公开(公告)号：KR1020150028121A

公开(公告)日：2015-03-13

申请号：KR1020130106818

申请日：2013-09-05

Applicant: 삼성전자주식회사

Inventor： 정순천 ,  박준성 ,  박진환 ,  윤지애 ,  박재찬 ,  조광명

IPC: C12N1/21 ,  C12N15/53 ,  C12N15/77 ,  C12P7/40

CPC classification number: C12P7/46 ,  C12N9/0008 ,  C12Y102/01002

Abstract: Provided are corynebacterium including an NAD+ dependent formate dehydrogenase gene, and a method for producing C4 dicarboxylic acid using the same. The production of reductive metabolites can increase under an anaerobic condition without adding formate, a substrate, by using the corynebacterium.

Abstract translation: 提供了包括NAD +依赖性甲酸脱氢酶基因的棒状杆菌，以及使用其制备C4二羧酸的方法。 还原代谢物的产生可以在厌氧条件下增加，而不需要通过使用棒状杆菌来添加甲酸盐，底物。

公开(公告)号：KR1020150025482A

公开(公告)日：2015-03-10

申请号：KR1020130103427

申请日：2013-08-29

Applicant: 삼성전자주식회사

Inventor： 이우용 ,  박준성 ,  이영민 ,  박재찬 ,  박진환

IPC: C12N1/21 ,  C12N15/53 ,  C12N15/70 ,  C12P7/16

CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12Y102/04001

Abstract: An aspect of the present invention relates to Pdh with an increased activity under an anaerobic condition, and a microorganism including the same. If the Pdh activity increases under the anaerobic condition, an acetyl-CoA metabolism process can be compelled. Accordingly, a compound can be produced by using metabolism products of a TCA cycle. Furthermore, the microorganism produced thereby can increase the production amount of 1,4-BDO under the anaerobic condition. The method can be applied to various microorganisms used in a fermentation process. The method contributes to the yield improvement of the fermentation process, thereby being usefully used in industry.

Abstract translation: 本发明的一个方面涉及在厌氧条件下具有增加的活性的Pdh和包含该Pdh的微生物。 如果Pdh活性在厌氧条件下增加，则乙酰辅酶A代谢过程可能被迫。 因此，可以通过使用TCA循环的代谢产物来制备化合物。 此外，由此生产的微生物可以在厌氧条件下增加1,4-BDO的产生量。 该方法可以应用于发酵过程中使用的各种微生物。 该方法有助于发酵过程的产量提高，从而在工业中有用。

公开(公告)号：KR1020140122135A

公开(公告)日：2014-10-17

申请号：KR1020130038846

申请日：2013-04-09

Applicant: 삼성전자주식회사

Inventor： 김태용 ,  이규상 ,  박재찬

IPC: C12N1/21 ,  C12N15/52 ,  C12N15/70 ,  C12P7/40

Abstract: 일 양상에 따른 미생물은 자연적으로 존재하지 않는 아디페이트 생합성을 위해 적어도 하나 이상의 외부 유전자가 도입되고, 추가적으로 적어도 하나의 유전자가 결실되었다. 이렇게 형질전환된 미생물은 종래의 아디페이트를 생합성하는 미생물에 비하여 현저하게 아디페이트를 생산할 수 있다. 이러한 형질전환된 미생물은 화학합성에 비하여 효율적으로 아디페이트를 생산할 수 있다.

Abstract translation: 已经诱导了根据本发明的一个方面的微生物至少一种外源基因，另外缺少至少一个基因以生物合成己二酸酯。 与现有的生物合成己二酸的微生物相比，转基因微生物可以产生显着更多的己二酸酯。 与化学合成相比，转基因微生物可以有效地生产己二酸酯。

公开(公告)号：KR1020140110613A

公开(公告)日：2014-09-17

申请号：KR1020130025247

申请日：2013-03-08

Applicant: 삼성전자주식회사

Inventor： 이소영 ,  윤소정 ,  김태용 ,  박재찬 ,  박진우 ,  이규상

IPC: G06F19/00 ,  G06F17/40

CPC classification number: G06F19/704

Abstract: A method for creating a toxicity to a microorganism prediction model, a method for predicting the toxicity of chemicals to microorganism using the toxicity prediction model created by the method, and a method for prioritizing biosynthetic pathways of a target substance using the toxicity prediction method are provided. The method can be used for evaluating the biosynthetic pathway by predicting the toxicity of intermediate metabolic substance when building a new biosynthetic pathway, prioritizing, or rebuilding the pathway.

