公开(公告)号：EA020593B1

公开(公告)日：2014-12-30

申请号：EA201170933

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

IPC: C12Q1/68 ,  C12N15/11

Abstract: Изобретениеотноситсяк способаманализагеномнойДНКи/илиРНКизнебольшихобразцовилидажеединичныхклеток. СпособыанализагеномнойДНКмогутвключатьполногеномнуюамплификацию (WGA) споследующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней. СпособыанализаРНКмогутвключатьобратнуютранскрипциюжелательнойРНКс последующейпреамплификациейи амплификациейвыбранныхнуклеиновыхкислот-мишеней.

公开(公告)号：CA2734868A1

公开(公告)日：2010-03-11

申请号：CA2734868

申请日：2009-08-26

Applicant: FLUIDIGM CORP

Inventor： MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  UNGER MARC ,  ZIMMERMANN BERNHARD G

IPC: C12Q1/68 ,  C12N15/11

Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.

公开(公告)号：EP2569453A4

公开(公告)日：2013-11-20

申请号：EP11781412

申请日：2011-05-16

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  ZIMMERMANN BERNHARD

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12P19/34 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2600/118 ,  C12Q2565/113 ,  C12Q2527/125 ,  C12Q2525/204 ,  C12Q2525/307 ,  C12Q2525/15 ,  C12Q2521/507

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

Abstract translation: 本发明提供了选择性富集生物样品的方法，所述生物样品用于短核酸，例如母体样品中的胎儿DNA或来自癌症患者的生物样品中的凋亡DNA，并随后分析短核酸的基因型，突变和/ 或非整倍体。

公开(公告)号：EP2379753A4

公开(公告)日：2012-05-30

申请号：EP10732056

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

公开(公告)号：SG185544A1

公开(公告)日：2012-12-28

申请号：SG2012083432

申请日：2011-05-16

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  MIR ALAIN ,  RAMAKRISHNAN RAMESH ,  ZIMMERMANN BERNHARD

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

公开(公告)号：SG183029A1

公开(公告)日：2012-08-30

申请号：SG2012052031

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Abstract: SINGLE-CELL NUCLEIC ACID ANALYSISThe present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.Fig. 1

公开(公告)号：SG172965A1

公开(公告)日：2011-08-29

申请号：SG2011050739

申请日：2010-01-13

Applicant: FLUIDIGM CORP

Inventor： HAMILTON AMY ,  LIN MIN ,  MIR ALAIN ,  PIEPRZYK MARTIN

Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.