中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

45 records (took 0.024 seconds)

    Stable NAD/NADH derivatives
    43.
    发明专利
    Stable NAD/NADH derivatives 未知

    公开(公告)号：AU2011200465A1

    公开(公告)日：2011-02-24

    申请号：AU2011200465

    申请日：2011-02-04

    Applicant: HOFFMANN LA ROCHE

    Inventor： HORN CARINA ,  GAESSLER-DIETSCHE CLAUDIA ,  HEINDL DIETER ,  HOENES JOACHIM

    IPC: C07H19/207 ,  C07H19/213 ,  C07H21/00 ,  C07H21/02 ,  C07H23/00 ,  C12Q1/32

    Abstract: Test element for the determination of an analyte, comprising a coenzyme dependent enzyme or a substrate of the enzyme, and a coenzyme compound (I) and its salt or optionally its reduced form, is new. Test element for the determination of an analyte, comprises a coenzyme dependent enzyme or a substrate of the enzyme, and a coenzyme of pyridine compound of formula (I) and its salt or optionally its reduced form. A : adenine or their analog; T : O or S; U 1> : OH, SH, BH 3 -> or BCNH 2 ->; V 1> : OH or phosphate group; W 1> : COOR, CON(R) 2, COR, CSN(R) 2; R : H or 1-2C-alkyl; X 1, X 2 : O, CH 2, CHCH 3, C(CH 3) 2, NH or NCH 3; Y 1> : NH, S, O or CH 2; Z : cyclic group with 5C atom optionally substituted with heteroatom of O, S or N and CR 4, where C(R 4) 2 is a cyclic group with X 2; and R 4 : H, F, Cl or CH 3. Where Z and pyridine are not connected by a glycosidic bond. Independent claims are included for: (1) a pyridine compound (I); (2) an enzyme-coenzyme-complex comprising (I) in combination with an enzyme; (3) a reagent kit comprising (I), optionally an enzyme or enzyme complex and optionally a reaction buffer; and (4) a method for the determination of analyte comprising contacting the sample with the test element or reagent kit comprising coenzyme, and detecting the analyte. [Image].

    45.
    发明专利
    未知

    公开(公告)号：AT412772T

    公开(公告)日：2008-11-15

    申请号：AT06022257

    申请日：2006-10-24

    Applicant: HOFFMANN LA ROCHE

    Inventor： PETRICH WOLFGANG ,  GAESSLER-DIETSCHE CLAUDIA ,  HORN CARINA ,  HEBESTREIT KAI ,  ASFOUR JEAN-MICHEL ,  BARDELANG KLEMENS ,  KOCHERSCHEIDT GERRIT

    IPC: C12Q1/54 ,  C12Q1/00 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/52 ,  G01N33/66

    Abstract: Procedure for the detection of an analyte in a sample by fluorescence measurement, comprises providing a detection medium containing the sample, optionally at least an analyte detecting reagent, a fluorophore or its preliminary component, an absorber that absorbs light over a part of the excitation range of the fluorophore; irradiating the medium with light for the excitation of the fluorophore within the range of the second wavelength; and determining the fluorescence emission of the fluorophore in one or more measuring wavelengths. Procedure for the detection of an analyte in a sample by fluorescence measurement, comprises providing a detection medium containing at least a part of the sample, in which the analyte has to be detected, optionally at least a reagent for the detection of the analyte, a fluorophore that possesses an excitation range with at least an excitation maximum with a first wavelength, or a fluorophore preliminary component, from which the fluorophore is formed in the presence of the sample and optionally the reagent, an absorber that absorbs light over a part of the excitation range of the fluorophore; irradiating the medium with light for the excitation of the fluorophore within the range of the second wavelength; and determining the fluorescence emission of the fluorophore in one or more measuring wavelengths to determine the quantity or activity of the analyte in the sample. Independent claims are included for: (1) a test element, for the detection of an analyte, comprising the fluorophore, absorber and optionally at least a reagent for the detection of an analyte, where the fluorophore and/or fluorophore preliminary component and absorber are located on the test element in such a manner that the irradiated light for the excitation of the fluorophore comes in contact at first with the absorber and then with the fluorophore or meets both the absorber and the fluorophore simultaneously, and the system from fluorophore and absorber results in an effectively changed excitation maximum with a second wavelength that is different from the first; and (2) use of a test element in a procedure, for the detection of an analyte in a sample, comprising contacting the test element with the sample, irradiating the sample with a light for the excitation of the fluorophore within the range of the second wavelength, and determining the fluorescence emission of the fluorophore.

Patent Agency Ranking