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公开(公告)号：KR1020150081774A
公开(公告)日：2015-07-15
申请号：KR1020140001589
申请日：2014-01-07
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6816 , C12N15/11 , C12Q1/686
Abstract: 본발명은 19(서열번호 1 및 2), 28(서열번호 3 및 4), B2-3(서열번호 5 및 6), B2-26(서열번호 7 및 8), B2-28(서열번호 9 및 10), B2-42(서열번호 11 및 12), B2-48(서열번호 13 및 14) 및 B2-55(서열번호 15 및 16)로구성되는군으로부터선택되는 1종이상의프라이머쌍을사용하는뽕나무동정방법, 키트및 프라이머조성물에관한것이다. 본발명에따른뽕나무동정기술은외관상으로는용이하게구별되지않는뽕나무의유전형을보다정확하고신속하게구분할수 있기때문에, 뽕나무의중요한유전자개발, 농업적형질연구와신규품종이육성등을포함하는산업적유용성을높이는데상당히유용하다.
Abstract translation: 本发明涉及通过使用一种或多种引物组的桑树鉴定方法，试剂盒和引物组合，所述引物组选自包含：19（SEQ ID NO：1和2）的组; 28（SEQ ID NO：3和4）; B2-3（SEQ ID NO：5和6）; B2-26（SEQ ID NO：7和8）; B2-28（SEQ ID NO：9和10）; B2-42（SEQ ID NO：11和12）; B2-48（SEQ ID NO：13和14）; 和B2-55（SEQ ID NO：15和16）。 本发明的桑树鉴定技术能够更准确，迅速地分离出不容易通过外观识别的桑树的基因型。 因此，本发明对于提高工业可用性，包括开发桑树重要基因，研究农业性状和新品种的育种是非常有用的。
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公开(公告)号：KR1020140048379A
公开(公告)日：2014-04-24
申请号：KR1020120112845
申请日：2012-10-11
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/686 , C12Q2525/151
Abstract: The present invention relates to an identifying method of Cordyceps militaris using a PCR method; a primer used for the method; and a Cordyceps militaris identifying kit including the primer. By using the method of the present invention, a user can accurately and rapidly identify Cordyceps militaris which is not easily identified by the outer appearance, thereby capable of being widely applied for developing various products containing Cordyceps militaris.
Abstract translation: 本发明涉及使用PCR方法鉴定冬虫夏草的方法; 用于该方法的底漆; 和含有引物的冬虫夏草militaris鉴定试剂盒。 通过使用本发明的方法，用户可以准确而快速地鉴定出不易被外观识别的冬虫夏草，从而可广泛应用于开发含有冬虫夏草的各种产品。
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公开(公告)号：KR100847889B1
公开(公告)日：2008-07-25
申请号：KR1020070020818
申请日：2007-03-02
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12P19/04 , A23L33/10 , A23V2002/00 , A23V2200/318 , A23V2200/326 , A23V2200/332 , A61K31/726 , C08B37/0063
Abstract: Glycosaminoglycans derived from insect are provided to maintain the systolic blood pressure be normal level in a hypertension patient by reducing blood pressure, regulate excessive growth of vascular endothelial cell and inhibit inflammation without excess of heart and pulse pressure. A method for producing glycosaminoglycans derived from insect comprises the steps of: (1) dipping a dried insect in an organic solvent selected from acetone, hexane, ethylacetate, methylalcohol, ethylalcohol, propanol, butanol, acetonitrile and dichloromethane to remove lipids and drying it; (2) degrading the dried insect of step (1) with alcalase in sodium bicarbonate solution(pH 9.0); (3) removing degraded proteins with 5 wt.% of trichloroacetic acid(TCA), and precipitating the protein removed solution with ethanol, cetylpyridinium and ethanol, sequentially; and (4) dialyzing the precipitates produced from the step (3) with distilled water.