Abstract translation: 本发明提供一种利用本方法生成的毒性预测模型来预测微生物预测模型的毒性的方法，使用该毒性预测方法对目标物质的生物合成途径进行优先排序的方法来预测化学物质对微生物的毒性的方法 。 该方法可用于通过在建立新的生物合成途径，优先化或重建途径时预测中间体代谢物质的毒性来评估生物合成途径。

公开(公告)号：KR1020140016654A

公开(公告)日：2014-02-10

申请号：KR1020120083513

申请日：2012-07-30

Applicant: 아주대학교산학협력단 ,  삼성전자주식회사

Inventor： 박진환 ,  이평천 ,  박재찬 ,  이영민 ,  이우용

IPC: C12N9/02 ,  C12N15/53 ,  C12P7/16 ,  C12N1/21

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12P7/24

Abstract: One embodiment confirms an ability to produce 1,4-butanediol as a result of being expressed within colon bacillus by introducing, from Clostridiumsaccharoperbutylacetonicum, butyraldehyde dehydrogenase (bld) and butanol dehydrogenase (bdh), which are enzymes activities of which have not been reported so far, for two reactions (4-hydroxybutyraldehyde producing reaction in 4-hydroxybutyryl-CoA and 1,4-butanediol producing reaction in 4-hydroxybutyraldehyde) at the end among 1,4-butanediol biosynthesis paths. In particular, the production concentration of 1,4-butanediol was enhanced by strengthening activities of the butyraldehyde dehydrogenase enzyme with a method of evolution in direction.

Abstract translation: 一个实施方案证实了通过从Clostridiumsaccharoperbutylacetonicum，丁醛脱氢酶（bld）和丁醇脱氢酶（bdh）引入的结肠杆菌中产生1,4-丁二醇的能力，其中酶活性尚未被报道 在1,4-丁二醇生物合成途径中的两个反应（4-羟基丁酰辅酶A中产生4-羟基丁醛产生反应和4-羟基丁醛中产生1,4-丁二醇的反应）。 特别地，1,4-丁二醇的生产浓度通过以方向发展的方法强化丁醛脱氢酶的活性来提高。

公开(公告)号：KR1020130124065A

公开(公告)日：2013-11-13

申请号：KR1020120047683

申请日：2012-05-04

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  구현민 ,  박성민 ,  권대혁 ,  김재영 ,  박재찬

IPC: C12N1/19 ,  C12N15/55 ,  C12P7/06 ,  C12N15/81

CPC classification number: Y02E50/17 ,  C12N15/815 ,  C12N9/2402 ,  C12P7/10 ,  C12P7/40 ,  C12P13/04 ,  C12P19/02 ,  C12P19/04

Abstract: Disclosed is modified microorganisms for simultaneous saccharification and fermentation, an expression vector for preparing the modified microorganisms, and a method for producing chemical materials using the modified microorganisms. According to one side, disclosed is modified Kluyveromyces marxianus which produces chemical materials by simultaneous saccharification and fermentation and contains a replication origin; a promoter; a gene encodes one or more cellulose decomposing enzymes selected among beta-glucosidase, endoglucanase, exoglucanase, and cellobiohydrolase; and a terminator.

