公开(公告)号：KR1020130092168A

公开(公告)日：2013-08-20

申请号：KR1020120013729

申请日：2012-02-10

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  이나훔

IPC: C12N15/53 ,  C12N15/63 ,  C12P7/22

Abstract: PURPOSE: Catechol type structural material of high value-added that produced by the catecholtype construction material manufacturing method is provided to be used as a bioactive substance for whitening agent, antioxidant, anticancer material and antiviral substance. CONSTITUTION: DNA having the DNA sequence of the sequence number 2 is provided to be used as a DNA coding the tyrosinase help protein. As a DNA coding the tyrosinase help protein, it provides DNA having the DNA sequence of sequence number 2 and the homogeny over 78%. As a DNA coding the tyrosinase help protein, it provides DNA having the DNA sequence of sequence number 4. As a DNA coding the tyrosinase help protein, it provides DNA having the DNA sequence of sequence number 4 and the homogeny over 78%. Tyrosinase help protein is developed by the DNA. A manufacturing method of catechol type structural material used the host cell converted into vector which has the DNA and the DNA coding tyrosinase as biocatalizer and converted the monophenol type structural material.

Abstract translation: 目的：通过儿茶酚型建筑材料制造方法生产的高附加值的儿茶酚型结构材料被用作增白剂，抗氧化剂，抗癌材料和抗病毒物质的生物活性物质。 构成：提供具有序列号2的DNA序列的DNA作为编码酪氨酸酶帮助蛋白质的DNA。 作为编码酪氨酸酶帮助蛋白质的DNA，它提供具有序列号2的DNA序列和78％以上的均质的DNA。 作为编码酪氨酸酶帮助蛋白质的DNA，它提供具有序列号4的DNA序列的DNA。作为编码酪氨酸酶帮助蛋白质的DNA，其提供具有序列号4的DNA序列和超过78％的均质的DNA。 酪氨酸酶帮助蛋白质由DNA开发。 使用转化为具有DNA的载体的宿主细胞和编码酪氨酸酶的DNA作为生物催化剂并转化单酚型结构材料的儿茶酚型结构材料的制造方法。

公开(公告)号：KR100968080B1

公开(公告)日：2010-07-08

申请号：KR1020090071732

申请日：2009-08-04

Applicant: 서울대학교산학협력단

Inventor： 김평일 ,  김병기 ,  주황수 ,  성창민 ,  김은미

IPC: C12N1/20 ,  C07K14/00 ,  C12P21/02 ,  C12R1/44

Abstract: PURPOSE: A Staphylococcus hominis MBBL 2-9 strain is provided to ensure antibacterial activity to bacteria and to prevent gram positive/antibiotics resistant pathogen. CONSTITUTION: An antibacterial peptide hominicin which is isolated from Staphylococcus hominis MBBL 2-9 has an amino acid of sequence number 1. The antibacterial peptide is themal resistant, acid resistant, basic resistant, and non-cytotoxicity. The antibacterial peptide contains DmIle(N1, N1-dimethyl isoleucine), Dhb(dehydrobutyrine), and Dmp(N2, N2-dimethyl-1,2-propanediamine). A medicinal composition for preventing gram positive pathogen or antibiotics resistant pathogen contains the antibacterial peptide as an active ingredient.

Abstract translation: 目的：提供人类葡萄球菌MBBL 2-9菌株，以确保对细菌的抗菌活性，并防止革兰氏阳性/抗生素抗性病原体。 构成：从人类金黄色葡萄球菌MBBL 2-9中分离得到的抗菌肽人参皂苷具有序列号1的氨基酸。抗菌肽具有抗药性，耐酸碱性，无细胞毒性。 抗菌肽含有DmIle（N1，N1-二甲基异亮氨酸），Dhb（脱氢丁内酯）和Dmp（N 2，N 2 - 二甲基-1,2-丙二胺）。 用于预防革兰氏阳性病原体或抗生素抗性病原体的药物组合物含有抗菌肽作为活性成分。

公开(公告)号：KR102251360B1

公开(公告)日：2021-05-13

申请号：KR1020180162079

申请日：2018-12-14

Applicant: 서울대학교산학협력단

Inventor： 이기원 ,  정수미 ,  양희 ,  김병기 ,  윤정한 ,  김종은 ,  김종훈

IPC: A23L33/10 ,  A61K31/352 ,  A61K36/48 ,  A61P1/12

Abstract: 본발명은기존의지사제와비교하여월등한항설사능을발휘하는것을특징으로하는오로볼을함유하는설사개선용식품조성물및 예방또는치료용약학조성물에관한것으로, 우수한설사멈춤효과를발휘하는데, 합성약물등에비해소재접근성이높고, 체내부작용이적어안정성확보가가능한장점이있다.

公开(公告)号：KR101718681B1

公开(公告)日：2017-03-22

申请号：KR1020140076720

申请日：2014-06-23

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  최윤희

IPC: C12N9/10 ,  C12N15/54 ,  C12N15/09 ,  C12P19/18 ,  G01N31/22

CPC classification number: C12N9/1051 ,  C12P19/00 ,  C12P19/18 ,  C12Q1/48 ,  C12Y204/01065 ,  G01N2333/91102

Abstract: 본발명은가용성단백질발현량및 활성이증대된α-1,3 푸코실전달효소변이체, α-1,3 푸코실전달효소변이체를코딩하는 DNA, α-1,3 푸코실전달효소변이체를코딩하는 DNA를포함하는재조합 DNA 벡터, 상기재조합 DNA 벡터로형질전환된숙주세포및 숙주세포의추출물, 3-푸코실올리고당의제조방법, 푸코실전달효소변이체제조방법및 푸코실전달효소의변이체탐색방법을제공하는것으로서, 본발명의α-1,3 푸코실전달효소변이체는가용성단백질의발현량및 효소활성이획기적으로향상된효과가있다.

