公开(公告)号：KR101491803B1

公开(公告)日：2015-02-16

申请号：KR1020130108359

申请日：2013-09-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정병열 ,  김애란 ,  정석찬 ,  이지연 ,  박혜연 ,  서병주

IPC: C12N1/20 ,  A61K35/74 ,  A61K39/102 ,  C12Q1/02

CPC classification number: C12N1/20 ,  A61K35/74 ,  A61K39/102 ,  C12Q1/02

Abstract: 본 발명은 돼지 흉막폐렴에 대한 예방 효과를 갖는 신규한 액티노바실러스 플로르뉴모니아 혈청형 2번 균주 KACC 91766P, 상기 균주를 포함하는 돼지 흉막폐렴 진단용 조성물 및 상기 균주를 포함하는 돼지 흉막폐렴 예방용 백신 조성물에 관한 것이다.

Abstract translation: 本发明涉及一种具有预防猪胸膜肺炎的效果的新型放线杆菌胸膜血管炎血清型2株KACC 91766P， 用于诊断含有该菌株的猪胸膜肺炎的组合物; 以及用于预防含有该菌株的猪胸膜肺炎的疫苗组合物。

公开(公告)号：KR101149875B1

公开(公告)日：2012-06-27

申请号：KR1020090101085

申请日：2009-10-23

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 강민수 ,  권용국 ,  정병열 ,  김애란 ,  임춘태 ,  이강무 ,  안병기 ,  권준헌

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: Y02A50/451

Abstract: PURPOSE: A method for detecting Salmonella gallinarum or probiotics for Salmonella gallinarum vaccine is provided to quickly detect Salmonella gallinarum. CONSTITUTION: A method for detecting Salmonella gallinarum or probitocs Salmonella gallinarum comprises: a step of preparing DNA from a biological sample; a step of performing PCR using the DNA as a temperate and primer; and a step of analyzing DNA fragment through from PCR product.

公开(公告)号：KR101149872B1

公开(公告)日：2012-06-27

申请号：KR1020090028760

申请日：2009-04-03

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  김애란 ,  임숙경 ,  조윤상 ,  임춘태 ,  윤민 ,  정석찬

IPC: A61K39/112 ,  C12N5/07 ,  A61K39/10 ,  A61P37/00

Abstract: 본 발명은 병원균의 약독화 방법에 관한 것으로, 보다 자세하게는 병원균과 호중구를 공동 배양함으로써 상기 병원균을 약독화시키는 방법, 이렇게 약독화된 병원균 변이주 및 상기 변이주를 함유하는 백신 조성물에 관한 것이다.
병원균, 살모넬라, 호중구, 약독화

公开(公告)号：KR1020100110434A

公开(公告)日：2010-10-13

申请号：KR1020090028760

申请日：2009-04-03

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 이희수 ,  김애란 ,  임숙경 ,  조윤상 ,  임춘태 ,  윤민 ,  정석찬

IPC: A61K39/112 ,  C12N5/07 ,  A61K39/10 ,  A61P37/00

CPC classification number: A61K39/0275 ,  A61K2039/522

Abstract: PURPOSE: A method for attenuating pathogen by co-culture of pathogen and neutrophil is provided to attenuate without high technology and safely apply to human body. CONSTITUTION: A pathogen is attenuated by co-culture of pathogen and neutrophils. The neutrophil is isolated from poultry, pig, lamb, horse, cat, dog or human. A method for co-culture of pathogen and neutrophil comprises: a step of co-culture of neutrophili and pathogen for 10 minutes to two hours; a step of killing pathogen; and a step of collecting and dissolving neutrophil. The attenuated pathogen is ST31-N5th-1(KACC 91447P) or ST31-N20th-B10th-12(KACC 91448P). A vaccine composition contains attenuated pathogenic mutant.