Abstract translation: 公开了用于同时糖化和发酵的改性微生物，用于制备改性微生物的表达载体，以及使用改性微生物生产化学物质的方法。 一方面，公开了通过同时进行糖化和发酵生产化学物质的马克斯克鲁维酵母（Kluyveromyces marxianus），并含有复制起点; 启动子 一种基因编码一种或多种选自β-葡糖苷酶，内切葡聚糖酶，外切葡聚糖酶和纤维二糖水解酶的纤维素分解酶; 和终结者。

公开(公告)号：KR101273464B1

公开(公告)日：2013-06-14

申请号：KR1020060085670

申请日：2006-09-06

Applicant: 삼성전자주식회사

Inventor： 조경일 ,  박재찬 ,  허수봉 ,  한완택 ,  김민선

IPC: F25D11/00 ,  F25B49/00

CPC classification number: A01N1/02 ,  A01N1/0252 ,  A01N1/0294 ,  A23L3/36 ,  F25D23/12

Abstract: 본발명은냉장및 냉동제어시스템에관한것으로, 더욱상세하게는피보존물을품질의저하없이장기간보존할수 있고, 전기장의주파수범위(frequency range) 제어에의한과냉또는선도유지가되는냉장및 냉동제어시스템에관한것이다. 본발명은냉동및 냉장제어시스템에있어서, 대면하는양극과음극을포함하는전극모듈; 및상기양극과상기음극에전압을인가하여상기양극과상기음극사이에둘 이상의주파수범위(frequency range)를갖는전기장을형성하고, 상기인가되는전압의주파수범위를제어하는전기장인가모듈을포함하는것을특징으로하는냉장및 냉동제어시스템을제공하여, 피보존물을무동결상태에서미생물제어와산화억제가동시에이루어지도록한다.

Abstract translation: 一种制冷和冷冻控制系统及其方法，其长期存储没有质量劣化的保存对象，并且通过控制频率范围来控制过冷或保持新鲜度。 制冷和冷冻控制系统包括具有面向阳极的阳极和阴极的电极模块和向阳极和阴极施加电压的电场施加模块，其产生具有至少两个频率范围之间的电场 阳极和阴极，并控制施加电压的频率范围。

公开(公告)号：KR1020100093253A

公开(公告)日：2010-08-25

申请号：KR1020090012359

申请日：2009-02-16

Applicant: 삼성전자주식회사 ,  경기대학교 산학협력단

Inventor： 조화영 ,  김준석 ,  박재찬 ,  박성민 ,  김진우

IPC: C12P7/06 ,  C12P7/16 ,  C10G3/00 ,  C12R1/865

CPC classification number: C12P19/02 ,  C12M47/06 ,  C12M47/14 ,  C12P5/02 ,  C12P7/04 ,  C12P7/10 ,  C12P7/649 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/136 ,  Y02P30/20

Abstract: PURPOSE: A method and apparatus for pretreatment and saccharification of sea algae biomass is provided to prevent reduction of saccharide concentration regardless of time and space. CONSTITUTION: A method for pretreatment/saccharification of sea algae biomass comprises: a dehydrating sea algae bio mass by 10-70% of water content; a step of cutting the biomass in a certain size; and a step of treating the biomass with hydrolysis catalyst and/or hydrolase. An apparatus for pretreatment/saccharification of sea algae biomass comprises: a transfer pipe having a hollow as a transfer path of the sea algae biomass; an input port for inputting the biomass; a screw which provides the transfer driving force of the biomass by shaft rotation; a dehydrating device with an outlet for discharging the biomass; a cutting device which is connected to the one end of outlet side; and a saccharification device having a reactor for saccharification.

Abstract translation: 目的：提供海藻生物质的预处理和糖化的方法和装置，以防止糖浓度的降低，而不管时间和空间如何。 构成：海藻生物质预处理/糖化的方法包括：脱水海藻生物质含量为10-70％; 以一定尺寸切割生物质的步骤; 以及用水解催化剂和/或水解酶处理生物质的步骤。 一种用于海藻生物质的预处理/糖化的设备包括：具有作为海藻生物质的转移路径的中空的输送管; 用于输入生物量的输入端口; 螺杆，其通过轴旋转提供生物质的传递驱动力; 具有用于排出生物质的出口的脱水装置; 连接到出口侧的一端的切割装置; 和具有糖化反应器的糖化装置。

