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公开(公告)号:JPH04117285A
公开(公告)日:1992-04-17
申请号:JP12320290
申请日:1990-05-15
Applicant: AGENCY IND SCIENCE TECHN , HITACHI CHEMICAL CO LTD
Inventor: BABA KENZO , IWAKURA MASAHIRO , OHASHI SHINICHI , TANAKA YOSHIO , IZUTSU HIROSHI , OBARA KAZUHIKO , TAKASUKA AKIKO
IPC: C12N15/09 , A61K38/00 , A61K38/44 , A61P37/08 , C07K1/12 , C07K1/22 , C07K7/06 , C07K14/00 , C07K19/00 , C12N1/21 , C12N9/02 , C12N15/12 , C12N15/53 , C12N15/62 , C12P21/02 , C12R1/19
Abstract: NEW MATERIAL:A recombinant plasmid containing a base sequence encoding dihydrofolate reductase-antiallergic peptide polymer fused protein (I). EXAMPLE:A recombinant plasmid wherein an antiallergic pentapeptide, a fused protein (I) of dimer contains an amino acid sequence shown by the formula (underline shows amino acid existing between dihydrofolate reductase and antiallergic pentapeptide). USE:Production of DSDGK. PREPARATION:A DNA encoding DSDGK polymer obtained by chemically synthesizing a single strand DNA and annealing both chains is inserted into a vector plasmid capable of manifesting DHFR.
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公开(公告)号:JPH03156293A
公开(公告)日:1991-07-04
申请号:JP29307689
申请日:1989-11-10
Applicant: AGENCY IND SCIENCE TECHN
Inventor: YAMAMOTO ITARU , TANAKA YOSHIO
IPC: F28D15/02
Abstract: PURPOSE:To prevent dryup of the heat transfer surface of an evaporator by engraving cutouts at both upper and lower ends of a cylindrical laminated wick, opening a radial through hole at a position suitably separated from both ends, and concentrically disposing a columnar article with the wick in the evaporator of the vapor passage. CONSTITUTION:Heat is applied externally to the evaporator E of a heat pipe having a wick 11, work fluid is evaporated, vapor is fed to a condenser C, and condensed by heat radiating. The fluid is heated to generate bubbles in an air gap 12 of the evaporator E at the time of starting to heat to rise in two phases flow in the air gap 12. As the bubble rises, a cold work fluid flows from a cutout 11a, a through hole 11c to the gap 12. A part of the rising fluid is discharged through a cutout 11b and a through hole 11d to a vapor passage 13, condensed and fed down along the wick 11. As input heat is increased, the temperature of the fluid in the evaporator becomes uniform to be evaporated and boiled. The wick 11 is so extended as to slightly increase the interval of the air gap 12, a condensed liquid is always circulated, and local dryout is avoided due to sealing fluid quantity reducing effect by a columnar article 14.
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公开(公告)号:JPH02254292A
公开(公告)日:1990-10-15
申请号:JP7444589
申请日:1989-03-27
Applicant: AGENCY IND SCIENCE TECHN
Inventor: YAMAMOTO ITARU , TANAKA YOSHIO , KIZAWA TAKAO , NAKAZAWA KATSUHIRO
Abstract: PURPOSE:To reduce a size of a heat storage tank container, improve a heat storage capability and a thermal efficiency and further improve a thermal transporting capability and an operating characteristic by a method wherein the heat storage tank is arranged at a connection part of two circuits of a flow passage for performing a process under a difference of density of working fluid and another flow passage utilizing evaporation and condensation of the working fluid. CONSTITUTION:Working fluid 3 enclosed at a part of a working fluid flow passage 1 is heated by a heater 4, the heat is moved to a heat storage tank under a natural circulation, the heat is released and stored in the heat storage tank 5. The working fluid 3 discharged heat at the heat storage tank 5 and having its temperature lowered descends in a down-pipe 1-4 due to a difference in density and returns to the heater 4. As a temperature of the heat storage tank 5 reaches a predetermined temperature, an inputting of heat from the heater 4 is stopped and the heat is carried to a cooling unit 6 arranged at a connecting pipe 2-3 between as ascending pipe 2-1 at an upper part of the working fluid flow passage 2 and a down-pipe 2-2. The heat is discharged out of the pipe. The working fluid 3 discharged a latent heat through the cooling unit 6 is condensed and returns to the heat storage tank 5 through the down-pipe 2-2. With such an arrangement, it is possible to improve a thermal efficiency as well as a heat storage capability, provide a small-sized device and improve an operating characteristic.