Abstract translation: 提供衍生自昆虫的糖胺聚糖以通过降低血压，调节血管内皮细胞的过度生长并抑制炎症而不会过多的心脏和脉搏压力，使高血压患者的收缩压维持正常水平。 一种生产来自昆虫的糖胺聚糖的方法，包括以下步骤：（1）将干燥的昆虫浸入选自丙酮，己烷，乙酸乙酯，甲醇，乙醇，丙醇，丁醇，乙腈和二氯甲烷的有机溶剂中以除去脂质并干燥; （2）用碳酸氢钠溶液（pH9.0）中的alcalase降解步骤（1）的干燥昆虫; （3）用5重量％的三氯乙酸（TCA）除去降解的蛋白质，并依次用乙醇，十六烷基吡啶和乙醇沉淀除去蛋白质的溶液; 和（4）用蒸馏水透析步骤（3）产生的沉淀物。
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公开(公告)号：KR100758813B1
公开(公告)日：2007-09-19
申请号：KR1020050133381
申请日：2005-12-29
Applicant: 대한민국(농촌진흥청장)
Abstract: A method for preserving pupa infected with Cordyceps spp, entomopathogenic fungi for a long time and a method for regenerating the fungi are provided to dispense with an expensive equipment or high technology for preserving the fungi, and also to produce fruit bodies on consumer demands by taking out the preserved pupa at desired time. The cultivation time of Cordyceps spp, entomopathogenic fungi can be controlled voluntarily, thereby solving the concentration of production time and the surplus or stock of production. A long-term preserving method of pupa infected with Cordyceps spp, entomopathogenic fungi comprises: preparing the pupa infected with Cordyceps spp, entomopathogenic fungi, controlling the water content of the pupa body to 4%, and then preserving the pupa at 4°C or less, or at -70°C after freeze-drying. The fungi regenerating method comprises soaking the preserved pupa in distilled water for 1~3hr, followed by saturating the pupa with water. The infected pupa can be preserved stably in a water content of 4% at a storage temperature of 4°C or less for 135day, and the regeneration ratio is to about 97%. In addition, a preservation period is more increased at lower temperature in freezing, and is up to 1year in preserving at -70°C or less after freeze-drying.
Abstract translation: 提供了一种用于保存长时间感染冬虫夏草，昆虫病原真菌的蛹的方法和用于再生真菌的方法，以省去用于保存真菌的昂贵的设备或高技术，以及通过采取消费者需求来生产果实 在所需的时间出来保存的蛹。 冬虫夏草，昆虫病原菌的培养时间可以自愿控制，从而解决生产时间和生产过剩或库存的集中。 昆虫病原真菌感染虫蛹的长期保存方法包括：制备感染冬虫夏草，昆虫病原真菌的蛹，将蛹体的水含量控制在4％，然后在4℃保存蛹，或 较少，或在-70℃下冷冻干燥。 真菌再生方法包括将保存的蛹在蒸馏水中浸泡1〜3小时，然后用水饱和蛹。 感染蛹可以在4℃或更低的储存温度下稳定保存在4％的水含量中，135天，再生率为约97％。 另外，冷冻时的温度保持时间更长，冷冻干燥后在-70℃以下保存1年左右。
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Patent Agency Ranking
公开(公告)号：KR100466522B1
公开(公告)日：2005-01-15
申请号：KR1020020045969
申请日：2002-08-03
Applicant: 대한민국(농촌진흥청장) , 학교법인 성균관대학
IPC: C07D209/36
Abstract: PURPOSE: Provided are an extract of Paecilomyces sp. J300 having antitumor activity and a pharmaceutical composition containing the same. The extract contains novel antitumor matters, and is thus useful for an antitumor drug composition. CONSTITUTION: A extract of Paecilomyces sp. J300 having antitumor activity contains, as antitumor matters, 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine represented by the formula(1) or its derivatives. An antitumor drug composition contains one of 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine or its derivatives including 5 alpha, 6 alpha-epoxy-(22E,24R)-ergosta-8(14), 22-diene-3 beta, 7 alpha-diol of the formula(2), 5 alpha, 6 alpha-epoxy-(22E, 24R)-ergosta-8, 22-diene-3 beta, 7 alpha-diol of the formula(3), ergosta-4, 6, 8(14), 22-tetraene-3-one of the formula(4) and 3 beta, 5 alpha-dihydroxy-6 beta-methoxyergosta-7, 22-diene of the formula(5).
Abstract translation: 用途：提供了拟青霉菌提取物。 具有抗肿瘤活性的J300及含有其的药物组合物。 提取物含有新的抗肿瘤物质，因此可用于抗肿瘤药物组合物。 组成：拟青霉菌提取物。 具有抗肿瘤活性的J300含有由式（1）表示的5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异黄嘌呤基甘氨酸或其衍生物作为抗肿瘤物质。 抗肿瘤药物组合物含有5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异黄嘌呤基甘氨酸或其衍生物，包括5α，6α-环氧 - （22E，24R） - 肉豆蔻酰 - 8（14），22-二烯-3β，式（2）的7α-二醇，5α，6α-环氧 - （22E，24R）-ergosta-8,22-二烯-3β，7α （3）的二醇，麦角甾-4,6,8（14），22-四烯-3-酮和3β，5α-二羟基-6β-甲氧麦角甾-7,22 （5）的二烯。
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公开(公告)号：KR1020040012399A
公开(公告)日：2004-02-11
申请号：KR1020020045969
申请日：2002-08-03
Applicant: 대한민국(농촌진흥청장) , 학교법인 성균관대학
IPC: C07D209/36
Abstract: PURPOSE: Provided are an extract of Paecilomyces sp. J300 having antitumor activity and a pharmaceutical composition containing the same. The extract contains novel antitumor matters, and is thus useful for an antitumor drug composition. CONSTITUTION: A extract of Paecilomyces sp. J300 having antitumor activity contains, as antitumor matters, 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine represented by the formula(1) or its derivatives. An antitumor drug composition contains one of 5,3-dihydroxy-1-N-methyl-indoline-2-carbonylamino-alanyl-hydoxyisoleucinyl-glycine or its derivatives including 5 alpha, 6 alpha-epoxy-(22E,24R)-ergosta-8(14), 22-diene-3 beta, 7 alpha-diol of the formula(2), 5 alpha, 6 alpha-epoxy-(22E, 24R)-ergosta-8, 22-diene-3 beta, 7 alpha-diol of the formula(3), ergosta-4, 6, 8(14), 22-tetraene-3-one of the formula(4) and 3 beta, 5 alpha-dihydroxy-6 beta-methoxyergosta-7, 22-diene of the formula(5).
