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公开(公告)号：KR1019950018483A
公开(公告)日：1995-07-22
申请号：KR1019930028158
申请日：1993-12-17
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 특정 DNA서열을 증폭할 수 있는 특성을 이용하여 감자엽권, 감자X, Y 바이러스 및 흑조위축병 바이러스를 특이적으로 검정해낼 수 있는 핵산증식용 프라이며 염기서열 및 이를 이용한 작물의 바이러스 이병 여부의 진단 방법을 제공한다.
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公开(公告)号：KR1020150052904A
公开(公告)日：2015-05-15
申请号：KR1020130134133
申请日：2013-11-06
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은고도불포화지방산생합성에관한것으로, 보다상세하게는 pAO815 벡터를이용한세 개의유전자와,가동시에발현되도록다중발현벡터를만들어진핵세포의단백질의생산에이용되는유용한시스템인메탄이용효모인에형질전환함으로써효모가생산해내는기질 linoleic acid (LA, C18:2 n-6) 또는 a-linolenic acid (ALA, C18:3 n-3)로부터고도불포화지방산인, gamma-linolenic acid (GLA, C18:3 n-6), stearidonic acid (STA, C18:4 n-3), di-homo-linolenic acid (DGLA, 20:3n-6), eicosatetraenoic acid (ETA, 20:4n-3), arachidonic acid (ARA; 20:4n-6), eicosapentaenoic acid (EPA, 20:5n-3), docosatetraenoic acid (DTA, 22:4n-6) 또는 docosapentaenoic acid (DPA, 222:5n-3)로의전환을가스크로마토그래피로분석하여 3개유전자의동시발현기능을검정하였으므로, 이를고도불포화지방산생산자원으로유용하게사용할수 있다.
Abstract translation: 本发明涉及多不饱和脂肪酸的生物合成，更具体地说，使用pAO815载体共同表达McD6DES，AsELOVL5和PtD5DES的三个基因的多重表达载体，并将载体转化为巴斯德毕赤酵母，其使用甲烷为酵母 用于生产真核细胞蛋白质的可用系统，由于分析了由诸如亚油酸（LA，C18：2 n-6）的酵母产生的细胞外基质的转化，有价值地用作多不饱和脂肪酸生产源， 或α-亚麻酸（ALA，C18：3n-3）与多亚不饱和脂肪酸如γ-亚麻酸（GLA，C18：3n-6），十八碳四烯酸（STA，C18：4n-3） 二 - 十二碳烯酸（DGLA，20：3n-6），二十碳四烯酸（ETA，20：4n-3），花生四烯酸（ARA; 20：4n-6），二十碳五烯酸 ），二十二碳四烯酸（DTA，22：4n-6）或二十二碳五烯酸（DPA，222：5n-3），通过气相色谱法检测共表达 功能的三个基因。
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公开(公告)号：KR1020110063923A
公开(公告)日：2011-06-15
申请号：KR1020090120295
申请日：2009-12-07
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A Sparus aurata-derived delta-6 desaturase protein, SaD6DES is provided to develop oil plants which produce functional gamma-linolenic acid and stearidonic acid. CONSTITUTION: A Sparus aurata-derived delta-6 desaturase protein has an amino acid of sequence number 2. A gene encoding the protein contains a base sequence of sequence number 1. A recombinant vector, pYES2-SaD6DES, contains the gene. A method for preparing gamma-linolenic acid or stearidonic acid in host cells comprises a step of transforming a host cell with the recombinant vector. The host cell is yeast. A composition for producing gamma-linolenic acid or stearidonic acid contains the gene.
