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公开(公告)号：KR1019910007847B1
公开(公告)日：1991-10-02
申请号：KR1019890008030
申请日：1989-06-10
Applicant: 한국과학기술원
IPC: C12M1/40
Abstract: A process for preparing sponge-like macroporous gelatin microcarrier (I) comprises: (a) mixing gelatin soln. with forming agent, e.g., NaHCO3 or CaCO3 (The particle size is 20-30 μm or less), and adding the mixed soln of chloroform (II) and toluene (III) contg. 1-4% surfactant. (mixing ration of (II):(III) is 3:7); (b) agitating the mixt. at 300-1000rpm to form emulsion; (c) filtering it with 100 μm sieve and washing with D.W.; (d) cross-linking with glutaraldehyde, and drying to obtain the final product. The diameter of (I) is 150-500 μm and pore size of (I) is 20-50 μm.
Abstract translation: 制备海绵状大孔明胶微载体（I）的方法包括：（a）混合明胶溶胶。 与成型剂，例如NaHCO 3或CaCO 3（粒径为20-30μm以下），并加入氯仿（II）和甲苯（III）的混合溶液。 1-4％表面活性剂。 （II）:( III）的混合比为3:7）; （b）搅拌混合物。 以300-1000rpm的速度形成乳液; （c）用100μm筛子过滤并用D.W洗涤; （d）用戊二醛交联，干燥得到最终产物。 （I）的直径为150〜500μm，（I）的孔径为20〜50μm。
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公开(公告)号：KR1020120038030A
公开(公告)日：2012-04-23
申请号：KR1020100097335
申请日：2010-10-06
Applicant: 한국과학기술원
CPC classification number: C12P7/18
Abstract: PURPOSE: An arabinose metabolic pathway-introduced xylitol-producing strain and a method for producing xylitol using the same are provided to suppress arabitol generation. CONSTITUTION: A xylitol-producing strain(deposit number KCTC 11761BP) with suppressed arabitol generation is prepared by introducing a gene expression cassette containing an arabinoser metabolic pathway-associated gene into Candida sp. A method for procuring the strain comprises: a step of preparing a gene expression cassette having a terminator, a promoter, and a polynucleotide encoding L-arabinose isomerase; a step of preparing a gene having a promoter, a terminator, and a polynucleotide encoding L-ribrokinase; and a step of preparing a gene expression cassette having a promoter, a terminator, and a polynucleotide encoding L-ribrose-5-phosphate 4-epimerase.
Abstract translation: 目的：提供一种阿拉伯糖代谢途径引入的木糖醇生产菌株及使用其生产木糖醇的方法以抑制阿糖醇的产生。 构成：通过将含有阿拉伯糖代谢途径的基因的基因表达盒引入假丝酵母属（Candida sp。）制备具有抑制的阿拉伯糖醇生成的木糖醇生产菌株（保藏号KCTC 11761BP）。 获得该菌株的方法包括：制备具有终止子，启动子和编码L-阿拉伯糖异构酶的多核苷酸的基因表达盒的步骤; 制备具有启动子，终止子和编码L-核糖激酶的多核苷酸的基因的步骤; 以及制备具有启动子，终止子和编码L-核糖糖-5-磷酸4-差向异构酶的多核苷酸的基因表达盒的步骤。
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公开(公告)号：KR1020110120843A
公开(公告)日：2011-11-04
申请号：KR1020110041180
申请日：2011-04-29
Applicant: 한국과학기술원
IPC: G01N33/533 , G01N33/68 , G01N33/52
CPC classification number: G01N33/746 , C12N15/85 , G01N33/6803 , G01N33/6842 , G01N2400/02
Abstract: PURPOSE: A kit comprising a labeled antibody, a fluorescence-labeled biosubstance, and a support is provided to analyze the content of specific carbohydrate. CONSTITUTION: A kit for glycoprotein analysis comprises: a fluorescence-labeled antibody which selectively binds to a protein region of the glycoprotein; a florescence-labeled biosubstance which selectively binds to carbohydrate region of the glycoprotein; and a support which binds to the glycoprotein. The biosubstance is lectin, antibody, aptamer, or peptides. The glycoprotein is purified glycoprotein or isolated from single cells. The support is coated with other antibodies which bind to the glycoprotein targeting the surface.
