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公开(公告)号：KR1020130024815A
公开(公告)日：2013-03-08
申请号：KR1020120094285
申请日：2012-08-28
Applicant: 고려대학교 산학협력단
CPC classification number: C12N15/8246 , C07K14/415 , C12N15/8243 , C12N15/8255 , C12N15/8261 , C12P19/02 , C12P19/14 , Y02A40/146 , Y02E50/16
Abstract: PURPOSE: A wood transformed using RabG3bCA is provided to enhance the length of fibrous cells which determines the quality of pulp. CONSTITUTION: A wood is transformed with a RabG3b active type mutant, that is RabG3bCA. The RabG3bCA gene has a base of sequence number 1. RabG3b is derived from Arabidopsis thaliana. The transgenic wood has enhanced cellulose and glucose contents. The length of the transgenic wood is longer than the length of a wild type wood.
Abstract translation: 目的：提供使用RabG3bCA转化的木材，以增加确定纸浆质量的纤维细胞的长度。 构成：用RabG3b活性型突变体转化木材，即RabG3bCA。 RabG3bCA基因具有序列号1的碱基.RabG3b衍生自拟南芥。 转基因木材具有增强的纤维素和葡萄糖含量。 转基因木材的长度长于野生型木材的长度。
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公开(公告)号：KR1020130012863A
公开(公告)日：2013-02-05
申请号：KR1020110074288
申请日：2011-07-26
Applicant: 고려대학교 산학협력단
CPC classification number: C12N1/20
Abstract: PURPOSE: A novel marine microorganism which metabolizes 3,6-anhydro-galactose is provided to identify a metabolic pathway of 3,6-anhydro-galactose and to enhance the production yield of biofuel. CONSTITUTION: A novel marine microorganism Vibrio sp. EJY3(KCTC 11976BP) grows using 3,6-anhydro-L-galactose as a single carbon source. The novel strain produces an enzyme which metabolizes 3,6-anhydro-L-galactose. A composition which produces ribose through metabolism contains the strain. The composition contains the enzyme.
Abstract translation: 目的：提供代谢3,6-脱水半乳糖的新型海洋微生物，以鉴定3,6-脱水半乳糖的代谢途径，并提高生物燃料的产量。 构成：一种新型海洋微生物弧菌 EJY3（KCTC 11976BP）使用3,6-脱水-L-半乳糖作为单一碳源生长。 新菌株产生一种代谢3,6-脱水-L-半乳糖的酶。 通过代谢产生核糖的组合物含有菌株。 组合物含有酶。
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63.发明公开3,6-안하이드로-Ｌ-갈락토오스에 작용하는 신규한 3,6-안하이드로-Ｌ-갈락토오스 데히드로게나제와 이 효소를 이용한 3,6-안하이드로 갈락토닉산의 생산 有权将3,6-ANHYDRO-L-GALACTOSE转化为3,6-壬酰乳酸的NO3LE-3,6-ANHYDRO-L-GALACTOSE DEHYDROGENASE
公开(公告)号：KR1020120085364A
公开(公告)日：2012-08-01
申请号：KR1020110006631
申请日：2011-01-24
Applicant: 고려대학교 산학협력단
CPC classification number: C07D307/20 , C12N9/0006 , C12P17/04
Abstract: PURPOSE: A method for producing 3,6-anhydro galactonic acid using novel 3,6-anhydro-L-galactose dehydrogenase is provided to enhance bioenergy yield from marine algae. CONSTITUTION: A novel 3,6-anhydro-L-galactose dehydrogenase enzyme produces 3,6-anhydrogalactonic acid by oxidizing aldehyde group of the first carbon of 3,6-anhydro-L-galactose using NADP as a cofactor. The novel enzyme has an amino acid sequence of sequence numbers 1-4. A gene encoding 3,6-anhydro-L-galactose dehydrogenase has bases of sequence numbers 5-8. A recombinant vector contains the gene. A method for preparing 3,6-anhydro galatonic acid comprises: a step of reacting 3,6-anhydro-L-galactose dehydrogenase, a microorganism producing the novel enzyme, or a product of the microorganism with 3,6-anhydro-L-galactose; and a step of collecting the produced 3,6-anhydro galactonic acid.
