公开(公告)号：DE50203658D1

公开(公告)日：2005-08-25

申请号：DE50203658

申请日：2002-05-24

Applicant: LEICA MICROSYSTEMS

Inventor： ULRICH HEINRICH ,  BIRK DR ,  HAY WILLIAM ,  NISSLE HOLGER

IPC: G02B26/10 ,  G02B21/00 ,  G02B27/10 ,  G11B7/00 ,  H01L31/00

Abstract: Device for determination of the light intensity of a light beam (7) has a beam splitter (1) and a detector (11). The beam splitter divides off a measurement beam (23) from the incident beam and the ratio of the intensity of the light in the incident beam to that of the measurement beam is measured using the detector to ensure that the ratio remains constant. Beam splitter and detector form a single unit. Independent claims are also made for: (1) A microscope; (2) A microscopy method in which the intensity of the incident illumination beam is monitored.

公开(公告)号：DE10061603B4

公开(公告)日：2004-07-15

申请号：DE10061603

申请日：2000-12-11

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  ULRICH HEINRICH

IPC: G02B21/00 ,  G02B21/06

公开(公告)号：DE10228477A1

公开(公告)日：2004-01-15

申请号：DE10228477

申请日：2002-06-26

Applicant: LEICA MICROSYSTEMS

Inventor： ULRICH HEINRICH

IPC: G01J3/28 ,  G02B21/00 ,  G01J3/30

Abstract: The method involves generating reference light whose spectral composition is known with a reference light source and coupling the reference light into the detection light path of the scanning microscope (3). The scanning microscope has an objective (21) and the light is coupled in the vicinity of the focal plane of the objective. The reference light can be transported by means of an optical fiber. An independent claim is also included for the following: (a) a device for calibrating a spectral detector in a scanning microscope.

公开(公告)号：DE59906884D1

公开(公告)日：2003-10-23

申请号：DE59906884

申请日：1999-07-06

Applicant: LEICA MICROSYSTEMS

Inventor： ULRICH HEINRICH ,  BORLINGHAUS ROLF

IPC: G02B6/26 ,  G02B6/34 ,  G02B21/00 ,  G02B21/06 ,  H01S3/00

公开(公告)号：DE19842153C2

公开(公告)日：2003-07-31

申请号：DE19842153

申请日：1998-09-15

Applicant: LEICA MICROSYSTEMS

Inventor： ULRICH HEINRICH ,  ENGELHARDT JOHANN ,  HAY WILLIAM C

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/16

Abstract: The invention relates to an optical system in the ray path of a confocal fluorescence microscope, comprising at least one laser light source (1, 2), a device positioned in the illuminating/detecting beam (3, 4, 5) for separating the exciting light (8) from the fluorescent light (9), an objective (10) arranged between the device and the object (7), and a detector (11) positioned downstream of the device situated in the detecting beam (5). The aim of the invention is to increase the fluorescence yield of the system while retaining its compact structure. To this end the separating device comprises a mirror (13) which is dimensioned and positioned in the illuminating and/or detecting beam (3, 4, 5) in such a way that for the dark-field illumination of the object (7) it reflects the non-expanded exciting beam arriving from the laser light source (1, 2) into the objective (10) and permits the transit of the fluorescence light (9) arriving from the object (7) in the direction of the detector (11) with full numerical aperture, the fluorescent light beam being reduced by the mirror (13) cross-section active in the detecting beam (5).

公开(公告)号：DE59707665D1

公开(公告)日：2002-08-08

申请号：DE59707665

申请日：1997-12-23

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  ULRICH HEINRICH

IPC: G02B26/10 ,  G02B21/00

Abstract: The invention relates to an optical device for scanning a beam in two axes that are substantially perpendicular to each other, for use in particular in confocal laser scan microscopes, and aims to avoid serious image defects. The invention is characterized in that it has three mirrors (1, 2; 3) of which two mirrors (1, 2) are fixedly positioned at an angle to each other so that they rotate together around the y-axis and in so doing rotate the beam (4) around a pivot point located on the axis of rotation (x-axis) of the third mirror (3) which rotates by itself.

公开(公告)号：DE10016377A1

公开(公告)日：2001-10-18

申请号：DE10016377

申请日：2000-04-04

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  HOFFMANN JUERGEN ,  STORZ RAFAEL ,  ULRICH HEINRICH ,  BEWERSDORF JOERG ,  BIRK HOLGER

IPC: G02B5/30 ,  G02B6/287 ,  G02B21/00 ,  G02B21/06 ,  G02B27/28 ,  G02F1/29 ,  G02F1/33 ,  G02B27/10

Abstract: The present invention concerns an apparatus for combining light from at least two laser light sources, preferably in the context of confocal scanning microscopy, and in order to make laser light sources of low output power usable as light sources, in particular for confocal scanning microscopy, is characterized in that the light from the laser light sources has at least approximately the same wavelength; and that at least one beam combining unit that combines the light beams in at least largely lossless fashion is provided.

公开(公告)号：DE10004233A1

公开(公告)日：2001-08-16

申请号：DE10004233

申请日：2000-02-01

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  ULRICH HEINRICH

IPC: G02B21/00 ,  G02B21/16 ,  G02B21/18

公开(公告)号：DE19833025A1

公开(公告)日：2000-03-09

申请号：DE19833025

申请日：1998-07-23

Applicant: LEICA MICROSYSTEMS

Inventor： ULRICH HEINRICH ,  BORLINGHAUS ROLF

IPC: G02B6/26 ,  G02B6/34 ,  G02B21/00 ,  G02B21/06 ,  H01S3/00 ,  G02B6/42

Abstract: The invention relates to an optical arrangement for transmitting short laser pulses in optical fibers (2), especially for injection into a laser microscope, preferably for use in two-photon laser microscopy. The inventive arrangement comprises a short-pulse laser light source (1) and at least one optical fiber (2). An optical device (3) for temporally modifying the laser pulses is provided between the laser light source (1) and the optical fiber (2). The optical arrangement is provided for compensating an unintentional fiber dispersion and non-linear effects resulting from high energy densities, mainly self-phase modulation. The invention is characterized in that the optical device (3) is configured as a section which is, for the most part, comprised of a transparent, dispersive medium and which is located in the beam path (4) in front of the optical fiber (2) which serves the time extension of the laser pulses (pulse spreading).

公开(公告)号：DE19654210C2

公开(公告)日：1999-12-09

申请号：DE19654210

申请日：1996-12-24

Applicant: LEICA MICROSYSTEMS

Inventor： ENGELHARDT JOHANN ,  ULRICH HEINRICH

IPC: G02B26/10 ,  G02B21/00

Abstract: The invention relates to an optical device for scanning a beam in two axes that are substantially perpendicular to each other, for use in particular in confocal laser scan microscopes, and aims to avoid serious image defects. The invention is characterized in that it has three mirrors (1, 2; 3) of which two mirrors (1, 2) are fixedly positioned at an angle to each other so that they rotate together around the y-axis and in so doing rotate the beam (4) around a pivot point located on the axis of rotation (x-axis) of the third mirror (3) which rotates by itself.