公开(公告)号：DE112005003135T5

公开(公告)日：2008-04-30

申请号：DE112005003135

申请日：2005-12-21

Applicant: INTEL CORP

Inventor： YAMAKAWA MINEO

IPC: G01N33/68

Abstract: Methods for analyzing a protein are provided, including isolating the protein having original reactive functional groups from a biological sample, and converting substantially all original reactive functional groups into thiol groups, followed by attaching tags to the protein via the thiol groups.

公开(公告)号：AT386822T

公开(公告)日：2008-03-15

申请号：AT02799571

申请日：2002-09-05

Applicant: INTEL CORP

Inventor： RAO VALLURI ,  NEUBAUER GABI ,  KIRCH STEVEN ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: C12Q1/68 ,  B01L3/00 ,  C07H21/02 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12N15/09 ,  C12P19/34 ,  G01J3/42 ,  G01J3/427 ,  G01J3/44 ,  G01N21/65 ,  G01N33/53 ,  G01N33/566

Abstract: The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

公开(公告)号：HK1093553A1

公开(公告)日：2007-03-02

申请号：HK07100264

申请日：2007-01-08

Applicant: INTEL CORP

Inventor： RAO VALLURI ,  MA QING ,  YAMAKAWA MINEO ,  BERLIN ANDREW ,  WANG LI-PENG ,  ZHANG YUEGANG

IPC: G01N20060101 ,  G01N29/02 ,  G01N29/036 ,  G01N29/34 ,  G01N33/543

公开(公告)号：AU2003303106A8

公开(公告)日：2004-08-13

申请号：AU2003303106

申请日：2003-11-26

Applicant: INTEL CORP

Inventor： YAMAKAWA MINEO ,  BERLIN ANDREW ,  RAO VALLURI

IPC: B01J19/00 ,  B05D3/00 ,  C12M1/34 ,  C12Q1/68 ,  G03F1/00 ,  G03F7/20

Abstract: A method and apparatus for forming a polymer array on a substrate suitable for synthesizing polymer sequences. This includes forming an array, each location of the array having at least one strand end, forming photosensitive protection on the strand ends, and selectively scanning and modulating at least one energy beam to expose a pattern on the photosensitive protection. In some embodiments, the method further includes removing a protective group from selected strand ends based on the exposed pattern. The method then includes adding a predetermined one or more polymeric subunits to the deprotected strand ends. In some embodiments the photosensitive protection includes a layer of photoresist to cover the strand ends. Some embodiments use an ultra-violet laser.

公开(公告)号：AU2003278774A1

公开(公告)日：2004-04-30

申请号：AU2003278774

申请日：2003-09-05

Applicant: INTEL CORP

Inventor： SUNDARARAJAN NARAYANAN ,  YAMAKAWA MINEO ,  HECK JOHN ,  CHAN SELENA

IPC: G01N33/483 ,  B01D63/08 ,  B01D71/02 ,  B01J19/00 ,  B01L3/00 ,  B81B1/00 ,  B81C1/00 ,  B81C3/00 ,  B82B3/00 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00

Abstract: Microfluidic apparatus including integrated porous substrate/sensors that may be used for detecting targeted biological and chemical molecules and compounds. In one aspect, upper and lower microfluidic channels are defined in respective halves of a substrate, which are sandwiched around a porous membrane upon assembly. In other aspect, the upper and lower channels are formed such that a portion of the lower channel passes beneath a portion of the upper channel to form a cross-channel area, wherein the membrane is disposed between the two channels. In various embodiments, one or more porous membranes are disposed proximate to corresponding cross-channel areas defined by one or more upper and lower channels. The porous membrane may also have sensing characteristics, such that it produces a change in an optical and/or electronic characteristic. Accordingly, the apparatus may further include instrumentation or detection equipment to measure the changes, such as optic-based detectors and electronic instrumentation.

公开(公告)号：AU2002323494A1

公开(公告)日：2003-04-07

申请号：AU2002323494

申请日：2002-08-30

Applicant: INTEL CORP

Inventor： YAMAKAWA MINEO ,  KIRCH STEVEN ,  RAO VALLURI ,  BERLIN ANDREW ,  NEUBAUER GABI

IPC: G01N21/65 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12N15/00 ,  C12Q1/44 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N37/00 ,  B01J19/00

公开(公告)号：DE60330809D1

公开(公告)日：2010-02-11

申请号：DE60330809

申请日：2003-10-07

Applicant: INTEL CORP

Inventor： CHAN SELENA ,  BERLIN ANDREW ,  KWON SUNGHOON ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO

IPC: G01J3/44 ,  B01D67/00 ,  B01D71/02 ,  C12Q1/68 ,  G01N21/65

Abstract: The methods, systems 400 and apparatus disclosed herein concern metal 150 impregnated porous substrates 110, 210. Certain embodiments of the invention concern methods for producing metal-coated porous silicon substrates 110, 210 that exhibit greatly improved uniformity and depth of penetration of metal 150 deposition. The increased uniformity and depth allow improved and more reproducible Raman detection of analytes. In exemplary embodiments of the invention, the methods may comprise oxidation of porous silicon 110, immersion in a metal salt solution 130, drying and thermal decomposition of the metal salt 140 to form a metal deposit 150. In other exemplary embodiments of the invention, the methods may comprise microfluidic impregnation of porous silicon substrates 210 with one or more metal salt solutions 130. Other embodiments of the invention concern apparatus and/or systems 400 for Raman detection of analytes, comprising metal-coated porous silicon substrates 110, 210 prepared by the disclosed methods.

公开(公告)号：GB2423579B

公开(公告)日：2008-04-09

申请号：GB0609804

申请日：2004-10-18

Applicant: INTEL CORP

Inventor： KOO TAE-WOONG ,  GERTH CHRISTOPHER ,  YAMAKAWA MINEO

IPC: G01J3/44 ,  G01N21/65

公开(公告)号：AU2003223269B2

公开(公告)日：2008-01-17

申请号：AU2003223269

申请日：2003-03-11

Applicant: INTEL CORP

Inventor： SU XING ,  KOO TAE-WOONG ,  SUNDARARAJAN NARAYAN ,  YAMAKAWA MINEO ,  BERLIN ANDREW ,  CHAN SELENA

IPC: G01N21/65 ,  B01L3/00 ,  C12M1/00 ,  C12Q1/68 ,  G01N33/543

Abstract: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13 . Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130, which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERFS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing.

公开(公告)号：DE60309903T2

公开(公告)日：2007-10-18

申请号：DE60309903

申请日：2003-09-24

Applicant: INTEL CORP

Inventor： SU XING ,  CHAN SELENA ,  KOO TAE-WOONG ,  YAMAKAWA MINEO ,  BERLIN ANDREW

IPC: G01N33/543 ,  C12Q1/68 ,  G01B7/34

Abstract: The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.