METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS
    5.
    发明申请
    METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS 审中-公开
    生物样品分析方法和设备

    公开(公告)号:WO2008067552A3

    公开(公告)日:2008-11-27

    申请号:PCT/US2007086146

    申请日:2007-11-30

    CPC classification number: C12Q1/686 C12Q2565/629 C12Q2565/501 C12Q2545/114

    Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

    Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个扩增曲线并且计算多个扩增曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数并且使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个扩增曲线中的每一个的偏移。 偏移量是第一个参数和第三个参数的函数。 此外,该方法包括通过减去偏移来调整多个放大曲线中的至少一个。

    NUCLEIC ACID ENCODING REACTIONS
    7.
    发明申请
    NUCLEIC ACID ENCODING REACTIONS 审中-公开
    核酸编码反应

    公开(公告)号:WO2012162267A3

    公开(公告)日:2014-05-15

    申请号:PCT/US2012038894

    申请日:2012-05-21

    Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

    Abstract translation: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列,一个或多个靶核苷酸序列。 在具体实施方案中,产生具有适配器(例如适合用于高通量DNA测序)的核酸片段。 在其它实施方案中,关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中,本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。

    UNIVERSAL PROBE ASSAY METHODS
    8.
    发明申请
    UNIVERSAL PROBE ASSAY METHODS 审中-公开
    通用检测方法

    公开(公告)号:WO2012054933A3

    公开(公告)日:2012-06-28

    申请号:PCT/US2011057536

    申请日:2011-10-24

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3' to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面,通过扩增靶核酸序列以产生包含靶序列的扩增产物,与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列,从而产生扩增产物; 并且将第一检测探针与扩增产物杂交,所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段,从而产生标记的扩增产物 。

    SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL

    公开(公告)号:SG10201807867XA

    公开(公告)日:2018-10-30

    申请号:SG10201807867X

    申请日:2014-03-14

    Applicant: FLUIDIGM CORP

    Inventor: JONES ROBERT C

    Abstract: SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The kits, reagents, and methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids ( e.g., RNA) and proteins. FIG. 1

Patent Agency Ranking