Abstract translation: 目的：提供包含标记抗体，荧光标记的生物物质和载体的试剂盒，以分析特定碳水化合物的含量。 构成：用于糖蛋白分析的试剂盒包括：选择性结合糖蛋白的蛋白质区域的荧光标记抗体; 选择性结合糖蛋白的碳水化合物区域的荧光标记的生物物质; 和与糖蛋白结合的载体。 生物体是凝集素，抗体，适体或肽。 糖蛋白是纯化的糖蛋白或从单细胞分离。 该载体用与靶向该表面的糖蛋白结合的其它抗体包被。
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公开(公告)号：KR1020110080138A
公开(公告)日：2011-07-12
申请号：KR1020110059736
申请日：2011-06-20
Applicant: 한국과학기술원
Abstract: PURPOSE: A method for preparing xylitol of high yield in a medium containing glucose using a xylitol producing strain is provided to produce xylitol of high yield and high productivity without glucose suppression. CONSTITUTION: A gene construct contains a promoter containing a base of sequence number 1, a polynucleotide having a base of sequence number 7, and a terminator having a base of sequence number 2. The gene construct contains a promoter containing 1000bp or more base sequences, a polynucleotide having a base of sequence number 7, and a terminator having a base of sequence number 2. An expression vector contains one or more gene constructs. A method for producing a large amount of xylitols comprises: a step of culturing a xylotol-producing strain in a medium containing biomass hydrolysate and carbon source; a step of producing xylitol from the strain; and a step of isolating xylitol from the culture liquid.
Abstract translation: 目的：提供使用木糖醇生产菌株在含葡萄糖的培养基中制备高产率的木糖醇的方法，以生产高产率和高生产率的木糖醇而不需要葡萄糖抑制。 构成：基因构建体含有启动子，其含有序列号1的碱基，具有序列号7的碱基的多核苷酸和具有序列号2的碱基的终止子。该基因构建体含有含有1000bp或更多碱基序列的启动子， 具有序列号7的碱基的多核苷酸和具有序列号2的碱基的终止子。表达载体含有一种或多种基因构建体。 一种生产大量木糖醇的方法包括：在含有生物质水解物和碳源的培养基中培养产生木糖醇的菌株的步骤; 从菌株中生产木糖醇的步骤; 和从培养液中分离木糖醇的步骤。
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公开(公告)号：KR100730315B1
公开(公告)日：2007-06-19
申请号：KR1020050079751
申请日：2005-08-30
Applicant: 한국과학기술원
IPC: C12N1/16
Abstract: 본 발명은 자일리톨 탈수소효소의 발현이 억제된 자일리톨 생산균주를 이용하여 자일리톨을 고수율로 생산하는 방법에 관한 것이다. 본 발명의 자일리톨의 고수율 생산방법은 자일리톨 탈수소효소 유전자의 일부가 비특이적으로 결실, 치환 또는 부가되어 자일리톨 탈수소효소의 발현이 억제된 자일리톨 생산균주를 자일리톨의 전구체인 자일로스, 탄소원, 질소원 및 미량원소를 포함하는 배지에 접종하고, 배지에 함유된 자일로스가 전량 소모될 때까지 배양한 다음, 이로부터 자일리톨을 수득하는 단계를 포함한다. 본 발명의 자일리톨의 고수율 생산방법을 이용할 경우, 종래의 제조방법보다도 높은 수율로 자일리톨을 생산할 수 있었으므로, 자일리톨을 포함하는 각종 식품, 의약품 등의 제조에 널리 활용될 수 있을 것이다.
자일리톨, 자일로스, 자일리톨 탈수소효소, 자일리톨 생산균주-
Patent Agency Ranking
公开(公告)号：KR1020030016802A
公开(公告)日：2003-03-03
申请号：KR1020010050545
申请日：2001-08-22
IPC: A61K38/17
Abstract: PURPOSE: A composition of treating diarrhea in mammals containing buforin II which is separated a Korean toad and has an antimicrobial effect against cause bacteria of diarrhea is provided. The composition exhibits high susceptibility against cause bacteria of diarrhea and kills cause bacteria by inhibition of intracellular physiological action of the diarrhea cause bacteria. CONSTITUTION: The composition for prevention and treatment of diarrhea contains 0.01 to 20% by weight of buforin II, based on the total weight of the composition. The composition is powder, a drug, a tablet or injectable formulation and can be also used as a vaccine preparation, a feed additive or a beverage additive. Daily dose of between about 400μg to 4mg/day is sufficient to treat diarrhea in mammals.
Abstract translation: 目的：提供含有buforin II的哺乳动物中腹泻的组合物，其分离为韩国蟾蜍，并且具有抵抗引起腹泻细菌的抗微生物作用。 该组合物对引起腹泻细菌的易感性高，通过抑制腹泻引起细菌的细胞内生理作用而杀死细菌。 构成：基于组合物的总重量，用于预防和治疗腹泻的组合物含有0.01-20重量％的buforin II。 组合物为粉末，药物，片剂或可注射制剂，也可用作疫苗制剂，饲料添加剂或饮料添加剂。 约400μg至4mg /天的日剂量足以治疗哺乳动物的腹泻。
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公开(公告)号：KR1020030016801A
公开(公告)日：2003-03-03
申请号：KR1020010050544
申请日：2001-08-22
IPC: A61K38/17
Abstract: PURPOSE: A composition of treating mastitis in mammals containing buforin II having an antimicrobial effect against microorganism causing mastitis of a lactating cow is provided. The composition is excellent in stability as well as mastitis treating effect, inhibits generation of antibiotic resistant strains of bacteria and repels cause bacteria. CONSTITUTION: The mastitis treating composition contains 0.01 to 20% by weight of buforin II and additionally an excipient, based on the total weight of the composition. The excipient is one or more selected from the group consisting of an aqueous ointment base material, oily ointment base material, olive oil and white bee'wax. Daily dosages of between about 8 and 80mg/day are sufficient to treat mastitis in mammals.