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公开(公告)号:JPH02100681A
公开(公告)日:1990-04-12
申请号:JP25226588
申请日:1988-10-06
Applicant: AGENCY IND SCIENCE TECHN
Inventor: IZUKURA MASAHIRO , SAKAI TSUKASA , TANAKA YOSHIO
IPC: C12N15/09 , C07K1/16 , C07K1/22 , C07K14/00 , C07K14/575 , C07K14/655 , C07K14/675 , C07K19/00 , C12N1/20 , C12N1/21 , C12N15/12 , C12N15/62 , C12P21/02 , C12R1/19
Abstract: PURPOSE:To obtain a recombinant plasmid pENDB 15 capable of producing beta-endorphin stably replicated in Escherichia coli and having strong analgesic activity and having DNA sequence of 4718 base pair. CONSTITUTION:The novel recombinant plasmid pENDB15 has size of 4718 base pair and provides trimethoprim resistance and ampicillin resistance to Escherichia coli which is a host. The above-mentioned plasmid is obtained by replacing sequence of 26 base pair containing sequence coding MEK between BamHI site of pMEK2 (patent application No.63-79680) and XhoI site with chemically synthesized DNA of 104 base pair containing sequence coding beta- endorphin. Escherichia coli transformed by the pENDB15 is deposited as FERM BP-2029 to Fermentation Research Institute.
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公开(公告)号:JPH0279978A
公开(公告)日:1990-03-20
申请号:JP23124888
申请日:1988-09-14
Applicant: AGENCY IND SCIENCE TECHN
Inventor: IWAKURA MASAHIRO , OHASHI SHINICHI , SAKAI TSUKASA , TANAKA YOSHIO
IPC: C12N15/09 , A61K38/22 , A61P23/00 , A61P25/04 , C07K1/16 , C07K1/22 , C07K14/00 , C07K14/575 , C07K14/655 , C07K14/675 , C07K19/00 , C12N1/20 , C12N1/21 , C12N15/16 , C12N15/62 , C12P21/02 , C12R1/19
Abstract: PURPOSE:To obtain a recombinant plasmid pENDD2 being a recombinant plasmid having 4682 pair of bases and capable of producing delta-endorphin of morphine like peptide having high analgesic activity when Escherichia coli is transformed by the above-mentioned plasmid. CONSTITUTION:Gene coding delta-endorphin expressed by the formula is synthesized and the synthesized gene is integrated into a plasmid pMEK2 to prepare a fused gene (pENDCD2) of delta-endorphin gene and dihydrofolic acid reducing enzyme (DHFR) gene, by which Escherichia coli is then transformed. DHFR-delta- endorphin fused protein is produced by culturing the transformed Escherichia coli and then the fused protein is digested by a proteolytic enzyme to separate delta-endorphin.
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公开(公告)号:JPH01252286A
公开(公告)日:1989-10-06
申请号:JP7967788
申请日:1988-03-31
Applicant: AGENCY IND SCIENCE TECHN
Inventor: IWAKURA MASAHIRO , OHASHI SHINICHI , SAKAI TSUKASA , TANAKA YOSHIO
IPC: C12N15/09 , C07K1/12 , C07K1/16 , C07K1/22 , C07K7/18 , C07K14/00 , C07K19/00 , C12N1/20 , C12N1/21 , C12N9/06 , C12P21/02 , C12R1/19
Abstract: PURPOSE:To obtain a large amount of dihydrofolic acid reductase-bradykinin fused protein, by manifesting a fused gene in which a bradykinin gene is included in a plasmid vector pTP 70-1 with a colon bacillus. CONSTITUTION:A newly recombined plasmid pBK 1-11, which is replicated in stable with a colon bacillus, imparting resistance to trimethoprim and resistance to ampicillin to a colon bacillus as a host, having a size of 4645 basic pairs and having a bonded structure of chemically synthesized DNA of 37 basic pairs containing a conformation coding bradykinin at BamHI site of pTP 70-1, is produced. A colon bacillus introduced of pBK 1-11 (FERMBP-1817) produces a large amount of dihydrofolic acid reductase-bradykinin fused protein.
