公开(公告)号：KR1020130001509A

公开(公告)日：2013-01-04

申请号：KR1020110062312

申请日：2011-06-27

Applicant: 삼성전자주식회사

Inventor： 김재영 ,  박성민 ,  박영경 ,  유병조 ,  박재찬 ,  조화영 ,  구현민

IPC: C12N15/53 ,  C12N15/63 ,  C12P7/52

CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y203/01008

Abstract: PURPOSE: A recombinant microorganism which produces 3-hydroxypropionic acid(3-HP) using a malonic semialdehyde reduction pathway is provided to remarkably enhance 3-HP productivity by gene manipulation. CONSTITUTION: A recombinant microorganism for producing 3-hydroxypropionic acid has a pathway for sequentially producing pyruvate, acetyl Co, malonyl-CoA, and malonic semialdehyde. The microorganism includes Escherichia sp., Saccharomyces sp., or Kluyveromyces sp. A gene encoding lactate dehydrogenase is IdhA or a homolog or a variant thereof. A gene encoding phosphotransacetylase is pta or a homolog or a variant thereof.

Abstract translation: 目的：提供使用丙二醛还原途径产生3-羟基丙酸（3-HP）的重组微生物，通过基因操作显着提高3-HP生产力。 构成：用于产生3-羟基丙酸的重组微生物具有顺序产生丙酮酸，乙酰辅酶A，丙二酰辅酶A和丙二醛半醛的途径。 微生物包括大肠杆菌，酵母属或克鲁维酵母属。 编码乳酸脱氢酶的基因是IdhA或其同源物或其变体。 编码磷酸转乙酰酶的基因是pta或其同系物或其变体。

公开(公告)号：KR1020130001121A

公开(公告)日：2013-01-03

申请号：KR1020120061819

申请日：2012-06-08

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N1/19 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

Abstract: PURPOSE: A modified microorganism for producing lactic acids and a method for producing lactic acids are provided to produce lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids with high efficiency has LDH activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, and Rattus norvegicus. The modified microorganism is yeast or bacteria. The modified microorganism is E.coli or Kluyveromyces marxianus. The modified microorganism produces lactic acids with 12.2% or more of glucose. An expression vector for producing the modified microorganism comprises: a replication origin; a promoter; a polynucleotide; and a terminator. A method for producing lactic acids comprises: a step of culturing the modified microorganism in a medium containing glucose; and a step of collecting lactic acids from the culture.

Abstract translation: 目的：提供用于生产乳酸的改性微生物和生产乳酸的方法，以在酸性条件下高效生产乳酸。 构成：用于高效生产乳酸的改良微生物具有日本芍药，鸟腥草，Tur ps属，and and属的LDH活性。 修饰的微生物是酵母或细菌。 修饰的微生物是大肠杆菌或马克斯克鲁维酵母（Kluyveromyces marxianus）。 经修饰的微生物产生具有12.2％或更多葡萄糖的乳酸。 用于产生修饰微生物的表达载体包括：复制起点; 启动子 多核苷酸; 和终结者。 制备乳酸的方法包括：在含有葡萄糖的培养基中培养经修饰的微生物的步骤; 以及从培养物中收集乳酸的步骤。

公开(公告)号：KR1020130001103A

公开(公告)日：2013-01-03

申请号：KR1020110139520

申请日：2011-12-21

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 정순천 ,  구현민 ,  김재영 ,  김지은 ,  김진우 ,  박영경 ,  이소영 ,  조화영 ,  권대혁 ,  박재찬

IPC: C12N15/63 ,  C12N1/21 ,  C12N15/53 ,  C12P7/56

Abstract: PURPOSE: A modified microorganism for producing lactic acids is provided to prepare lactic acids with high efficiency under an acidic condition. CONSTITUTION: A modified microorganism for producing lactic acids has a lactate dehydrogenase(LDH) activity of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus. The modified microorganism is Escherichia sp. or Kluyveromyces sp. The modified microorganism produces lactic acids with 34% or more of glucose. An expression vector contains: a replication origin for constructing the modified microroganisms; a promoter; a polynucleotide coding LDH activation of one or more species selected from the group consisting of Pelodiscus sinensis japonicus, Ornithorhynchus anatinus, Tursiops truncates, or Rattus norvegicus; and a terminator. The replication origin is ARS/CEN replication origin.

