公开(公告)号：DE102004035052A1

公开(公告)日：2006-02-16

申请号：DE102004035052

申请日：2004-07-20

Applicant: BASF AG

Inventor： SAUER UWE ,  MAMPEL JOERG ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  ZELDER OSKAR ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA

IPC: C12N1/00

Abstract: The present invention relates to microorganisms and methods for producing at least one sulfur-containing compound.

公开(公告)号：AR047151A1

公开(公告)日：2006-01-11

申请号：ARP040104736

申请日：2004-12-17

Applicant: BASF AG

Inventor： KROEGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN

IPC: F01D15/10 ,  F02C3/113 ,  F02C7/268 ,  C12N15/31 ,  C12N15/67 ,  C12N15/63 ,  C12N15/77 ,  C12N1/21 ,  C12P13/08 ,  C12P13/12 ,  C12R1/15 ,  C12R1/13

Abstract: Se refiere al uso de secuencias de ácidos nucleicos para la regulacion de la transcripcion y la expresion de genes, a los nuevos promotores y unidades de expresion propiamente dichos, a procedimientos para la modificacion o activacion del grado de transcripcion y/o grado de expresion de genes, a casetes de expresion que contienen las unidades de expresion, a microorganismos modificados genéticamente con grado de transcripcion y/o grado de expresion modificado o activado, así como también a procedimientos para la preparacion de productos biosintéticos por medio del cultivo de los microorganismos genéticamente modificados. Reivindicacion 1: Uso de un ácido nucleico con actividad promotora, que contiene: A) la secuencia de ácidos nucleicos SEQ. ID. NO. 1 o B) una secuencia derivada de esta secuencia por sustitucion, insercion o delecion de nucleotidos, la que presenta una identidad de por lo menos 90 % a nivel de ácidos nucleicos con la secuencia SEQ. ID. NO. 1, o C) una secuencia de ácidos nucleicos, que hibrida con la secuencia de ácidos nucleicos SEQ. ID. NO. 1 bajo condiciones severas o D) fragmentos funcionalmente equivalentes de las secuencias de A), B) o C), para la transcripcion de genes.

公开(公告)号：AR047057A1

公开(公告)日：2006-01-04

申请号：ARP040104740

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: A61K38/15 ,  G01N33/574 ,  C12N15/53 ,  C12N1/21 ,  C12P13/08 ,  C12R1/15

Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, lactato deshidrogenasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de lactato deshidrogenasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono hacia oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado

公开(公告)号：BR0215958A

公开(公告)日：2005-09-13

申请号：BR0215958

申请日：2002-11-29

Applicant: BASF AG

Inventor： POMPEJUS MARKUS ,  GER BURKHARD KR ,  DER HARTWIG SCH ,  ZELDER OSKAR ,  HABERHAUER GREGOR ,  HAEFNER STEFAN ,  KLOPPROGGE CORINNA

IPC: C07K14/34 ,  C12N15/31 ,  C12N15/77 ,  C12P13/12 ,  C12R1/13 ,  C12R1/15

公开(公告)号：BR0313760A

公开(公告)日：2005-07-12

申请号：BR0313760

申请日：2003-08-26

Applicant: BASF AG

Inventor： GER BURKHARD KR ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  DER HARTWIG SCHR ,  HOFNER STEFAN

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12 ,  C12R1/15

Abstract: Fermentative production of at least one sulfur-containing fine chemical (I) by fermenting a (I)-producing coryneform bacterium (A), concentrating (I) in medium or cells, then isolating it. (A) expresses at least one heterologous nucleotide sequence (II) that encodes a protein (III) with methionine synthase (metF) activity. An Independent claim is also included for preparation of a feed additive (B) that contains L-methionine by culturing/fermenting an L-Met producing microorganisms, removing water from the fermentation broth and optionally also up to 100 % of the biomass, then drying the treated broth to recover (B) in powdered or granular form.

公开(公告)号：AU2004299729A1

公开(公告)日：2005-06-30

申请号：AU2004299729

申请日：2004-12-17

Applicant: BASF AG

Inventor： KIEFER PATRICK ,  KLOPPROGGE CORINNA ,  WITTMANN CHRISTOPH ,  HAFNER STEFAN ,  HEINZLE ELMAR ,  ZELDER OSKAR ,  KROGER BURKHARD ,  SCHRODER HARTWIG

IPC: C12P13/08 ,  C12N9/16

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2004299728A1

公开(公告)日：2005-06-30

申请号：AU2004299728

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  HAFNER STEFAN ,  HEINZLE ELMAR ,  SCHRODER HARTWIG ,  KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  KIEFER PATRICK ,  WITTMANN CHRISTOPH

IPC: C12N9/04 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：AU2004298546A1

公开(公告)日：2005-06-30

申请号：AU2004298546

申请日：2004-12-15

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  KROGER BURKHARD ,  ZELDER OSKAR

IPC: C12P13/08 ,  C07K14/34 ,  C12N20060101 ,  C12N1/00 ,  C12N15/63 ,  C12N15/67 ,  C12N15/77 ,  C12P1/00 ,  C12P13/12

Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).

公开(公告)号：BR0213775A

公开(公告)日：2004-11-09

申请号：BR0213775

申请日：2002-10-31

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHROEDER HARTWIG ,  KROEGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C07H21/04 ,  C07K14/34 ,  C12N1/21 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12N15/31 ,  C12P13/08 ,  C12R1/15

Abstract: The invention relates to novel nucleic acid molecules, to the use thereof for constructing genetically improved microorganisms and to methods for preparing fine chemicals, in particular amino acids, with the aid of said genetically improved microorganisms.

公开(公告)号：BR0213779A

公开(公告)日：2004-10-13

申请号：BR0213779

申请日：2002-10-31

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  DER HARTWIG SCHR ,  GER BURKHARD KR ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N15/11 ,  C07K14/34 ,  C12N1/21 ,  C12N15/63 ,  C12P13/08 ,  C12N15/10 ,  C12R1/15 ,  C12R1/13

Abstract: Isolated nucleic acid (I) that encodes particular protein sequences (II) that have, at specific positions, an amino acid alteration. Independent claims are also included for: (1) Vector that contains at least one (I); (2) Host cell transfected with at least one vector of (1); and (3) Method for preparing fine chemicals by culturing cells of (2).