公开(公告)号：KR101352035B1

公开(公告)日：2014-01-17

申请号：KR1020110067744

申请日：2011-07-08

Applicant: 대한민국(농촌진흥청장)

Inventor： 안일평 ,  김동헌 ,  배신철 ,  황덕주 ,  김민갑 ,  박상렬

IPC: C12N15/29 ,  C12N15/82 ,  C12N15/67 ,  A01H5/00

Abstract: 본 발명은 벼도열병 저항성 단백질, 이를 암호화하는 유전자, 상기 유전자를 포함하는 재조합 발현 벡터, 및 상기 재조합 발현 벡터로 형질전환된 형질전환체, 및 벼도열병 저항성 단백질의 세포내 수준을 조절하여 식물의 벼도열병 저항성을 조절하는 방법에 관한 것으로, 본 발명에 따르면 벼를 포함하는 식물을 벼도열병 저항성 품종육성으로 개발 가능하며 이로부터 농약사용절감과 함께 이로 인한 생산성향상 및 안전한 농산물공급이 가능할 것으로 기대된다.

公开(公告)号：KR1020140002907A

公开(公告)日：2014-01-09

申请号：KR1020120069757

申请日：2012-06-28

Applicant: 대한민국(농촌진흥청장)

Inventor： 안일평 ,  김진영 ,  김정분 ,  배신철 ,  황덕주 ,  박상렬

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/04

CPC classification number: C12Q1/6895 ,  C12Q1/04 ,  C12Q1/6851

Abstract: The present invention provides a composition for detecting Pyricularia oryzae, which includes a primer set and a probe with excellent specificity and sensitivity and a quantitative detection method of Pyricularia oryzae using the same. A composition for detecting Pyricularia oryzae according to the present invention can establish a molecular biological monitoring system of Pyricularia oryzae and objectively quantify an infection of Pyricularia oryzae.

Abstract translation: 本发明提供了一种用于检测稻瘟病菌的组合物，其包括引物组和具有优异的特异性和灵敏度的探针，以及使用该组合物的Pyricularia oryzae的定量检测方法。 根据本发明的用于检测稻瘟菌的组合物可以建立稻瘟病菌的分子生物学监测系统，并客观地定量米糠an的感染。

公开(公告)号：KR1020040051389A

公开(公告)日：2004-06-18

申请号：KR1020020079315

申请日：2002-12-12

Applicant: 대한민국(농촌진흥청장)

Inventor： 배신철 ,  황덕주 ,  변명옥 ,  고승주 ,  강권규 ,  권순종 ,  김호일

IPC: A01H4/00

CPC classification number: C12N15/8243 ,  C12N15/8205 ,  C12N15/8216

Abstract: PURPOSE: Iron fortified potato is provided, therein increasing the expression of the ferritin gene within potato, and resolving the iron deficient problems in food. CONSTITUTION: The method for producing the iron fortified potato comprises the steps of: connecting a ferritin gene of soybean to 35S CaMV vector; inserting PinII terminator into the 35S CaMV vector; transferring the 35S CaMV vector into pCAMBIA3300 binary vector; transforming potato with the pCAMBIA3300 binary vector containing the ferritin gene of soybean by co-culturing Agrobacterium tumefaciens LBA4404 with potato in MSO medium for 48 hours; inducing callus of infected potato in a callus inducing medium; and forming roots of infected potato in a root inducing medium.

Abstract translation: 目的：提供铁强化马铃薯，增加马铃薯内铁蛋白基因的表达，解决食物中缺铁问题。 构成：铁强化马铃薯的生产方法包括：将大豆铁蛋白基因连接到35S CaMV载体上; 将PinII终止子插入35S CaMV载体; 将35S CaMV载体转移到pCAMBIA3300二元载体中; 通过与MSO培养基中的马铃薯共培养根癌土壤杆菌LBA4404 48小时，通过含有大豆铁蛋白基因的pCAMBIA3300二元载体转化马铃薯; 在愈伤组织诱导培养基中诱导感染马铃薯的愈伤组织; 并在根诱导培养基中形成感染马铃薯的根。

公开(公告)号：KR100059359B1

公开(公告)日：1993-02-13

申请号：KR1019900011101

申请日：1990-07-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 황영수 ,  배신철 ,  최용문 ,  정태영 ,  김동수

IPC: C12Q1/68

公开(公告)号：KR1019920009516B1

公开(公告)日：1992-10-17

申请号：KR1019900011101

申请日：1990-07-21

Applicant: 대한민국(농촌진흥청장)

Inventor： 황영수 ,  배신철 ,  최용문 ,  정태영 ,  김동수

IPC: C12Q1/68

Abstract: A DNA probe for early diagnosing black line dwarf disease virus (I) is prepd. by (a) separating the (I) from a green leafhopper larva infected with the (I), (b) preparing cDNA library of the (I), (c) ligating the obtd. cDNA with rambda gtll vector to obtain the recombinant rambda gtll phage DNA (II), (d) inserting the obtd. (II) into pUC19 and transforming into E. coli JM101, and (e) collecting the recombinant clone. The obtd. DNA probe is useful for the early diagnosis of a green leafhopper larba infected with the (I).

Abstract translation: 用于早期诊断黑线矮病病毒（I）的DNA探针是制备的。 通过（a）将（I）与（I）感染的绿叶蝉幼虫分离，（b）制备（I）的cDNA文库，（c）连接该物质。 具有λgtll载体的cDNA以获得重组噬菌体DNA（II），（d）插入该载体。 （II）转化成pUC19并转化到大肠杆菌JM101中，（e）收集重组克隆。 有限公司 DNA探针可用于早期诊断感染（I）的绿叶蝉幼虫。