Abstract translation: 目的：提供一种来源于蓝莓的Delta-6去饱和酶蛋白，SaD6DES用于开发产生功能性γ-亚麻酸和十八碳四烯酸的油植物。 构成：来自天竺葵的δ-6去饱和酶蛋白具有序列号2的氨基酸。编码蛋白质的基因含有序列号1的碱基序列。重组载体pYES2-SaD6DES含有该基因。 在宿主细胞中制备γ-亚麻酸或十八碳四烯酸的方法包括用重组载体转化宿主细胞的步骤。 宿主细胞是酵母。 用于产生γ-亚麻酸或十八碳四烯酸的组合物含有该基因。
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25.发明公开
公开(公告)号：KR1020080075421A
公开(公告)日：2008-08-18
申请号：KR1020070014589
申请日：2007-02-12
Applicant: 대한민국(농촌진흥청장)
Abstract: A fusion protein of Escherichia coli enterotoxin protein and a neuraminidase epitope of Newcastle disease virus is provided to increase immunity against Newcastle disease virus in poultry, so that the protein is useful for prevention of Newcastle disease virus. A fusion protein of E. coli heat-labile enterotoxin subunit B and a neuraminidase epitope of Newcastle disease virus has the amino acid sequence of SEQ ID NO:1. A polynucleotide encoding the fusion protein has the nucleotide sequence of SEQ ID NO:2. An expression vector pLD-CtV-HN contains the polynucleotide of SEQ ID NO:2. A vaccine composition for preventing Newcastle disease virus contains the fusion protein as an effective ingredient. The immunity against Newcastle disease virus in poultry is increased by administering the fusion protein or a transgenic plant containing the expression vector pLD-CtV-HN into poultry.
Abstract translation: 提供大肠杆菌肠毒素蛋白的融合蛋白和新城疫病毒的神经氨酸酶表位，以增加对家禽新城疫病毒的免疫力，使得该蛋白质可用于预防新城疫病毒。 大肠杆菌热不稳定肠毒素亚基B和新城疫病毒的神经氨酸酶表位的融合蛋白具有SEQ ID NO：1的氨基酸序列。 编码融合蛋白的多核苷酸具有SEQ ID NO：2的核苷酸序列。 表达载体pLD-CtV-HN含有SEQ ID NO：2的多核苷酸。 用于预防新城疫病毒的疫苗组合物含有融合蛋白作为有效成分。 通过将融合蛋白或含有表达载体pLD-CtV-HN的转基因植物施用于家禽来增加家禽中新城疫病毒的免疫力。
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Patent Agency Ranking
公开(公告)号：KR1019970011310B1
公开(公告)日：1997-07-09
申请号：KR1019930028158
申请日：1993-12-17
Applicant: 대한민국(농촌진흥청장)
Abstract: The diagnostic method is disclosed which makes it possible to determine the presence of a virus infection in a crop by using the primer for nucleic acid amplification. The method provides the primer which can assay virus related diseases specifically, which is used to characteristic amplifying a specific DNA sequence. Included in the invention are the extraction of virus RNA from virus infected plant, the synthesis of cDNA of single strand from the virus RNA by means of reverse transcription reaction with using the primer, polymerase chain reaction(PCR) by using the primer for the template of the cDNA, and the certification of a virus specific DNA fragment band by agarose gel electrophoresis of the PCR product.
Abstract translation: 公开了诊断方法，其可以通过使用用于核酸扩增的引物来确定作物中病毒感染的存在。 该方法提供了可以特异性测定病毒相关疾病的引物，其用于特异性扩增特异性DNA序列。 本发明包括从病毒感染的植物中提取病毒RNA，通过使用引物的逆转录反应合成来自病毒RNA的单链cDNA，使用引物作为模板进行聚合酶链式反应（PCR） 的cDNA，通过PCR产物的琼脂糖凝胶电泳鉴定病毒特异性DNA片段。
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公开(公告)号：KR102215717B1
公开(公告)日：2021-02-22
申请号：KR1020190073039
申请日：2019-06-19
Applicant: 대한민국(농촌진흥청장) , 명지대학교 산학협력단
IPC: C12Q1/6895
Abstract: 본발명의쑥 품종판별용 SSR(Simple Sequence Repeat) 프라이머세트, 상기프라이머세트를포함하는쑥 품종판별용키트및 이를이용한쑥 품종판별방법에관한것으로, 쑥의유전적인식별을통한품질규격과유통질서의확립을위한체계적인품종관리에활용가능하다.
