Abstract:
본 발명은 멍게 물렁증을 일으키는 원생동물에 관한 것이다. 또한, 본 발명은 상기 원생동물의 18S rDNA의 595 bp 단편, 이 단편의 뉴클레오티드 서열의 일부를 이용하는 멍게 물렁증 PCR 진단법 및 인시추 하이브리드화 진단법, 및 PCR 진단법 및 인시추 하이브리드화 진단법에 이용되는 프라이머 및 프로브에 관한 것이다. 멍게 물렁증, PCR 진단, 인시추 하이브리드화 진단
Abstract:
A method for controlling aquatic fungi and fish pathogenic bacteria of cultivated fish is provided to improve productivity of the cultivated fish by effectively controlling the aquatic fungi and fish pathogenic bacteria using a proper amount of an extract of Opuntia ficus-indica var. saboten Makino and provide safe aquatic products to consumers by using environment-friendly and harmless Opuntia ficus-indica var. saboten Makino extract. To exterminate aquatic fungi of fish eggs, the fish eggs are treated with 50-80 ppm of a hydrolysis fraction of Opuntia ficus-indica var. saboten Makino for 30 minutes every 3 days until the fish eggs become germinated egg. To exterminate aquatic fungi of fish, the fish is treated with 200 ppm of the hydrolysis fraction of Opuntia ficus-indica var. saboten Makino for 2 hours per day one or two times. To treat and prevent fish pathogenic bacteria of cultivated fish, 0.1% hydrolysis fraction of Opuntia ficus-indica var. saboten Makino is absorbed into an expansion pellet and the expansion pellet is administered into the cultivated fish for 4 weeks.
Abstract:
A gene of a parasite causing death of Halocynthia roretzi and a method for diagnosing a parasite are provided to obtain a living organism that causes perishing of Halocynthia roretzi, and to diagnose a parasite by using a part of 18S rDNA fraction causing perishing of Halocynthia roretzi. A method for diagnosing a parasite includes the steps of: separating the entire nucleic acid from Halocynthia roretzi; performing PCR reaction by having the separated nucleic acid as a template and having a front primer and a reverse primer having a partial sequence of 18SrDNA fraction having a nucleotide sequence of sequence number 1 as a primer pair; and checking the size of the product of the PCR reaction by gel electrophoresis and comparing the size of the aforesaids product with the size of the product that is anticipated from the used primer pair.