Abstract translation: 目的：提供一种用于生产乳酸的改性微生物，用于在酸性条件下高效制备乳酸。 构成：用于生产乳酸的改良微生物具有日本芍药（Ornisorhynchus anatinus，Tursiops truncates）或褐藻（Rattus norvegicus）的乳酸脱氢酶（LDH）活性。 修饰的微生物是大肠杆菌属 或克鲁维酵母属 经修饰的微生物产生具有34％以上葡萄糖的乳酸。 表达载体包含：用于构建修饰的微结构的复制起点; 启动子 编码一种或多种选自以下的物质的LDH活化的多核苷酸：日本Pe us，鸟，us us us，，，;;;;;;;;;;;;;;;;;; 和终结者。 复制起点是ARS / CEN复制来源。

公开(公告)号：KR1020130000883A

公开(公告)日：2013-01-03

申请号：KR1020110061677

申请日：2011-06-24

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 구현민 ,  박성민 ,  유병조 ,  이기성 ,  박재찬 ,  권대혁

IPC: C12N15/70 ,  C12N15/81 ,  C12N15/67

CPC classification number: C12N15/815 ,  C12P21/00 ,  C12P21/02

Abstract: PURPOSE: An expression vector which overexpresses target proteins, and a method for producing the target protein are provided to obtain the proteins in K. marxianus. CONSTITUTION: An expression vector contains: a replication origin; a CYC promoter, a TEF promoter, a GPD promoter, or an ADH promoter; and a terminator. The CYC promoter contains a sequence of sequence number 1 or a sequence having 70% or more sequence homology with the sequence of sequence number 1. The TEF promoter has a sequence of sequence number 2 or a sequence with 70% or more sequence homology with the sequence of sequence number 2. GPD promoter contains a sequence of sequence number 3 or a sequence having 70% or more homology with the sequence of sequence number 3. The ADH promoter has a sequence of sequence number 4 or a sequence having 70% or more sequence homology with the sequence of sequence number 4.

Abstract translation: 目的：提供过表达靶蛋白的表达载体和产生靶蛋白的方法，以获得马克斯马氏霉属中的蛋白质。 构成：表达载体包含：复制起点; CYC启动子，TEF启动子，GPD启动子或ADH启动子; 和终结者。 CYC启动子含有序列号1的序列或与序列号1的序列具有70％以上序列同源性的序列.TEF启动子具有序列号2的序列或具有70％以上序列同源性的序列 序列号2的序列.GPD启动子含有序列号3的序列或与序列号3的序列具有70％以上同源性的序列.ADH启动子具有序列号4的序列或70％以上的序列 与序列号4的序列同源性。

公开(公告)号：KR1020130000640A

公开(公告)日：2013-01-03

申请号：KR1020110061290

申请日：2011-06-23

Applicant: 삼성전자주식회사

Inventor： 조화영 ,  유병조 ,  박재찬 ,  박성민 ,  구현민 ,  이주영

IPC: C12Q1/04 ,  C12N1/20 ,  C12N1/14

CPC classification number: G01N21/80 ,  C12Q1/045 ,  Y02A50/451

Abstract: PURPOSE: A high throughput screening system and method using a pH indicator are provided to accurately, quickly, and easily screen strains which highly produce acids. CONSTITUTION: A system for screening acid-producing stains contains the acid-producing strains, a medium, two or more pH indicators. The acid includes citric acid, itaconic acid, succinic acid, fumaric acid, glycolic acid, pyruvic acid, acetic acid, glutamic acid, malic acid, maleic acid, 3-hydroxypropionic acid(3-HP), butyric acid, or gluconic acid. The strains are Bacillus sp., Streptococcus sp., Streptomyces sp., Staphylococcus sp., Enterococcus sp., Lactobacillus sp., Lactococcus sp., Clostridium sp., Geobacillus sp., Escherichia. Coli sp., Pseudomonas sp., Salmonella sp., Campylobacter sp., Helicobacter sp., Flavobactenum sp., Fusobacterium sp., llyobacter sp., Neisseria sp., Candida sp., Hansenula sp., Kluyveromyces sp., Pichia sp., Saccharomyces sp., Schizosaccharomyces sp., or Yarrowta sp.

