公开(公告)号：WO2020033681A3

公开(公告)日：2020-02-13

申请号：PCT/US2019/045697

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： YANG, Jongtae ,  WANG, Xin ,  HU, Yi-Zhen ,  GEE, Kyle ,  CHEN, Aimei ,  BENSON, Scott ,  EVANS, Brian ,  RUKAVISHNIKOV, Aleksey

IPC: C09B69/00 ,  C09B11/14 ,  G01N33/58 ,  C12Q1/6869

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.

公开(公告)号：WO2020033681A2

公开(公告)日：2020-02-13

申请号：PCT/US2019/045697

申请日：2019-08-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： YANG, Jongtae ,  WANG, Xin ,  HU, Yi-Zhen ,  GEE, Kyle ,  CHEN, Aimei ,  BENSON, Scott ,  EVANS, Brian ,  RUKAVISHNIKOV, Aleksey

IPC: C09B69/00 ,  C09B11/14 ,  G01N33/58 ,  C12Q1/6869

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

公开(公告)号：WO2018045278A1

公开(公告)日：2018-03-08

申请号：PCT/US2017/049838

申请日：2017-09-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  PIERCE BIOTECHNOLOGY, INC.

Inventor： DESAI, Surbhi ,  NLEND, Marie ,  GEE, Kyle ,  BAKER, Matthew ,  AGGELER, Robert ,  SWEENEY, Scott ,  RUKAVISHNIKOV, Aleksey ,  HUANG, Shih-Jung

IPC: G01N33/58 ,  G01N33/533

Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.

Abstract translation: 本公开涉及荧光染料领域，并且具体地涉及用于增加荧光信号和减少荧光猝灭的组合物和方法。

公开(公告)号：WO2017100283A1

公开(公告)日：2017-06-15

申请号：PCT/US2016/065325

申请日：2016-12-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： HEIL, Korbinian ,  NOTKA, Frank ,  GEE, Kyle ,  DALLWIG, Jason ,  KUHN, Phillip ,  CORRY, Schuyler

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C12Q1/6834 ,  C12Q2563/107 ,  C12Q2563/149 ,  C12Q2565/537

Abstract: This disclosure relates to methods, compositions, and related apparatuses for detection and quantification of nucleic acid molecules associated with a solid surface. Methods can include detecting or quantifying at least one nucleic acid molecule associated with a surface by contacting the at least one nucleic acid molecule with a fluorophore, wherein the fluorophore emits increased fluorescence at a wavelength when in contact with nucleic acid molecule, and detecting or measuring fluorescence from the fluorophore at the wavelength. Compositions can comprise a fluorophore and a nucleic acid molecule, wherein the nucleic acid molecule is associated with a surface, and the fluorophore has the property of emitting increased fluorescence at a wavelength when in contact with a nucleic acid molecule.

Abstract translation: 本公开涉及用于检测和定量与固体表面相关的核酸分子的方法，组合物和相关装置。 方法可以包括通过使至少一种核酸分子与荧光团接触来检测或定量至少一种与表面相关的核酸分子，其中荧光团在与核酸分子接触时在波长处发射增加的荧光，并且检测或测量 荧光团在波长处的荧光。 组合物可以包含荧光团和核酸分子，其中核酸分子与表面结合，并且荧光团在与核酸分子接触时具有在波长处发射增加的荧光的性质。

公开(公告)号：WO2016090076A2

公开(公告)日：2016-06-09

申请号：PCT/US2015/063593

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： ZHOU, Wenjun ,  GEE, Kyle ,  TENNICO, Yolanda ,  SLADE, Peter ,  KANG, Hee Chol ,  KHAN, Shaheer ,  EVANS, Brian ,  STRAY, James

IPC: C07D261/00 ,  G01N33/58

CPC classification number: C07C309/47 ,  C07C309/51 ,  C07C2603/50 ,  C07D311/84 ,  G01N33/582 ,  G01N2400/10

