中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

39 records (took 0.023 seconds)

    트랜스포존 시스템을 이용한 혼합배양 시 목적균주의 검출및 모니터링 방법
    31.
    发明公开
    트랜스포존 시스템을 이용한 혼합배양 시 목적균주의 검출및 모니터링 방법 无效
    使用TRANSPOSON系统的混合文化中目标菌株的检测和监测方法

    公开(公告)号：KR1020090022978A

    公开(公告)日：2009-03-04

    申请号：KR1020070095468

    申请日：2007-09-19

    Applicant: 충북대학교 산학협력단 ,  강원대학교산학협력단

    Inventor： 엄현주 ,  한남수 ,  김명동

    IPC: C12Q1/68 ,  C12Q1/04

    CPC classification number: C12Q1/04 ,  C12N15/09

    Abstract: A method for monitoring and detecting a target strain is provided to maintain segregational stability by injecting a gene of a selection marker into chromosome DNA of a host cell and selectively detect the target strain from the same strains when the same strains are mix-cultivated using a transposon system. A method for monitoring and detecting a target strain comprises the following steps of: introducing a gene which can work as a selection marker in a genome of the target strain using a transposon system; and detecting and monitoring the target strain using the selection marker. The transposon system consists of a DNA fragment and transposase.

    Abstract translation: 提供了用于监测和检测靶菌株的方法，以通过将选择标记的基因注射到宿主细胞的染色体DNA中来保持分离稳定性，并且当使用相同的菌株混合培养相同的菌株时，从相同菌株中选择性地检测靶菌株 转座子系统 用于监测和检测靶菌株的方法包括以下步骤：使用转座子系统引入可在靶菌株的基因组中作为选择标记的基因; 并使用选择标记物检测和监测目标菌株。 转座子系统由DNA片段和转座酶组成。

    제한효소 절단에 의한 클로닝에 기반을 둔 표적 유전자파쇄방법
    33.
    发明公开
    제한효소 절단에 의한 클로닝에 기반을 둔 표적 유전자파쇄방법 有权
    基于通过限制酶切割克隆的靶基因破坏方法

    公开(公告)号：KR1020060112809A

    公开(公告)日：2006-11-02

    申请号：KR1020050035445

    申请日：2005-04-28

    Applicant: 재단법인서울대학교산학협력재단

    Inventor： 이태희 ,  김명동 ,  오용주 ,  서진호

    IPC: C12N15/09

    Abstract: A method for disrupting a target gene based on cloning through restriction enzyme cutting is provided to show improved accuracy, convenience and increased transformation efficiency compared to conventional methods, thereby being useful for genetic engineering and bio-industries. In the method for disrupting a target gene by inserting a nucleotide sequence fragment(c) which includes a nucleotide sequence fragment(a) located at the upper portion of 5' terminal at an open leading frame starting point of the target frame, and a nucleotide sequence fragment(b) from an open leading frame starting point of a selective marker to a certain point of the inside of the open leading frame starting point of the selective marker in sequence from the 5' terminal side; and a nucleotide fragment(f) which includes a nucleotide sequence fragment(d) from the starting point formed to have an overlapped portion with the nucleotide sequence fragment(b) to an open leading frame ending point of the selective marker and a nucleotide sequence fragment(e) located at the lower portion of 3' terminal at the open leading frame ending point of the target gene in sequence from the 5' terminal side into a host, the nucleotide sequence fragment(c) is obtained from the nucleotide sequence fragment(a) inserted via a restriction enzyme site and a vector(A) including all gene sequence of the selective marker and the nucleotide sequence fragment(f) is obtained from the all gene sequence of the selective marker and a vector(B) including the nucleotide sequence fragment(e) inserted via the restriction enzyme site.

    Abstract translation: 提供了一种基于通过限制酶切割克隆来破坏靶基因的方法，以显示与常规方法相比提高的准确性，方便性和增加的转化效率，从而可用于遗传工程和生物工业。 在通过将位于5'末端的上部的核苷酸序列片段（a）的核苷酸序列片段（c）插入目标框架的开放的起始帧起始点的方法来破坏靶基因的方法， 序列片段（b）从选择性标记的开放引导帧起始点到选择性标记的开放引导帧起始点的内部的某个点从5'末端侧开始; 以及核苷酸片段（f），其包含起始点的核苷酸序列片段（d），其与核苷酸序列片段（b）的重叠部分与选择性标记的开放的前端结束点和核苷酸序列片段 （e）从5'末端侧依次位于靶基因的开放引导框架终点的3'末端的下位置，宿主核苷酸序列片段（c）由核苷酸序列片段（ a）通过限制酶位点插入，并且从选择性标记的全部基因序列和包含核苷酸的载体（B）获得包含选择性标记的所有基因序列和核苷酸序列片段（f）的载体（A） 通过限制酶位点插入的序列片段（e）。

    고령자용 전화기
    37.
    发明公开
    고령자용 전화기 有权
    一个老人的电话

    公开(公告)号：KR1020100024191A

    公开(公告)日：2010-03-05

    申请号：KR1020080082938

    申请日：2008-08-25

    Applicant: 강원대학교산학협력단

    Inventor： 김명동 ,  서동완 ,  박은희 ,  조제원

    IPC: H04M1/60 ,  H04M1/00

    CPC classification number: H04M1/60

    Abstract: PURPOSE: A telephone for an aged person is provided to alternatively output ring tone of frequency band suitable to auditory characteristic of each user, thereby easily confirming a call alarm. CONSTITUTION: A ring tone output unit(30) alternatively outputs ring tone of at least two kinds of frequency bands. A ring tone control unit(40) controls a ring tone output unit. The main control unit(70) applies a control signal to the ring tone control unit, based on an input signal through a transceiver unit(14). The ring tone of at least 2 kinds of frequency bands is a ring tone of low frequency band suitable to auditory characteristic of an aged person and high frequency band suitable to auditory characteristic of a general person.

