公开(公告)号：HK1161747A1

公开(公告)日：2012-08-03

申请号：HK12101838

申请日：2012-02-23

Applicant: FLUIDIGM CORP

Inventor： DAVID S COHEN S

IPC: C12Q20060101 ,  B01L20060101

公开(公告)号：SG152261A1

公开(公告)日：2009-05-29

申请号：SG2009027566

申请日：2005-01-25

Applicant: FLUIDIGM CORP

Inventor： GROSSMAN ROBERT ,  UNGER MARC ,  LAM PHILLIP ,  CHOU HOU-PU ,  KIMBALL JAKE ,  PIEPRZYK MARTIN

IPC: B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  C30B7/00 ,  C30B29/58

Abstract: The present invention provides for microfluidic devices and methods for their use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generated from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments, are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen against the microfluidic device so that at least one of the fluid ports of the platen is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure is changed therein.

公开(公告)号：CA2698545A1

公开(公告)日：2009-03-12

申请号：CA2698545

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: G01N33/48

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：AU2008295992A1

公开(公告)日：2009-03-12

申请号：AU2008295992

申请日：2008-09-08

Applicant: FLUIDIGM CORP

Inventor： RAMAKRISHNAN RAMESH

IPC: G01N33/48

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

公开(公告)号：AU2003224817B2

公开(公告)日：2008-11-06

申请号：AU2003224817

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： DARIDON ANTOINE ,  MANGER IAN D ,  LIAU YISH-HANN ,  NASSEF HANY RAMEZ ,  THRONDSET WILLIAM ,  CHOU HOU-PU ,  HAO CUNSHENG CASEY ,  NORTON PIERCE ,  FOWLER BRIAN ,  FARRELL KEVIN ,  JAVADI SHERVIN

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

公开(公告)号：CA2554240A1

公开(公告)日：2005-08-11

申请号：CA2554240

申请日：2005-01-25

Applicant: FLUIDIGM CORP

Inventor： PIEPRZYK MARTIN ,  KIMBALL JAKE ,  CHOU HOU-PU ,  UNGER MARC ,  LAM PHILLIP ,  GROSSMAN ROBERT

IPC: G01N1/28 ,  B01J19/00 ,  B01L3/00 ,  B01L3/06 ,  B01L9/00 ,  B01L99/00 ,  B81B1/00 ,  B81B7/00 ,  C30B7/00 ,  C30B29/58 ,  G01N35/02 ,  G01N37/00

Abstract: The present invention provides for microfluidic devices and methods for thei r use. The invention further provides for apparatus and systems for using the microfluidic devices, analyze reactions carried out in the microfluidic devices, and systems to generate, store, organize, and analyze data generate d from using the microfluidic devices. The invention further provides methods of using and making microfluidic systems and devices which, in some embodiments , are useful for crystal formation. In one embodiment, an apparatus includes a platen having a platen face with one or more fluid ports therein. The fluid ports spatially correspond to one or more wells on a surface of the microfluidic device. A platform for holding the microfluidic device relative to the platen is included, and a platen actuator for urging the platen again st the microfluidic device so that at least one of the fluid ports of the plate n is urged against one of the wells to form a pressure chamber comprising the well and the port, so that when pressurized fluid is introduced or removed into or from the pressure chamber through one of the ports, fluid pressure i s changed therein.

公开(公告)号：AU2004261655A1

公开(公告)日：2005-02-10

申请号：AU2004261655

申请日：2004-07-28

Applicant: FLUIDIGM CORP

Inventor： TAYLOR COLIN JON ,  DUBE SIMANT ,  SUN GANG

IPC: G06K9/00 ,  B01L3/00 ,  B29C20060101 ,  C12Q1/68 ,  G06K9/62 ,  G06T7/00

Abstract: A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

公开(公告)号：CA2526368A1

公开(公告)日：2004-12-02

申请号：CA2526368

申请日：2004-05-20

Applicant: FLUIDIGM CORP

Inventor： TAYLOR COLIN JON ,  LEE MICHAEL ,  SUN GANG ,  QUAN EMERSON CHUENG ,  CEASAR CHRISTOPHER G ,  HARRIS GREG

IPC: B01L3/00 ,  B29D11/00 ,  B44C1/22 ,  C02F1/40 ,  F15C1/04 ,  F15C1/06 ,  G01N21/00 ,  G01N33/558 ,  G01N35/02

Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

公开(公告)号：AU2003277853A8

公开(公告)日：2004-01-06

申请号：AU2003277853

申请日：2003-06-23

Applicant: FLUIDIGM CORP

Inventor： BARCO JOSEPH W ,  NASSEF HANY R ,  MANGER IAN D

IPC: B01L3/00 ,  B81C20060101 ,  F04B19/00 ,  F04B43/02 ,  G01N21/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/566 ,  G01N37/00

Abstract: A microfluidic device includes a plurality of first flow channels and a plurality of second flow channels, each such second flow channel intersecting multiple of the first flow channels to define intersecting volumes and a plurality of looped flow channels that each include segments of the flow channels between the intersecting volumes to define a closed loop. The microfluidic device also includes a plurality of control valves each such control valve having a control channel and a deformable segment disposed to restrict flow through a respective one of the first and second flow channels in response to an actuation force applied to the control channel to deflect the deformable segment. The microfluidic device further includes a pump operatively disposed to regulate flow through one of the looped flow channels to regulate flow by the recirculating pump.

公开(公告)号：SG11201609055RA

公开(公告)日：2016-11-29

申请号：SG11201609055R

申请日：2015-05-08

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN ,  CHARN TZE-HOWE ,  JOHNSON CHRISTIAN F ,  UNGER MARC A

IPC: C12Q1/68

Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.