Abstract translation: 目的：提供高通量筛选系统和使用pH指示剂的方法来准确，快速，容易地筛选高度产生酸的菌株。 构成：用于筛选产酸污渍的系统包含酸生产菌株，中等，两种或更多种pH指示剂。 酸包括柠檬酸，衣康酸，琥珀酸，富马酸，乙醇酸，丙酮酸，乙酸，谷氨酸，苹果酸，马来酸，3-羟基丙酸（3-HP），丁酸或葡萄糖酸。 菌株是芽孢杆菌属，链球菌属，链霉菌属，葡萄球菌属，肠球菌属，乳杆菌属，乳球菌属，梭菌属，地芽孢杆菌属，埃希氏菌属。 大肠杆菌，假单胞菌属，沙门氏菌属，弯曲菌属，幽门螺杆菌属，黄杆菌属，福氏杆菌属，丝杆菌属，奈瑟氏球菌属，假丝酵母​​属，汉逊酵母属，克鲁维酵母菌属，毕赤酵母属 ，酵母属（Saccharomyces sp。），粟酒裂殖酵母属（Schizosaccharomyces sp。）或Yarrowta sp。

公开(公告)号：KR1020120108538A

公开(公告)日：2012-10-05

申请号：KR1020110026531

申请日：2011-03-24

Applicant: 삼성전자주식회사

Inventor： 박성민 ,  김재영 ,  박재찬 ,  구현민 ,  유병조 ,  조화영 ,  박영경

IPC: C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12P7/62

CPC classification number: C12N9/0008 ,  C12N9/0036 ,  C12P7/42 ,  C12Y102/01075

Abstract: PURPOSE: A method for preparing 3-hydroxypropionic acid using a recombinant microorganism is provided to enhance the productivity by resolve oxidation-reduction unbalance. CONSTITUTION: A recombinant microorganism for preparing 3-hydroxypropionic acid(3-HP) has an activity of reducing malonic semialdehyde to 3-HP and an activity of reducing malonyl-CoA to malonic semialdehyde. The malonyl-CoA reductase(mcr) activation and malonate semialdehyde reductase(msr) activation are derived from M. sedula. A recombinant microorganism for preparing 3-HP additionally has NADPH generation.

Abstract translation: 目的：提供使用重组微生物制备3-羟基丙酸的方法，通过解决氧化还原不平衡来提高生产率。 构成：用于制备3-羟基丙酸（3-HP）的重组微生物具有将丙二醛降低至3-HP的活性，并将丙二酰辅酶A还原成丙二醛的活性。 丙二酰辅酶A还原酶（mcr）活化和丙二酸半醛还原酶（msr）活化来自M. sedula。 用于制备3-HP的重组微生物另外具有NADPH生成。

公开(公告)号：KR1020100048224A

公开(公告)日：2010-05-11

申请号：KR1020080107277

申请日：2008-10-30

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/12 ,  C12N1/16 ,  C12N15/66

CPC classification number: C12P7/08 ,  C07K14/395 ,  Y02E50/17

Abstract: PURPOSE: A gene which enhances metabolic rate of galactose is provided to enhance productivity of bioalcohol from biomass. CONSTITUTION: A gene which enhances metabolic rate of galactose is formed by losing whole or partial expression suppressing domain in a regulatory gene which suppresses expression of galactose matobolic gene. The regulatory gene is a gene encoding a TUP1 protein. A domain suppressing expression of the gene is a C-terminal repressor domain. The regulatory gene has a polynucleotide sequence of sequence number 1. The TUP1 protein has an amino acid sequence of sequence number 2. A pRS424 recombinant vector contains the of sequence number 1. A transformed recombinant microorganism is prepared using the recombinant vector. The microorganism is yeast. The recombinant microorganism is Saccharomyces cerevisiae CEN.PK2-1D/pRS424-truncated TUP1 of deposit number KCTC 11387 BP.

Abstract translation: 目的：提高提高半乳糖代谢率的基因，以提高生物质中生物醇的生产力。 构成：通过在抑制半乳糖基质的表达的调节基因中失去全部或部分表达抑制结构域，形成提高半乳糖代谢率的基因。 调节基因是编码TUP1蛋白的基因。 抑制基因表达的结构域是C末端阻遏酶结构域。 调节基因具有序列号1的多核苷酸序列.TUP1蛋白具有序列号2的氨基酸序列.PRS424重组载体含有序列号1.使用重组载体制备转化的重组微生物。 微生物是酵母。 重组微生物是保藏号为KCTC 11387BP的酿酒酵母CEN.PK2-1D / pRS424-截短的TUP1。