    Abstract translation: 目的：提供老年人的电话，以交替输出适合每个用户的听觉特征的频段的铃声，从而容易地确认呼叫报警。 构成：铃声输出单元（30）交替地输出至少两种频带的铃声。 铃声控制单元（40）控制铃声输出单元。 主控制单元（70）基于通过收发器单元（14）的输入信号向铃声控制单元施加控制信号。 至少2种频带的铃声是适合于老年人的听觉特征的低频带和适合于一般人的听觉特征的高频带的铃音。

    레드와인이 첨가된 산천어의 비린내 제거용 조성물의제조방법 및 이를 이용한 산천어 스테이크 제조방법
    38.
    发明授权
    레드와인이 첨가된 산천어의 비린내 제거용 조성물의제조방법 및 이를 이용한 산천어 스테이크 제조방법 失效
    使用ONCORHYNCHUS MASOU THENEOF加入红葡萄酒和炖牛肉的ON ON US OF OF OF OF OF OF OF OF OF OF OF OF OF OF OF OF OF

    公开(公告)号：KR100936073B1

    公开(公告)日：2010-01-14

    申请号：KR1020080067719

    申请日：2008-07-11

    Applicant: 강원대학교산학협력단 ,  재단법인 나라

    Inventor： 최면 ,  김명동 ,  황하진 ,  임재천 ,  조성연 ,  김상희

    IPC: A23L1/39 ,  A23L1/325 ,  A23L1/221

    CPC classification number: A23L27/84 ,  A23L13/60 ,  A23L17/10 ,  A23L27/105 ,  A23L27/14

    Abstract: PURPOSE: A manufacturing method of composition for smell elimination of Oncorhynchus masou and a steak manufacturing method using the same method are provided to maintain flesh and taste of the Oncorhynchus masou while removing fishy smell of the Oncorhynchus masou. CONSTITUTION: A manufacturing method of composition for smell elimination of Oncorhynchus masou includes the following steps: manufacturing a compound by adding water 67.5-83.5 weight%, dried Polygonati Rhizoma 2.5-6.5 weight%, dried Schizandrae Fructus 0.8-1.8 weight%, dried Lycii Fructus 0.7-1.7 weight%, glycyrrhizae radix 2-4 weight%, alive lotus root 8-15 weight%, garlic 1.1-2.5 weight%, and ginger 0.7-1.7 weight%; heating the compound for 30 ~ 180 minutes; filtering and cooling the compounds in 3-10°C and adding red wind on the heated compound.

    Abstract translation: 目的：提供Oncorhynchus masou的消除臭味的组合物的制造方法和使用相同方法的牛排制造方法，以保持Oncorhynchus masou的肉和味道，同时除去Oncorhynchus masou的鱼腥味。 构成：Oncorhynchus masou的气味消除用组合物的制造方法，包括以下步骤：通过加入水67.5-83.5重量％，干燥的Polygonati Rhizoma 2.5-6.5重量％，干燥的Schizandrae Fructus 0.8-1.8重量％，干燥的Lycii 果实0.7-1.7重量％，甘草根2-4重量％，活藕8-15重量％，大蒜1.1-2.5重量％，姜0.7-1.7重量％; 加热化合物30〜180分钟; 在3-10℃过滤和冷却化合物，并在加热的化合物上加入红色风。

    유산균 검정방법 및 검정용 프라이머
    39.
    发明授权
    유산균 검정방법 및 검정용 프라이머 失效
    유산균검정방법및검정용프라이머

    公开(公告)号：KR100452082B1

    公开(公告)日：2004-10-08

    申请号：KR1020010065808

    申请日：2001-10-24

    Applicant: 재단법인서울대학교산학협력재단 ,  주식회사 바이오앤진 ,  주식회사 보락

    Inventor： 서진호 ,  고영호 ,  김명동 ,  정수련 ,  김상용

    IPC: C12Q1/68

    Abstract: PURPOSE: Provided are a method of quantitatively and qualitatively analyzing lactic acid bacteria and a PCR primer which is capable of quantitatively and qualitatively analyzing lactic acid bacteria present in fermented foods. The quantitative and qualitative analysis can be carried out by performing QC-PCR with a PlaF/PlaR primer and an MesF/MesR primer to determine the fermentation degree and aging degree of fermented foods, and the storage state and quality thereof. CONSTITUTION: A method for quantitatively analyzing lactic acid bacteria comprises the step of: adding a target DNA to a sample and mixing a competitor Cmes or Cpla and performing PCR with a specific primer set of Lactobacillus plantarum or a specific primer set of Leuconostoc mesenteroides; measuring PCR amplification of the competitor and target DNA; and calculating the concentration of the target DNA concentration from the PCR amplification rate and the concentration of the competitor.

    Abstract translation: 目的：提供定量和定性分析乳酸菌的方法和能够定量和定性分析发酵食品中存在的乳酸菌的PCR引物。 定量和定性分析可以通过使用PlaF / PlaR引物和MesF / MesR引物进行QC-PCR来确定发酵食品的发酵程度和老化程度，以及其储存状态和质量。 构成：定量分析乳酸菌的方法包括以下步骤：向样品中加入靶DNA并混合竞争者Cmes或Cpla，并用特定的植物乳杆菌（Lactobacillus plantarum）引物组或特定的Leuconostoc mesenteroide引物组进行PCR; 测量竞争者和靶DNA的PCR扩增; 并根据PCR扩增速率和竞争者的浓度计算目标DNA浓度的浓度。

Patent Agency Ranking