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公开(公告)号:JPH01144995A
公开(公告)日:1989-06-07
申请号:JP30215587
申请日:1987-11-30
Applicant: AGENCY IND SCIENCE TECHN
Inventor: IWAKURA MASAHIRO , KOKUBU TOMOKUNI , OHASHI SHINICHI , SAKAI TSUKASA , TANAKA YOSHIO
IPC: C07K14/655 , C07K1/16 , C07K1/22 , C07K14/00 , C07K14/195 , C07K14/41 , C07K14/575 , C12N9/06 , C12N15/09 , C12P21/02 , C12R1/19
Abstract: PURPOSE:To enable the separation and purification of a dihydrofolic acid reductase-somatostatin fused protein produced by E.coli transformed with plasmid pTPGIF2, by purifying the protein using a specific chromatography. CONSTITUTION:Escherichia coli containing plasmid pTPGIF2 (FERM BP-1577) is cultured and the obtained bacterial cells are disintegrated and centrifuged. The cell-free extraction liquid of the supernatant is subjected to ion-exchange column, methotrexate-bonded affinity column chromatography and anion- exchange column chromatography using dihydrofolic acid reductase activity as a criterion. The objective dihydrofolic acid reductase-somatostatin fused protein shown in the figure can be produced by this process in purified state.
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公开(公告)号:JPH01144979A
公开(公告)日:1989-06-07
申请号:JP30215787
申请日:1987-11-30
Applicant: AGENCY IND SCIENCE TECHN
Inventor: IWAKURA MASAHIRO , FURUSAWA KIYOTAKA , SAKAI TSUKASA , TANAKA YOSHIO
Abstract: PURPOSE:To produce a substance containing a heterogenic product fused to a carboxy-terminal of dihydrofolic acid reductase(DHFR), in high efficiency, by modifying a plasmid pTP70-1 capable of easily utilizing a DHFR gene as a genetic marker. CONSTITUTION:A terminator region of rrnB gene (one of ribosomal RNA genes) known as an efficient terminator is introduced at the downstream side of a DHFR gene of plasmid pTP70-1 to obtain a plasmid pTP104-4 capable of efficiently producing a substance containing heterogenic product fused to the carboxy-terminal of DHFR. The plasmid has a size of 4,466 base pairs and can impart a host E.coli with trimethoprim resistance and ampicillin resistance.
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公开(公告)号:JPH01144978A
公开(公告)日:1989-06-07
申请号:JP30215687
申请日:1987-11-30
Applicant: AGENCY IND SCIENCE TECHN
Inventor: IWAKURA MASAHIRO , KOKUBU TOMOKUNI , FURUSAWA KIYOTAKA , OHASHI SHINICHI , SAKAI TSUKASA , TANAKA YOSHIO
Abstract: PURPOSE:To produce a peptide (GRF1-29) containing the factors from the 1st to the 29th of bovine growth hormone releasing factors, by using a specific recombinant plasmid pGRE2-15. CONSTITUTION:A recombinant plasmid is prepared by linking a GRF1-29 gene to the 3'-terminal of a dihydrofolic acid reductase (DHFR) gene using a plasmid vector pTP70-1 capable of fusing a heteropeptide to a carboxy-terminal or DHFR of E.coli. The plasmid is introduced into E.coli and expressed to enable the stable production and accumulation of a fused protein containing GRF1-29 bonded to the carboxy-terminal of DHFR in E.coli.
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公开(公告)号:JPH01104024A
公开(公告)日:1989-04-21
申请号:JP26199087
申请日:1987-10-16
Applicant: AGENCY IND SCIENCE TECHN
Inventor: TANAKA YOSHIO , SAKURABA EIGO
IPC: C07B41/02 , C07B53/00 , C07C29/136 , C07C29/143 , C07C31/02 , C07C31/125 , C07C33/18 , C07C33/20 , C07C33/30 , C07C33/46 , C07C69/732
Abstract: PURPOSE:To obtain the title secondary alcohol of high optical purity by clathrating a carbonyl compound with cyclodextrin, dispersing the clathrate in a solution of reducing agent, then recovering the reduced product from the clathrate. CONSTITUTION:A carbonyl compound of the formula: R COR (R , R are organic residue which do not inhibit the reduction of the carbonyl group, and may form a ring) is clathrated with cyclodextrin (which may be any isomers of alpha, beta, or gamma). The clathrate directly or after dried, is combined with a reducing agent which has been added to a supersaturated inorganic salt aqueous solution or dispersed in an organic solvent under conditions shielded from the light and kept at -20-50 deg.C for 1-100hr to effect the reduction reaction. After removal of unreacting reducing agent, the reaction mixture is extracted with diethyl ether or the like to give the subject compound. The compound is used as a synthetic intermediate of physiologically active substances.
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