公开(公告)号：KR101648342B1

公开(公告)日：2016-08-16

申请号：KR1020140076718

申请日：2014-06-23

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  최윤희

IPC: C12N15/54 ,  C12N15/09 ,  C12N9/12 ,  C12P19/18

CPC classification number: C12N9/12 ,  C12N15/09 ,  C12P19/18

Abstract: 본발명은헬리코박터파일로리 26695() 유래의α-1,2 푸코실전달효소 (α-1,2 fucosyltransferase)의난발현성재조합단백질에대한가용성단백질발현을증가시키는방법과그로인해생성된α-1,2 푸코실전달효소를코딩하는유전자, 상기유전자의발현방법, 단백질및 이효소를이용한α-1,2 푸코실올리고당의합성에관한것이다.

公开(公告)号：KR101574952B1

公开(公告)日：2015-12-07

申请号：KR1020130039938

申请日：2013-04-11

Applicant: 서울대학교산학협력단 ,  주식회사 진켐

Inventor： 김병기 ,  최윤희 ,  김종훈 ,  김대희 ,  우진석

IPC: C12Q1/48 ,  C12N15/52 ,  C12P19/04

Abstract: 본발명은시알산전이효소변이체의탐색방법과변이체의특성, 상기시알산전이효소변이체효소를암호화하는유전자및 이들을이용한시알릴올리고당의합성에관한것이다. 본발명은단백질공학적변이를통해개량된파스테우렐라유래의α-2,3 시알산전이효소와포토박테리움유래의α-2,6 시알산전이효소를제공한다. 또한본 발명의시알산전이효소변이체는 3’- 및 6’-시알릴올리고당생산반응에서부반응감소, 생산성증대및 이로인한생산비용절감을제공한다.

公开(公告)号：KR1020150081185A

公开(公告)日：2015-07-13

申请号：KR1020140000906

申请日：2014-01-03

Applicant: 서울대학교산학협력단

Inventor： 김병기 ,  배진형 ,  이평강 ,  정은옥

IPC: C12N1/21 ,  C12N15/70

Abstract: 본발명은대장균 ()를이용한긴사슬지방산(long-chain fatty acid) 생변환을향상시키는것이다. 즉, 세포내에서긴사슬지방산의안정성을올리고세포외의기질은세포내로더 신속하게흡수되도록만듦으로써생변환수율을증가시키고생산성또한향상시킨다.

Abstract translation: 本发明的目的是通过使用大肠杆菌细菌改善长链脂肪酸生物转化。 也就是说，通过改善长链脂肪酸的稳定性和通过使细胞外基质更快地吸收到细胞中，生物转化产率提高并且提高生产力。

公开(公告)号：KR1020100051758A

公开(公告)日：2010-05-18

申请号：KR1020080110718

申请日：2008-11-08

Applicant: 서울대학교산학협력단

Inventor： 이윤식 ,  김병기 ,  신동식 ,  김윤곤 ,  김은미 ,  김미라 ,  김재희

IPC: C12Q1/48 ,  G01N33/573

CPC classification number: C12Q1/485 ,  G01N2333/9121

Abstract: PURPOSE: A method for identifying protein kinase substrate specificity using a peptide library is provided to rapidly determining the substrate specificity of a protein phosphatase, and to examine the role of the protein phosphatase. CONSTITUTION: A method for identifying protein kinase substrate specificity comprises the following steps: forming a peptide library with a constant amino acid sequence on the surface of a solid support resin; forming a hybrid with randomized peptide libraries by repeating the previous step; reacting the peptide libraries with a phosphatase for forming the peptide library with phosphorylated threonine or serine; reacting an antibody combined with a biotin with the phosphorylated peptide library; reacting the obtained product with neutravidin or streptavidin including phosphatase or peroxidase; detecting the change of properties by the reaction of the phosphatase or the peroxidase for selecting the solid support resin with transformed peptide libraries; and an analyzing the amino acid sequence of the peptide after separating the peptide from the resin.

Abstract translation: 目的：提供使用肽文库鉴定蛋白激酶底物特异性的方法，以快速测定蛋白磷酸酶的底物特异性，并检查蛋白磷酸酶的作用。 构成：鉴定蛋白激酶底物特异性的方法包括以下步骤：在固体支持树脂的表面上形成具有恒定氨基酸序列的肽文库; 通过重复前述步骤与随机肽文库形成杂交体; 使肽文库与磷酸酶反应，用磷酸化苏氨酸或丝氨酸形成肽文库; 将与生物素结合的抗体与磷酸化肽文库反应; 使所得产物与中性链亲和素或链霉亲和素（包括磷酸酶或过氧化物酶）反应; 通过磷酸酶或过氧化物酶的反应检测特性的变化，用于用转化的肽文库选择固体支持树脂; 并分析肽与树脂分离后的氨基酸序列。