Abstract translation: 目的：提供拟青霉菌属 J300具有抗肿瘤活性和含有其的药物组合物。 提取物含有新的抗肿瘤物质，因此可用于抗肿瘤药物组合物。 构成：拟青霉菌提取物 具有抗肿瘤活性的J300含有作为抗肿瘤物质的由式（1）表示的5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异丁烯基 - 甘氨酸或其衍生物。 一种抗肿瘤药物组合物包含5,3-二羟基-1-N-甲基 - 二氢吲哚-2-羰基氨基 - 丙氨酰 - 羟基异亮氨酰基甘氨酸或其衍生物之一，包括5α，6α-环氧 - （22E，24R） 8（14），22-二烯-3β，7α-二醇，5α，6α-环氧 - （22E，24R） - 麦角甾-8,22-二烯-3β，7α 式（3）的二醇，式（4）的麦角胺-4,6,8（14），22-四烯-3-酮和3β，5α-二羟基-6β-甲氧麦角甾-7,22 - 二烯（式（5））。
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公开(公告)号：KR1020030012998A
公开(公告)日：2003-02-14
申请号：KR1020010047271
申请日：2001-08-06
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
CPC classification number: A61K36/068
Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.
Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，从而大量生产Isaria sinclairii。 构成：使用家蚕培养伊蚊（Isaria sinclairii）的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹的表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中通过在浓度为106 / ml至108 / ml的水中稀释Isaria sinclairii的孢子进行接种过程，将稀释的溶液喷洒在 家蚕的幼虫或蛹2周，3周，4周和5周，并将其置于95％湿度下12小时; 家蚕的幼虫或蛹保存在20度。 C以下; 果实体形成处理在15〜25℃的条件下进行。 C和湿度95％。
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公开(公告)号：KR1020020067367A
公开(公告)日：2002-08-22
申请号：KR1020010007899
申请日：2001-02-16
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
CPC classification number: C12N1/14
Abstract: PURPOSE: Provided is a preparation method of a medium for Entomopathogenic fungi effectively and economically by using silkworm powder used as a hypoglycemic agent. The medium increases the growth rate of Entomopathogenic fungi. CONSTITUTION: The medium for Entomopathogenic fungi is characterized by dissolving 24g of silkworm powder in 1L of distilled water; sterilizing it at 121 deg.C for 15 minutes; filtering the sterilized solution with gauze and a sieve sequentially; adding 15g of agar the pure solution; sterilizing it; and pipetting 21mL of the solution into each petridish the solidifying them.
Abstract translation: 目的：通过使用用作降血糖药的蚕粉，有效和经济地提供了一种用于昆虫病原真菌的培养基的制备方法。 培养基增加了真菌致病真菌的生长速度。 构成：昆虫病原真菌的特征是将24g蚕粉溶解在1L蒸馏水中; 在121℃下灭菌15分钟; 用纱布和筛子依次过滤灭菌液; 加入15g琼脂纯溶液; 消毒; 并将21mL的溶液移液到每个头皮中固化。
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公开(公告)号：KR100187897B1
公开(公告)日：1999-06-01
申请号：KR1019960062054
申请日：1996-12-05
Applicant: 대한민국(농촌진흥청장)
IPC: A01G1/04
Abstract: 본 발명은 항암·면역증강 등 각종 효능이 뛰어난 것으로 알려져 있으나 자연에서의 채집이 극히 어려운 관계로 인간에게 유용하게 이용되지 못하고 있는 동충하초(Paecilomyces japonica)를 인공재배하는 방법에 관한 것으로, 동충하초의 일종인 페실로마이세스 자포니카균 포자를 분무기를 이용하여 가잠의 4∼5령기 유충 또는 번데기 표피에 경피 접종한 후, 균사체를 형성한 번데기에서 자실체를 형성시키는 것을 특징으로 한다.
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