公开(公告)号：KR1020100047789A

公开(公告)日：2010-05-10

申请号：KR1020090060397

申请日：2009-07-02

Applicant: 삼성전자주식회사

Inventor： 김진우 ,  김준석 ,  박재찬 ,  조화영

IPC: D21C1/06 ,  D21C1/04

CPC classification number: C10L1/02 ,  A23V2002/00 ,  C08B37/0057 ,  C08H8/00 ,  C10G2300/1011 ,  C12M45/06 ,  C12P7/10 ,  C12P2201/00 ,  C13K13/002 ,  D21C1/04 ,  D21C1/06 ,  D21C5/00 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P30/20 ,  A23V2250/5108 ,  A23V2250/638

Abstract: PURPOSE: A sorting method of lignocellulose-based biomass and a sorting device thereof are provided to improve processing efficiency through a continuous process, and to prevent excessive decomposition of xylose by successively extracting lignin and xylose. CONSTITUTION: A sorting method of lignocellulose-based biomass includes the following steps: offering lignocellulose-based biomass(S1); extracting lignin from biomass by adding a first solvent dissolving the lignin(S2); extracting xylose by adding a second solvent dissolving hemicelluloses(S3); and extracting the remaining cellulose from the extracted biomass. A sorting device includes a reaction vessel dipping the biomass, a second solvent tank, a first solvent tank, a first reservoir storing the lignin, and a reservoir including a second reservoir.

Abstract translation: 目的：提供基于木质纤维素的生物质的分选方法及其分选装置，以通过连续方法提高加工效率，并通过连续提取木质素和木糖来防止木糖过度分解。 构成：基于木素纤维素的生物质的分选方法包括以下步骤：提供基于木质纤维素的生物质（S1）; 通过添加溶解木质素的第一溶剂（S2）从生物质中提取木质素; 通过加入溶解半纤维素的第二溶剂提取木糖（S3）; 并从提取的生物质中提取剩余的纤维素。 分选装置包括浸渍生物质的反应容器，第二溶剂罐，第一溶剂罐，储存木质素的第一储存器和包括第二储存器的储存器。

公开(公告)号：KR1020100047407A

公开(公告)日：2010-05-10

申请号：KR1020080106278

申请日：2008-10-29

Applicant: 삼성전자주식회사 ,  성균관대학교산학협력단

Inventor： 유병조 ,  박재찬 ,  구현민 ,  진용수 ,  이기성

IPC: C12N15/52 ,  C12N15/56 ,  C12N15/09

CPC classification number: C12N15/1034 ,  C07K14/395 ,  C12P7/04 ,  C12P7/06 ,  C12P7/16 ,  C12P7/28 ,  Y02E50/10 ,  Y02E50/17 ,  C12N15/52 ,  C12N9/24 ,  C12N15/09

Abstract: PURPOSE: An SNR84 gene enhancing usability of galactose metabolism is provided to enhance metabolic rate of galactose and enhance productivity of bioalcohol from carbon source. CONSTITUTION: An SNR84 gene enhances galactose metabolism rate. The gene is denoted by sequence number 1. A pRS424 recombinant vector contains the SNR84 gene of sequence number 1. A transformed recombinant microorganism is obtained using the recombinant vector. The recombinant microorganism overexpresses the SNR84 gene. The microorganism is yeast. The yeast is Saccharomyces sp., Pachysolen sp., Clavispora sp., Kluyveromyces sp., Debaryomyces sp., Schwanniomyces sp., Candida sp., Pichia sp., or Dekkera sp. The deposit number KCTC 11388 BP of the recombinant microorganism is CEN.PK2-1D/pRS424-SNR84.

Abstract translation: 目的：提供增强半乳糖代谢可用性的SNR84基因，以提高半乳糖的代谢率，并提高生物醇从碳源的生产力。 构成：SNR84基因增强半乳糖代谢率。 该基因由序列号1表示.PRS424重组载体含有序列号1的SNR84基因。使用重组载体获得转化的重组微生物。 重组微生物过表达SNR84基因。 微生物是酵母。 酵母是酵母属（Saccharomyces sp。），Pachysolen sp。，Clavispora sp。，Kluyveromyces sp。，Debaryomyces sp。，Schwanniomyces sp。，Candida sp。，Pichia sp。或Dekkera sp。 重组微生物的保藏号KCTC 11388 BP为CEN.PK2-1D / pRS424-SNR84。