우수한 육질을 가진 돼지를 선별할 수 있는 바이오 마커 및상기 마커를 이용한 돼지의 육질평가방법
    21.
    发明公开
    우수한 육질을 가진 돼지를 선별할 수 있는 바이오 마커 및상기 마커를 이용한 돼지의 육질평가방법 失效
    用于选择具有良好水平的猪的生物标志物和使用其的高速估值方法

    公开(公告)号:KR1020100053341A

    公开(公告)日:2010-05-20

    申请号:KR1020080112406

    申请日:2008-11-12

    Abstract: PURPOSE: A method for determining pork meat quality using a biomarker which is able to select pork of high quality is provided to reduce production cost by selecting and raising pork of high quality and to save time and cost for quality test. CONSTITUTION: A biomarker which is able to select pork having high quality is creatine and creatine kinase. A method for developing the biomarker comprises: a step of extracting total protein from pork loin; a step of dissolving and extracting protein powder and centrifuging; a step of collecting supernatant and performing electrophoresis; a step of dyeing with silver and comparing protein expression; a step of isolating protein spot from the two-dimensional gel and performing hydrolysis; a step of analyzing proteome profiling from pork muscle between high pH and low pH groups; a step of analyzing serum sample of the high pH and low pH groups with 1H-NMR; and a step of confirming that the metabolite compositions are different between the groups.

    Abstract translation: 目的:提供一种能够选择高品质猪肉的生物标志物来确定猪肉质量的方法,通过选择和提高高品质猪肉来降低生产成本,节省质量测试的时间和成本。 构成:能够选择高品质猪肉的生物标志物是肌酸和肌酸激酶。 一种用于开发生物标志物的方法包括:从猪腰提取总蛋白质的步骤; 溶解和提取蛋白粉并离心分离的步骤; 收集上清液并进行电泳的步骤; 用银染色并比较​​蛋白质表达的步骤; 从二维凝胶中分离蛋白点并进行水解的步骤; 从高pH和低pH组之间分析猪肉蛋白质组图谱的步骤; 用1H-NMR分析高pH和低pH组的血清样品的步骤; 以及确认各组之间的代谢物组成不同的步骤。

    돼지의 성장, 육질 및 번식 형질과 관련된 유전자를 조기에진단할 수 있는 cDNA chip 과 그 개발방법 및상기 칩을 이용한 성장, 육질 및 번식 형질이 우수한 종돈선별방법

    公开(公告)号:KR1020090065412A

    公开(公告)日:2009-06-22

    申请号:KR1020080046982

    申请日:2008-05-21

    CPC classification number: C12Q1/6888 C12Q2600/124

    Abstract: A cDNA chip which early diagnoses a gene related to the growth, meat quality and breeding character of pig is provided to analyze the massive gene source of pig at once. A method for producing a cDNA chip which diagnoses a gene related to the growth, meat quality and breeding character of pig comprises: a step of preparing tissue sample from a tissue and blood of pig; a step of isolating RNA from the tissue sample; a step of producing cDNA library with 4,172 ESTs cloned from RNA; a step of producing a 4,200 cDNA chips containing 28 gene of beta-actin(Acc. No. DQ845171) and 18S(Acc. No. AF102857); and a step of mixing 254 gene having the significance in growth, meat quality and breeding character and 10 positive control genes to produce 264 cDNA chips.

    Abstract translation: 提供早期诊断与猪的生长,肉质和繁殖特性相关的基因的cDNA芯片,用于一次性分析猪的大量基因来源。 一种用于诊断与猪的生长,肉质和育种特性相关的基因的cDNA芯片的方法,包括:从猪的组织和血液制备组织样品的步骤; 从组织样品中分离RNA的步骤; 从RNA中克隆出4,172个EST的cDNA文库生成步骤; 生成含有β-肌动蛋白28基因(Acc。No.DD845171)和18S(Acc.No.AF102857)的4,200个cDNA芯片的步骤; 以及混合具有生长,肉质和育种特征的10种阳性对照基因的254基因的混合物的步骤,生成264个cDNA片段。

    유전자의 발현프로필을 이용한 돼지의 산자수 예측방법
    29.
    发明公开
    유전자의 발현프로필을 이용한 돼지의 산자수 예측방법 有权
    使用基因表达谱的SWINE FECUNDITY的预测方法

    公开(公告)号:KR1020160049177A

    公开(公告)日:2016-05-09

    申请号:KR1020140145448

    申请日:2014-10-24

    CPC classification number: C12Q1/6813 C12Q2600/124

    Abstract: 본발명은 DEG를활용한돼지의산자수예측방법에관한것이다. 본발명에서는산자수가우수한흑돼지품종을유전자원으로개량하기위해산자수가우수한모돈과산자수가열등한모돈의태반으로부터 RNA을분석하여 DEG(Differentially Expressed Gene)를얻고, 이들유전자와산자수와의연계성을정립하여산자수가우수한흑돼지계통조성을위한진단기술을제공한다.

    Abstract translation: 本发明涉及通过应用差异表达基因(DEG)来估计猪繁殖力的方法。 为了改善遗传资源,显示出优异繁殖力的黑猪品种,对来自较少繁殖母猪和高度繁殖母猪的胎盘的RNA进行分析,以获得DEG,并且 确定了基因和繁殖力,使得可以提供一种诊断技术,用于建立显示出优异繁殖力的黑猪谱系。

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