Abstract translation: 目的：提供使用新型3,6-脱水-L-半乳糖脱氢酶生产3,6-脱水半乳糖酸的方法，以提高海藻生物能源产量。 构成：一种新型的3,6-脱水-L-半乳糖脱氢酶通过使用NADP作为辅因子来氧化3,6-脱水-L-半乳糖的第一碳的醛基而产生3,6-脱水半乳糖酸。 该新型酶具有序列号1-4的氨基酸序列。 编码3,6-脱水-L-半乳糖脱氢酶的基因具有序列号5-8的碱基。 重组载体含有该基因。 制备3,6-脱水镓半乳糖酸的方法包括：使3,6-脱水-L-半乳糖脱氢酶，产生新酶的微生物或微生物产物与3,6-脱水-L-半乳糖脱氢酶， 半乳糖; 和收集生成的3,6-脱水半乳糖酸的步骤。
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公开(公告)号：KR1020120077339A
公开(公告)日：2012-07-10
申请号：KR1020100139266
申请日：2010-12-30
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for transducing a gene into Saccharophagus degradans and overexpressing the gene is provided to enable industrial application for producing bioethanol. CONSTITUTION: A method for expressing a gene in Saccharophagus degradans comprises: a step of injecting a vector structure containing a promoter into the cells of Saccharophagus degradans; and a step of overexpressing the gene. The promoter is aga 16B promoter. The vector structure further contains the origin of replication, such as pBBR1 or pMB1. The vector structure also contains a recombinant DNA selection marker. The marker is a kanamycin, gentamycin, or Chlorampenychole resistant gene.
Abstract translation: 目的：提供一种将基因转导入酵母属降解物并过量表达该基因的方法，以使工业化生产生物乙醇。 构成：在酿酒酵母降解体中表达基因的方法包括：将含有启动子的载体结构注射到酵母属分解菌的细胞中的步骤; 以及过表达基因的步骤。 启动子是aga 16B启动子。 载体结构还包含复制起点，如pBBR1或pMB1。 载体结构还含有重组DNA选择标记。 标记物是卡那霉素，庆大霉素或氯安宁抗性基因。
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公开(公告)号：KR101096332B1
公开(公告)日：2011-12-20
申请号：KR1020090021670
申请日：2009-03-13
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 식물의 생장을 저해시키지 않고 효과적으로 식물에서의 플라보노이드의 생합성을 증대시킬 수 있는 배지 조성물에 관한 것으로, 바람직하게는 수크로스를 200 mM 내지 300 mM 함유하는 것을 특징으로 하는 식물의 플라보노이드 합성을 증대시키기 위한 배지 조성물 및 이를 이용한 플라보노이드의 생산 방법에 관한 것이다.
레몬 밤, 들깨, 플라보노이드, 안토시아닌, 수크로스, 식물호르몬-
Patent Agency Ranking
公开(公告)号：KR1020110088832A
公开(公告)日：2011-08-04
申请号：KR1020100008531
申请日：2010-01-29
Applicant: 고려대학교 산학협력단
CPC classification number: A61K8/97 , A61K36/53 , A61Q19/02 , Y10S514/844
Abstract: PURPOSE: A composition containing lemon balm extract is provided to suppress melanin synthesis and to ensure antioxidation and skin whitening. CONSTITUTION: A composition for improving skin whitening contains lemon balm extract as an active ingredient. The lemon balm is irradiated by UV ray for 10-30 days and is isolated using alcohol as a solvent. The composition is a cosmetic composition used in the form of skin lotion, skin softener, skin toner, astringent, pack, and soap. The composition contains an adjuvant and carrier.
Abstract translation: 目的：提供含有柠檬香膏提取物的组合物以抑制黑色素合成并确保抗氧化和皮肤美白。 构成：用于改善皮肤美白的组合物含有柠檬香膏提取物作为活性成分。 柠檬香脂用紫外线照射10-30天，并使用醇作为溶剂进行分离。 该组合物是以皮肤洗剂，皮肤柔软剂，皮肤调色剂，收敛剂，包装和肥皂的形式使用的化妆品组合物。 该组合物含有佐剂和载体。
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公开(公告)号：KR1020110069809A
公开(公告)日：2011-06-23
申请号：KR1020117008348
申请日：2009-09-11
Applicant: 고려대학교 산학협력단
IPC: C07K14/195 , C12N15/31 , C12N1/20 , C12R1/01
CPC classification number: C07K14/195 , C07K14/32 , C12N9/2437 , C12P19/14 , C12N9/2417
Abstract: PURPOSE: A prokaryote-derived expansin protein is provided to save the amount of enzyme and to be used in biopulping or biostoning. CONSTITUTION: A cellulose-degrading composition contains 0.01-0.05 FPU of cellulase and 200-400ug of prokaryote-derived expansin protein based on 1g of the composition. A method for the prokaryote-derived expansin comprises a step of searching prokaryote-derived proteins having structural similarity with plant-derived expansin; a step of cloning the prokaryote-derived protein; and a step of expressing the proteins from strains. The prokaryote is selected from the group consisting of Bacillus subtilis, Hahella chejuensis(KCTC 2396), Dictyostelium discoideum, Neosartorya fischeri, Aspergillus fumigatus, Aspergillus clavatus, and Aspergillus oryzae.
