公开(公告)号：AU2003258667A1

公开(公告)日：2004-04-30

申请号：AU2003258667

申请日：2003-08-26

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  KROGER BURKHARD

IPC: A23K1/00 ,  A23K1/16 ,  C12N15/09 ,  C12P13/04 ,  C12P13/12 ,  C12R1/15

Abstract: Fermentative production of at least one sulfur-containing fine chemical (I) by fermenting a (I)-producing coryneform bacterium (A), concentrating (I) in medium or cells, then isolating it. (A) expresses at least one heterologous nucleotide sequence (II) that encodes a protein (III) with methionine synthase (metF) activity. An Independent claim is also included for preparation of a feed additive (B) that contains L-methionine by culturing/fermenting an L-Met producing microorganisms, removing water from the fermentation broth and optionally also up to 100 % of the biomass, then drying the treated broth to recover (B) in powdered or granular form.

公开(公告)号：AU2003238385A1

公开(公告)日：2003-12-12

申请号：AU2003238385

申请日：2003-05-23

Applicant: BASF AG

Inventor： SCHRODER HARTWIG ,  KROGER BURKHARD ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HAFNER STEFAN

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12P13/12 ,  C12R1/15 ,  C12P11/00

Abstract: The invention relates to methods for the production of sulfur-containing fine chemicals, in particular L-methionine, by fermentation using bacteria in which a nucleotide sequence encoding an S-adenosylmethionine synthase (metK) gene is expressed.

公开(公告)号：AU2003242537A8

公开(公告)日：2003-12-02

申请号：AU2003242537

申请日：2003-05-13

Applicant: BASF AG

Inventor： KROGER BURKHARD ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  HAFNER STEFAN ,  LEE HEUNG-SHICK ,  ZELDER OSKAR

IPC: C12N15/09 ,  C07K14/34 ,  C12N15/31 ,  C12P13/08

Abstract: Isolated polypeptide sequence having the sequence of SEQ ID NO:1 or muteins thereof having the ability to bind cAMP and repress the expression of the aceB gene of C. glutamicum and which can be obtained from SEQ ID NO:1 by inserting, deleting or substituting up to 20% of the amino acids.

公开(公告)号：BRPI0612900A2

公开(公告)日：2014-10-14

申请号：BRPI0612900

申请日：2006-07-18

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  YOCUM R ROGERS ,  ZELDER OSKAR ,  SCHROEDER HARTWIG ,  WILLIAMS MARK K

IPC: C12P13/12

Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a &Dgr;metF organism or a &Dgr;metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A &Dgr;metF &Dgr;metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

公开(公告)号：BRPI0520842A2

公开(公告)日：2012-07-03

申请号：BRPI0520842

申请日：2005-12-21

Applicant: BASF AG

Inventor： HAEFNER STEFAN ,  SCHROEDER HARTWIG ,  ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  HEROLD ANDREA

IPC: C12N15/77 ,  C07K14/34

Abstract: An expression unit (A) that contains several promoters is new. A new expression unit (A) comprises several promoters and includes, in the 5' to 3' direction the sequence module (I). 5'-P 1-(A x-P x) n-A y-P y-3' (I) n = integer 0-10; A x and A y = same or different chemical bonds or nucleic acid linker sequences; P 1, P x and P y = same or different promoter sequences, containing at least one RNA-polymerase binding region, and at least P y also includes a ribosome-binding, 3'-terminal segment. Independent claims are also included for: (1) an expression cassette (EC) that contains, in the 5' to 3' direction, the sequence module (II); (2) vector that contains at least one EC; (3) a genetically modified microorganism (GMO) transfected with the vector of (2) or containing EC; and (4) method for preparing biosynthetic products (X) by culturing GMO. 5'-P 1-(A x-P x) n-A y-P y-G-3' (II) G : at least one nucleic acid coding sequence functionally linked to the 5'-upstream regulatory sequence.

公开(公告)号：BR0216047A

公开(公告)日：2010-09-14

申请号：BR0216047

申请日：2002-11-11

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  POMPEJUS MARKUS ,  SCHRÖDER HARTWIG ,  KRÖGER BURKHARD ,  KLOPPROGGE CORINNA ,  HABERHAUER GREGOR

IPC: C12N1/21 ,  C12N9/04 ,  C12N15/53

Abstract: Isolated nucleic acid (I) that encodes a glucose-6-phosphate dehydrogenase (II) from Corynebacterium glutamicumin which Ala at position 243 has been replaced by some other amino acid, specifically Thr, is new. Independent claims are also included for the following: (1) Vector (V) that contains (I); (2) Host cell (C) transfected with (V); and (3) Preparing fine chemicals by culturing (C).

公开(公告)号：IN387CH2008A

公开(公告)日：2008-09-19

申请号：IN387CH2008

申请日：2008-01-23

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  KIEFER PATRICK ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/16 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：IN2603CH2006A

公开(公告)日：2007-06-08

申请号：IN2603CH2006

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  KIEFER PATRICK ,  HAEFNER STEFAN ,  KROEGER BURKHARD ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12N9/16 ,  C12P13/08

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.

公开(公告)号：BRPI0417726A

公开(公告)日：2007-04-03

申请号：BRPI0417726

申请日：2004-12-16

Applicant: BASF AG

Inventor： KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  GER BURKHARD KR ,  DER HARTWIG SCHR ,  HAEFNER STEFAN ,  RUFFER UWE ,  GRAEF CLAUDIA ISABELLA ,  HABERHAUER GREGOR

IPC: C07K14/195 ,  C07K14/34

Abstract: The present invention relates to mutated nucleic acids and proteins of the metabolic pathway of fine chemicals, to processes for preparing genetically modified producer organisms, to processes for preparing fine chemicals by culturing genetically modified organisms, and to genetically modified organisms themselves.

公开(公告)号：BRPI0417706A

公开(公告)日：2007-03-20

申请号：BRPI0417706

申请日：2004-12-17

Applicant: BASF AG

Inventor： ZELDER OSKAR ,  KLOPPROGGE CORINNA ,  SCHROEDER HARTWIG ,  HAEFNER STEFAN ,  KRUEGER BURKHARD ,  KIEFER PATRICK ,  HEINZLE ELMAR ,  WITTMANN CHRISTOPH

IPC: C12P20060101 ,  C12P13/08 ,  C12N9/12 ,  C12N15/54 ,  C12N1/21

Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i. e., glycerol kinase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of glycerol kinase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.