Abstract translation: 目的：通过病原体和嗜中性粒细胞的共培养来减轻病原体的方法，无需高技术的减毒，安全应用于人体。 构成：通过病原体和嗜中性粒细胞的共培养来减轻病原体。 嗜中性粒细胞与家禽，猪，羊，马，猫，狗或人类分离。 病原体和嗜中性粒细胞的共培养方法包括：将嗜中性粒细胞和病原体共培养10分钟至2小时的步骤; 杀死病原体的一个步骤; 和收集和溶解嗜中性粒细胞的步骤。 减毒病原体是ST31-N5th-1（KACC91447P）或ST31-N20th-B10th-12（KACC91448P）。 疫苗组合物含有减毒致病突变体。

公开(公告)号：KR1020100006868A

公开(公告)日：2010-01-22

申请号：KR1020080067171

申请日：2008-07-10

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정병열 ,  이경기 ,  박최규 ,  엄재구 ,  최정수 ,  송윤경 ,  강민수 ,  김애란 ,  이경우 ,  문운경 ,  권준헌 ,  권창희

IPC: G01N30/02 ,  G01N30/00

Abstract: PURPOSE: A diagnostic kit for rapid detection of rabies antibodies is provided to rapidly detect the existence according to rabies vaccination within 20 minutes without expensive equipment and to reduce the generation of rabies. CONSTITUTION: A diagnostic kit for rapid detection of rabies antibodies using immunochromatography has a nitrocellulose membrane(30) containing a test line(40) and a control line(50) on a backing card(70). A conjugate pad is overlapped at one side of the nitrocellulose membrane. A sample pad(10) is overlapped on the conjugate pad. An adsorbent pad(60) absorbing blood serum in which the reaction is completed on a backing card is installed at the other side of the nitrocellulose membrane.

Abstract translation: 目的：提供用于快速检测狂犬病抗体的诊断试剂盒，用于在20分钟内快速检测狂犬病疫苗的存在，无需昂贵的设备，并减少狂犬病的产生。 构成：用于使用免疫层析法快速检测狂犬病抗体的诊断试剂盒具有在背板（70）上含有测试线（40）和对照线（50）的硝酸纤维素膜（30）。 缀合垫在硝酸纤维素膜的一侧重叠。 样品垫（10）重叠在共轭垫上。 在硝酸纤维素膜的另一侧安装吸收血清的吸附垫（60），其中在背衬卡上完成反应。

公开(公告)号：KR1020090114665A

公开(公告)日：2009-11-04

申请号：KR1020080040422

申请日：2008-04-30

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 정병열 ,  강민수 ,  김애란 ,  박최규 ,  정석찬 ,  권준헌

IPC: G01N33/48 ,  G01N33/52 ,  G01N33/543

Abstract: PURPOSE: A diagnostic latex liquid and a simple method for diagnosing laptospira are provided to quickly confirm infection of laptospira and minimize the infection. CONSTITUTION: A diagnostic latex liquid using laptospira serum sejroe is prepared by crushing the laptospira serum sejroe by ultrasonic wave and adhering to latex bead. An antigen used for latex diagnosis is laptospira serum sejroe. The crushing is performed at 10-60 KHz for 10 to 30 minutes. A method for diagnosing laptospira comprises: a step of mixing same amount of test serum with 30ul of latex liquid on glass plate; a step of shaking the glass plate and read formation of aggregation; and a step of diagnosing that the formation of specific aggregation is laptospira positive and no aggregation is laptospira negative.

Abstract translation: 目的：提供诊断乳液液体和简单的诊断Laptospira方法，以快速确认laptospira感染并尽量减少感染。 构成：使用laptospira血清sejroe的诊断胶乳液通过超声波粉碎laptospira血清sejroe并粘附到乳胶珠制备。 用于乳胶诊断的抗原是laptospira血清sejroe。 破碎在10-60 KHz进行10〜30分钟。 一种诊断眼线笔的方法包括：将相同量的血清与30ul胶乳液混合在玻璃板上的步骤; 摇晃玻璃板并阅读聚集的步骤; 诊断特定聚集体形成为laptospira阳性并且没有聚集是laptospira阴性的步骤。