公开(公告)号：KR1020140046826A

公开(公告)日：2014-04-21

申请号：KR1020120112976

申请日：2012-10-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 안일평 ,  김동헌 ,  배신철 ,  황덕주 ,  박상렬

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/6895 ,  C12Q1/686

Abstract: The present invention relate to a composition for detecting Cochliobolus miyabeanus and a method for detecting Cochliobolus miyabeanus using the same, and more specifically, provides a primer set for detecting Cochliobolus miyabeanus and a probe for quantitative detection of Cochliobolus miyabeanus. When using a primer set and a probe according to the present invention, it is possible to accurately and promptly detect Cochliobolus miyabeanus at high sensitivity and to quantify progress degree of Bipolaris sorokiniana caused by Cochliobolus miyabeanus thereby objectively quantifying, and accordingly it is expected to be established as a molecular biological monitoring system in agriculture.

Abstract translation: 本发明涉及一种用于检测曲霉杆菌的组合物和使用该组合物检测曲霉菌的方法，更具体地说，提供了用于检测曲霉菌的引物组和用于定量检测曲霉杆菌的探针。 当使用根据本发明的引物组和探针时，可以高灵敏度准确地并且迅速地检测枸杞子，并且可以量化由Co iana iana iana iana iana of of of ify ify ify ify ify ify ify ify be be be be be be be be be be be 作为农业分子生物学监测系统。

公开(公告)号：KR1020140046820A

公开(公告)日：2014-04-21

申请号：KR1020120112965

申请日：2012-10-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 안일평 ,  김동헌 ,  배신철 ,  황덕주 ,  박상렬

IPC: A01N57/20 ,  A01P1/00

Abstract: The present invention relates to a composition for controlling bacterial blight comprising Glufosinate ammonium, and a method for controlling bacterial blight by processing the same, which are expected to minimize the damage of agriculture by allowing the effective control of bacterial blight. The composition for controlling bacterial blight contains the Glufosinate ammonium or salt thereof as an effective component. The concentration of the Glufosinate ammonium is less than 5 ppm.

Abstract translation: 本发明涉及一种用于防治细菌性枯萎病的组合物，其中包括草铵膦铵，以及通过处理它们来控制细菌性枯萎病的方法，预期通过允许有效控制细菌性病害来最小化农业的损害。 用于防治细菌性枯萎病的组合物含有作为有效成分的草铵膦或其盐。 草铵膦的浓度小于5ppm。

公开(公告)号：KR101293654B1

公开(公告)日：2013-08-07

申请号：KR1020110046796

申请日：2011-05-18

Applicant: 대한민국(농촌진흥청장)

Inventor： 김범기 ,  변명옥 ,  김동헌 ,  윤인선 ,  김둘이 ,  한세연 ,  문석준 ,  신동진 ,  황현식

IPC: A01H5/00 ,  C12N15/82

Abstract: 본 발명은 재조합 발현 벡터를 포함하는 아그로박테리아와 공동 배양하기 전에 형질전환 대상 식물체의 배유를 제거하는 단계를 포함하는 것에 특징이 있는 아그로박테리아를 이용한 식물체 형질전환 방법에 관한 것으로써, 본 발명에 따르면 대량의 형질전환을 고속고효율로 안정적으로 수행할 수 있다.

公开(公告)号：KR101174410B1

公开(公告)日：2012-08-22

申请号：KR1020080110858

申请日：2008-11-10

Applicant: 대한민국(농촌진흥청장)

Inventor： 이기종 ,  권순종 ,  김동헌 ,  김명식 ,  이경렬 ,  박용환 ,  김정봉 ,  김경환 ,  이부영

IPC: A01H1/00 ,  A01G1/00

Abstract: 본발명은α-토코페롤고 함유형질전환콩 및그의제조방법에관한것이다. 보다상세하게는콩 종자내부에다량으로함유되어있는γ-토코페롤을들깨에서유래한γ-토코페롤메틸트랜스퍼라제(γ-tmt)에의하여비타민 E 활성이월등히높은α-토코페롤로전환하여콩 종자내에α-토코페롤함량이증가하도록아그로박테리움투메파시엔스를이용한콩의형질전환방법및 이방법에의하여생산된콩에관한것이다.

公开(公告)号：KR101153463B1

公开(公告)日：2012-06-27

申请号：KR1020090071553

申请日：2009-08-04

Applicant: 대한민국(농촌진흥청장)

Inventor： 김동헌 ,  이근표 ,  김명일 ,  권유진 ,  김용삼

IPC: C12N15/29 ,  C07K14/415 ,  C12N15/82 ,  A01H1/00

CPC classification number: C07K14/415 ,  C12N15/8294

Abstract: The present invention pertains to an auxin receptor protein involved in activation of proton pump in plasma membrane of a plant derived from rice, a gene encoding the protein, a recombinant vector comprising the gene, a host cell transformed with the recombinant vector, a method of improving traits of a plant by transforming the plant with the recombinant plant expression vector, a plant having improved traits by transformation with the recombinant plant expression vector and seeds of the plant, and a composition comprising the gene of the invention for improving traits of a plant.