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

Abstract translation: 本公开涉及含有肼基取代基和任选的一个或多个带负电荷的基团的染料化合物，例如磺酸盐，磷酸盐，膦酸盐和/或羧酸盐基团以及含有氨基氧基取代基的染料化合物。 该化合物可用于检测含醛和酮基的分析物，包括例如聚糖。

公开(公告)号：WO2014089421A1

公开(公告)日：2014-06-12

申请号：PCT/US2013/073548

申请日：2013-12-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DALLWIG, Jason ,  GEE, Kyle ,  AHNERT, Nancy ,  KANG, Hee Chol ,  YANG, Jongtae

IPC: C09B23/00 ,  C12Q1/68 ,  G01N33/52 ,  G01N33/58 ,  G01N33/68

CPC classification number: C12Q1/6825 ,  C09B23/00 ,  C09B23/04 ,  C09B23/06 ,  C09B23/083 ,  C09B23/086 ,  C12Q1/6816 ,  C12Q1/6837 ,  C12Q2525/207 ,  C12Q2563/107 ,  C12Q2563/173

Abstract: Substituted unsymmetrical cyanine dyes are provided herein, including kits, assays systems and methods for their use in the detection of RNA compounds ( e.g ., miRNA).

Abstract translation: 本文提供取代的不对称花青染料，包括用于检测RNA化合物（例如miRNA）的试剂盒，测定系统和方法。

公开(公告)号：WO2012112609A2

公开(公告)日：2012-08-23

申请号：PCT/US2012/025123

申请日：2012-02-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LAKSHMIPATHY, Uma ,  SINGH, Upinder ,  GRECIAN, Scott ,  QUINTANILLA, Rene ,  GEE, Kyle ,  RAO, Mahendra

Inventor： LAKSHMIPATHY, Uma ,  SINGH, Upinder ,  GRECIAN, Scott ,  QUINTANILLA, Rene ,  GEE, Kyle ,  RAO, Mahendra

IPC: G01N33/58 ,  G01N33/50

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Abstract translation: 本发明涉及用于染色活的干细胞的新型底物和方法。 该染色剂可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：EP3227264B1

公开(公告)日：2020-10-21

申请号：EP15828557.7

申请日：2015-12-03

Applicant: Life Technologies Corporation

Inventor： ZHOU, Wenjun ,  GEE, Kyle ,  TENNICO, Yolanda ,  SLADE, Peter ,  KANG, Hee Chol ,  KHAN, Shaheer ,  EVANS, Brian ,  STRAY, James

IPC: C07D261/00 ,  G01N33/58

公开(公告)号：EP2831179B1

公开(公告)日：2016-04-20

申请号：EP13709645.9

申请日：2013-02-26

Applicant: Life Technologies Corporation

Inventor： HU, Yi-Zhen ,  CHEN, Aimei ,  KANG, Hee Chol ,  GEE, Kyle ,  MANDAVILLI, Bhaskar

IPC: C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/533 ,  G01N33/58

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

公开(公告)号：EP2966061A1

公开(公告)日：2016-01-13

申请号：EP15178237.2

申请日：2012-03-01

Applicant: Life Technologies Corporation

Inventor： GEE, Kyle ,  SINGH, Upinder ,  GRECIAN, Scott ,  CLARKE, Scott

IPC: C07D215/02 ,  G01N33/533

CPC classification number: G01N33/582 ,  C07D213/53 ,  C07D213/68 ,  C07D213/74 ,  C07D213/78 ,  C07D213/79 ,  C07D213/81 ,  C07D215/12 ,  C07D235/14 ,  C07D401/04 ,  C07D401/14 ,  C07K14/575 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  G01N33/533

Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.

Abstract translation: 提供了低铜点击化学，1.3偶极环加成和用于修饰生物分子的Staudinger连接。 还提供了与低铜点击化学，1.3偶极环加成和Staudinger连接有关的组合物，方法和试剂盒。