公开(公告)号：KR1020010076900A

公开(公告)日：2001-08-17

申请号：KR1020000004319

申请日：2000-01-28

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  권미윤

IPC: C07K7/00

CPC classification number: C07K7/08 ,  A61K38/10

Abstract: PURPOSE: The peptides being capable of specifically binding with sLeX is provided, which can be effectively used for inhibiting the infection with bacteria and developing vaccine, or as therapeutic agents. CONSTITUTION: The peptides being capable of specifically binding with sLeX represented by a sequence selected from SEQ ID NO 1 to SEQ ID NO 5. Wherein, the sLeX is a tetrasaccharide and a critical substance in signal transfer between the cells or the recognition of pathogen; the peptide having the sequence of SEQ ID NO 1 contains multiple repeated amino acid sequence of SHWDQPRPGLKP; and the peptide having the sequence of SEQ ID NO 2 contains multiple repeated amino acid sequence of AHWPRYSSPATA.

Abstract translation: 目的：提供能够与sLeX特异性结合的肽，其可有效用于抑制细菌感染和开发疫苗，或作为治疗剂。 构成：能够与由选自SEQ ID NO 1至SEQ ID NO 5的序列表示的sLeX特异性结合的肽。其中，sLeX是细胞之间的信号转移或病原体识别中的四糖和关键物质; 具有SEQ ID NO 1的序列的肽含有多重重复的SHWDQPRPGLKP的氨基酸序列; 并且具有SEQ ID NO 2的序列的肽含有AHWPRYSSPATA的多个重复的氨基酸序列。

公开(公告)号：KR1020010001607A

公开(公告)日：2001-01-05

申请号：KR1019990020954

申请日：1999-06-07

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  박호군 ,  정선주 ,  엄태연

IPC: C07H21/02

Abstract: PURPOSE: Provided is an RNA sequence, which binds to siallyl Lewis (sLe X), of a cancer cell with high specificity and affinity. Also, a method for detecting sLe X using the RNA sequence is provided. The RNA is excellent in binding strength or molecular specificity, therefore, it is useful in detecting the cancer cell and in developing a therapeutic agent thereof. CONSTITUTION: The RNA is selected from the group consisting of SEQ ID NO : 1 - SEQ ID NO : 19 and has the binding specificity and the affinity to sLe X which is a cell surface marker related to an inflammation and a metastasis. The RNA sequence is selected by the steps of preparing the RNA library, fixing the sLe X with CNBr-activating Sepharose 4B and carrying out a systematic evolution of ligand by exponential enrichment (SELEX) method. The detection method of the sLe X comprises the steps of : reacting the sLe X with the RNA sequence selected from SEQ ID NO : 1 - SEQ ID NO : 19; and assaying the RNA bound specifically the sLe X to detect the sLe X.

Abstract translation: 目的：提供具有高特异性和亲和力的癌细胞的与siall Lewis（sLe X）结合的RNA序列。 另外，提供了使用RNA序列检测sLe X的方法。 RNA结合强度或分子特异性优异，因此可用于检测癌细胞和开发其治疗剂。 构成：RNA选自SEQ ID NO：1 - SEQ ID NO：19，并且具有与作为与炎症和转移相关的细胞表面标志物的sLe X的结合特异性和亲和力。 通过制备RNA文库，用CNBr活化的Sepharose 4B固定sLe X并通过指数富集（SELEX）方法进行配体的系统进化来选择RNA序列。 sLe X的检测方法包括以下步骤：使sLe X与选自SEQ ID NO：1 - SEQ ID NO：19的RNA序列反应; 并测定特异性结合sLe X以检测sLe X的RNA。

公开(公告)号：KR1020010001605A

公开(公告)日：2001-01-05

申请号：KR1019990020951

申请日：1999-06-07

Applicant: 한국과학기술연구원

Inventor： 유재훈 ,  박호군 ,  천성민 ,  정선주

IPC: C07H21/02

Abstract: PURPOSE: Provided is an RNA sequence which has a binding specificity to cholesterol or derivatives thereof and which has an activity of a cholesterol esterase. Also, provided is a detection method of a lipophilic substance, especially LDL resulting in arteriosclerosis and a hypertension using the RNA sequence. The sequence is useful in an inhibitor blocking a bioreaction caused by endocrine disruptors and is used as an artificial catalyst without any side effect. CONSTITUTION: The RNA sequence is selected from the group consisting of SEQ ID NO : 1 - SEQ ID NO : 11 and has the activity of a cholesterol esterase. The RNA sequence is prepared by a 131 bp DNA sequence comprising 70 nucleotides of random sequence as shown in Fig. 1, wherein T7 RNA polymerase of 5'-primer promotes an in vitro transcription and the site of a restriction enzyme is useful in identifying that the inserts have been incorporated into the sequence. The detection method of the lipophilic substance comprises the steps of : reacting the lipophilic substance with the RNA sequence selected from SEQ ID NO : 1 - SEQ ID NO : 11; and assaying the RNA bonded specifically with the lipophilic substance to detect the lipophilic substance.

Abstract translation: 目的：提供对胆固醇或其衍生物具有结合特异性并具有胆固醇酯酶活性的RNA序列。 此外，提供亲脂性物质的检测方法，特别是导致动脉硬化的LDL和使用RNA序列的高血压。 该序列可用于阻断由内分泌干扰物引起的生物反应的抑制剂，并用作人造催化剂而没有任何副作用。 构成：RNA序列选自SEQ ID NO：1〜SEQ ID NO：11，具有胆固醇酯酶的活性。 RNA序列由包含70个随机序列核苷酸的131bp DNA序列制备，如图1所示。 1，其中5'-引物的T7RNA聚合酶促进体外转录，并且限制酶的位点可用于鉴定插入片段已经并入序列。 亲脂性物质的检测方法包括以下步骤：使亲脂性物质与选自SEQ ID NO：1〜SEQ ID NO：11的RNA序列反应; 并测定与亲脂性物质特异性结合的RNA以检测亲脂性物质。