Abstract translation: 目的：提供原核细胞衍生的扩展蛋白，以节省酶的量并用于生物制浆或生物制药。 构成：基于1g组合物，纤维素降解组合物含有0.01-0.05FPU纤维素酶和200-400ug原核细胞衍生的膨胀蛋白。 原核细胞衍生的扩展蛋白的方法包括检测与植物来源的扩展蛋白具有结构相似性的原核生物衍生蛋白质的步骤; 克隆原核生物蛋白质的一个步骤; 以及从菌株中表达蛋白质的步骤。 原核生物选自枯草芽孢杆菌，哈氏乳杆菌（KCTC 2396），盘基网柄菌（Dictyostelium discoideum），烟粉虱（Neosartorya fischeri），烟曲霉（Aspergillus fumigatus），曲霉菌（Aspergillus clavatus）和米曲霉（Aspergillus oryzae）。
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公开(公告)号：KR1020100000043A
公开(公告)日：2010-01-06
申请号：KR1020080059379
申请日：2008-06-24
Applicant: 고려대학교 산학협력단
IPC: A23L3/3454 , A23L3/3418 , A23L3/3409 , A23L3/34
CPC classification number: A23L3/349 , A23L3/3418 , A23L3/3508
Abstract: PURPOSE: A sterilizing method using organic acid and supercritical fluids is provided to offer good sterilization effects, and to prevent quality degradation of products while improving economical efficiency of sterilization processes. CONSTITUTION: A sterilizing method using organic acid and supercritical fluids includes a step for dipping food to be sterilized in the organic acid, and a step for processing the dipped food with the supercritical fluids. The food is meat products. The meat product is one selected from a group consisting of pork, beef and chicken. The target microorganism of food is [food poisoning bacteria or enteric bacteria. The organic acid is lactic acid or acetic acid. The organic acid liquid includes organic acid of 2 ~ 4 volume %.
Abstract translation: 目的：提供使用有机酸和超临界流体的灭菌方法，提供良好的灭菌效果，防止产品质量下降，同时提高灭菌工艺的经济性。 构成：使用有机酸和超临界流体的灭菌方法包括将食品浸入有机酸中的步骤，以及用超临界流体处理浸渍食品的步骤。 食物是肉制品。 肉类产品选自猪肉，牛肉和鸡肉。 食物的目标微生物是[食物中毒细菌或肠道细菌。 有机酸是乳酸或乙酸。 有机酸液体含有2〜4体积％的有机酸。
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公开(公告)号：KR1020080027102A
公开(公告)日：2008-03-26
申请号：KR1020060129621
申请日：2006-12-18
Applicant: 고려대학교 산학협력단
Abstract: A method for screening bio-materials with anti-atherogenic activity is provided to screen the anti-atherogenic materials conveniently with low cost by quantifying a cell number of monocytes attached to vascular endothelium without directly determining the number by eyes or using isotopes. A method for screening bio-materials with anti-atherogenic activity comprises the steps of: (a) culturing vascular endothelium; (b) treating the cultured material with a candidate material with anti-atherogenic activity; (c) adding monocytes to the culture material of the step(b) and then culturing it; (d) removing the monocytes not coupled to the vascular endothelium by washing; and (e) measuring the binding of the vascular endothelium and the monocytes through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay(MTT assay). An anti-atherogenic composition comprises an extract of Lithospermum erythrorhizon as an effective ingredient.
Abstract translation: 提供一种筛选具有抗动脉粥样硬化活性的生物材料的方法，通过量化连接于血管内皮的单核细胞的细胞数而不用眼睛或使用同位素直接确定数量，便宜地以低成本筛选抗动脉粥样硬化材料。 用于筛选具有抗动脉粥样硬化活性的生物材料的方法包括以下步骤：（a）培养血管内皮; （b）用具有抗动脉粥样硬化活性的候选物质处理培养物; （c）向步骤（b）的培养物中加入单核细胞，然后培养; （d）通过洗涤除去未偶联于血管内皮的单核细胞; 和（e）通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物测定（MTT测定）测量血管内皮和单核细胞的结合。 抗动脉粥样硬化组合物包含作为有效成分的紫草皂甙提取物。
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公开(公告)号：KR1020070053827A
公开(公告)日：2007-05-28
申请号：KR1020050111514
申请日：2005-11-22
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 셀룰로스를 확장시키는 활성이 있는 애기장대 유래 익스팬신 단백질 유전자, 이를 포함하는 재조합 벡터 및 이를 이용한 대장균에서의 익스팬신 단백질의 발현 방법에 관한 것이다.
본 발명에 따르면, 미생물에서 과발현된 식물유래 재조합 익스팬신을 손쉽게 대량생산하는 방법을 개발함으로써 익스팬신 단백질을 세포벽의 주성분인 셀룰로스의 확장 활성을 조사하고 여러 가지 섬유소에 대한 셀룰라제의 효소작용을 향상시키는 단백질로서의 이용할 수 있다.
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