公开(公告)号：KR1020040075385A

公开(公告)日：2004-08-30

申请号：KR1020030010830

申请日：2003-02-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 김경환 ,  황영수 ,  김동헌 ,  박용환 ,  박홍재 ,  김용환

IPC: A01H5/00

CPC classification number: C12N15/8274 ,  A01H4/005 ,  A01H4/008 ,  C12N5/0025 ,  C12N15/8205 ,  C12N15/8209 ,  C12N2500/34 ,  C12N2501/30

Abstract: PURPOSE: A method for producing transgenic perilla plants and herbicide-resistant perilla plants produced therefrom are provided, thereby easily inserting foreign useful genes into perilla plants, and reducing the production cost and environmental pollution. CONSTITUTION: The method for producing transgenic perilla plants comprises the steps of: (A) constructing an expression vector for transforming plants containing a foreign target gene and a selection maker; (B) introducing the expression vector into Agrobacterium to produce transformed Agrobacterium; (C) co-culturing the transformed Agrobacterium with a tissue section of perilla plants; (D) inducing shoots of the tissue section of perilla plants co-cultured in a selection-shoot inducing medium and subculturing the shoots of perilla plants in a root-inducing medium; and (F) transplanting the root-induced perilla plants in the field.

Abstract translation: 目的：提供一种生产转基因紫苏植物和由其生产的除草剂抗性紫苏植物的方法，从而容易地将外来的有用基因插入紫苏植物中，降低生产成本和环境污染。 构成：生产转基因紫苏植物的方法包括以下步骤：（A）构建用于转化含有外源靶基因的植物和选择标记物的表达载体; （B）将表达载体导入农杆菌以产生转化的土壤杆菌; （C）用紫苏植物的组织切片共转化转化的土壤杆菌; （D）诱导在选择性芽诱导培养基中共培养的紫苏植物的组织切片的芽，并在根诱导培养基中传代培养紫苏植物的芽; 和（F）在田间移植根诱导的紫苏植物。

公开(公告)号：KR1020040066628A

公开(公告)日：2004-07-27

申请号：KR1020030003749

申请日：2003-01-20

Applicant: 대한민국(농촌진흥청장)

Inventor： 김태호 ,  김영태 ,  김동헌 ,  변명옥 ,  박용환 ,  고승주

IPC: C12N15/29

CPC classification number: C07K14/415 ,  A61K35/74 ,  A61K38/168 ,  C12N15/70 ,  C12N15/8279 ,  C12Q1/6895

Abstract: PURPOSE: A gene which may be involved in signal transduction of disease resistance is provided, which can be useful for development of transgenic plants having disease resistance. CONSTITUTION: The gene which may be involved in signal transduction of disease resistance is DNA encoding a protein having 80% homology to the amino acid sequence set forth in SEQ ID NO: 1 or fragments thereof, wherein DNA has the nucleotide sequence set forth in SEQ ID NO: 2. An expression vector for inducing disease resistance of plants, pQE80L::(a,b,c) contains the gene encoding a protein having 80% homology to the amino acid sequence set forth in SEQ ID NO: 1. A microorganism transformed with the expression vector pQE80L::(a,b,c) is pQE80L:: a/E.coli CF1652(KACC 95010) or pQE80L::(a,b,c) is pQE80L:: b/E.coli CF1652(KACC 95011). A plant having disease resistance which is transformed with the expression vector pQE80L::(a,b,c) is provided.

Abstract translation: 目的：提供可能涉及抗病性信号转导的基因，可用于开发具有抗病性的转基因植物。 构成：可能涉及抗病性信号转导的基因是编码与SEQ ID NO：1所示的氨基酸序列或其片段具有80％同源性的蛋白质的DNA，其中DNA具有SEQ所示的核苷酸序列 编号：2.用于诱导植物抗病性的表达载体pQE80L：:( a，b，c）含有编码与SEQ ID NO：1所示的氨基酸序列具有80％同源性的蛋白质的基因。 表达载体pQE80L转化的微生物:( a，b，c）是pQE80L :: a /大肠杆菌CF1652（KACC 95010）或pQE80L：:( a，b，c）是pQE80L :: b / E.coli CF1652（KACC 95011）。 提供了用表达载体pQE80L转化的具有抗病性的植物:( :( a，b，c））。

公开(公告)号：KR1019980077719A

公开(公告)日：1998-11-16

申请号：KR1019970014958

申请日：1997-04-22

Applicant: 대한민국(농촌진흥청장)

Inventor： 김정봉 ,  김동헌 ,  임재현 ,  황영수

IPC: G01N31/22

Abstract: 본 발명은 DPPH(1,1-diphenyl-2-picrylhydrazyl)를 이용한 항산화물질의 분석방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 유리 라디칼(free radical)인 DPPH와 항산화물질을 반응시켜 DPPH의 보라색이 무색으로 변하는 색깔 변화를 관찰함으로써, 동·식물체 및 미생물 등에 존재하는 항산화물질을 신속·간단하면서도 경제적으로 검출 또는 정량하는 분석방법에 관한 것이다. 본 발명의 방법은 항산화물질의 탐색, 분리·정제에 이용될 수 있으며, 새로운 항산화물질의 개발 및 연구에도 이용될 수 있다.