    Applicant: FLUIDIGM CORP

    Inventor： LIVAK KENNETH J ,  FEKETE RICHARD A

    IPC: C12Q1/68

    Abstract: Described herein are methods for preparing DNA templates for single-cell transcript sequencing of RNA from a population of cells. The methods entail distributing cells from the population into separate reaction volumes so that a plurality of separate reaction volumes each contain a single, isolated cell, wherein the cells have been treated with a fixative prior to distribution. The isolated cells are then permeabilized or disrupted, and cDNA is prepared by reverse transcript, followed by amplification. Also provided is a novel chemistry for efficient production of DNA templates from T-cell receptors or immunoglobulins in single cells.

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