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公开(公告)号：KR101549147B1
公开(公告)日：2015-09-02
申请号：KR1020130134133
申请日：2013-11-06
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은고도불포화지방산생합성에관한것으로, 보다상세하게는 pAO815 벡터를이용한세 개의유전자와,가동시에발현되도록다중발현벡터를만들어진핵세포의단백질의생산에이용되는유용한시스템인메탄이용효모인에형질전환함으로써효모가생산해내는기질 linoleic acid (LA, C18:2 n-6) 또는 a-linolenic acid (ALA, C18:3 n-3)로부터고도불포화지방산인, gamma-linolenic acid (GLA, C18:3 n-6), stearidonic acid (STA, C18:4 n-3), di-homo-linolenic acid (DGLA, 20:3n-6), eicosatetraenoic acid (ETA, 20:4n-3), arachidonic acid (ARA; 20:4n-6), eicosapentaenoic acid (EPA, 20:5n-3), docosatetraenoic acid (DTA, 22:4n-6) 또는 docosapentaenoic acid (DPA, 222:5n-3)로의전환을가스크로마토그래피로분석하여 3개유전자의동시발현기능을검정하였으므로, 이를고도불포화지방산생산자원으로유용하게사용할수 있다.
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公开(公告)号：KR1020140066881A
公开(公告)日：2014-06-03
申请号：KR1020120133513
申请日：2012-11-23
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/93 , C12N15/81 , C12N15/8247 , C12P7/6427 , C12Y602/01003
Abstract: The present invention relates to a fatty acid chain elongase protein derived from Muraenesox cinereus, and to a method for producing an unsaturated fatty acid selected from a group consisting of a di-homo-gamma linolenic acid (DHLA), a docosatetraenoic acid (DTA), and a docosapentaenoic acid (DPA), using the same.
Abstract translation: 本发明涉及来自Muraenesox cinereus的脂肪酸链延长酶蛋白质，以及选自二 - 高-γ亚麻酸（DHLA），二十二碳四烯酸（DTA））的不饱和脂肪酸的制备方法， ，和二十二碳五烯酸（DPA），使用它们。
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公开(公告)号：KR1020130013453A
公开(公告)日：2013-02-06
申请号：KR1020110075108
申请日：2011-07-28
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A novel delta-6 desaturase gene derived from Muraenesox cinereus and a transformant containing the same are provided to obtain an oil seed which produces functional gamma-linolenic acid. CONSTITUTION: A gene encoding delta-6 desaturase derived from Muraenesox cinereus has a nucleic acid sequence of sequence number 1. A method for preparing a transformant comprises: a step of preparing an expression vector containing DNA sequence encoding delta-6 desaturase; a step of introducing the expression vector into a host cell; and a step of selecting a transfornmant containing the expression vector. The host cell is yeast(Saccharomyces cerevisiae). [Reference numerals] (AA) LA(substrate); (BB) GLA(product)
Abstract translation: 目的：提供一种来自Muraenesox cinereus的新型δ-6去饱和酶基因和含有它的转化体，以获得产生功能性γ-亚麻酸的油籽。 构成：编码来自Muraenesox cinereus的δ-6去饱和酶的基因具有序列号1的核酸序列。一种制备转化体的方法，包括：制备含有编码δ-6去饱和酶的DNA序列的表达载体的步骤; 将表达载体导入宿主细胞的步骤; 以及选择含有表达载体的转录因子的步骤。 宿主细胞是酵母（酿酒酵母（Saccharomyces cerevisiae））。 （标号）（AA）LA（底物）; （BB）GLA（产品）
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