Abstract translation: 目的：提供一种哺乳动物治疗乳腺炎的组合物，其含有对引起乳牛的乳腺炎的微生物具有抗微生物作用的buforin II。 该组合物的稳定性以及乳腺炎治疗效果优异，抑制抗生素抗性菌株的产生并排斥引起细菌。 构成：乳房炎治疗组合物含有0.01至20重量％的buforin II，另外还包含赋形剂，基于组合物的总重量。 赋形剂是选自水性软膏基材，油性软膏剂基料，橄榄油和白色蜂蜡中的一种以上。 约8至80mg /天的日剂量足以治疗哺乳动物的乳腺炎。
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公开(公告)号：KR101488778B1
公开(公告)日：2015-02-06
申请号：KR1020130032288
申请日：2013-03-26
Applicant: 한국과학기술원
Abstract: 본 발명은 자일리톨 탈수소효소(xylitol dehydrogenase,
XDH )가 제거된 기존의 변이주에서 자일룰로스 키나아제(xylulose kinase,
XK )를 추가적으로 제거한 생산균주 및 이를 이용하여 반복적인 발효공정에도 높은 수율을 나타내는 자일리톨 생산방법에 관한 것이다. 구체적으로, 자일룰로스 키나아제 유전자의 활성이 제거된 균주를 선별하였으며, 선별된 균주의 반복적인 발효실험을 통해 균주의 발효 생리학적 특성을 분석하여 공정을 최적화하고, 반복적인 발효공정에도 높은 수율을 유지할 수 있는 균주 및 공정을 확립함으로써, 본 발명의 자일리톨 생산균주 및 생산방법이 고수율, 고생산성으로 자일리톨을 생산하는데 유용하게 사용될 것이다.-
公开(公告)号：KR1020110078548A
公开(公告)日：2011-07-07
申请号：KR1020090135380
申请日：2009-12-31
Applicant: 한국과학기술원
Abstract: PURPOSE: A method for preparing xylitol at a high yield by the constitutive expression of xylose reductase is provided to massively produce xylitol. CONSTITUTION: A gene construct comprises: a promoter having a base sequence of sequence number 1; a polynucleotide having a base sequence of sequence number 7; and a terminator having a base sequence of sequence number 2. A strain which produces xylitol is cultured in a medium containing biomass hydrolysate and carbon source. The biomass hydrolysate is selected from the group consisting of corncob hydrolysate, sugar cane cob hydrolysate, coconut by-product, and Betula platyphylla var. japonica (Miquel) Hara hydrolysate.
Abstract translation: 目的：提供通过木糖还原酶的组成型表达以高产率制备木糖醇的方法，以大量生产木糖醇。 构成：基因构建体包含：具有序列号1的碱基序列的启动子; 具有序列号7的碱基序列的多核苷酸; 和具有序列号2的碱基序列的终止子。产生木糖醇的菌株在含有生物质水解产物和碳源的培养基中培养。 生物质水解物选自玉米芯水解产物，甘蔗棒状水解产物，椰子副产物和Betula platyphylla var。 粳米（Miquel）Hara水解产物。
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公开(公告)号：KR1020110072732A
公开(公告)日：2011-06-29
申请号：KR1020090129789
申请日：2009-12-23
Applicant: 한국과학기술원
IPC: G01N33/574 , G01N33/535 , G01N33/533
Abstract: PURPOSE: A method for diagnosing colon cancer is provided to detect alpha 1-3/4 fucose level using AAL lectin and to enable simple diagnose of colon cancer. CONSTITUTION: A method for diagnosing colon cancer comprises: a step of pretreating alpha 1-6 fucosidase to test sample-derived blood; a step of treating the blood to an anti-beta-haptoglobin antibody-conjugated solid substrate and washing; a step of treating with AAL(Aleuria aurantia agglutinin) lectin on the substrate and washing; and a step of determining colon cancer in case that the AAL lectin attachment level is increased comparing the level of healthy person.
Abstract translation: 目的：提供一种诊断结肠癌的方法，以使用AAL凝集素检测α1-3/ 4岩藻糖水平，并能够简单诊断结肠癌。 构成：诊断结肠癌的方法包括：预处理α1-岩藻糖苷酶以测试样品来源的血液的步骤; 将血液处理为抗β-触珠蛋白抗体缀合的固体底物并洗涤的步骤; 在基质上洗涤AAL（Aleuria aurantia agglutinin）凝集素的步骤; 如果AAL凝集素附着水平与健康人的水平相比增加，则确定结肠癌的步骤。
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